Correlation Between Muscle Strength and Functional Improvement After a Neuromuscular Electrical Strengthening Associated with Undenatured Type II Collagen in Knee Osteoarthritis

2021 ◽  
Vol 3 (5) ◽  
pp. 1122-1132
Author(s):  
Ana Paula Costa ◽  
Carlos Monteiro ◽  
Verine Cunha Teixeira ◽  
Bruno da Silva Schwarstzhoupt ◽  
Patrícia Mota Ferreira ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Knee Osteoarthritis ◽  
Type Ii Collagen ◽  
Functional Improvement ◽  
Type Ii ◽  
Undenatured Type Ii Collagen

scholarly journals Functional Characterization of Undenatured Type II Collagen Supplements: Are They Interchangeable?

2021 ◽  
pp. 1-16
Author(s):  
Robert B. Harris ◽  
Fernando L. A Fonseca ◽  
Matthew H. Sharp ◽  
Charlie R. Ottinger
Keyword(s):  
Functional Characterization ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Undenatured Type Ii Collagen

scholarly journals A Novel High Sensitivity Type II Collagen Blood-Based Biomarker, PRO-C2, for Assessment of Cartilage Formation

2018 ◽  
Vol 19 (11) ◽  
pp. 3485 ◽  
Author(s):  
Yunyun Luo ◽  
Yi He ◽  
Ditte Reker ◽  
Natasja Gudmann ◽  
Kim Henriksen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Growth Factor ◽  
Knee Osteoarthritis ◽  
High Sensitivity ◽  
Type Ii Collagen ◽  
Cartilage Explants ◽  
Type Ii ◽  
Serum Samples ◽  
Human Cartilage ◽  
Cartilage Formation

N-terminal propeptide of type II collagen (PIINP) is a biomarker reflecting cartilage formation. PIINP exists in two main splice variants termed as type IIA and type IIB collagen NH2-propeptide (PIIANP, PIIBNP). PIIANP has been widely recognized as a cartilage formation biomarker. However, the utility of PIIBNP as a marker in preclinical and clinical settings has not been fully investigated yet. In this study, we aimed to characterize an antibody targeting human PIIBNP and to develop an immunoassay assessing type II collagen synthesis in human blood samples. A high sensitivity electrochemiluminescence immunoassay, hsPRO-C2, was developed using a well-characterized antibody against human PIIBNP. Human cartilage explants from replaced osteoarthritis knees were cultured for ten weeks in the presence of growth factors, insulin-like growth factor 1 (IGF-1) or recombinant human fibroblast growth factor 18 (rhFGF-18). The culture medium was changed every seven days, and levels of PIIBNP, PIIANP, and matrix metalloproteinase 9-mediated degradation of type II collagen (C2M) were analyzed herein. Serum samples from a cross-sectional knee osteoarthritis cohort, as well as pediatric and rheumatoid arthritis samples, were assayed for PIIBNP and PIIANP. Western blot showed that the antibody recognized PIIBNP either as a free fragment or attached to the main molecule. Immunohistochemistry demonstrated that PIIBNP was predominately located in the extracellular matrix of the superficial and deep zones and chondrocytes in both normal and osteoarthritic articular cartilage. In addition, the hsPRO-C2 immunoassay exhibits acceptable technical performances. In the human cartilage explants model, levels of PIIBNP, but not PIIANP and C2M, were increased (2 to 7-fold) time-dependently in response to IGF-1. Moreover, there was no significant correlation between PIIBNP and PIIANP levels when measured in knee osteoarthritis, rheumatoid arthritis, and pediatric serum samples. Serum PIIBNP was significantly higher in controls (KL0/1) compared to OA groups (KL2/3/4, p = 0.012). The hsPRO-C2 assay shows completely different biological and clinical patterns than PIIANP ELISA, suggesting that it may be a promising biomarker of cartilage formation.

Safety and toxicological evaluation of a novel, water-soluble undenatured type II collagen

2013 ◽  
Vol 23 (7) ◽  
pp. 491-499 ◽  
Author(s):  
Orie Yoshinari ◽  
Palma Ann Marone ◽  
Hiroyoshi Moriyama ◽  
Manashi Bagchi ◽  
Yoshiaki Shiojima
Keyword(s):  
Type Ii Collagen ◽  
Water Soluble ◽  
Type Ii ◽  
Toxicological Evaluation ◽  
Undenatured Type Ii Collagen

scholarly journals Undenatured type II collagen (UC-II®) for joint support: a randomized, double-blind, placebo-controlled study in healthy volunteers

2013 ◽  
Vol 10 (1) ◽  
pp. 48 ◽  
Author(s):  
James P Lugo ◽  
Zainulabedin M Saiyed ◽  
Francis C Lau ◽  
Jhanna Pamela L Molina ◽  
Michael N Pakdaman ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Type Ii Collagen ◽  
Controlled Study ◽  
Type Ii ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Undenatured Type Ii Collagen

scholarly journals The relationship between urinary C-Telopeptide fragments of type II collagen, knee joint load, pain, and physical function in individuals with medial knee osteoarthritis

2020 ◽  
Author(s):  
Luiz Fernando Approbato Selistre ◽  
Glaucia Helena Gonçalves ◽  
Fernando Augusto Vasilceac ◽  
Paula Regina Mendes da Silva Serrão ◽  
Theresa Helissa Nakagawa ◽  
...  
Keyword(s):  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Physical Function ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Medial Knee ◽  
Medial Knee Osteoarthritis ◽  
Knee Joint Load ◽  
Joint Load ◽  
The Relationship

scholarly journals Effect of Marine Phytoplankton (Oceanix) and Undenatured Type II Collagen in Combination with Exercise on Endurance Capacity and Oxidative Stress in Rats

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1764-1764
Author(s):  
Kazim Sahin ◽  
Cemal Orhan ◽  
Mehmet Tuzcu ◽  
Nurhan Sahin ◽  
Vijaya Juturu
Keyword(s):  
Antioxidant Enzymes ◽  
Proinflammatory Cytokines ◽  
Type Ii Collagen ◽  
Marine Phytoplankton ◽  
Albino Rats ◽  
High Dose ◽  
Endurance Capacity ◽  
Type Ii ◽  
Tnf Α ◽  
Undenatured Type Ii Collagen

Abstract Objectives To study the effect of exercise training alone and or in combination with marine phytoplankton (Oceanix, OCX) and undenatured type II collagen (UCII) supplementation on the endurance capacity, pro-inflammatory markers, and antioxidant defense markers in rats. Methods A total of 28 male Wistar albino rats were randomly divided into four groups (n  =  7) (i) No exercise and no OCX (Control), (ii) Exercise, (iii) Exercise +OCX-I (2.55 mg d/rat) + UC-II (4 mg), iv) Exercise + OCX-2 (5.1 mg d/rat)+UC-II (4 mg). Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP), lactate and malondialdehyde (MDA) levels activities of antioxidant enzymes were determined in all the groups. Results Run to exhaustion (minutes) improved in the OCX + UC-II treated groups. Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP) decreased by OCX + UC-II supplementation. A significant decrease in lactate and malondialdehyde (MDA) levels and an increase in activities of antioxidant enzymes were observed in the combination of exercise and OCX + UC-II groups. Exercise + OCX + UC-II treated had lower TNF-α and IL-1β levels in muscle than exercise and control rats (P < 0.001). Muscle sterol regulatory element-binding protein 1c (SREBP-1c), liver X receptors (LXR), ATP citrate lyase (ACLY) and fatty acid synthase (FAS) levels in the exercise + OCX + UC-II group were lower than all groups (P < 0.05). The effectiveness of the high dose of OCX was more pronounced than the low dose of OCX. Conclusions These results suggest OCX and UC-II with exercise may enhance lipid metabolism by regulation of gene products involved in lipid and antioxidant metabolism including SREBP-1c, -γ, LXR, ACLY and FAS in rats. Funding Sources Lonza.

Sign in / Sign up

Export Citation Format

Share Document