Immunoreactive phospholipase A2 in serum in acute pancreatitis and pancreatic cancer.

1985 ◽  
Vol 31 (7) ◽  
pp. 1116-1120 ◽  
Author(s):  
T J Nevalainen ◽  
J U Eskola ◽  
A J Aho ◽  
V T Havia ◽  
T N Lövgren ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Acute Pancreatitis ◽  
Phospholipase A2 ◽  
Clinical Severity ◽  
Pancreatic Acinar Cells ◽  
Alcoholic Pancreatitis ◽  
Time Resolved ◽  
The Mean ◽  
Immunoreactive Phospholipase A2 ◽  
Severity Of The Disease

Abstract Immunoreactive phospholipase A2 (EC 3.1.1.4) was measured by a new sensitive time-resolved fluoroimmunoassay in the serum of 58 healthy subjects and 103 patients with acute pancreatitis. Patients with acute pancreatitis were grouped according to the etiology and clinical severity of the disease. The mean phospholipase A2 concentration in the reference (healthy) group was 5.5 (SD 1.9) micrograms/L. In acute pancreatitis the mean phospholipase A2 concentration was increased on the first day after hospital admission in all groups, and returned to normal somewhat more slowly than did serum amylase, especially in the patients with severe alcoholic pancreatitis. In this latter group the mean concentration of serum phospholipase A2 on the first day was 42.6 (SD 29.5) micrograms/L. In patients with pancreatic cancer, serum phospholipase A2 was 29.2 (SD 21.3) micrograms/L. The phospholipase A2 and amylase values were closely associated in all groups. The clinical sensitivities were 90.9% for severe alcoholic pancreatitis and 87.5% for pancreatic cancer. Immunochemical determination of phospholipase A2 in serum provides fast and specific detection of injury to pancreatic acinar cells. In addition to the early diagnosis of acute pancreatitis, follow-up determinations of phospholipase A2 seem to be useful in differentiating between mild and severe forms of pancreatitis.

Diagnostic value of immunoreactive phospholipase A2 in acute pancreatitis

1989 ◽  
Vol 67 (3) ◽  
pp. 183-185 ◽  
Author(s):  
P. Malfertheiner ◽  
T. Nevalainen ◽  
W. Uhl ◽  
H. Schädlich ◽  
M. Büchler
Keyword(s):  
Acute Pancreatitis ◽  
Phospholipase A2 ◽  
Diagnostic Value ◽  
Immunoreactive Phospholipase A2

Time-resolved fluoroimmunoassay of human pancreatic phospholipase A2.

1983 ◽  
Vol 29 (10) ◽  
pp. 1777-1780 ◽  
Author(s):  
J U Eskola ◽  
T J Nevalainen ◽  
T N Lövgren
Keyword(s):  
Phospholipase A2 ◽  
Solid Phase ◽  
High Sensitivity ◽  
Fluorometric Detection ◽  
Time Resolved ◽  
Antibody Technique ◽  
Pancreatic Phospholipase ◽  
Pancreatic Phospholipase A2 ◽  
Immunoreactive Phospholipase A2 ◽  
Solid Phase Assay

Abstract We describe an immunofluorometric assay for human pancreatic phospholipase A2 based on time-resolved fluorescence. The labeled antibody technique in combination with the time-resolved 1-s fluorometric detection of the europium label, which essentially eliminates all background fluorescence, resulted in a high sensitivity (20 ng/L) and a wide (5000-fold) linear range. Nonspecific binding was minimized by treating the solid-phase antibody with NaSCN before coating, to remove endogenous antigen, and by immunosorbent purification of the antibody before labeling with europium. This is a one-incubation, multi-site, solid-phase assay on polystyrene microtiter strips, even though a polyclonal antibody was used. As measured by this assay, activity of immunoreactive phospholipase A2 was found to be above normal in sera of patients suffering from acute pancreatitis.

scholarly journals Serum TLR9 and NF-κB Biochemical Markers in Patients with Acute Pancreatitis on Admission

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Erdal Demirtas ◽  
İlhan Korkmaz ◽  
Kıvanç Cebecioğlu ◽  
Mustafa Ayan ◽  
Esin Demirtaş ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Biochemical Markers ◽  
Inflammatory Process ◽  
Clinical Severity ◽  
Control Group ◽  
Discriminative Ability ◽  
Cutoff Value ◽  
B Values ◽  
Patient Groups ◽  
The Mean

Aim. The aim of this study was to investigate the serum TLR9 and NF-κB levels in patients for the diagnosis and prognostication of AP in the emergency department. Methods. In the current study, we looked at the TLR9 and NF-κB pathways in a cohort of 45 acute pancreatitis patients and compared them with the control group. We also divided the patient groups as mild-moderate or severe and compared the biomarker levels between the groups. Results. Of the patients with acute pancreatitis, 22 (49%) were male and 23 (51%) were female. The mean age of the patient group was 62 years, with a range of 25–95 years. The control group consisted of 19 (43.1%) male and 25 (56.9%) female patients. The serum TLR9 and NF-κB values were significantly higher than those of the control group [1104.44 ± 339.20 vs. 702.08 ± 203.94; p<0.001 and 8.04 ± 1.76 vs. 4.76 ± 1.13; p<0.001, respectively]. We found that TLR9 and NF-κB had a significant discriminative ability, while the cutoff value for TLR9 was 950.4, with a sensitivity of 73% and specificity of 93% (p<0.001), and the cutoff value for NF-κB was 6.32, with a sensitivity of 89% and specificity of 100% (p<0.001). Conclusion. We demonstrated that the TLR9 and NF-κB pathway is activated in acute pancreatitis and increases the inflammatory process. This may help to further understand the pathogenesis of disorder, diagnosis, and clinical severity. We proposed that blockage of these inflammatory pathways may play a role in the prevention of the disease progression and development of inflammatory complications.

scholarly journals Clinical significance of serum amylase and glucose level in organophosphorus poisoning

2019 ◽  
Vol 2 (3) ◽  
pp. 121-125
Author(s):  
Manoj Koirala ◽  
Bishow Raj Baral ◽  
Buddhisagar Lamichanne
Keyword(s):  
Acute Pancreatitis ◽  
Glucose Level ◽  
Serum Amylase ◽  
Serum Glucose ◽  
Clinical Severity ◽  
Serum Glucose Level ◽  
Amylase Level ◽  
Serum Amylase Level ◽  
Organophosphorus Poisoning ◽  
The Mean

Background: Organophosphorous (OP) poisoning is a common problem in country like Nepal where agriculture is the backbone of the economy. The primary mechanism of action is inhibition of acetylcholinesterase (ACHE). Organophosphorus poisoning is a possible cause of acute pancreatitis along with alternation of glucose metabolism. Materials and Method: This was a hospital based cross-sectional comparative study. The level of serum amylase and glucose were measured in thirty-eight OP poisoned patients at the time of admission, before discharge and were compared. Results: Amongst 38 patients, Majority had mild OP poisoning 27(71.1%) as per Peradeniya Organophosphorus Poisoning (POP) score whereas 9(23.7%) had moderate and 2(5.2%) had severe poisoning. The mean initial serum amylase level in patients with mild poisoning was 152±73.92 (IU/L)(p=0.000), in moderate poisoning was 213.38±69.39 (IU/L) (p=0.223) and in severe poisoning was 171.33±107.22(IU/L) (p=0.259). There was increase in serum amylase level in all patients with poisoning but level did not increase in proportion to increase with severity of POP score. The mean initial serum glucose level in mild poisoning was 132.48±37.73 (Mg/dl) (p=0.024), in moderate poisoning was 139±44.59 (Mg/dl) (p=0.033) and in severe poisoning was 174±23.38 (Mg/dl) (p=0.22). The serum glucose level increased as the severity of POP score increased. The serum amylase and glucose levels in recovering patients showed a tendency to decrease to their normal values. No patient had developed acute symptomatic pancreatitis. Conclusion: Serum amylase and glucose level were increased in all patients with OP poisoning but didn’t correlate with the clinical severity. Routine measurement of serum amylase in patients of OP poisoning has little value in the absence of clinical acute pancreatitis.

scholarly journals Comprehensive analysis of microRNA signature of mouse pancreatic acini: overexpression of miR-21-3p in acute pancreatitis

2016 ◽  
Vol 311 (5) ◽  
pp. G974-G980 ◽  
Author(s):  
Ajay Kumar Dixit ◽  
Anne E. Sarver ◽  
Zuobiao Yuan ◽  
John George ◽  
Usman Barlass ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Mouse Models ◽  
Expression Profile ◽  
Mirna Expression ◽  
Acinar Cells ◽  
Mirna Expression Profile ◽  
Comprehensive Analysis ◽  
Pancreatic Acinar Cells ◽  
Pancreatic Acini ◽  
Severity Of The Disease

In the current study, we have characterized the global miRNA expression profile in mouse pancreatic acinar cells and during acute pancreatitis using next-generation RNA sequencing. We identified 324 known and six novel miRNAs that are expressed in mouse pancreatic acinar cells. In the basal state, miR-148a-3p, miR-375-3p, miR-217-5p, and miR-200a-3p were among the most abundantly expressed, whereas miR-24-5p and miR-421-3p were the least abundant. Treatment of acinar cells with caerulein (100 nM) and taurolithocholic acid 3-sulfate [TLC-S (250 μM)] induced numerous changes in miRNA expression profile. In particular, we found significant overexpression of miR-21-3p in acini treated with caerulein and TLC-S. We further looked at the expression of miR-21-3p in caerulein, l-arginine, and caerulein + LPS-induced acute pancreatitis mouse models and found 12-, 21-, and 50-fold increased expression in the pancreas, respectively. In summary, this is the first comprehensive analysis of global miRNA expression profile of mouse pancreatic acinar cells in normal and disease conditions. Our analysis shows that miR-21-3p expression level correlates with the severity of the disease.

scholarly journals Clinical profile, degree of severity and underlying factors of acute pancreatitis among a group of Bangladeshi patients

2018 ◽  
Vol 12 (1) ◽  
pp. 6-10
Author(s):  
Indrajit Kumar Datta ◽  
Md Nazmul Haque ◽  
Tareq M Bhuiyan
Keyword(s):  
Acute Pancreatitis ◽  
Gall Stone ◽  
Clinical Findings ◽  
Clinical Profile ◽  
Clinical Feature ◽  
Specific Clinical Feature ◽  
The Mean ◽  
Severity Of The Disease ◽  
Degree Of Severity ◽  
Recorded Diagnosis

Background and objectives: Acute pancreatitis is a common condition for hospital admission. In Bangladesh, no study has yet investigated the clinical profile, degree of severity and underlying factors of acute pancreatitis. The aim of the present study was to determine the clinical profile, degree of severity and underlying factors of acute pancreatitis in a cohort of Bangladeshi patients.Methods: This prospective study was conducted from April 2016 to March 2017 on patients admitted with acute pancreatitis at Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM) General Hospital, Dhaka, Bangladesh. History and clinical features of each patient was systematically recorded. Diagnosis of acute pancreatitis was made by clinical findings, serum amylase and lipase levels (> 3 times the upper limit of normal values), evidences of acute pancreatitis by ultrasonography and computed tomography (CT). Severity of acute pancreatitis was classified according to the revised version of Atlanta classification.Results: A total of 40 patients with acute pancreatitis were enrolled in the study. Male and female were equally distributed. The mean age was 44.3±2.7 years. Among 40 cases, 26 (65.0%) and 14 (35%) had moderate and severe acute pancreatitis respectively. No specific clinical feature including ascites or pleural effusion was found significantly related to severity of the disease. Gall stone and metabolic (hypertriglyceridaemia/hypercalcemia) causes were present in 62.5% cases, but none had significant association with the severity of the disease.Conclusion: The present study has demonstrated that no specific observed clinical feature or underlying factor was related to the degree of severity of acute pancreatitis in a cohort of Bangladeshi patients.IMC J Med Sci 2018; 12(1): 6-10

Pancreatic gene expression during the initiation of acute pancreatitis: identification of EGR-1 as a key regulator

2003 ◽  
Vol 14 (1) ◽  
pp. 59-72 ◽  
Author(s):  
Baoan Ji ◽  
Xue-qing Chen ◽  
David E. Misek ◽  
Rork Kuick ◽  
Samir Hanash ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Rt Pcr ◽  
Novel Genes ◽  
In Vivo Models ◽  
Severity Of The Disease ◽  
Deficient Mice

We hypothesized that genes expressed in pancreatic acinar cells during the initiation of acute pancreatitis determine the severity of the disease. Therefore, we utilized microarrays to identify those genes commonly induced in rat pancreatic acinar cells within 1–4 h in two in vivo models, caerulein and taurocholate administration. This strategy yielded 51 known genes representing a complex array of molecules, including those that are likely to either reduce or increase the severity of the disease. Novel genes identified in the current study included ATF3, BRF1, C/EBPβ, CGRP, EGR-1, ephrinA1, villin2, ferredoxin, latexin, lipocalin, MKP-1, NGFI-B, RhoA, tissue factor (TF), and syndecan. To validate these microarray results, the role of EGR-1 was further investigated using quantitative RT-PCR, Western blotting, and immunocytochemistry. EGR-1 expression occurred within acinar cells and correlated with the development of caerulein-induced acute pancreatitis in rats. Furthermore, the levels of the inflammation-related genes MCP-1, PAI, TF, IL-6, and ICAM-1 and the extent of lung inflammation were reduced during the initiation of caerulein-induced acute pancreatitis in EGR-1-deficient mice. Thus this study identified EGR-1 and several other novel genes likely to be important in the development and severity of acute pancreatitis.

scholarly journals Epidemiology and Etiology of Acute Pancreatitis in Urban and Suburban Areas in Shanghai: A Retrospective Study

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Junjie Fan ◽  
Ling Ding ◽  
Yingying Lu ◽  
Junyuan Zheng ◽  
Yue Zeng ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Retrospective Study ◽  
Urban Area ◽  
Suburban Area ◽  
Alcoholic Pancreatitis ◽  
Onset Age ◽  
Old Patients ◽  
Suburban Areas ◽  
Male Patients ◽  
The Mean

Aim. To investigate the epidemiology, etiology, and severity of acute pancreatitis (AP) in urban and suburban areas of Shanghai in 2011 and 2016. Methods. A retrospective study of patients admitted to Shanghai General Hospital (urban and suburban campuses) with AP in 2011 and 2016 was undertaken. Patients were divided into acute biliary pancreatitis (ABP), hypertriglyceridemic pancreatitis (HTGP), alcoholic pancreatitis, and pancreatitis of other causes according to etiology. Severity of AP was divided into mild AP (MAP), moderately severe AP (MSAP), and severe AP (SAP). Results. AP patients in the suburban area increased more rapidly than those in the urban area. The mean onset age of AP in the urban area in 2016 was older than that in the suburban area (p<0.05). The suburban patients in 2016 have significantly younger mean onset age than those in 2011 (p<0.05). HTGP incidence in suburban patients increased from 2011 to 2016, which changed little in the urban area. Urban females were more likely to develop HTGP than suburban ones in 2011, which reversed in 2016. As to the male patients, the incidence of HTGP increased in both urban and suburban areas. Nonelderly (<60 years old) patients had higher HTGP incidence than elderly ones in both 2011 and 2016. The descending trend of SAP in the suburban area was more obvious than that in the urban area. The length of hospitalization decreased from 2011 to 2016, especially in SAP patients. Conclusions. AP patients increased more rapidly in the suburban area of Shanghai with younger onset age. The incidence of HTGP increased significantly in the suburban area, reminding of the prevention and screening of HTG.

Clinico-Haematological Alterations in Goats Affected with Ruminal Acidosis

2017 ◽  
Vol 13 (02) ◽  
Author(s):  
P. R. Chavelikar ◽  
G. C. Mandali ◽  
D. M. Patel
Keyword(s):  
Clinical Signs ◽  
Clinical Manifestations ◽  
Nasal Discharge ◽  
Mean Values ◽  
Ruminal Acidosis ◽  
Reduced Body ◽  
Muscle Twitching ◽  
Haematological Profile ◽  
The Mean ◽  
Severity Of The Disease

Ruminal acidosis is one of the most important clinical emergencies in sheep and goats resulting into high mortality rate. In the present study, eight healthy farm goats and 24 goats presented to the TVCC of the college with clinical signs of ruminal acidosis like anorexia, tympany, increased pulse and respiratory rate, reduced body temperature, doughy rumen, enteritis, oliguria, grinding of teeth, purulent nasal discharge, muscle twitching, arched back, dehydration and recumbency with rumen liquor pH below 6 were examined for haematological alterations using autohaematoanalyzer. Among various haematological parameters evaluated from acidotic goats, the mean values of Hb (12.21±0.17 vs. 10.86±0.15 g/dl), TEC (14.28±0.16 vs. 12.04±0.36 ×106/ μl), TLC (13.43±0.11 vs. 11.11±0.27 ×103/μl), PCV (36.91±0.53 vs. 29.88±0.55%), neutrophils (64.54±0.93 vs. 28.13±0.92%), MCV (23.38±0.37 vs. 19.38±1.34 fl) and MCH (7.03±0.08 vs. 6.31±0.25 pg) were found significantly increased, while the mean values of lymphocytes (28.00±0.82 vs. 65.38±0.80%) and MCHC (24.55 ±0.26 vs. 34.88±0.97 g/dl) were decreased significantly from the base values of healthy goats. It was concluded that ruminal acidosis induced due to accidental heavy ingestion of readily fermentable carbohydrate rich grains and food waste significantly altered the haematological profile concurrent with clinical manifestations in goats, and hence can be used to assess the severity of the disease.

scholarly journals Serum Phospholipase A2 in Canine Acute Pancreatitis

1983 ◽  
Vol 24 (4) ◽  
pp. 477-487
Author(s):  
E. Westermarck ◽  
E. Rimaila-Pärnänen
Keyword(s):  
Acute Pancreatitis ◽  
Phospholipase A2
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