Nephelometry of acute-phase glycoproteins by binding to concanavalin A.

1984 ◽  
Vol 30 (11) ◽  
pp. 1834-1835 ◽  
Author(s):  
E M Pitkänen ◽  
T Palosuo ◽  
K Aho ◽  
T Putus-Tikkanen ◽  
R von Essen
Keyword(s):  
Acute Phase ◽  
Concanavalin A ◽  
Binding Assay ◽  
Normal Values ◽  
C Reactive Protein ◽  
Con A ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Inflammatory Joint Diseases

Abstract Nephelometry of serum acute-phase glycoproteins by binding to concanavalin A (con-A) was compared with assays for haptoglobin and alpha 1-acid glycoprotein, and for C-reactive protein. The cutoff points for positive reactions were determined on the basis of results for a random sample (n = 130) from a middle-aged population. The sensitivity of the con-A binding assay compared favorably with that of individual acute-phase glycoproteins in a follow-up cohort of 198 patients with inflammatory joint diseases. Unlike the case in many individual acute-phase glycoprotein assays, the distribution of con-A binding values in healthy subjects is remarkably symmetrical, allowing an easy distinction between abnormal and normal values.

scholarly journals The Acute Phase Proteins Reaction in Children Suffering from Pseudocroup

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Beata Pucher ◽  
Magdalena Sobieska ◽  
Michal Grzegorowski ◽  
Jaroslaw Szydlowski
Keyword(s):  
Acute Phase ◽  
Inflammatory Reaction ◽  
Acute Phase Proteins ◽  
Control Group ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Persistent Inflammation ◽  
Alpha 1 Antitrypsin

The aim of the study was to evaluate the inflammatory reaction in children with pseudocroup and compare it with other laryngological diseases according to the available literature data. The study group included 51 children hospitalized because of pseudocroup. The measurements of the acute phase proteins (APP), such as C-reactive protein (CRP), alpha-1-antitrypsin (AT), alpha-1-antichymotrypsin (ACT), alpha-1-acid glycoprotein (AGP), ceruloplasmin (Cp), transferrin (Tf), alpha-2-macroglobulin (A2M), and haptoglobin (Hp) were obtained at 3 time points. The glycosylation profiles of AGP, ACT, and Tf were completed. An increased AGP level was observed in girls. The AGP glycosylation revealed the advantage of the W0 variant over the W1 variant. W1 and W2 were decreased in boys. W3 emerged in boys. The Tf concentration and T4 variant were lower compared to the control group. The A2M level was lower after treatment. The Hp and AT levels were decreased a few weeks later. The ACT glycosylation revealed a decrease of the A4 variant in boys. In conclusion, the inflammatory reaction during pseudocroup was of low intensity. The APP glycosylation suggested a chronic process. In a follow-up investigation, no normalization of the parameters was noted, but signs of persistent inflammation were observed.

scholarly journals Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

1986 ◽  
Vol 32 (5) ◽  
pp. 743-747 ◽  
Author(s):  
J Braun ◽  
T Schultek ◽  
K F Tegtmeier ◽  
A Florenz ◽  
C Rohde ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Immunoglobulin A ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Coefficients Of Variation ◽  
Alpha 1 Antitrypsin ◽  
Heel Prick ◽  
Secretory Immunoglobulin

Abstract We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

Elevation of FVIII: C in Venous Thromboembolism Is Persistent and Independent of the Acute Phase Response

2000 ◽  
Vol 83 (01) ◽  
pp. 10-13 ◽  
Author(s):  
Andrew Mumford ◽  
Richard Manning ◽  
Michael Laffan ◽  
James O’Donnell
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Acute Phase Reaction ◽  
Phase Reaction ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Significant Difference ◽  
Venous Thromboembolic

SummaryRecent literature has suggested a role for elevated FVIII:C in venous thromboembolic disease (VTED). However since FVIII:C is known to rise in response to an acute phase reaction, it is difficult to determine whether the increased FVIII:C precedes the thrombosis or represents a secondary reactive phenomenon. In an attempt to address this question, we followed 35 patients with confirmed VTED, raised FVIII:C level (>1.5 iu/ml) and no other thrombotic tendency. Serial measurements of FVIII:C, vWF:Ag, C-reactive protein and fibrinogen were performed. We hypothesized that a persistent increase in FVIII:C in the absence of any other measures of ongoing acute phase response, would support the idea that elevation of FVIII:C is a constitutional phenomenon.Of this initial group, 94% continued to have an elevated FVIII:C level throughout the period of follow up (median 8 months; range 3 to 39 months), with no significant difference between the FVIII:C levels determined at first estimation and those obtained during follow up (p = 0.58). Conversely, only 18% had evidence of an acute phase reaction when first assessed, and nonparametric ranking analysis demonstrated no correlation between FVIII:C and either C-reactive protein or fibrinogen (p = 0.315 and 0.425 respectively).We conclude that increased FVIII:C levels following VTED are persistent, independent of the acute phase reaction, and thus may represent a constitutional risk factor for VTED.

Changes in Serum Inflammatory Proteins during Peritonitis in CAPD Patients

1983 ◽  
Vol 3 (4) ◽  
pp. 201-204 ◽  
Author(s):  
Janet Gilmour ◽  
Rosemary Tymiansky ◽  
Andreas Pierratos ◽  
Stephen Vas ◽  
Michael Kline ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Reactant ◽  
Serum Concentrations ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Phase Reactant ◽  
Inflammatory Proteins ◽  
Baseline Values ◽  
Normal Controls

The serum concentrations of IgG, IgA, IgM, C3, C4, haptoglobin, tranferrin, αl-antitrypsin, αl-acid glycoprotein, and C-reactive protein were measured in 14 CAPD patients during the course of peritonitis and at one, two and three months thereafter. Of these parameters only the CRP showed consistent changes paralleling the course of the peritonitis. The CRP baseline values in CAPD patients (second and third month after peritonitis) were similar to those found in non-dialysed normal controls, suggesting that the CRP changes, which were observed, can be attributed to the peritonitis; CRP may be a valid acute phase reactant during peritonitis in PD patients.

scholarly journals Impact of Roux-en-Y gastric bypass on lipid and inflammatory profiles

2015 ◽  
Vol 42 (5) ◽  
pp. 305-310 ◽  
Author(s):  
Cleiton da Silva Oliveira ◽  
Bruna Teles Soares Beserra ◽  
Raphael Salles Granato Cunha ◽  
Ana Gabriela Estevam Brito ◽  
Rafaella Cristina Dimbarre de Miranda ◽  
...  
Keyword(s):  
Gastric Bypass ◽  
Total Cholesterol ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Plasma Concentrations ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Significant Difference ◽  
A Prospective Study

Objective: To evaluate the behavior of acute phase proteins and lipid profile in patients undergoing Roux-en-Y gastric bypass. Methods : We conducted a prospective study, consisting of three moments: M1 - preoperative (24 hours before surgery); M2 - 30 days after surgery; and M3 - 180 days after surgery. We carried measured height and BMI, as well as determined the concentrations of acute phase proteins (C-reactive protein (CRP), albumin and Alpha-1-acid glycoprotein) and total cholesterol, LDL-c, HDL-c and triacylglycerol. Results : participants comprised 25 individuals, with a mean age of 39.28 ± 8.07, 72% female. At all times of the study there was statistically significant difference as for weight loss and BMI. We found a significant decrease in CRP concentrations between the moments M1 and M3 (p = 0.041) and between M2 and M3 (p = 0.018). There was decrease in Alpha-1-GA concentrations between M1 and M2 (p = 0.023) and between M1 and M3 (p = 0.028). The albumin values increased, but did not differ between times. Total cholesterol and triacylglycerol decreased significantly ay all times. LDL-c concentrations decreased and differed between M1 and M2 (p = 0.001) and between M1 and M3 (p = 0.001). HDL-c values increased, however only differing between M1 and M2 (p = 0.050). Conclusion : Roux-en-Y gastric bypass promoted a decrease in plasma concentrations of CRP and Alpha-1-acid glycoprotein, improving lipid and inflammatory profiles.

scholarly journals Serum Total Sialic Acid and Lipid-Associated Sialic Acid in Normal Individuals and Patients with Myocardial Infarction, and Their Relationship to Acute Phase Proteins

1993 ◽  
Vol 30 (4) ◽  
pp. 383-386 ◽  
Author(s):  
M Haq ◽  
S Haq ◽  
P Tutt ◽  
M Crook
Keyword(s):  
Myocardial Infarction ◽  
Sialic Acid ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Total Sialic Acid ◽  
Normal Individuals ◽  
The Mean

Serum total sialic acid (TSA) has gained medical interest, particularly as a cardiovascular risk factor and it has been hypothesized that serum levels relate to serum acute phase proteins, some of which are sialylated. We assayed serum TSA and also lipid associated sialic acid (LASA) in SO normal individuals (24 male) and IS subjects (12 male) who had experienced a myocardial infarct. The mean serum TSA in the normal individuals was 2·05 SD 0·38 mmol/L (range 1·16–2·74) and the mean serum LASA was 0.70 SD 0·19 mmol/L (range 0·23–1·03). We also measured five serum acute phase proteins and found a good correlation between these and serum TSA: C-reactive protein, r = 0.52, P<0.001, α-1-antichymotrypsin, r=0·79, P<0·0001, α-2-macroglobulin, r=0·38, P<0·01 and α-1-acid glycoprotein, r=0·32, P<0·05. A significant correlation between plasma TSA and plasma C-reactive protein ( r = 0·47, P<0·04) and also Fibrinogen ( r=0·53, P<0·04) was noted on day one following the myocardial infarction, whereas a significant correlation between plasma TSA and plasma α-1-antichymotrypsin ( r=0·51, P<0·03) and also plasma α-1-acid glycoprotein ( r=0·64, P<0·05) was found on day two following the infarction. Thus it would seem that serum TSA is at least in part related to some of the acute phase proteins in both healthy individuals and those having had a myocardial infarction.

Acute phase proteins in the healing of bone fractures and skin burn wounds

1986 ◽  
Vol 67 (3) ◽  
pp. 208-209
Author(s):  
V. M. Miroshnikov ◽  
L. V. Kugler ◽  
E. I. Doronin ◽  
A. A. Nyrkin
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Bone Fractures ◽  
Trauma Patients ◽  
C Reactive Protein ◽  
Skin Burn ◽  
Burn Wounds ◽  
Reactive Protein ◽  
Selective Analysis

We determined the content of C-reactive protein (CRP) and a2-glycoprotein in the serum of patients with bone fractures (78 patients) and skin burns (26 patients). In fractures irrespective of follow-up period and extent of injury, C-RB was detected in 79.5% of patients, in burns - in 92.3%; a2-glycoprotein - respectively, in 51.3% and 50.0% (without taking into account gender of patients). Selective analysis of cases of a2-glycoprotein detection depending on sex showed that it was detected much more frequently in women: in 77.0% of fractures and 60% of burns. A similar gender demorphism in trauma patients was also observed in the frequency of a2-glycolprotein detection.

scholarly journals Associations between C-reactive protein levels and cognition during the first 6 months after acute psychosis

2018 ◽  
Vol 31 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Farivar Fathian ◽  
Else-Marie Løberg ◽  
Rolf Gjestad ◽  
Vidar M. Steen ◽  
Rune A. Kroken ◽  
...  
Keyword(s):  
Serum Level ◽  
Cognitive Performance ◽  
Acute Phase ◽  
Randomised Trial ◽  
Inverse Association ◽  
Acute Psychosis ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein

AbstractObjectiveInverse relationships between the C-reactive protein (CRP) levels and cognitive performance in acute psychosis have been demonstrated. We aimed to investigate how the serum level and initial change of CRP in acutely admitted patients with psychosis was correlated with cognitive performance during a 6-months follow-up period.MethodsThe study is part of a pragmatic, randomised trial comparing four different second-generation antipsychotic drugs, and consists of 208 acute phase patients recruited at admittance for psychosis. This study reports data for all groups collectively, and does not compare treatment groups. Measurements of CRP and cognitive performance were conducted at baseline (T1) and after 4 weeks on average after inclusion (T2). Cognition was also assessed after 3 months (T3) and 6 months (T4) of follow-up.ResultsGlobal cognition improved during the follow-up period of 6 months, especially in the T1–T2 interval. The different cognitive subdomains showed different time-dependent profiles of improvement, with memory and attention improving significantly also in the later phases. Reduction of the CRP level during the initial follow-up interval (T1–T2) was associated with increased overall cognitive performance in the T2–T4 interval, but not in the T1–T2 interval. For the cognitive subdomains, we found an inverse association between change in CRP level and verbal abilities (T2–T4 interval), and attention (T2–T3 interval).ConclusionThese findings indicate that initial changes in the serum level of CRP in the acute phase of psychosis may predict cognitive function in later phases of the disease.

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