Features of Infections Due to Klebsiella pneumoniae Carbapenemase–Producing Escherichia coli: Emergence of Sequence Type 131

Abstract We characterized 57 isolates from a 2-phase clonal outbreak of New Delhi metallo-β-lactamase–producing Eschericha coli, involving 9 Israeli hospitals; all but 1 isolate belonged to sequence-type (ST) 410. Most isolates in the second phase harbored blaKPC-2 in addition to blaNDM-5. Genetic sequencing revealed most dual-carbapenemase–producing isolates to be monophyletically derived from a common ancestor.

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Transmission of novel Klebsiella pneumoniae carbapenemase-producing Escherichia coli sequence type 1193 among residents and caregivers in a community-based, residential care setting—Nevada, 2018

Infection Control and Hospital Epidemiology ◽

10.1017/ice.2020.279 ◽

2020 ◽

Vol 41 (11) ◽

pp. 1341-1343

Author(s):

Danica J. Gomes ◽

Ana C. Bardossy ◽

Lei Chen ◽

Adrian Forero ◽

Andrew Gorzalski ◽

...

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Group Home ◽

The United States ◽

Multidrug Resistant ◽

Resistance Mechanisms ◽

Sequence Type ◽

E Coli ◽

Residential Group ◽

Klebsiella Pneumoniae Carbapenemase

AbstractWe describe transmission of Klebsiella pneumoniae carbapenemase-producing Escherichia coli sequence type (ST) 1193 in a group home. E. coli ST1193 is an emerging multidrug-resistant clone not previously shown to carry carbapenemases in the United States. Our investigation illustrates the potential of residential group homes to amplify rare combinations of pathogens and resistance mechanisms.

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Faculty Opinions recommendation of A 4.5-Year Within-Patient Evolution of a Colistin-Resistant Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Sequence Type 258.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733092378.793545532 ◽

2018 ◽

Author(s):

Rasmus Leistner

Keyword(s):

Klebsiella Pneumoniae ◽

Sequence Type ◽

Klebsiella Pneumoniae Carbapenemase

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A 4.5-Year Within-Patient Evolution of a Colistin-Resistant Klebsiella pneumoniae Carbapenemase–Producing K. pneumoniae Sequence Type 258

Clinical Infectious Diseases ◽

10.1093/cid/ciy293 ◽

2018 ◽

Vol 67 (9) ◽

pp. 1388-1394 ◽

Cited By ~ 11

Author(s):

Agnès B Jousset ◽

Rémy A Bonnin ◽

Isabelle Rosinski-Chupin ◽

Delphine Girlich ◽

Gaëlle Cuzon ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Sequence Type ◽

Klebsiella Pneumoniae Carbapenemase

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Draft Genome Sequence of a Klebsiella pneumoniae Carbapenemase-Positive Sequence Type 111 Pseudomonas aeruginosa Strain

Genome Announcements ◽

10.1128/genomea.01663-15 ◽

2016 ◽

Vol 4 (1) ◽

Cited By ~ 3

Author(s):

Gabrielle A. Dotson ◽

John P. Dekker ◽

Tara N. Palmore ◽

Julia A. Segre ◽

Sean Conlan ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Genome Sequence ◽

Draft Genome ◽

Sequence Type ◽

Draft Genome Sequence ◽

Positive Sequence ◽

Gene Encoding ◽

Klebsiella Pneumoniae Carbapenemase ◽

Clinical Center

Here, we report the draft genome sequence of a sequence type 111 Pseudomonas aeruginosa strain isolated in 2014 from a patient at the NIH Clinical Center. This P. aeruginosa strain exhibits pan-drug resistance and harbors the bla KPC-2 gene, encoding the Klebsiella pneumoniae carbapenemase enzyme, on a plasmid.

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Risk Factors for Mortality in Patients with Klebsiella pneumoniae Carbapenemase-producing K. pneumoniae and Escherichia coli bacteremia

Infection and Chemotherapy ◽

10.3947/ic.2021.0083 ◽

2021 ◽

Vol 53 (3) ◽

pp. 528

Author(s):

Hyeonji Seo ◽

Seongman Bae ◽

Min Jae Kim ◽

Yong Pil Chong ◽

Sung-Han Kim ◽

...

Keyword(s):

Risk Factors ◽

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Klebsiella Pneumoniae Carbapenemase

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Cooccurrence of NDM-1, ESBL, RmtC, AAC(6′)-Ib, and QnrB in Clonally Related Klebsiella pneumoniae Isolates Together with Coexistence of CMY-4 and AAC(6′)-Ib in Enterobacter cloacae Isolates from Saudi Arabia

BioMed Research International ◽

10.1155/2019/6736897 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Mohamed H. Al-Agamy ◽

Taghrid S. El-Mahdy ◽

Hesham H. Radwan ◽

Laurent Poirel

Keyword(s):

Escherichia Coli ◽

Saudi Arabia ◽

16S Rrna ◽

Klebsiella Pneumoniae ◽

High Resistance ◽

Enterobacter Cloacae ◽

Sequence Type ◽

Quinolone Resistance ◽

Synergy Test ◽

16S Rrna Methylase

The aim of this study was to investigate the mechanisms responsible for resistance to antimicrobials in a collection of enterobacterial isolates recovered from two hospitals in Saudi Arabia. A total of six strains isolated from different patients showing high resistance to carbapenems was recovered in 2015 from two different hospitals, with four being Klebsiella pneumoniae and two Enterobacter cloacae. All isolates except one K. pneumoniae were resistant to tigecycline, but only one K. pneumoniae was resistant to colistin. All produced a carbapenemase according to the Carba NP test, and all were positive for the EDTA-disk synergy test for detection of MBL. Using PCR followed by sequencing, the four K. pneumoniae isolates produced the carbapenemase NDM-1, while the two E. cloacae isolates produced the carbapenemase VIM-1. Genotyping analysis by Multilocus Sequence Typing (MLST) showed that three out of the four K. pneumoniae isolates were clonally related. They had been recovered from the same hospital and belonged to Sequence Type (ST) ST152. In contrast, the fourth K. pneumoniae isolate belonged to ST572. Noticeably, the NDM-1-producing K. pneumoniae additionally produced an extended-spectrum ß-lactamase (ESBL) of the CTX-M type, together with OXA-1 and TEM-1. Surprisingly, the three clonally related isolates produced different CTX-M variants, namely, CTX-M-3, CTX-M-57, and CTX-M-82, and coproduced QnrB, which confers quinolone resistance, and the 16S rRNA methylase RmtC, which confers high resistance to all aminoglycosides. The AAC(6′)-Ib acetyltransferase was detected in both K. pneumoniae and E. cloacae. Mating-out assays using Escherichia coli as recipient were successful for all isolates. The blaNDM-1 gene was always identified on a 70-kb plasmid, whereas the blaVIM-1 gene was located on either a 60-kb or a 150-kb plasmid the two E. cloacae isolates, respectively. To the best of our knowledge, this is the first report of the coexistence of an MBL (NDM-1), an ESBL (CTX-M), a 16S rRNA methylase (RmtC), an acetyltransferase (AAC[6′]-Ib), and a quinolone resistance enzyme (QnrB) in K. pneumoniae isolates recovered from different patients during an outbreak in a Saudi Arabian hospital.

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An Emerging Clone, Klebsiellapneumoniae Carbapenemase 2–Producing K. pneumoniae Sequence Type 16, Associated With High Mortality Rates in a CC258-Endemic Setting

Clinical Infectious Diseases ◽

10.1093/cid/ciz1095 ◽

2019 ◽

Vol 71 (7) ◽

pp. e141-e150 ◽

Cited By ~ 4

Author(s):

Diego O Andrey ◽

Priscila Pereira Dantas ◽

Willames B S Martins ◽

Fabíola Marques De Carvalho ◽

Luiz Gonzaga Paula Almeida ◽

...

Keyword(s):

Risk Factors ◽

Klebsiella Pneumoniae ◽

High Mortality ◽

Fatal Outcome ◽

Mortality Rates ◽

Sequence Type ◽

Virulence Determinants ◽

Klebsiella Pneumoniae Carbapenemase ◽

Endemic Setting ◽

Severity Scores

Abstract Background Carbapenemase-producing Klebsiella pneumoniae has become a global priority, not least in low- and middle-income countries. Here, we report the emergence and clinical impact of a novel Klebsiella pneumoniae carbapenemase–producing K. pneumoniae (KPC-KP) sequence type (ST) 16 clone in a clonal complex (CC) 258–endemic setting. Methods In a teaching Brazilian hospital, a retrospective cohort of adult KPC-KP bloodstream infection (BSI) cases (January 2014 to December 2016) was established to study the molecular epidemiology and its impact on outcome (30-day all-cause mortality). KPC-KP isolates underwent multilocus sequence typing. Survival analysis between ST/CC groups and risk factors for fatal outcome (logistic regression) were evaluated. Representative isolates underwent whole-genome sequencing and had their virulence tested in a Galleria larvae model. Results One hundred sixty-five unique KPC-KP BSI cases were identified. CC258 was predominant (66%), followed by ST16 (12%). The overall 30-day mortality rate was 60%; in contrast, 95% of ST16 cases were fatal. Patients’ severity scores were high and baseline clinical variables were not statistically different across STs. In multivariate analysis, ST16 (odds ratio [OR], 21.4; 95% confidence interval [CI], 2.3–202.8; P = .008) and septic shock (OR, 11.9; 95% CI, 4.2–34.1; P < .001) were independent risk factors for fatal outcome. The ST16 clone carried up to 14 resistance genes, including blaKPC-2 in an IncFIBpQIL plasmid, KL51 capsule, and yersiniabactin virulence determinants. The ST16 clone was highly pathogenic in the larvae model. Conclusions Mortality rates were high in this KPC-KP BSI cohort, where CC258 is endemic. An emerging ST16 clone was associated with high mortality. Our results suggest that even in endemic settings, highly virulent clones can rapidly emerge demanding constant monitoring.

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