Protective Effect of Caffeic Acid Phenethyl Ester on Cadmium-Induced Liver Toxicity: A Histopathological and Biochemistry Study

In this study, the changes caused by caffeic acid phenethyl ester (CAPE) in histopathological and biochemical parameters in oxidant / antioxidant balance in mice with experimental cadmium toxicity were investigated. In blood samples from mice, plasma paraoxonase (PON) activity, high density lipoprotein (HDL), low density lipoprotein (LDL), total sialic acid (TSA) total antioxidant capacity (TAC), total oxidant capacity (TOC) and oxidative stress index (OSI) ) values ​​were analyzed. In this study, it was found that cadmium intoxication triggered oxidative stress in mice, lowering PON activity, TAC, HDL levels and increasing TSA, LDL, TOC, OSI levels. In the histopathological examination of the liver tissues, focal apoptotic regions, sinusoidal occlusion and irregularity were found in the cadmium group, while no significant change was found in the other groups. According to these results, it has been shown that CAPE can significantly prevent biochemical and histopathological changes due to cadmium damage.

Sialic acid as a potential biomarker for cardiovascular disease, diabetes and cancer

Cardiovascular disease (CVD), diabetes and cancer pose increasing global healthcare burdens. New biomarkers could enable earlier diagnosis of these diseases, leading to more effective treatment and lower associated healthcare burden. Elevated sialic acid concentration in plasma and serum has been positively correlated with the presence of CVDs, diabetes and the development of malignant tumors. This article reviews the use of total sialic acid (TSA), bound sialic acid (BSA) and free sialic acid (FSA) as potential biomarkers for these diseases and makes a comparison with existing markers. Elevated sialic acid has been shown to be indicative of the pathogenesis of CVD, diabetes and malignant tumors. While not a specific marker for one disease there is promise in utilizing sialic acid as a marker for monitoring disease progression and effectiveness of treatment programs.

Sialoglyco-Conjugate Abnormalities, IL-6 Trans-Signaling and Anti-Ganglioside Immune Response—Potential Interferences in Lupus Nephritis Pathogenesis

We have investigated glycoconjugates sialization profile, endogen synthesis rate of antiganglioside antibodies (AGA), IL-6 signaling pathways correlated with activity disease in systemic lupus erythematous (SLE) and lupus nephritis (LN). Material and methods. A case-control study was developed and included 109 patients with SLE with or without renal impairment, 32 patients with IgA nephropathy and 60 healthy volunteers, clinically and paraclinically monitored. The following parameters were evaluated in volunteers serum: total sialic acid (TSA), orosomucoids, lipid bound sialic acid (LSA), interleukin-6 (IL-6), soluble factors IL-6R, gp130, anti –GM1, -GM2, -GM3, -GD1a, -GD1b, -GT1b, -GQ1b antigangliosides antibodies of IgG and IgM type. Results. Experimental data analysis showed: increase in synthesis rhythm of sialoglyco-conjugated in SLE (TSA increased in SLE and LN compared to control), accelerated catabolism of LSA in LN (LSA/TSA ratio was higher in SLE and LN than in control group), overexpression of IL-6 mediated trans-signaling (sIL-6R/sgp 130 ratio was subunit in SLE and IgA nephropathy and superunit in LN), large AGA profile synthesis of IgM isotype (over 45.1% in SLE and over 20.7% in LN). Conclusions. Hypersialization, accelerated glycosphingolipids degradation, IL-6 trans-signaling amplify and AGA pattern could represent essential mechanisms in LN pathogenesis.

Low vitamin B12 level in relation to trace element, total sialic acid and antioxidant enzymes in children with vitamin B12 deficiency anemia

In this study, children with vitamin B12 deficiency anemia (V-B12DA) and control subjects were evaluated for erythrocyte glutathione peroxidase, catalase and superoxide dismutase enzyme activities, glutathione, malondialdehyde, serum total sialic acid, total antioxidant status, cobalt, chromium, copper, selenium, vanadium, zinc, iron, lead, magnesium, calcium, sodium, potassium, chloride, phosphorus levels, and the associations of these variables were assessed. The study included 50 children with V-B12DA and 50 control subjects. It was found that the V-B12DA group was significantly lower than the control group, with regard to the mean±the standard error of the mean levels of cobalt (0.089±0.009; 0.058±0.0063 μmol/L, p<0.01), selenium (2.19±0.087; 1.88±0.057 μmol/L, p<0.01), vanadium (1.31±0.053; 1.18±0.035 μmol/L, p<0.05), magnesium (3.02±0.15; 2.73±0.068 μmol/L, p<0.05), zinc (50.76±1.96; 42.23± 1.53 μmol/L, p<0.001), and vitamin B12 (427.20±21.45; 157.08±3.96 pg/mL, p<0.001). Moreover, a significant elevation in total sialic acid (1.44±0.050; 1.61±0.043 mmol/L, p<0.01), and mean corpuscular volume (MCV) (75.37±0.95; 79.91±1.14 fL, p<0.01). It was observed that in the V-B12DA, significantly linear correlations were observed between cobalt – vitamin B12 (r=0.334; p=0.025), vanadium – MCV (r=0.315; p=0.017), vitamin B12 – MCV (r=−0.297; p=0.026). The findings of the study indicated that the levels of cobalt, vanadium significantly associated with traditional vitamin B12-deficiency parameters. Vitamin B12 and MCV should be measured together with cobalt, vanadium for monitoring the vitamin B12 deficiency anemia.

Serum levels of total sialic acid and lipid profile with the severity of the vessel in patients with non-smoker and diabetic coronary artery disease

Background: Coronary Artery Disease (CAD) is a major cause of mortality in most countries. Many risk factors such as high blood pressure, hyperlipidemia, diabetes, age, sex, obesity, smoking, and family history play a role in CAD. The aim of this study was to evaluate the concentration of Total Sialic Acid (TSA) and Lipid Profiles (LP) with the Severity of the Vessel in patients with non-smoker and diabetic CAD, so that by measuring these parameters, effective help for diagnosis and prevention for healthy people Prone to CAD, and also control the treatment of patients. Methods: In this study, 200 individual including 160 patient and 40 control group were considered. All patient groups were non-smokers and diabetic. Patients were divided into 4 groups according to the results of angiography: Patients with Normal angiography (n = 40) with one eclipse (n = 40), patients with double stenosis (n = 40) and patients with eclipse Three vessels (n = 40). The control group was chosen from people who had no history of CAD and other diseases. The lipid profile was measured by standard methods and serum total sialic acid was measured by ELISA method. Results: There was no significant difference between the two groups in age and sex, but there was a significant difference in family history (p<0.05). There was a significant difference in serum glucose level between the patient and the control group (p<0.05), Also hs-CRP serum levels were normal in two patient and control groups. Serum levels of cholesterol, triglyceride and LDL in patient group were significantly higher than that of the control group but HDL serum level was adverse (p <0.05), Also TSA serum level in the patient group was significantly higher than the control group (p<0.05). Conclusion: Serum Total Sialic Acid level in non-smoker and diabetic CAD patients has a significant increase compared to the control group. It seems that the above biochemical parameters contribute significantly to the development and progression of atherosclerosis and CAD, by which timely measurements of these parameters in healthy individual probably can be helpful in preventing and improving CAD and controlling the treatment of patients.

Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma

Posttranslational modifications are dynamic enzymatic-mediated processes, regulated in time and space, associated with cancer development. We aimed to evaluate the significance of posttranslational modifications in the pathogenesis of clear cell renal cell carcinoma. The authors developed a prospective, observational study during a period of three years and included 55 patients with localized renal cell carcinoma and 30 heathy subjects. Glycosylation, nitration and carbonylation, thiol-disulfide homeostasis, methylation, phosphorylation and proteolytic cleavage were evaluated in the serum of the evaluated subjects in the present study. Our results showed some characteristics for early ccRCC: high production of cytokines, substrate hypersialylation, induced nitrosative and carbonylic stress, arginine hypermethylation, thiol/disulfide homeostasis (TDH) alteration, the regulatory role of soluble receptors (sRAGE, sIL-6R) in RAGE and IL-6 signaling, the modulatory effect of TK-1and TuM2-PK in controlling the level of phosphometabolites in neoplastic cells. These data could be the initial point for development of a panel of biomarkers such as total sialic acid, orosomucoids, nitrotyrosine, carbonylic metabolites, ADMA, SDMA, and thiol-disulfide equilibrium for early diagnosis of ccRCC. Moreover, they could be considered a specific disease PTM signature which underlines the transition from early to advanced stages in this neoplasia, and of a therapeutic target in kidney oncogenesis.

Estimation of Total and Lipid Bound Sialic acid in oral leukoplakia and oral squamous cell carcinoma patients

Tumour markers are biochemical substances released through tumour cells. They are considered as the rationale or consequence of the carcinogenesis process. Neoplasms often have an increased concentration of sialic acid on the tumour cell surface and are shed or secreted by some of these cells, which increase the concentration in blood. To determine serum levels of total sialic acid (TSA), lipid-bound sialic acid (LBSA), in patients of oral Leukoplakia (LP) and oral squamous cell carcinoma (OSCC). The study comprises 75 subjects which include 25 cases of LP, 25 cases of OSCC and 25 cases of healthy individuals as control. 10 ml intravenous blood was collected under aseptic condition, and biochemical analysis of total sialic acid and lipid-bound sialic acid was carried out by spectrophotometer. We observed levels of TSA and LBSA significantly increased in LP and OSCC as compared to a healthy control group. The increased level of TSA and LBSA in LP helps to determine the early stage of the disease. Further differentiation in grades of OSCC is also possible by these biochemical markers. Thus serum levels of TAS, LBSA can be used as diagnostic and prognostic markers.