scholarly journals Evaluation of the Enzymatic Assay of Serum Uric Acid with 2,2′-Azino-Di(3-Ethylbenzthiazoline-6-Sulphonate) (ABTS) as Chromogen

1984 ◽  
Vol 21 (6) ◽  
pp. 504-509 ◽  
Author(s):  
Nada Majkić-Singh ◽  
Bouz Asal Said ◽  
Slavica Spasić ◽  
Ivan Berkeš
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Serum Uric Acid ◽  
Reference Values ◽  
Spectrophotometric Method ◽  
Enzymatic Assay ◽  
Standard Curve ◽  
Male Subjects ◽  
Enzymatic Methods

A spectrophotometric method for the determination of serum uric acid based on the oxidation of 2,2′-azino-di(3-ethylbenzthiazoline-6-sulphonate) by use of uricase and peroxidase has already been reported. The method is very precise (CV <4·7%). The standard curve is linear up to 4640 μmol/L. Comparison with other enzymatic methods gave good correlation. The method gave results 9% lower than the phosphotungstate method. The effects of bilirubin, haemoglobin, glucose, ascorbic acid, anticoagulants and purine compounds were studied. The reference values for this method are 140·8–407·8 μmol/L for female subjects and 145·6–514·7 μmol/L for male subjects.

Semimicro Method for Determination of Serum Uric Acid Using EDTA—Hydrazine

1968 ◽  
Vol 14 (8) ◽  
pp. 764-775 ◽  
Author(s):  
C P Patel
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Serum Uric Acid ◽  
Phosphotungstic Acid ◽  
Sodium Salicylate ◽  
Sodium Tungstate ◽  
Hydrazine Sulfate ◽  
Spectrophotometric Methods ◽  
Automated Procedures

Abstract A semimicro method is described for the determination of uric acid by manual and automated procedures. Serum uric acid reduces phosphotungstic acid in an alkaline medium of EDTA sodium tungstate and hydrazine sulfate, the latter being used as a color intensifier. The use of sodium tungstate eliminates the development of interfering turbidity encountered with other alkalizing reagents. This method requires only 0.2 ml. of serum. The presence of ascorbic acid, sodium salicylate, cysteine, and/or glucose does not interfere. A linear relationship is obtained upon comparing this method with the sodium carbonate and the differential ultraviolet spectrophotometric methods.

Chemometric-assisted kinetic–spectrophotometric method for simultaneous determination of ascorbic acid, uric acid, and dopamine

2011 ◽  
Vol 410 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Masoud Rohani Moghadam ◽  
Shayessteh Dadfarnia ◽  
Ali Mohammad Haji Shabani ◽  
Parviz Shahbazikhah
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Kinetic Spectrophotometric

The 4-hydroxybenzoate/4-aminophenazone chromogenic system used in the enzymic determination of serum cholesterol.

1978 ◽  
Vol 24 (12) ◽  
pp. 2161-2165 ◽  
Author(s):  
F Meiattini ◽  
L Prencipe ◽  
F Bardelli ◽  
G Giannini ◽  
P Tarli
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Linear Regression ◽  
Serum Cholesterol ◽  
Reduced Glutathione ◽  
Cholesterol Oxidase ◽  
Dihydroxybenzoic Acid ◽  
Standard Curve ◽  
Analytical Recovery

Abstract A single reagent, containing cholesterol oxidase, cholesterol esterase, peroxidase, 4-hydroxybenzoate, and 4-aminophenazone, is used in determining serum cholesterol. Analysis time is 15 min, and the standard curve is linear to 6.0 g/liter. Analytical recovery of cholesterol was 100.1 +/- 0.4%. Within-run precision (CV) was less than or equal to 1.4 1.4%, between-run less than or equal to 4.8%. Comparison with results by a Liebermann Burchard method [Clin. Chim. Acta 5, 637 (1960)] gave a linear regression of y = 1.08x--0.05, with a correlation coefficient (r) of 0.985. Comparison with the Roeschlau enzymic method [J. Clin. Chem. Clin. Biochem, 12, 226 (1974)] gave y = 1.02x + 0.01 (r = 0.958). Comparison with the enzymic method of Allain et al. [Clin. Chem. 20, 470 (1974)] gave y = 1.01x--0.00 (r = 0.995). The following substances do not interfere up to the indicated concentrations (mg/liter): hemoglobin (5000), bilirubin (100), reduced glutathione (150), l-cysteine (400), urea (3000), creatinine (200), uric acid (200), d-glucose (10000), L-ascorbic acid (50), acetylsalicylic acid (500), L-DOPA (10), ergothioneine (1000), 2,5-dihydroxybenzoic acid (20), and 3,4-dihydroxybenzoic acid (10). Stored in an amber-colored bottle, the working reagent is stable for three months at 2--8 degrees C and for three weeks at 25 degrees C.

3DGH-Fc based electrochemical sensor for the simultaneous determination of ascorbic acid, dopamine and uric acid

2017 ◽  
Vol 799 ◽  
pp. 459-467 ◽  
Author(s):  
Qing Zhu ◽  
Jing Bao ◽  
Danqun Huo ◽  
Mei Yang ◽  
Huixiang Wu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Electrochemical Sensor ◽  
Simultaneous Determination

The Determination of Catechols in the Presence of Ascorbic Acid and Uric Acid by Flow Injection Analysis Employing a Potentiometric Dibenzo-18-crown-6 Electrode Detector

1994 ◽  
Vol 27 (11) ◽  
pp. 2141-2151 ◽  
Author(s):  
Suzanne K. Lunsford ◽  
A. Galal ◽  
N. Akmal ◽  
Y. L. Ma ◽  
H. Zimmer ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Flow Injection ◽  
Flow Injection Analysis

scholarly journals Spectrophotometric determination of phosphorus in coal and coal ash using bismuth-phosphomobybdate complex

2003 ◽  
Vol 68 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Randjel Mihajlovic ◽  
Natasa Ignjatovic ◽  
Marija Todorovic ◽  
Ivanka Holclajtner-Antunovic ◽  
Vesna Kaljevic
Keyword(s):  
Aqueous Solution ◽  
Ascorbic Acid ◽  
Acid Medium ◽  
Spectrophotometric Method ◽  
Methyl Ketone ◽  
Coal Ash ◽  
Molar Absorptivity ◽  
Stable Complex ◽  
Tolerance Limits

A modified spectrophotometric method using the bismuth phosphomolybdate complex for the determination of phosphorus in coal and coal ash is suggested. Bismuth together with phosphate and molybdate forms a very stable complex in acid medium which turns blue ("molibdenum blue") by reduction with ascorbic acid. The apparent molar absorptivity of PBiMo is 1.66x104 dm3 mol-1cm-1 at 720 nm and 2.10x104 dm3 mol-1cm-1 at 670 nm isobutyl methyl ketone (MIBK). Interference caused by the ions present are within the tolerance limits (?2 %). Beer?s law is obeyed in the for concentration range to 0.6 ?g/mL (aqueous solution) and to 1.2 ?g/mL P (MIBK). The sensitivity of the proposed method is 0.0078 ?g/mL (aqueous solution) and 0.0066 ?g/mL (MIBK).

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