Immobilized glucose oxidase used in the continuous-flow determination of serum glucose.

1976 ◽  
Vol 22 (7) ◽  
pp. 1017-1023 ◽  
Author(s):  
L P León ◽  
S Narayanan ◽  
R Dellenbach ◽  
C Horvath
Keyword(s):  
Glucose Oxidase ◽  
Continuous Flow ◽  
Serum Glucose ◽  
Long Term Storage ◽  
Free Glucose ◽  
Immobilized Glucose Oxidase ◽  
Analytical System ◽  
Single Enzyme

Abstract We used a tubular glucose-oxidase wall reactor in the "AutoAnalyzer II" continuous-flow analytical system to determine glucose in blood serum. Sensitivity was high and wash characteristics were satisfactory with use of a 30-cm tube containing immobilized glucose oxidase. Results compared favorably with those of the conventional free-enzyme method. More than 25000 such assays can be performed with a single enzyme tube, which also shows long-term storage stability. Because of the steady-state chemistry 60 samples can be analyzed per hour. The linearity of the method is excellent and sample interaction from 5.0 to 1.0 g/liter is less than 5%. Results correlate well (greater than 0.993) with those obtained with both the neocuproine method used in the "SMA 12/60" multichannel analytical system and the free glucose oxidase method used in the AutoAnalyzer.

Preparation And elemental analysis of ancient fibers

1987 ◽  
Vol 45 ◽  
pp. 410-411
Author(s):  
Allen Angel ◽  
Kathryn A. Jakes
Keyword(s):  
Cross Sections ◽  
Physical Structure ◽  
Energy Dispersive ◽  
Archaeological Sites ◽  
X Ray ◽  
Long Term Storage ◽  
Backscattered Electron ◽  
Electron Imaging

Fabrics recovered from archaeological sites often are so badly degraded that fiber identification based on physical morphology is difficult. Although diagenetic changes may be viewed as destructive to factors necessary for the discernment of fiber information, changes occurring during any stage of a fiber's lifetime leave a record within the fiber's chemical and physical structure. These alterations may offer valuable clues to understanding the conditions of the fiber's growth, fiber preparation and fabric processing technology and conditions of burial or long term storage (1).Energy dispersive spectrometry has been reported to be suitable for determination of mordant treatment on historic fibers (2,3) and has been used to characterize metal wrapping of combination yarns (4,5). In this study, a technique is developed which provides fractured cross sections of fibers for x-ray analysis and elemental mapping. In addition, backscattered electron imaging (BSI) and energy dispersive x-ray microanalysis (EDS) are utilized to correlate elements to their distribution in fibers.

scholarly journals Determination of standards of raw timber natural losses due to shrinkage at long-term dry storage

2021 ◽  
Vol 67 (No. 5) ◽  
pp. 195-203
Author(s):  
Dalibor Šafařík ◽  
Petra Hlaváčková ◽  
David Březina
Keyword(s):  
Woody Plants ◽  
Mean Value ◽  
The Czech Republic ◽  
Long Term Storage ◽  
Upper Limits ◽  
Natural Losses ◽  
Term Storage

The aim of the article is to describe the issue of determining the characteristics and parameters of raw timber natural losses due to shrinkage at long-term storage, defining the theoretical basis for creating standards, and verify its finding by means of a case study in raw timber storing. This issue is very topical in forestry practice in the Czech Republic as well as in other countries. The lower and upper limits of the standards were calculated, and the proposed mean value was grouped according to woody plants that reflected the most frequent commercial usage with respect to subsequent processing. Subsequently, experimental verification was carried out on a representative sample of 2 209.99 m3 of raw timber of Norway spruce (Picea abies /L./ H. Karst.) at selected forest administrations. Furthermore, the article addresses the related accounting and tax issues of the standards of natural losses of raw timber. Currently, no legislation mandates an entity to establish standards.

Enzyme thermistor determination of glucose in serum using immobilized glucose oxidase

1977 ◽  
Vol 81 (2) ◽  
pp. 163-175 ◽  
Author(s):  
B. Danielsson ◽  
Kerstin Gadd ◽  
B. Mattiasson ◽  
K. Mosbach
Keyword(s):  
Glucose Oxidase ◽  
Immobilized Glucose Oxidase ◽  
Enzyme Thermistor

Retrospective statistical analysis of lyophilized protein formulations of progenipoietin using PLS: Determination of the critical parameters for long-term storage stability

2004 ◽  
Vol 93 (10) ◽  
pp. 2609-2623 ◽  
Author(s):  
Derrick S. Katayama ◽  
Carol F. Kirchhoff ◽  
Carrie M. Elliott ◽  
Robert E. Johnson ◽  
Jeffry Borgmeyer ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Storage Stability ◽  
Critical Parameters ◽  
Long Term Storage ◽  
Protein Formulations ◽  
Term Storage

An Automated, Saccharogenic Method for Determining Serum Amylase Activity

1970 ◽  
Vol 16 (12) ◽  
pp. 985-989 ◽  
Author(s):  
Wendell R O'Neal ◽  
Nathan Gochman
Keyword(s):  
Glucose Oxidase ◽  
Blood Donors ◽  
Serum Amylase ◽  
Amylase Activity ◽  
Serum Glucose ◽  
Normal Range ◽  
Reducing Substances ◽  
Serum Amylase Activity

Abstract An automated adaptation of the Somogyi saccharogenic determination of serum amylase is described in which conventional AutoAnalyzer modules are used. Adequate sensitivity with short incubation is achieved by incorporating glucose oxidase and catalase in the substrate to destroy serum glucose during incubation. Maltose and other dialyzable oligosaccharides are measured with the alkaline copper-neocuproine reaction. A simultaneous blank run is performed to determine reducing substances other than glucose in serum. Precision studies and correlation with a manual saccharogenic method are presented. The normal range was determined from data for 49 healthy blood donors.

Application of Fe(II)-5-Pyridyl Benzodiazepin-2-ones to the Determination of Plasma or Serum Glucose

1971 ◽  
Vol 17 (12) ◽  
pp. 1196-1199
Author(s):  
Bernard Klein ◽  
Lois B Lucas
Keyword(s):  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Serum Glucose ◽  
Present Technique ◽  
The Mean

Abstract A new coupled redox procedure has been developed for the determination of glucose in 4 µl of plasma or serum. The photometric endpoint is formation of the violet-blue Fe2+-5-pyridyl benzodiazepin-2-one chelate, for which the absorbance at 580 nm is linear with glucose concentration from 25 to 300 mg/100 ml. Results obtained by the present technique and those obtained by the o-toluidine method and a glucose oxidase method agree well. The mean recovery of glucose added to plasma is 96.6% (range: 92.1-99.6%).

scholarly journals Moulds occurrence in woodchips

2014 ◽  
Vol 60 (No. 4) ◽  
pp. 155-158
Author(s):  
J. Souček
Keyword(s):  
Water Content ◽  
Wood Chips ◽  
Effect Of Water ◽  
Long Term Storage ◽  
Term Storage ◽  
Storage Temperatures

The research, whose results are presented, is aimed at determination of development of moulds number in wood chips under different storage temperatures. The experiments were carried out with the moisture of samples 65%, 22% and 1%. During the long-term storage the effect of water content in material on development of moulds can be recorded. The risks linked to mould occurrence can be considerably eliminated by reduction of water content.  

Sign in / Sign up

Export Citation Format

Share Document