The distribution of DMBA and its dihydrodiols in tissues of control and Sudan-III-treated Long—Evans rats after the injection (i.v.) in vivo of a leukaemogenic dose of the hydrocarbon

1988 ◽  
Vol 9 (1) ◽  
pp. 29-35 ◽  
Author(s):  
John J. O'Dowd ◽  
Alan K. Burnett
Keyword(s):  
Sudan Iii ◽  
Long Evans

scholarly journals Pharmacoinformatics and UPLC-QTOF/ESI-MS-Based Phytochemical Screening of Combretum indicum against Oxidative Stress and Alloxan-Induced Diabetes in Long–Evans Rats

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4634
Author(s):  
Md. Shaekh Forid ◽  
Md. Atiar Rahman ◽  
Mohd Fadhlizil Fasihi Mohd Aluwi ◽  
Md. Nazim Uddin ◽  
Tapashi Ghosh Roy ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Immune Modulation ◽  
Computational Study ◽  
Density Lipoprotein ◽  
Control Group ◽  
Esi Ms ◽  
Long Evans ◽  
Antidiabetic Effects

This research investigated a UPLC-QTOF/ESI-MS-based phytochemical profiling of Combretum indicum leaf extract (CILEx), and explored its in vitro antioxidant and in vivo antidiabetic effects in a Long–Evans rat model. After a one-week intervention, the animals’ blood glucose, lipid profile, and pancreatic architectures were evaluated. UPLC-QTOF/ESI-MS fragmentation of CILEx and its eight docking-guided compounds were further dissected to evaluate their roles using bioinformatics-based network pharmacological tools. Results showed a very promising antioxidative effect of CILEx. Both doses of CILEx were found to significantly (p < 0.05) reduce blood glucose, low-density lipoprotein (LDL), and total cholesterol (TC), and increase high-density lipoprotein (HDL). Pancreatic tissue architectures were much improved compared to the diabetic control group. A computational approach revealed that schizonepetoside E, melianol, leucodelphinidin, and arbutin were highly suitable for further therapeutic assessment. Arbutin, in a Gene Ontology and PPI network study, evolved as the most prospective constituent for 203 target proteins of 48 KEGG pathways regulating immune modulation and insulin secretion to control diabetes. The fragmentation mechanisms of the compounds are consistent with the obtained effects for CILEx. Results show that the natural compounds from CILEx could exert potential antidiabetic effects through in vivo and computational study.

Alterations in the metabolism of 7,12-dimethylbenz[a]anthracene and various xenobiotics by rat hepatic microsomes following Sudan III treatment in vivo

1985 ◽  
Vol 6 (3) ◽  
pp. 469-472 ◽  
Author(s):  
John J. O'Dowd ◽  
Alan K. Burnett ◽  
Ainsley Weston ◽  
Neil J. Bulleid ◽  
John A. Craft
Keyword(s):  
Hepatic Microsomes ◽  
Sudan Iii

Mechanism for mouse strain differences in the protective effect of Sudan III against the in vivo genotoxicity of 7,12-dimethylbenz[a]anthracene

1996 ◽  
Vol 89 (3) ◽  
pp. 231-239 ◽  
Author(s):  
Shigeki Hatakeyama ◽  
Yayoi Hayasaki ◽  
Makihiko Masuda ◽  
Akio Kazusaka ◽  
Shoichi Fujita
Keyword(s):  
Protective Effect ◽  
Mouse Strain ◽  
Strain Differences ◽  
Sudan Iii ◽  
Mouse Strain Differences

EFFECTS OF OESTRADIOL ON WATER UPTAKE AND α-AMINOISOBUTYRIC ACID ACCUMULATION BY UTERI OF HEPATECTOMIZED RATS

1963 ◽  
Vol 28 (1) ◽  
pp. 73-78 ◽  
Author(s):  
J. R. DANIELS ◽  
S. M. KALMAN
Keyword(s):  
Inferior Vena ◽  
Vena Cava ◽  
Early Response ◽  
Isobutyric Acid ◽  
Acid Accumulation ◽  
One Step ◽  
Long Evans ◽  
Amino Isobutyric Acid

SUMMARY Adult Long Evans rats were ovariectomized and, one month later, were subjected to partial hepatectomy. Immature Long Evans rats were either partially hepatectomized in one step, or complete hepatectomy was accomplished by a two-stage operation which included ligation of the inferior vena cava followed by a partial evisceration. The response of these animals to injected oestradiol was compared with that of sham-operated controls by observing uptake of water by the uterus in vivo, and also by estimating the ability of surviving uterine fragments to accumulate radioactive α-amino-isobutyric acid in vitro. It was found that partial or complete hepatectomy did not abolish the response of the uterus to oestradiol. These results seem to exclude an essential role for the liver in the early response of a target organ to an oestrogenic hormone.

Enterostatin inhibition of dietary fat intake is dependent on CCK-A receptors

2003 ◽  
Vol 285 (2) ◽  
pp. R321-R328 ◽  
Author(s):  
Ling Lin ◽  
Sonjya R. Thomas ◽  
Gail Kilroy ◽  
Gary J. Schwartz ◽  
David A. York
Keyword(s):  
Rat Brain ◽  
Receptor Gene ◽  
Fat Intake ◽  
Feeding Inhibition ◽  
Feeding Effect ◽  
Afferent Vagal ◽  
Long Evans ◽  
Dependent Pathway

Enterostatin, a pentapeptide released from the exocrine pancreas and gastrointestinal tract, selectively inhibits fat intake through activation of an afferent vagal signaling pathway. This study investigated if the effects of enterostatin were mediated through a CCK-dependent pathway. The series of in vivo and in vitro experiments included studies of 1) the feeding effect of peripheral enterostatin on Otsuka Long Evans Tokushima Fatty (OLETF) rats lacking CCK-A receptors, 2) the effect of CCK-8S on the intake of a two-choice high-fat (HF)/low-fat (LF) diet, 3) the effects of peripheral or central injection of the CCK-A receptor antagonist lorglumide on the feeding inhibition induced by either central or peripheral enterostatin, and 4) the ability of enterostatin to displace CCK binding in a 3T3 cell line expressing CCK-A receptor gene and in rat brain sections. The results showed that OLTEF rats did not respond to enterostatin (300 μg/kg ip) in contrast to the 23% reduction in intake of HF diet in Long Evans Tokushima Otsuka (LETO) control rats. CCK (1 μg/kg ip) decreased the intake of the HF diet in a two-choice diet regime with a compensatory increase in intake of the LF diet. Peripheral injection of lorglumide (300 μg/kg) blocked the feeding inhibition induced by either near-celiac arterial or intracerebroventricular enterostatin, whereas intracerebroventricular lorglumide (5 nmol icv) only blocked the response to intracerebroventricular enterostatin but not to arterial enterostatin. Enterostatin did not bind on CCK-A receptors because neither enterostatin nor its analogs VPDPR and β-casomorphin displaced [3H]L-364,718 from CCK-A receptors expressed in 3T3 cells or the binding of 125I-CCK-8S from rat brain sections. The data suggest that both the peripheral and central responses to enterostatin are mediated through or dependent on peripheral and central CCK-A receptors.

In vivo evidence for accelerated generation of hydroxyl radicals in liver of Long-Evans Cinnamon (LEC) rats with acute hepatitis

2001 ◽  
Vol 30 (5) ◽  
pp. 547-554 ◽  
Author(s):  
Hideki Yamamoto ◽  
Tomohiro Watanabe ◽  
Hatsue Mizuno ◽  
Kazuo Endo ◽  
Tsunemichi Hosokawa ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Hydroxyl Radicals ◽  
Lec Rats ◽  
Long Evans

scholarly journals The Dpg gene: an intracorpuscular modifier of red cell metabolism

Blood ◽  
1986 ◽  
Vol 67 (5) ◽  
pp. 1210-1214 ◽  
Author(s):  
NA Noble ◽  
G Rothstein
Keyword(s):  
Bone Marrow ◽  
Cell Survival ◽  
Cell Metabolism ◽  
Genetic Locus ◽  
Red Cell ◽  
Red Cell Metabolism ◽  
Red Cell Survival ◽  
Long Evans ◽  
Total Body

Abstract The genetic locus designated Dpg has two alleles in outbred Long-Evans rats. Genotype at this locus affects quantities of red cell 2,3- diphosphoglycerate (DPG) and adenosine triphosphate, as well as activities of two important glycolytic enzymes: phosphofructokinase and pyruvate kinase. Intravascular red cell survival is shortened in low- DPG animals. In order to get closer to the specific action of this locus, we addressed the question of whether the Dpg gene acts through intracorpuscular or extracorpuscular factors. Bone marrow transplantation after total body irradiation and 51Cr red cell survival after cross transfusion were the methods used. Because the animals that were used differed in hemoglobin phenotype, donor and recipient cells could be quantified in cross-transplanted animals. Phenotypic markers of Dpg genotype were measured in animals 40 to 50 days after transplantation. Values for these markers correlated highly with the percentage of donor and recipient cells present. In vivo survival of low-DPG red cells was significantly shorter than that of high-DPG cells (P less than .05), regardless of the genotype of the recipient. From the present studies, we conclude that the action of the Dpg gene is exerted by an intracorpuscular factor.

Sign in / Sign up

Export Citation Format

Share Document