Automated Elution Electrophoresis: A Potential Clinical Tool
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Abstract An elution electrophoresis system in which a porous packed bed is used for separation and a flow photometer or colorimeter for continuous monitoring of the eluate may be capable of rapid, high-resolution analysis of serum proteins and other protein mixtures with very little manual labor. In a prototype of such a system we used a cooled separations column, 3 mm in diameter and 40 cm long, containing polyacrylamide beads. Samples are introduced, via a microsyringe, through a septum at the column midpoint. Typical analyses for serum proteins or serum isoenzymes require an electrophoresis time of about 30 min at 1200 V.
1994 ◽
Vol 144
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pp. 593-596
2020 ◽
Vol 14
(S1)
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pp. S146-S150
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2002 ◽
Vol 70
(5)
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pp. 1197-1214
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2006 ◽
Vol 23
(11)
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pp. 2101-2111
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