Identification of Human Hemoglobins

1957 ◽  
Vol 3 (1) ◽  
pp. 1-19 ◽  
Author(s):  
C A J Goldberg

Abstract Practical physical and chemical procedures available for the identification of hemoglobins have been discussed. These include electrophoresis, chromatography, alkali denaturation, and ferrohemoglobin solubility. The conditions essential for satisfactory electrophoretic separations have been considered and two procedures for paper electrophoresis at pH 6.5, 7.8, and 8.6 using apparatus for compressed paper strips and for freely suspended horizontal strips are presented. A method for the determination of ferrohemoglobin solubility also has been described.

Author(s):  
O.S. Bezuglova ◽  

Rostov Region belongs to the highly protected natural territories characterized by the continuous plowing. There territories are the only reserves with the soils preserved in their natural state. However, these areas often lack detailed information about the soils quality and composition. Surveying soils on these territories is crucial for determination of their basic physical and chemical properties. The resulted compilation of soil maps could lay a foundation for creating the Red Book of Soils and the formation of a section in the soil-geographical database of the Russian Federation. Subsequently, such information can be used as a background data for the main types of soils in the region. It will be also valuable during monitoring and justification of conservation measures.


2015 ◽  
Vol 63 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Karsten Schacht ◽  
Bernd Marschner

Abstract The use of treated wastewater (TWW) for agricultural irrigation becomes increasingly important in water stressed regions like the Middle East for substituting fresh water (FW) resources. Due to elevated salt concentrations and organic compounds in TWW this practice has potential adverse effects on soil quality, such as the reduction of hydraulic conductivity (HC) and soil aggregate stability (SAS). To assess the impact of TWW irrigation in comparison to FW irrigation on HC, in-situ infiltration measurements using mini disk infiltrometer were deployed in four different long-term experimental orchard test sites in Israel. Topsoil samples (0-10 cm) were collected for analyzing SAS and determination of selected soil chemical and physical characteristics. The mean HC values decreased at all TWW sites by 42.9% up to 50.8% compared to FW sites. The SAS was 11.3% to 32.4% lower at all TWW sites. Soil electrical conductivity (EC) and exchangeable sodium percentage (ESP) were generally higher at TWW sites. These results indicate the use of TWW for irrigation is a viable, but potentially deleterious option, as it influences soil physical and chemical properties.


Еnzymes are able to effectively interact with various organophosphorus compounds (OPC), entering into (bio)chemical reactions with them. Changes in the initial activity of enzymes as a result of their inhibition by OPC, the formation of OPC degradation products under the action of hydrolytic enzymes, etc. can be determined using different physical and chemical methods and used in bioanalytic systems to determine the concentrations of OPC. The purpose of the review is to analyze the main scientific results achieved over the past 10 years in the development of analytical systems based on enzymes intended for the determination of OPC. It is shown in the article, that the requirements for the sensitivity of biosensors are based on the norms of the content of the analyzed substances detected in/at the objects of mandatory control. The cholinesterases compose a basis for the development of the largest number of ultra-sensitive biosensors, although other enzymes can be successfully used as a biosensitive element. The most technologically advanced solution that is close to the practical implementation seems to be bioanalytical systems using immobilized enzymes. Improving the detection limits of the OPC can be achieved by using nanoobjects together with modern methods of signal transducers, for example, with nanomechanical detectors and signal converters. This combination of technical solutions ensures the sensitivity of the OPC analysis up to pg/l. At present, «reagentless» systems have received significant development, which have become the basis for the production of a large number of commercially available strips for the express determination of OPC. Modern demands stimulate the rapid development of portable and, especially, wearable biosensors that can be attached to various surfaces, including a clothing. The progress in the development of affine amino acid sequences, in the future, will allow the creation of enzyme biosensors on any surface.


2009 ◽  
Vol 97 (4-5) ◽  
Author(s):  
K. M. Dziewinska ◽  
A. M. Peters ◽  
J. A. La Verne ◽  
P. Martinez ◽  
J. J Dziewinski ◽  
...  

AbstractDensity measurements of plutonium metal and its alloys are performed at Los Alamos National Laboratory (LANL) using a technique based on the Archimedes principle. The goal is to find and characterize a fluid for density determination of large objects made of plutonium to replace the currently used monobromobenzene. Physical and chemical properties must be considered while selecting the fluid. Chemical properties of the fluid must ensure low corrosion rates and good resistance to radiological decomposition. The study was carried on to evaluate the corrosion rates of Pu metal cause by FC-43 and the FC-43 decomposition products of radiolysis. The results of these studies proved favourable for this application. The evaluation of the surface reactions between FC-43 and Pu metal will be conducted in the near future.


2017 ◽  
Vol 10 (3) ◽  
pp. 1 ◽  
Author(s):  
I. T. S. Viana ◽  
G. V. A. Fonseca ◽  
G. H. Bueno ◽  
J. O. F. Melo

In recent years there has been an important increase in the production of grape derivatives such as integral grape juice. Thus, it was intended with the accomplishment of this study to evaluate the composition of the grape juice of the grape cultivars: Bordô, Concord e Isabel in the composition of integral grape juice from the Bento Gonçalves-RS. The samples were evaluated for classical analysis:°Brix, pH, titratable acidity, ratio Brix / titratable acidity made through methods, physics, physical chemistry and chemistry. The mineral elements sodium and potassium analyzed by flame emission and color intensity by colorimetry. For the statistical analysis of the data, the Tukey test was applied at the 5% level of significance. The contents of integral grape juice evaluated, SS, A Tand minerals presented according to the legislation, the pH was within the comparative average with other works. The determination of the color demonstrated the need to make cuts.


2014 ◽  
Vol 13 (4-8) ◽  
pp. 147-155
Author(s):  
N. Alifar ◽  
A.R. Zaharah ◽  
C.F. Ishak ◽  
Y. Awang ◽  
B. Khayambash

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