Analysis for glutathione in blood by high-performance liquid chromatography.

1978 ◽  
Vol 24 (7) ◽  
pp. 1140-1143 ◽  
Author(s):  
D L Rabenstein ◽  
R Saetre
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Colorimetric Assay ◽  
High Performance Liquid Chromatographic ◽  
Electrochemical Detector ◽  
Nitrobenzoic Acid ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract A high-performance liquid chromatographic method is presented for determination of glutathione in whole blood. Sample preparation involves hemolysis, protein precipitation, centrifugation, and filtration. The glutathione in the filtrate is then separated from other sulfhydryl-containing molecules by liquid chromatography with Zipax SCX cation-exchanger followed by detection with a mercury-based electrochemical detector. The liquid-chromatographic analysis time is approximately 5 min. Because of the chromatographic separation and the selectivity of the detector, the detection step is free from interferences from other components of blood. The method has been checked by comparison with the colorimetric assay based on reaction with 5,5'-dithiobis(2-nitrobenzoic acid). The chromatographic results are consistently slightly lower, presumably because of the greater selectivity of this method.

scholarly journals The high-performance liquid-chromatographic analysis of ficaprenol and dolichol

1977 ◽  
Vol 165 (2) ◽  
pp. 405-408 ◽  
Author(s):  
R W Keenan ◽  
N Rice ◽  
R Quock
Keyword(s):  
Chromatographic Analysis ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Pig Liver ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Derivatives Of

A high-performance liquid-chromatographic method was devised which is capable of resolving the p-nitrobenzoyl derivatives of polyprenols containing 35-110 or more carbon atoms. This procedure was used for the determination of ficaprenol and pig liver dolichol composition and can be applied to mixtures of polyprenols as an analytical or preparative technique.

scholarly journals High-performance liquid chromatography of phosphatidylcholine and sphingomyelin with detection in the region of 200 nm

1976 ◽  
Vol 155 (1) ◽  
pp. 55-60 ◽  
Author(s):  
F B Jungalwala ◽  
J E Evans ◽  
R H McCluer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Animal Tissues ◽  
Degree Of Unsaturation ◽  
Liquid Chromatographic Method ◽  
Absorbing Material ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

A sensitive method for the separation of phosphatidylcholine and sphingomyelin by high-performance liquid chromatographic analysis is described. The elution of the phospholipids from a microparticulate (10 mum) silica-gel chromatographic column was monitored with an ultraviolet spectromonitor at 203 nm. Acetonitrile/methanol/water (65:21:14, by vol.) was used as the solvent. It was shown by using synthetic phosphatidylcholines of knowm fatty acid composition and of varying degree of unsaturation that the absorption at 203 nm was primarily due to the isolated double bonds and the response measured varied with the degree of unsaturation. Approx. 1 nmol of phosphatidylcholine, containing at least one double bond per molecule, can be detected. The amounts of phosphatidylcholine and sphingomyelin could be determined by high-performance liquid chromatography and ultraviolet absorption if the apparent extinction coefficient of the material analyzed was established. Alternatively, peaks were collected and the phospholipids were determined by the analysis of phosphorus. The analysis of phosphatidylcholine and sphingomyelin present in the lipid extracts from animal tissues, blood and amniotic fluids were made without interference from other phospholipids or ultraviolet-absorbing material. The method described here is complementary to the high-performance liquid chromatographic method described previously for the analysis of ethanolamine-containing phosphoglycerides and serine-containing phosphoglycerides [Jungalwala, Turel, Evans and McCluer (1975) Biochem. J. 145, 517-526].

Quantitative Analysis of 5-Hydroxymethylfurfural in Linezolid Injection by High Performance Liquid Chromatography

2020 ◽  
Vol 16 (8) ◽  
pp. 1059-1067
Author(s):  
Jéssica Maurício Batista ◽  
Christian Fernandes
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Official Method ◽  
Ph Variation ◽  
Drug Products ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background: Linezolid is a synthetic broad-spectrum antibacterial belonging to the class of oxazolidinones. Linezolid for intravenous infusion is isotonized with dextrose. In acidic environment, the dehydration of dextrose produces furan derivatives, 5-hydroxymethylfurfural (5-HMF) being the main one. The determination of this degradation product is of fundamental importance, since there is evidence it is cytotoxic, genotoxic, mutagenic and carcinogenic. However, there is no official method for the determination of 5-HMF in drug products. Objective: The aim of this study was to develop and validate a high performance liquid chromatographic method to quantify 5-HMF in injection of linezolid. Methods: The chromatographic separation, after optimization, was performed on C18 (150 x 4.6 mm, 5 μm) column. Mobile phase was composed of 14 mM potassium phosphate buffer pH 3.0 ([H+] = 1.0 x 10-3) and methanol in gradient elution at 1.0 mL min-1. The injection volume was 10 μL and detection was performed at 285 nm. Results: The method was optimized and validated, showing selectivity, linearity in the range from 0.075 to 9.0 μg mL-1, precision (RSD ≤ 2.0%), accuracy (mean recovery of 100.07%) and robustness for temperature and pH variation. Conclusion: The method was shown to be adequate to determine 5-HMF in injection containing linezolid in routine analysis.

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