scholarly journals Recovery of Undenatured Collagen Type II from Different Prototypes of Food and Beverages Using Enzyme-Linked Immunosorbent Assay (ELISA)

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 327-327
Author(s):  
Vijaya Juturu ◽  
George Pates ◽  
Robert Harris ◽  
Shane Durkee ◽  
Zainulabedin Saiyed
Keyword(s):  
Food Additives ◽  
Type Ii Collagen ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processing Temperature ◽  
Type Ii ◽  
Funding Sources ◽  
Food And Beverage ◽  
Joint Health ◽  
Undenatured Type Ii Collagen

Abstract Objectives According to a new report in 2020, the global functional food market size is projected to reach USD 275.77 billion by 2025 at a CAGR of 7.9%. One of the major growth drivers for this includes increasing demand for nutritional and fortifying food additives. Undenatured type II collagen (UC-II®) is a dietary ingredient derived from chicken sternum and has been shown to improve joint health. The development of undenatured type II collagen in different food and beverage (F&B) products is gaining momentum. The objective of this study was to assess the compatibility and recovery of undenatured type II collagen from different prototypes of F&B. Methods A specific ELISA test that detects the presence of native type II collagen was used to measure undenatured type II collagen content. Results Results showed the recovery of undenatured type II collagen from the F&B matrices varied depending on the pH, pressure, humidity and processing temperature. Highest recovery was seen from F&B prototype of nutritional bars (∼100%), chews (98%), gummies (96%), and dairy beverage (81%). Conclusions In summary, the results from this study shows that UC-II® is able to withstand the processing conditions used for manufacturing F&B products. The applicability of this findings will allow UC-II® to be incorporated into different functional foods thereby helping the consumers to improve their joint mobility, flexibility and comfort. Funding Sources Lonza Consumer Health Ingredients Inc.

scholarly journals Undenatured Type II Collagen (UC-II) in Joint Health and Disease: A Review on the Current Knowledge of Companion Animals

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 697 ◽  
Author(s):  
Hasan Gencoglu ◽  
Cemal Orhan ◽  
Emre Sahin ◽  
Kazim Sahin
Keyword(s):  
Current Knowledge ◽  
Companion Animals ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Cell Surfaces ◽  
Joint Health ◽  
Health And Disease ◽  
And Function ◽  
Undenatured Type Ii Collagen

OA is quite common in companion animals, especially in large breed dogs and horses. Collagen, the most abundant protein of mammals, has specific connective tissue types for skin, bones, reticulate, basal lamina, bones, cell surfaces, while type II collagen (UC-II) forms the main structure of cartilage tissue. Even at the smaller dosages, UC-II has also been reported to be more effective than the glucosamine and chondroitin sulfate supplements, which are the supplements most frequently used in the market. In this review, we summarize the effects of UC-II on joint health and function in health and disease conditions in companion animals.

scholarly journals Evaluation of Efficacy and Safety of NEXT-II®, a Novel Water-Soluble, Undenatured Type II Collagen in Subjects with Potential Risks in the Knee Joint Health from Healthy Population

2015 ◽  
Vol 5 (7) ◽  
pp. 251
Author(s):  
Orie Yoshinari ◽  
Hiroyoshi Moriyama ◽  
Yoshiaki Shiojima ◽  
Hiromi Miyawaki
Keyword(s):  
Knee Joint ◽  
Type Ii Collagen ◽  
Water Soluble ◽  
Healthy Population ◽  
Type Ii ◽  
Efficacy And Safety ◽  
Joint Health ◽  
Vas Scores ◽  
Potential Risks ◽  
Undenatured Type Ii Collagen

Background: Oral administration of a novel water-soluble undenatured type II collagen (NEXT-II®) has been demonstrated to ameliorate the signs and symptoms of rheumatoid arthritis (RA) in animal models. In the present investigation, we conducted a pilot study to examine the efficacy and safety of NEXT-II® in borderline subjects defined as healthy and non-diseased state, but with potential risks in knee joint health.  Method: We employed Western Ontario McMaster Index (WOMAC) score and Visual Analog Scale (VAS) scores to assess the extent of improvement in the knee joints in these volunteers following supplementation of 40 mg NEXT-II® (10 mg as undenatured type II collagen) over a period of 12 weeks.   Result: The results demonstrated that NEXT-II® treatment significantly reduced WOMAC and VAS scores compared to subjects at baseline. Specifically, in the evaluation using VAS, the borderline subjects at resting, walking, and going up and down the stairs revealed significant improvement when compared to the baseline.  Conclusion: The results of the studies demonstrated that NEXT-II® might be an ingredient which is safe and effective in the application of dietary supplement in ameliorating joint pain and symptoms of the borderline subjects without any adverse events. 

Correlation Between Muscle Strength and Functional Improvement After a Neuromuscular Electrical Strengthening Associated with Undenatured Type II Collagen in Knee Osteoarthritis

2021 ◽  
Vol 3 (5) ◽  
pp. 1122-1132
Author(s):  
Ana Paula Costa ◽  
Carlos Monteiro ◽  
Verine Cunha Teixeira ◽  
Bruno da Silva Schwarstzhoupt ◽  
Patrícia Mota Ferreira ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Knee Osteoarthritis ◽  
Type Ii Collagen ◽  
Functional Improvement ◽  
Type Ii ◽  
Undenatured Type Ii Collagen

Safety and toxicological evaluation of a novel, water-soluble undenatured type II collagen

2013 ◽  
Vol 23 (7) ◽  
pp. 491-499 ◽  
Author(s):  
Orie Yoshinari ◽  
Palma Ann Marone ◽  
Hiroyoshi Moriyama ◽  
Manashi Bagchi ◽  
Yoshiaki Shiojima
Keyword(s):  
Type Ii Collagen ◽  
Water Soluble ◽  
Type Ii ◽  
Toxicological Evaluation ◽  
Undenatured Type Ii Collagen

scholarly journals Undenatured type II collagen (UC-II®) for joint support: a randomized, double-blind, placebo-controlled study in healthy volunteers

2013 ◽  
Vol 10 (1) ◽  
pp. 48 ◽  
Author(s):  
James P Lugo ◽  
Zainulabedin M Saiyed ◽  
Francis C Lau ◽  
Jhanna Pamela L Molina ◽  
Michael N Pakdaman ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Type Ii Collagen ◽  
Controlled Study ◽  
Type Ii ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Undenatured Type Ii Collagen

scholarly journals Effect of Marine Phytoplankton (Oceanix) and Undenatured Type II Collagen in Combination with Exercise on Endurance Capacity and Oxidative Stress in Rats

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1764-1764
Author(s):  
Kazim Sahin ◽  
Cemal Orhan ◽  
Mehmet Tuzcu ◽  
Nurhan Sahin ◽  
Vijaya Juturu
Keyword(s):  
Antioxidant Enzymes ◽  
Proinflammatory Cytokines ◽  
Type Ii Collagen ◽  
Marine Phytoplankton ◽  
Albino Rats ◽  
High Dose ◽  
Endurance Capacity ◽  
Type Ii ◽  
Tnf Α ◽  
Undenatured Type Ii Collagen

Abstract Objectives To study the effect of exercise training alone and or in combination with marine phytoplankton (Oceanix, OCX) and undenatured type II collagen (UCII) supplementation on the endurance capacity, pro-inflammatory markers, and antioxidant defense markers in rats. Methods A total of 28 male Wistar albino rats were randomly divided into four groups (n  =  7) (i) No exercise and no OCX (Control), (ii) Exercise, (iii) Exercise +OCX-I (2.55 mg d/rat) + UC-II (4 mg), iv) Exercise + OCX-2 (5.1 mg d/rat)+UC-II (4 mg). Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP), lactate and malondialdehyde (MDA) levels activities of antioxidant enzymes were determined in all the groups. Results Run to exhaustion (minutes) improved in the OCX + UC-II treated groups. Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP) decreased by OCX + UC-II supplementation. A significant decrease in lactate and malondialdehyde (MDA) levels and an increase in activities of antioxidant enzymes were observed in the combination of exercise and OCX + UC-II groups. Exercise + OCX + UC-II treated had lower TNF-α and IL-1β levels in muscle than exercise and control rats (P < 0.001). Muscle sterol regulatory element-binding protein 1c (SREBP-1c), liver X receptors (LXR), ATP citrate lyase (ACLY) and fatty acid synthase (FAS) levels in the exercise + OCX + UC-II group were lower than all groups (P < 0.05). The effectiveness of the high dose of OCX was more pronounced than the low dose of OCX. Conclusions These results suggest OCX and UC-II with exercise may enhance lipid metabolism by regulation of gene products involved in lipid and antioxidant metabolism including SREBP-1c, -γ, LXR, ACLY and FAS in rats. Funding Sources Lonza.

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