scholarly journals Multielemental Fractionation in Human Peripheral Blood Mononuclear Cells by Size Exclusion Liquid Chromatography Coupled to UV and ICP-MS Detection

2010 ◽  
Vol 48 (9) ◽  
pp. 697-703 ◽  
Author(s):  
G. Alvarado ◽  
M. Murillo
Keyword(s):  
Liquid Chromatography ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Size Exclusion ◽  
Peripheral Blood Mononuclear ◽  
Ms Detection ◽  
Icp Ms ◽  
Blood Mononuclear Cells

scholarly journals A Novel Factor Isolated from Actinobacillus actinomycetemcomitans Stimulates Mouse B Cells and Human Peripheral Blood Mononuclear Cells

2000 ◽  
Vol 68 (9) ◽  
pp. 5132-5138 ◽  
Author(s):  
Soo-Jin Jeong ◽  
Sung-Tae Yee ◽  
Wol-Soon Jo ◽  
Seong-Ho Yu ◽  
Sang-Hwa Lee ◽  
...  
Keyword(s):  
B Cells ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Actinobacillus Actinomycetemcomitans ◽  
Size Exclusion ◽  
Soluble Form ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

ABSTRACT A novel immunostimulating factor (ISTF) of Actinobacillus actinomycetemcomitans ATCC 29522 was isolated and characterized as inducing proliferation of mouse B cells and human peripheral blood mononuclear cells. This factor was isolated from the bacterial culture medium and purified by size exclusion chromatography, dye-ligand affinity chromatography, immunoaffinity chromatography using monoclonal antibodies, and preparative electrophoresis. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the purified ISTF migrated as a single band corresponding to a molecular mass of 13 kDa. ISTF was a proteinaceous material distinct from lipopolysaccharide; it directly induced the proliferation of B lymphocytes but had no effect on the proliferation of T lymphocytes, even in the presence of antigen-presenting cells. A B-lymphocyte-mitogenic activity of ISTF was also shown by flow cytometric analysis of responding cell subpopulations. Immunoblot analysis revealed that ISTF was a component of the outer membranes of bacteria, could exist as a soluble form, and was released by growing and/or lysed bacteria. These results suggest that ISTF produced byA. actinomycetemcomitans may play an important role in immunopathologic changes associated with A. actinomycetemcomitans infections.

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