The lipid composition of plasma membrane from goat mammary gland

1988 ◽  
Vol 66 (12) ◽  
pp. 1355-1359 ◽  
Author(s):  
Arun Sharma ◽  
Rajvir Dahiya
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Liquid Chromatography ◽  
Mammary Gland ◽  
Thin Layer ◽  
Lipid Composition ◽  
Cholesterol Ester ◽  
Plasma Membranes ◽  
Neutral Lipids ◽  
Gas Liquid Chromatography

Experiments were conducted to examine and characterize the lipid composition of the plasma membrane from the lactating goat mammary gland. The plasma membranes were purified by discontinuous sucrose density centrifugation. Lipids were extracted from these membranes and analyzed by thin-layer and gas–liquid chromatography. The results of these studies demonstrate that (i) the principal phospholipids of mammary-gland plasma membranes are phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin; (ii) the principal neutral lipids are triacylglyceride and cholesterol ester; (iii) the major glycolipids are globotetraosylceramide and globotriaosylceramide; and (iv) the major fatty acids are oleic (18:1), palmitic (16:0), stearic (18:0), and myristic (14:0) acids.

Lipid Composition and Lipid Synthesis in Guinea Pig Megakaryocytes and Platelets

1979 ◽  
Author(s):  
E.P. Schick ◽  
P.K. Schick
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Guinea Pig ◽  
Thin Layer ◽  
Lipid Composition ◽  
Guinea Pigs ◽  
Lipid Synthesis ◽  
Gas Liquid Chromatography ◽  
Lipid Phosphorus ◽  
Layer Chromatography

Lipid composition and lipid synthesis have been compared in guinea pig megakaryocytes and platelets. Megakaryocytes were isolated from guinea pigs to 85% purity. Lipids were extracted and were separated by thin layer chromatography. Phospholipid (PL) content was determined by measurement of lipid phosphorus, and cholesterol and fatty acids were quantitated by gas-liquid chromatography. PL composition of megakaryocytes was:PS + PI 15.2%; SM 14.0%; PC 40.1%; PE 30. 6%. PL composition of platelets was: PS 10.1%; PI 4.5%; SM 16.5%; PC 39.5%; PE 29.6%. The cholesterol:PL ratio was 0.35 for megakaryocytes and 0.55 for platelets. The major fatty acids in the PL were: (% of total)Megakaryocytes and platelets were incubated for 1.5 hr with 14C-acetate. Megakaryocytes incorporated acetate into cholesterol and other sterols, ceramide, and PL (0.060, 0.016 and 0.012 nmoles/105 cells). Platelets incorporated acetate into ceramide and PL (0.02 and 0.06 nmoles/109 cells) but only trace amounts into sterols. There appears to be active biosynthesis of cholesterol in megakaryocytes but not in platelets.

A Convenient System for Esterification of Fatty Acids

1967 ◽  
Vol 13 (11) ◽  
pp. 1014-1016 ◽  
Author(s):  
Robert L Dryer
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Gas Liquid Chromatography ◽  
Fatty Esters ◽  
Sample Transfer

Abstract A compact apparatus for the methanolysis of lipids and esterification of fatty acids is described. The apparatus is designed to permit the complete preparation of small quantities of fatty esters, without sample transfer, for study by thin-layer or gas-liquid chromatography.

Changes in lipid composition of oat root membranes as a function of water-deficit stress

1985 ◽  
Vol 63 (2) ◽  
pp. 77-84 ◽  
Author(s):  
C. Liljenberg ◽  
M. Kates
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Water Deficit ◽  
Lipid Composition ◽  
Membrane Fraction ◽  
Plasma Membranes ◽  
Root Tip ◽  
Water Deficit Stress ◽  
Root Cells ◽  
Steryl Esters

The effect of repeated water-deficit stress on the lipid composition of root cells from 5-day-old oat (Avena sativa L. cv. Seger) seedlings was studied. The content of total acyl lipids was found to decrease with increasing degree of water-deficit stress, owing largely to decreases in free fatty acids, triglycerides, phosphatidylethanolamine (PE), wax esters, steryl esters, and acylated steryl glycosides. Major polar lipids both in total root cells and in the plasma membrane enriched fraction, as well as the microsomal membrane fraction, were PE, phosphatidylcholine (PC), digalactosyldiacylglycerol (DGDG), monogalactosyldiacylglycerol (MGDG), and polyglycolipid. Decreases in the degree of unsaturation of the fatty acids as a funtion of increased water-deficit stress were observed for the MGDG and polyglycolipid components of total root cells and for the MGDG, DGDG, and polyglycolipid of the plasma membrane fraction. Electron microscopy showed that stressed root tip cells had much smoother plasma membranes than those of control unstressed root cells. These results suggest that root cells of oat seedlings respond to water-deficit stress by reducing the total plasma membrane mass and degree of lipid fluidity, which would reduce the water permeability of the plasma membranes and help maintain cell turgidity.

scholarly journals STUDY OF THE LIPID COMPOSITION OF SOME PLANTS GROWING IN GEORGIA

2019 ◽  
pp. 24-25
Author(s):  
B. Kikalishvili ◽  
Ts. Sulakvelidze ◽  
M. Malania ◽  
D. Turabelidze
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitis Vinifera ◽  
Lipid Composition ◽  
High Performance ◽  
Neutral Lipids ◽  
Physical Chemical ◽  
Neutral And Polar Lipids

Sums of neutral and polar lipids were extracted from the fruits of Vitis vinifera and pericarpium of Physalis alkekengi L.; their main classes and physical-chemical constants of some neutral lipids were determined. The saturated, unsaturated and polyunsaturated fatty acids were identified by using High Performance Liquid Chromatography. The phospholipids- lysophosphatidylcholine, phosphatidylinosite, phosphatidylcholine, phosphatidylethanolamine and N-acyl phosphatidylethanolamine were identified in them. The content of carotenoids is up 2,5 mg% and 65,5mg% in Vitis vinifera and Physalis alkekengi L. respectively. Amino acids were detected in studied species. The oils from both plants are characterized with anti-inflammatory, hepatoprotective, choleretic and immunotropic activities, they can be used in medicine for curative and preventive purposes.

Stereospecific analysis of menhaden oil triacylglycerols and resolution of complex polyunsaturated diacylglycerols by gas–liquid chromatography on polar capillary columns

1990 ◽  
Vol 68 (1) ◽  
pp. 336-344 ◽  
Author(s):  
J. J. Myher ◽  
A. Kuksis ◽  
L.-Y. Yang
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Phospholipase C ◽  
Thin Layer Chromatography ◽  
Molecular Species ◽  
Gas Liquid Chromatography ◽  
Menhaden Oil ◽  
Grignard Degradation ◽  
Layer Chromatography

The sn-1,2-, sn-2,3-, and X-1,3-diacylglycerols derived by Grignard degradation of purified menhaden oil triacylglycerols were isolated by conventional thin-layer chromatography with boric acid complexing. The sn-1,2(2,3)-diacylglycerols were resolved into sn-1,2- and sn-2,3-diacylglycerols by stepwise digestion with phospholipase C of the corresponding phosphatidylcholines and the positional distribution of the fatty acids were determined. Diacylglycerols were converted into trimethylsilyl ethers and resolved on the basis of molecular weight and degree of unsaturation by gas–liquid chromatography using a polar capillary column and isothermal or programmed temperatures. The order of chromatographic elution was established for more than 70 major and minor species by reference to primary and secondary diacylglycerol standards and by calculation of relative retention times. The identified molecular species ranged in carbon number from 28 to 44 and in double bond number from 0 to 12 being made up of C14–C22 fatty acids with 0 to 6 double bonds each and representing the n – 9, n – 6, n – 4, n – 3, and n – 1 series. The gas–liquid chromatographic determinations yielded proportions of all major species that were consistent with those calculated from the knowledge of the stereospecific distribution of the fatty acids in the original triacylglycerol molecules.Key words: Grignard degradation, rac-phosphatidylcholines, phospholipase C, enantiomeric diacylglycerols, thin-layer chromatography, molecular species of diacylglycerols, composition of fatty acids.

AN ANALYSIS OF THE LIPIDS OF GRYLLUS BIMACULATUS (DEGEER) (INSEGTA, ORTHOPTERA)

1967 ◽  
Vol 45 (4) ◽  
pp. 503-505 ◽  
Author(s):  
Paul G. Fast
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Phospholipid Class ◽  
Gas Liquid Chromatography ◽  
Gryllus Bimaculatus ◽  
Acidic Phospholipid ◽  
Choline Phosphoglyceride

The lipids of the cricket Gryllus bimaculatus (DeGeer) have been analyzed by thin-layer and gas–liquid chromatography. Choline phosphoglyceride (54.2%) and ethanolamine phosphoglyceride (35.4%) comprised most of the phospholipids. Smaller amounts of sphingomyelin and an unidentified acidic phospholipid were found. Linoieic acid made up roughly 50% of the fatty acids in each phospholipid class.

Mechanism of hypoxic injury to pulmonary artery endothelial cell plasma membranes

1989 ◽  
Vol 257 (2) ◽  
pp. C223-C231 ◽  
Author(s):  
E. R. Block ◽  
J. M. Patel ◽  
D. Edwards
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Endothelial Cell ◽  
Pulmonary Artery ◽  
Lipid Composition ◽  
Plasma Membranes ◽  
Membrane Lipid ◽  
Conjugated Dienes ◽  
Membrane Phospholipids ◽  
Cell Plasma

We exposed monolayer cultures of pulmonary artery endothelial cells or plasma membranes derived from these cells to hypoxic (0 and 5% O2) and normoxic (20% O2; control) conditions and measured cellular contents of malondialdehyde and conjugated dienes, plasma membrane fluidity and lipid composition, and plasma membrane-dependent transport of 5-hydroxytryptamine (5-HT). Hypoxia caused significant increases in malondialdehyde and conjugated dienes, in fluidity, and in 5-HT transport. Hypoxia also caused a significant decrease in plasma membrane total phospholipids and a marked increase in plasma membrane free fatty acids that appeared to be due to release of fatty acids from the plasma membrane phospholipids. The increases in fluidity and 5-HT transport and the alterations in fatty acids were reversible after return to control conditions. These results indicate that hypoxia alters the physical state, lipid composition, and function of endothelial cell plasma membranes by a combination of stimulation of membrane lipid peroxidation and accelerated degradation of membrane phospholipids, the latter probably secondary to activation of membrane phospholipases.

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