Simultaneous Determination of Eight Components in Leonuri Herba from Different Habitats by HPLC-DAD

2020 ◽  
Vol 58 (5) ◽  
pp. 464-470
Author(s):  
Yang Xu ◽  
Huailei Yang ◽  
Jihong Chi ◽  
Fang Wang ◽  
Huiwei Bao
Keyword(s):  
Quality Control ◽  
Phosphoric Acid ◽  
Acid Solution ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Phosphoric Acid Solution ◽  
Linear Relationships ◽  
Ethanol Extracts

Abstract A HPLC-DAD method was established for the simultaneous determination of eight components in Leonuri Herba from different habitats, as well as providing methodological reference for quality control of Leonuri Herba. In this study, absolute ethanol extracts of the medicinal material were considered as the sample solutions to be analyzed. Agilent ZORBAX SB-C18 column (250 mm × 4.6 mm, 5 μm) was used for determination maintained at the temperature of 30°C. Gradient elution was performed with a mobile phase of methanol (B)-0.1% phosphoric acid solution (A) at the flow rate of 1.0 mL·min−1. The analysis was carried out at the wavelength of 225, 280 and 320 nm. The measured eight components showed good linear relationships within their own concentration range. Average recoveries ranged from 98.83 to 100.36% with RSDs of 1.14~1.97%. The proposed method was found to be simple, accurate and reproducible, which provided an effective quantitative analytical method for quality control of Leonuri Herba from different habitats.

Determination of Paeoniflorin and Paeonol in Houyinan Tablet by UPLC

2012 ◽  
Vol 581-582 ◽  
pp. 68-72
Author(s):  
Chu Qin Yu ◽  
Hua Qing Lin ◽  
Yue Han Hou ◽  
Zhong Feng Shi ◽  
Di Shi Lin
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Mobile Phase ◽  
Gradient Elution ◽  
The Other ◽  
Column Temperature ◽  
Average Recovery ◽  
Injection Volume

In this study, our purpose was to establish a UPLC method for the simultaneous determination of Paeoniflorin and Paeonol in Houyinan Tablet. The separation was performed on Acquity BEH C18 column(2.1mm×100mm,1.7μm), the mobile phase was acetonitrile-water with gradient elution at a flow rate of 0.2 mL•min-1, the detection wavelength was 230nm, the column temperature was 30°Cand the injection volume was 2μL. Paeoniflorin and Paeonol reached effective separation with the other components in this chromatographic conditions. Paeoniflorin and Paeonol were linear within the range of 0.0406~0.4064μg(r=0.9999) and 0.0426~0.4256μg (r=0.9999), respectively. The average recovery was 99.82% and 100.6%. The results of method validation indicated that the method was simple,quick,accurate, specific and less solvent consumption. It can be used for the quality control of Houyinan Tablet.

scholarly journals Simultaneous quantification of eurycomanone, epimedin C and icariin in film coated tablets containing Radix Eurycomae longifoliae extract and Herba Epimedii extract using HPLC – DAD

Pharmacia ◽  
2021 ◽  
Vol 68 (2) ◽  
pp. 365-373
Author(s):  
Huynh Tran Quoc Dung ◽  
Pham Ngoc Thac ◽  
Nguyen Ngoc Thach ◽  
Nguyen Phuong Nam ◽  
Nguyen Duc Hanh
Keyword(s):  
Quality Control ◽  
Sample Preparation ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Simultaneous Quantification ◽  
Preparation Conditions ◽  
Herba Epimedii ◽  
First Time

Introduction. This study aimed at developing an HPLC-DAD method for simultaneous determination of eurycomanone (EU), epimedin C (EC) and icariin (IC) in EE tablets containing Radix Eurycomae longifoliae extract and Herba Epimedii extract (EE tablets). Methods. Several HPLC conditions (detection wavelength, mobile phase) and sample preparation conditions (type of solvent, ratios of tablet powder and solvent) were investigated. Results. The optimized HPLC condition employed a Shim-Pack RP18 column and a detection wavelength of 254 nm. The mobile phase consisted of acetonitrile and water containing 0.1% H3PO4 in gradient elution. The method was selective, linear, precise (RSD < 2%) and accurate with recoveries in the range of 98.51–104.58% for EU, 96.22–104.80% for EC and 97.50–104.96% for IC. Conclusion. The method for simultaneous quantification of EU, EC and IC in EE tablets by HPLC – DAD was developed and validated for the first time and could be applied for quality control of EE tablets.

Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS

2020 ◽  
Vol 17 (1) ◽  
pp. 47-56
Author(s):  
Shun Liu ◽  
Xun Wang ◽  
Kaiping Zou ◽  
Wei Liu ◽  
Cunyu Li ◽  
...  
Keyword(s):  
Quality Control ◽  
Chinese Medicine ◽  
Simultaneous Determination ◽  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Quality Markers ◽  
High Repeatability ◽  
Comprehensive Study

Background: Zishen Tongguan (ZSTG) capsules were prepared at the Affiliated Hospital of Nanjing University of Chinese Medicine and have been proven to be clinically effective for treating pyelonephritis and benign prostatic hyperplasia. However, the quality standards are not ideal; a comprehensive study of the “quality markers” (Q-markers), the chemicals inherent in traditional Chinese medicine and its preparations, has not been carried out. Experimental Methods: In this paper, a sensitive and specific ultra-high-performance liquid chromatographictandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of eight potential Q-markers of ZSTG, including timosaponin A3, berberine, jatrorrhizine, phellodendrine, palmatine, mangiferin, neomangiferin, and timosaponin BII. A Kromasil 100-3.5 C18 column was used with a mobile phase of 0.2% formic acid with acetonitrile, and gradient elution at a flow rate of 0.2 mL/min was achieved in 13 minutes and used for separation. Detection was performed in positive/negative mode with multiple reaction monitoring (MRM). Results: The analytical method was validated in terms of the sensitivity, linearity, accuracy, precision, repeatability, stability and recovery. The method established here was successfully applied to study the potential Q-markers in 8 batches of commercial samples, which demonstrated its use in improving the quality control of ZSTG. Conclusion: The developed method had high repeatability and accuracy and was suitable for the simultaneous analysis of multiple Q-markers, which may provide a new basis for the comprehensive assessment and overall quality control of ZSTG.

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

Simultaneous Determination of Seven Active Components in Desheng Pills by High-performance Liquid Chromatography

2020 ◽  
Vol 17 (1) ◽  
pp. 149-158
Author(s):  
Yang Xu ◽  
Huailei Yang ◽  
Baiyu Shan ◽  
Kuo Fang ◽  
Mingyu Li ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Blood Stasis ◽  
Gradient Elution ◽  
Phosphoric Acid Solution ◽  
Array Detector ◽  
Active Components

Background: Desheng pills (DSP) consist of six traditional Chinese medicine. This preparation is used fornourishing blood, eliminating stasis, soothing liver and regulating menstruation, and can also be used to treat menoxenia and dysmenorrhea caus ed by qi stagnation and blood stasis. Objective: In this paper, an accurate and sensitive high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed and validated for simultaneous determination of seven active components (gallic acid, paeoniflorin, costunolide, dehydrocostuslactone, rutin, leonurine hydrochloride and ferulic acid) in the traditional Chinese formula-Desheng pills. Methods: The seven analytes were separated on Agilent ZORBAX SB-C18 column (250mm× 4.6mm, 5μm) maintained at the temperature of 30.. Gradient elution was performed with the mobile phase of methanol (A)-0.1% phosphoric acid solution (B) at the flow rate of 1.0mL·min-1. The analysis was carried out at the wavelength of 225 nm, 256 nm, 277 nm and 320 nm with an injection volume of 10 μL. Results: The measured seven components showed good linear relationships within their own concentration ranges along with coefficients of determination ≥0.9996. The limits of detection and quantitation of all analytes were in the range of 0.19-13.51 μg/mL and 0.59-40.93 μg/mL, respectively. Average recoveries ranged from 98.82% to 102.01% with RSDs of 1.47%-1.99%. The content of tested components was in the range of 0.053-0.421 mg/g. Conclusion: The proposed method was found to be sensitive, accurate and reproducible, which provided an effective quantitative analytical method for quality control of Desheng pills.

scholarly journals Simultaneous Determination of 5 Flavonoids and 7 Saponins for Quality Control of Traditional Chinese Medicine Preparation Xinnaoshutong Capsule Using HPLC-VWD-ELSD

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Jin Li ◽  
Yang Bai ◽  
Peng Zhang ◽  
Jun He ◽  
Jun Cao ◽  
...  
Keyword(s):  
Quality Control ◽  
Analytical Method ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Tribulus Terrestris ◽  
Ripe Fruit ◽  
Accuracy And Precision ◽  
Marker Compounds ◽  
Traditional Chinese Medicine Preparation

Xinnaoshutong capsule (XC) is a traditional Chinese prescription derived from the ripe fruit of Tribulus terrestris L. (TT). Although XC has long been considered as an important herbal medicine, no analytical method of marker compounds for quality assessment is registered in the Chinese Pharmacopoeia. A simple analytical method of twelve marker components was developed and validated by HPLC-VWD-ELSD method. Chromatographic separation by HPLC was carried out on a Hedera ODS 2 column (4.6 × 250 mm, 5 μm) by gradient elution with acetonitrile-water (0.1% formic acid) as the mobile phase. Various extraction conditions were optimized to achieve twelve marker compounds with faster extraction and higher recovery. The analytical condition was then validated in terms of the linearity, accuracy and precision, repeatability, and stability. The twelve markers were successfully quantified in 30 batches of commercial samples. The developed HPLC-VWD-ELSD could be used as a rapid and reliable way in the assessment and quality control of XC and TT.

Development of gamma spectrometric method for the determination of thorium in phosphoric acid solution

2010 ◽  
Vol 98 (5) ◽  
Author(s):  
N. N. Mirashi ◽  
Satyajeet Chaudhury ◽  
S. K. Aggarwal
Keyword(s):  
Phosphoric Acid ◽  
Acid Solution ◽  
High Purity ◽  
Hpge Detector ◽  
Phosphoric Acid Solution ◽  
Spectrometric Method ◽  
High Purity Germanium

AbstractGamma spectrometric determination of thorium in sintered thoria dissolved in strong phosphoric acid was studied using a high purity germanium (HPGe) detector.

Collaborative Study of a Spectrofluorometric Assay for Rauwolfia serpentina Tablets and Powdered Root, Using a Vanadium Pentoxide-Phosphoric Acid Determinative Step

1977 ◽  
Vol 60 (5) ◽  
pp. 1018-1021 ◽  
Author(s):  
William M Smith
Keyword(s):  
Phosphoric Acid ◽  
Acid Solution ◽  
Silica Gel ◽  
Vanadium Pentoxide ◽  
Collaborative Study ◽  
Chloroform Extract ◽  
Phosphoric Acid Solution ◽  
Rauwolfia Serpentina ◽  
Chloroform Methanol

Abstract Reserpine-rescinnamine group alkaloids are extracted from Rauwolfia serpentina preparations into dimethylsulfoxide (DMSO)-methanol and diluted with 0.5N H2SO4. The chloroform extract of this solution is passed through a 0.1N NaOH-Celite column and then through a silica gel column. The weakly basic alkaloids trapped on the latter column are eluted with chloroform-methanol; a portion of the eluate is treated with a vanadium pentoxide-phosphoric acid solution, and the reserpine-rescinnamine content is determined by measuring the intensity of the resulting fluorescence. The following means and standard deviations (11 collaborators) were obtained for the determination of reserpine-rescinnamine group alkaloids in 4 samples of Rauwolfia serpentina (NF reference powder, 100 and 50 mg commercial tablets, and 45 mg synthetic tablet formulation) : 0.173%, 0.0050; 0.128%, 0.0030; 0.150%, 0.0069; and 0.142%, 0.0032; respectively. The method has been adopted as official first action.

scholarly journals Simultaneous Determination of Rutin, Luteolin, Quercetin, and Betulinic Acid in the Extract ofDisporopsis pernyi(Hua) Diels by UPLC

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Yanqi Wang ◽  
Shuyi Li ◽  
Dandan Han ◽  
Kehan Meng ◽  
Miao Wang ◽  
...  
Keyword(s):  
Quality Control ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
Betulinic Acid ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Relative Standard ◽  
Quantitative Basis

Disporopsis pernyi(Hua) Diels, which belongs to genusDisporopsis, has been widely used for the treatment of abnormal sweating, chronic cough, and so forth. An ultra-performance liquid chromatography (UPLC) analysis was developed for the determination of rutin, luteolin, quercetin, and betulinic acid inDisporopsis pernyi(Hua) Diels roots. UPLC analysis was conducted by using a Shim-pack XR-ODS column with gradient elution with the mobile phase of acetonitrile and water containing 0.1% formic acid and with a flow rate of 0.2 mL/min, detected at 210, 254, and 280 nm. The method was precise, with relative standard deviation < 2.0%. The recoveries for the four components inDisporopsis pernyi(Hua) Diels were between 98.5 and 100.9%. The average contents of rutin, luteolin, quercetin, and betulinic acid in roots were 5.63, 2.51, 3.87, and 2.41 μg/g, respectively. The method was accurate and reproducible and it can provide a quantitative basis for quality control ofDisporopsis pernyi(Hua) Diels.

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