A Convenient System for Esterification of Fatty Acids

1967 ◽  
Vol 13 (11) ◽  
pp. 1014-1016 ◽  
Author(s):  
Robert L Dryer
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Gas Liquid Chromatography ◽  
Fatty Esters ◽  
Sample Transfer

Abstract A compact apparatus for the methanolysis of lipids and esterification of fatty acids is described. The apparatus is designed to permit the complete preparation of small quantities of fatty esters, without sample transfer, for study by thin-layer or gas-liquid chromatography.

Lipid Composition and Lipid Synthesis in Guinea Pig Megakaryocytes and Platelets

1979 ◽  
Author(s):  
E.P. Schick ◽  
P.K. Schick
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Guinea Pig ◽  
Thin Layer ◽  
Lipid Composition ◽  
Guinea Pigs ◽  
Lipid Synthesis ◽  
Gas Liquid Chromatography ◽  
Lipid Phosphorus ◽  
Layer Chromatography

Lipid composition and lipid synthesis have been compared in guinea pig megakaryocytes and platelets. Megakaryocytes were isolated from guinea pigs to 85% purity. Lipids were extracted and were separated by thin layer chromatography. Phospholipid (PL) content was determined by measurement of lipid phosphorus, and cholesterol and fatty acids were quantitated by gas-liquid chromatography. PL composition of megakaryocytes was:PS + PI 15.2%; SM 14.0%; PC 40.1%; PE 30. 6%. PL composition of platelets was: PS 10.1%; PI 4.5%; SM 16.5%; PC 39.5%; PE 29.6%. The cholesterol:PL ratio was 0.35 for megakaryocytes and 0.55 for platelets. The major fatty acids in the PL were: (% of total)Megakaryocytes and platelets were incubated for 1.5 hr with 14C-acetate. Megakaryocytes incorporated acetate into cholesterol and other sterols, ceramide, and PL (0.060, 0.016 and 0.012 nmoles/105 cells). Platelets incorporated acetate into ceramide and PL (0.02 and 0.06 nmoles/109 cells) but only trace amounts into sterols. There appears to be active biosynthesis of cholesterol in megakaryocytes but not in platelets.

Stereospecific analysis of menhaden oil triacylglycerols and resolution of complex polyunsaturated diacylglycerols by gas–liquid chromatography on polar capillary columns

1990 ◽  
Vol 68 (1) ◽  
pp. 336-344 ◽  
Author(s):  
J. J. Myher ◽  
A. Kuksis ◽  
L.-Y. Yang
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Phospholipase C ◽  
Thin Layer Chromatography ◽  
Molecular Species ◽  
Gas Liquid Chromatography ◽  
Menhaden Oil ◽  
Grignard Degradation ◽  
Layer Chromatography

The sn-1,2-, sn-2,3-, and X-1,3-diacylglycerols derived by Grignard degradation of purified menhaden oil triacylglycerols were isolated by conventional thin-layer chromatography with boric acid complexing. The sn-1,2(2,3)-diacylglycerols were resolved into sn-1,2- and sn-2,3-diacylglycerols by stepwise digestion with phospholipase C of the corresponding phosphatidylcholines and the positional distribution of the fatty acids were determined. Diacylglycerols were converted into trimethylsilyl ethers and resolved on the basis of molecular weight and degree of unsaturation by gas–liquid chromatography using a polar capillary column and isothermal or programmed temperatures. The order of chromatographic elution was established for more than 70 major and minor species by reference to primary and secondary diacylglycerol standards and by calculation of relative retention times. The identified molecular species ranged in carbon number from 28 to 44 and in double bond number from 0 to 12 being made up of C14–C22 fatty acids with 0 to 6 double bonds each and representing the n – 9, n – 6, n – 4, n – 3, and n – 1 series. The gas–liquid chromatographic determinations yielded proportions of all major species that were consistent with those calculated from the knowledge of the stereospecific distribution of the fatty acids in the original triacylglycerol molecules.Key words: Grignard degradation, rac-phosphatidylcholines, phospholipase C, enantiomeric diacylglycerols, thin-layer chromatography, molecular species of diacylglycerols, composition of fatty acids.

AN ANALYSIS OF THE LIPIDS OF GRYLLUS BIMACULATUS (DEGEER) (INSEGTA, ORTHOPTERA)

1967 ◽  
Vol 45 (4) ◽  
pp. 503-505 ◽  
Author(s):  
Paul G. Fast
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Phospholipid Class ◽  
Gas Liquid Chromatography ◽  
Gryllus Bimaculatus ◽  
Acidic Phospholipid ◽  
Choline Phosphoglyceride

The lipids of the cricket Gryllus bimaculatus (DeGeer) have been analyzed by thin-layer and gas–liquid chromatography. Choline phosphoglyceride (54.2%) and ethanolamine phosphoglyceride (35.4%) comprised most of the phospholipids. Smaller amounts of sphingomyelin and an unidentified acidic phospholipid were found. Linoieic acid made up roughly 50% of the fatty acids in each phospholipid class.

The lipid composition of plasma membrane from goat mammary gland

1988 ◽  
Vol 66 (12) ◽  
pp. 1355-1359 ◽  
Author(s):  
Arun Sharma ◽  
Rajvir Dahiya
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Liquid Chromatography ◽  
Mammary Gland ◽  
Thin Layer ◽  
Lipid Composition ◽  
Cholesterol Ester ◽  
Plasma Membranes ◽  
Neutral Lipids ◽  
Gas Liquid Chromatography

Experiments were conducted to examine and characterize the lipid composition of the plasma membrane from the lactating goat mammary gland. The plasma membranes were purified by discontinuous sucrose density centrifugation. Lipids were extracted from these membranes and analyzed by thin-layer and gas–liquid chromatography. The results of these studies demonstrate that (i) the principal phospholipids of mammary-gland plasma membranes are phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin; (ii) the principal neutral lipids are triacylglyceride and cholesterol ester; (iii) the major glycolipids are globotetraosylceramide and globotriaosylceramide; and (iv) the major fatty acids are oleic (18:1), palmitic (16:0), stearic (18:0), and myristic (14:0) acids.

Preliminary studies on the lipid metabolism of Neomysis integer, Involving Labelled Feeding Experiments

1973 ◽  
Vol 53 (3) ◽  
pp. 657-664 ◽  
Author(s):  
R. J. Morris ◽  
C. F. Ferguson ◽  
J. E. G. Raymont
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Lipid Metabolism ◽  
Thin Layer ◽  
Saturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Neomysis Integer ◽  
Feeding Experiments ◽  
Dietary Starch ◽  
Layer Chromatography

A series of controlled, labelled feeding experiments were carried out on Neomysis integer in order to investigate its lipid metabolism. The techniques of thin-layer chromatography, preparative column chromatography, analytical and preparative gas-liquid chromatography and low-background 14C counting were used to follow the incorporation of a 14Clabelled fraction from a range of diets into the animal's lipid. Neomysis integer was found to be capable of converting dietary starch or short-chain saturated fatty acids to longchain polyunsaturated fatty acids, which were then incorporated into the TG and PL fractions.

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

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