Semimicro Method for Determination of Serum Uric Acid Using EDTA—Hydrazine

1968 ◽  
Vol 14 (8) ◽  
pp. 764-775 ◽  
Author(s):  
C P Patel
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Serum Uric Acid ◽  
Phosphotungstic Acid ◽  
Sodium Salicylate ◽  
Sodium Tungstate ◽  
Hydrazine Sulfate ◽  
Spectrophotometric Methods ◽  
Automated Procedures

Abstract A semimicro method is described for the determination of uric acid by manual and automated procedures. Serum uric acid reduces phosphotungstic acid in an alkaline medium of EDTA sodium tungstate and hydrazine sulfate, the latter being used as a color intensifier. The use of sodium tungstate eliminates the development of interfering turbidity encountered with other alkalizing reagents. This method requires only 0.2 ml. of serum. The presence of ascorbic acid, sodium salicylate, cysteine, and/or glucose does not interfere. A linear relationship is obtained upon comparing this method with the sodium carbonate and the differential ultraviolet spectrophotometric methods.

Non-Urate Chromogens in Body Fluids

1969 ◽  
Vol 15 (8) ◽  
pp. 720-726 ◽  
Author(s):  
Wendell T Caraway
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Phosphotungstic Acid ◽  
Alkaline Treatment ◽  
Body Fluids ◽  
Plasma Ascorbic Acid ◽  
A Value ◽  
Fresh Plasma ◽  
Average Serum

Abstract Compounds which interfere in the analytic determination of uric acid are referred to as "non-urate chromogens." Ascorbic acid, when added to serum, causes a significant increase in apparent uric acid levels as determined by a carbonate-phosphotungstate method. The ratio of apparent urate to ascorbic acid is 1:3, a value in close agreement with that obtained for the ratio of labile non-urate chromogens to ascorbic acid in fresh plasma. Ascorbic acid, as well as most of the non-urate chromogens, can be eliminated by mild alkaline treatment prior to adding phosphotungstic acid. These results indicate that the major non-urate chromogen in the average serum is ascorbic acid. The nature of other non-urate chromogens in body fluids is reviewed.

Simplified Automated Method for Determination of Urinary or Serum Uric Acid, Based on Reduction of Ferric-Phenanthroline Complex

1975 ◽  
Vol 21 (1) ◽  
pp. 125-129 ◽  
Author(s):  
John W Nelson ◽  
K K Batra
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Reduction Method ◽  
Phosphotungstic Acid ◽  
Colorimetric Method ◽  
Automated Method ◽  
Phenanthroline Complex ◽  
Acidic Conditions ◽  
Acid Reduction

Abstract We describe an automated colorimetric method for determination of uric acid in serum or urine by use of an AutoAnalyzer II or SMA 12/60 (Technicon Corp.). The method depends on reduction of a ferric-phenanthroline complex by uric acid under acidic conditions to a ferrous-phenanthroline complex, which absorbs at 505 nm. Advantages of this method over other methods now in use are that color and concentration are linearly related (to 20 mg/100 ml); aqueous reagents are easily prepared, stable, and inexpensive; and interference from ascorbic acid has been eliminated by use of an alkaline copper-containing diluent. The analysis is also free of interference from glucose, creatinine, glutathione, salicylates and hemoglobin. Correlation with results of the Technicon phosphotungstic acid reduction method is excellent (r = .996). Correlation with results of an automated uricase method is satisfactory (r = .979). Recovery of uric acid was 101% over a wide concentration range.

scholarly journals Evaluation of the Enzymatic Assay of Serum Uric Acid with 2,2′-Azino-Di(3-Ethylbenzthiazoline-6-Sulphonate) (ABTS) as Chromogen

1984 ◽  
Vol 21 (6) ◽  
pp. 504-509 ◽  
Author(s):  
Nada Majkić-Singh ◽  
Bouz Asal Said ◽  
Slavica Spasić ◽  
Ivan Berkeš
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Serum Uric Acid ◽  
Reference Values ◽  
Spectrophotometric Method ◽  
Enzymatic Assay ◽  
Standard Curve ◽  
Male Subjects ◽  
Enzymatic Methods

A spectrophotometric method for the determination of serum uric acid based on the oxidation of 2,2′-azino-di(3-ethylbenzthiazoline-6-sulphonate) by use of uricase and peroxidase has already been reported. The method is very precise (CV <4·7%). The standard curve is linear up to 4640 μmol/L. Comparison with other enzymatic methods gave good correlation. The method gave results 9% lower than the phosphotungstate method. The effects of bilirubin, haemoglobin, glucose, ascorbic acid, anticoagulants and purine compounds were studied. The reference values for this method are 140·8–407·8 μmol/L for female subjects and 145·6–514·7 μmol/L for male subjects.

Ultramicro Automated Screening Method for Uric Acid

1967 ◽  
Vol 13 (11) ◽  
pp. 985-993 ◽  
Author(s):  
Ronald H Laessig ◽  
Chester E Underwood ◽  
Barbara J Basteyns
Keyword(s):  
Uric Acid ◽  
Phosphotungstic Acid ◽  
Screening Method ◽  
Sampling Procedure ◽  
Blood Capillary ◽  
Acid Complex ◽  
Blood Uric Acid ◽  
Precision And Accuracy ◽  
Automated Screening

Abstract An automated colorimetric microprocedure, suitable for screening purposes, has been developed for the determination of blood uric acid levels. The method uses 2O-µl. whole-blood (capillary) samples and is based on the AutoAnalyzer measurement of the absorbance of the colored uric acid-phosphotungstic acid complex. The dilution inherent in the sampling procedure necessitated a modification of the existing AutoAnalyzer method to increase the sensitivity. The proposed method is evaluated for precision and accuracy by comparison with the standard AutoAnalyzer macro-method.

3DGH-Fc based electrochemical sensor for the simultaneous determination of ascorbic acid, dopamine and uric acid

2017 ◽  
Vol 799 ◽  
pp. 459-467 ◽  
Author(s):  
Qing Zhu ◽  
Jing Bao ◽  
Danqun Huo ◽  
Mei Yang ◽  
Huixiang Wu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Electrochemical Sensor ◽  
Simultaneous Determination

The Determination of Catechols in the Presence of Ascorbic Acid and Uric Acid by Flow Injection Analysis Employing a Potentiometric Dibenzo-18-crown-6 Electrode Detector

1994 ◽  
Vol 27 (11) ◽  
pp. 2141-2151 ◽  
Author(s):  
Suzanne K. Lunsford ◽  
A. Galal ◽  
N. Akmal ◽  
Y. L. Ma ◽  
H. Zimmer ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Flow Injection ◽  
Flow Injection Analysis
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