Lipid Composition and Lipid Synthesis in Guinea Pig Megakaryocytes and Platelets

1979 ◽  
Author(s):  
E.P. Schick ◽  
P.K. Schick
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Guinea Pig ◽  
Thin Layer ◽  
Lipid Composition ◽  
Guinea Pigs ◽  
Lipid Synthesis ◽  
Gas Liquid Chromatography ◽  
Lipid Phosphorus ◽  
Layer Chromatography

Lipid composition and lipid synthesis have been compared in guinea pig megakaryocytes and platelets. Megakaryocytes were isolated from guinea pigs to 85% purity. Lipids were extracted and were separated by thin layer chromatography. Phospholipid (PL) content was determined by measurement of lipid phosphorus, and cholesterol and fatty acids were quantitated by gas-liquid chromatography. PL composition of megakaryocytes was:PS + PI 15.2%; SM 14.0%; PC 40.1%; PE 30. 6%. PL composition of platelets was: PS 10.1%; PI 4.5%; SM 16.5%; PC 39.5%; PE 29.6%. The cholesterol:PL ratio was 0.35 for megakaryocytes and 0.55 for platelets. The major fatty acids in the PL were: (% of total)Megakaryocytes and platelets were incubated for 1.5 hr with 14C-acetate. Megakaryocytes incorporated acetate into cholesterol and other sterols, ceramide, and PL (0.060, 0.016 and 0.012 nmoles/105 cells). Platelets incorporated acetate into ceramide and PL (0.02 and 0.06 nmoles/109 cells) but only trace amounts into sterols. There appears to be active biosynthesis of cholesterol in megakaryocytes but not in platelets.

Stereospecific analysis of menhaden oil triacylglycerols and resolution of complex polyunsaturated diacylglycerols by gas–liquid chromatography on polar capillary columns

1990 ◽  
Vol 68 (1) ◽  
pp. 336-344 ◽  
Author(s):  
J. J. Myher ◽  
A. Kuksis ◽  
L.-Y. Yang
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Phospholipase C ◽  
Thin Layer Chromatography ◽  
Molecular Species ◽  
Gas Liquid Chromatography ◽  
Menhaden Oil ◽  
Grignard Degradation ◽  
Layer Chromatography

The sn-1,2-, sn-2,3-, and X-1,3-diacylglycerols derived by Grignard degradation of purified menhaden oil triacylglycerols were isolated by conventional thin-layer chromatography with boric acid complexing. The sn-1,2(2,3)-diacylglycerols were resolved into sn-1,2- and sn-2,3-diacylglycerols by stepwise digestion with phospholipase C of the corresponding phosphatidylcholines and the positional distribution of the fatty acids were determined. Diacylglycerols were converted into trimethylsilyl ethers and resolved on the basis of molecular weight and degree of unsaturation by gas–liquid chromatography using a polar capillary column and isothermal or programmed temperatures. The order of chromatographic elution was established for more than 70 major and minor species by reference to primary and secondary diacylglycerol standards and by calculation of relative retention times. The identified molecular species ranged in carbon number from 28 to 44 and in double bond number from 0 to 12 being made up of C14–C22 fatty acids with 0 to 6 double bonds each and representing the n – 9, n – 6, n – 4, n – 3, and n – 1 series. The gas–liquid chromatographic determinations yielded proportions of all major species that were consistent with those calculated from the knowledge of the stereospecific distribution of the fatty acids in the original triacylglycerol molecules.Key words: Grignard degradation, rac-phosphatidylcholines, phospholipase C, enantiomeric diacylglycerols, thin-layer chromatography, molecular species of diacylglycerols, composition of fatty acids.

The lipid composition of plasma membrane from goat mammary gland

1988 ◽  
Vol 66 (12) ◽  
pp. 1355-1359 ◽  
Author(s):  
Arun Sharma ◽  
Rajvir Dahiya
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Liquid Chromatography ◽  
Mammary Gland ◽  
Thin Layer ◽  
Lipid Composition ◽  
Cholesterol Ester ◽  
Plasma Membranes ◽  
Neutral Lipids ◽  
Gas Liquid Chromatography

Experiments were conducted to examine and characterize the lipid composition of the plasma membrane from the lactating goat mammary gland. The plasma membranes were purified by discontinuous sucrose density centrifugation. Lipids were extracted from these membranes and analyzed by thin-layer and gas–liquid chromatography. The results of these studies demonstrate that (i) the principal phospholipids of mammary-gland plasma membranes are phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin; (ii) the principal neutral lipids are triacylglyceride and cholesterol ester; (iii) the major glycolipids are globotetraosylceramide and globotriaosylceramide; and (iv) the major fatty acids are oleic (18:1), palmitic (16:0), stearic (18:0), and myristic (14:0) acids.

LIPID COMPOSITION OF PLATELETS FROM PATIENTS AFFECTED BY Ila HYPERLIPOPROTEINEMIA

1987 ◽  
Author(s):  
D Prisco ◽  
P G Rogasi ◽  
R Paniccia ◽  
A Panetta ◽  
M Coppo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Lipid Composition ◽  
Dietary Habits ◽  
Young Patients ◽  
Thromboxane A2 ◽  
Phospholipid Content ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography

Platelets from patients with familial hypercholesterolemia (IIa HLP), a condition associated with high prevalence of atherosclerosis and of its thrombotic complications, are known to be hyperresponsive to aggregating stimuli and to synthesize increased amounts of thromboxane A2. In order to search if these functional alterations can depend on a different platelet lipid composition, we studied 12 young patients ( aged 20 to 34 years) affected by Ila HLP and 12 suitable controls with similar dietary habits. Lipids were extracted from washed platelets with a chloroform/methanol (2/1) mixture. After silicic acid chromatography and thin-layer chromatography different lipid fractions were eluted and fatty acid methylesters were prepared by acid transmethanolysis. The esters were extracted with hexane and analyzed by gas-liquid chromatography. Different fatty acids were identified on the basis of retention time with respect to standard methylester mixtures and silver nitrate thin-layer chromatography. Cholesterol and lipid phosphorus were assayed by colorimetric methods. Both cholesterol and phospholipid content of platelets were higher in patients than in controls with a significant increase of cholesterol/ phospholipid molar ratio (p<0.05). The percentage content of the phospholipid fractions was not different from that of controls. On the contrary the proportion of saturated fatty acids esterified in the different phospholipid fractions was significantly increased (minimum p<0.05). In addition thromboxane A2 production by platelets from patients with Ila HLP was higher than in controls (<0.001). Our results indicate that lipid composition of platelets from patients with Ila HLP is altered and may be responsible for the enhanced platelet activity described in these patients.

Preliminary studies on the lipid metabolism of Neomysis integer, Involving Labelled Feeding Experiments

1973 ◽  
Vol 53 (3) ◽  
pp. 657-664 ◽  
Author(s):  
R. J. Morris ◽  
C. F. Ferguson ◽  
J. E. G. Raymont
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Lipid Metabolism ◽  
Thin Layer ◽  
Saturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Neomysis Integer ◽  
Feeding Experiments ◽  
Dietary Starch ◽  
Layer Chromatography

A series of controlled, labelled feeding experiments were carried out on Neomysis integer in order to investigate its lipid metabolism. The techniques of thin-layer chromatography, preparative column chromatography, analytical and preparative gas-liquid chromatography and low-background 14C counting were used to follow the incorporation of a 14Clabelled fraction from a range of diets into the animal's lipid. Neomysis integer was found to be capable of converting dietary starch or short-chain saturated fatty acids to longchain polyunsaturated fatty acids, which were then incorporated into the TG and PL fractions.

Diacylglycerol Biosynthesis in Everted Sacs of Rat Intestinal Mucosa

1975 ◽  
Vol 53 (11) ◽  
pp. 1170-1183 ◽  
Author(s):  
W. C. Breckenridge ◽  
A. Kuksis
Keyword(s):  
Fatty Acids ◽  
Phosphatidic Acid ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Thin Layer Chromatography ◽  
Intestinal Mucosa ◽  
Gas Liquid Chromatography ◽  
Acid Pathway ◽  
Layer Chromatography ◽  
Everted Sacs

The molecular specificity in the biosynthesis of diacylglycerols by rat intestinal mucosa was examined by means of radioactive markers, thin-layer chromatography with silver nitrate and gas-liquid chromatography with radioactivity monitoring. Bile salt micelles of alternately labeled monoacylglycerols and free fatty acids were incubated with everted sacs of intestinal mucosa for various periods of time and the diacylglycerols were isolated by solvent extraction and thin-layer chromatography. Stereospecific analyses of the X-1,2-diacylglycerols labeled from 2-monoacylgiycerols showed that the sn-1,2-isomers (45–55%) were slightly in excess of the sn-2,3-isomers (34–45%) with the X-1,3-diacylglycerols accounting for the rest of the radioactivity (5–10%). This suggests that racemic diacylglycerols may be intermediates in the resynthesis of dietary fat in rat intestinal mucosa. Detailed analyses of the molecular species of the sn-1,2-diacylglycerols labeled from free fatty acids revealed that 10–45% of the total did not contain the acid present in the 2-monoacylglycerol supplied, and therefore had originated from the phosphatidic acid pathway. These findings are at variance with those obtained in isolated microsomes, which have suggested an inhibition of the phosphatidic acid pathway by monoacylglycerols as well as have given evidence of an exclusive synthesis of sn-1,2-diacylglycerols from 2-monoacylglycerols.

ANALYSIS OF CHLORORGANIC COMPOUNDS IN FODDER FOR BROILERCHICKENS BY THE METHOD OF GAS-LIQUID CHROMATOGRAPHY WITH MASSSPECTROMETER DETECTOR

2020 ◽  
Vol 1 (2) ◽  
pp. 205-208
Author(s):  
A.A. Parshutina ◽  
◽  
A.A. Solovyova ◽  
L.P. Satyukova ◽  
E.G. Shubina ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Organochlorine Pesticides ◽  
Mass Spectrometer ◽  
Broiler Chickens ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography

The article shows the importance of the study of feed for broiler chickens on the content of organochlorine pesticides. These substances in significant concentrations can disrupt the development of birds and cause mass poisoning. The study of formula feed for broiler chickens «prestart» and «start» by two methodswas conducted: a certified method for the determination of organochlorine pesticides in feed and formula feed (thin layer chromatography) and a method for detecting pesticides not certified for feed (gas-liquid chromatography with mass spectrometer detector). During the experiment, the presence of organochlorine pesticides in several formulafeed samples was revealed.

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