An alternative method for assaying cerebrospinal fluid protein in the presence of methotrexate.

1988 ◽  
Vol 34 (10) ◽  
pp. 2091-2092 ◽  
Author(s):  
L M Kasper ◽  
W R Moorehead ◽  
T O Oei ◽  
M Markanich
Keyword(s):  
Cerebrospinal Fluid ◽  
Hydrochloric Acid ◽  
Trichloroacetic Acid ◽  
Dilute Hydrochloric Acid ◽  
Constant Amount ◽  
Manual Method ◽  
Turbidimetric Method ◽  
Methotrexate Concentration ◽  
Cerebrospinal Fluid Protein ◽  
Positive Interference

Abstract Therapeutic concentrations of methotrexate can cause significant positive interference in cerebrospinal fluid (CSF) protein values when assayed in the Du Pont aca. Conversely, our modified turbidimetric method, in which trichloroacetic acid (TCA) plus a sample blank containing dilute hydrochloric acid is used in place of TCA, exhibits little or no interference from methotrexate. This was verified by assaying solutions that contained a constant amount of protein (approximately 430 mg/L) and various amounts of methotrexate (0.0-2.3 x 10(-4) mol/L) by both the Du Pont aca and the manual turbidimetric method. As expected, the aca results showed increasing protein values with increasing methotrexate, whereas the manual method gave results approximating the expected protein value irrespective of the methotrexate concentration.

Influence of methotrexate on results by three methods for determining protein in cerebrospinal fluid.

1977 ◽  
Vol 23 (9) ◽  
pp. 1773-1774 ◽  
Author(s):  
M H Zweig
Keyword(s):  
Cerebrospinal Fluid ◽  
Lowry Method ◽  
High Concentrations ◽  
Cerebrospinal Fluid Protein ◽  
Positive Interference

Abstract Because high intravenous and intrathecal doses of methotrexate are used in treatment for various neoplasms, this drug can reach high concentrations in cerebrospinal fluid. At those encountered clinically, it produced significant positive interference with values for cerebrospinal fluid protein as determined with the du Pont aca. A small interference was found with the Folin-Lowry method, and virtually no interference with an automated immunochemical procedure.

Critique of the Determination of Proteins in Cerebrospinal Fluid

1962 ◽  
Vol 8 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Eugene W Rice ◽  
John W Loftis
Keyword(s):  
Cerebrospinal Fluid ◽  
Reference Method ◽  
Turbidimetric Method ◽  
Cerebrospinal Fluid Protein

Abstract Cerebrospinal fluid protein values obtained by the biuret procedure of Goa or the TCA-turbidimetric method of Meulemans are shown to be comparable to those obtained by the more involved tyrosine-equivalence "reference" method of Johnston and Gibson. Both of the former methods are recommended for routine use because of their accuracy and simplicity.

Cerebrospinal fluid protein findings during prophylaxis and treatment of neuromeningosis in leukaemic patients

1987 ◽  
Vol 47 (8) ◽  
pp. 765-769
Author(s):  
R. Marra ◽  
L. Pagano ◽  
S. Storti ◽  
A. Massaro ◽  
L. Teofili ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Cerebrospinal Fluid Protein

Kinetics of cyclization of S-ethoxycarbonylmethylisothiouronium chloride to 2-imino-4-thiazolidone

1979 ◽  
Vol 44 (3) ◽  
pp. 912-917 ◽  
Author(s):  
Vladimír Macháček ◽  
Said A. El-bahai ◽  
Vojeslav Štěrba
Keyword(s):  
Hydrochloric Acid ◽  
Dilute Hydrochloric Acid ◽  
Base Catalysis ◽  
General Base ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Kinetics Of Formation ◽  
Acid Catalyzed ◽  
Rate Limiting ◽  
Kinetics Of

Kinetics of formation of 2-imino-4-thiazolidone from S-ethoxycarbonylmethylisothiouronium chloride has been studied in aqueous buffers and dilute hydrochloric acid. The reaction is subject to general base catalysis, the β value being 0.65. Its rate limiting step consists in acid-catalyzed splitting off of ethoxide ion from dipolar tetrahedral intermediate. At pH < 2 formation of this intermediate becomes rate-limiting; rate constant of its formation is 2 . 104 s-1.

Synthesis of the semi-rigid analogues of prothiadene and dithiadene as potential antidepressant and antihistamine agents: 11-[2-(Dimethylaminomethyl)cyclohexylidene]-6,11-dihydrodibenzo[b,e]thiepins and 4-[2-(dimethylaminomethyl)cyclohexylidene]-4,9-dihydrothieno-[2,3-c]-2-benzothiepins

1985 ◽  
Vol 50 (5) ◽  
pp. 1078-1088 ◽  
Author(s):  
Zdeněk Polívka ◽  
Jiří Holubek ◽  
Emil Svátek ◽  
Jan Metyš ◽  
Miroslav Protiva
Keyword(s):  
Hydrochloric Acid ◽  
Ir Spectrum ◽  
Title Compound ◽  
Dilute Hydrochloric Acid ◽  
Amino Alcohols ◽  
Geometric Isomers ◽  
Pharmacological Testing

Reaction of dibenzo[b,e]thiepin-11(6H)-one with 2-(dimethylaminomethyl)cyclohexylmagnesium chloride gave a mixture of stereoisomeric amino alcohols IX from which four homogeneous bases (IXa to IXd) were separated by chromatography. Dehydration of these compounds with boiling dilute hydrochloric acid afforded mixtures of racemic geometric isomers of the title compound VII, which were separated by crystallization. To the prevailing less polar base VIIa (E)-configuration was assigned on the basis of the IR spectrum. Using a similar procedure, thieno[2,3-c]-2-benzothiepin-4(9H)-one gave mixture of amino alcohols X from which three homogeneous stereoisomers X-A to X-C were isolated. Their dehydration resulted in both expected racemic geometric isomers VIII-A and VIII-B. Pharmacological testing proved the character of an antidepressant for the semi-rigid analogue of dithiadene VIII.

Extremely Elevated Cerebrospinal Fluid Protein and Glucose Level in a Neonate with Hypernatremic Dehydration

2020 ◽  
Author(s):  
Arti Maria ◽  
Tapas Bandyopadhyay
Keyword(s):  
Cerebrospinal Fluid ◽  
Neurodevelopmental Outcome ◽  
Neurological Sequelae ◽  
Glucose Levels ◽  
Csf Analysis ◽  
First Case ◽  
Time Of Admission ◽  
Cerebrospinal Fluid Protein ◽  
Hypernatremic Dehydration

AbstractWe describe the case of a term newborn who presented with hypernatremic dehydration on day 19 of life. The baby was otherwise hemodynamically stable with no evidence of focal or asymmetric neurological signs. The laboratory tests at the time of admission were negative except for hypernatremia and the extremely elevated levels of cerebrospinal fluid (CSF) protein (717 mg/dL) and glucose levels (97 mg/dL). The hypernatremic dehydration was corrected as per the unit protocol over 48 hours. Repeat CSF analysis done after 5 days showed normalization of the protein and glucose levels. Serial follow-up and neuroimaging showed no evidence of neurological sequelae. Unique feature of our case is this is the first case reporting such an extreme elevation of CSF protein and glucose levels that have had no bearing on neurodevelopmental outcome at 1 month and 3 months of follow-up.

Resolution of brain edema in severe brain injury at controlled high and low intracranial pressures

1984 ◽  
Vol 61 (4) ◽  
pp. 707-712 ◽  
Author(s):  
Meihong Cao ◽  
He Lisheng ◽  
Sun Shouzheng
Keyword(s):  
Cerebrospinal Fluid ◽  
Brain Injury ◽  
Brain Edema ◽  
External Ventricular Drainage ◽  
Blue Dye ◽  
Severe Brain Injury ◽  
Content Type ◽  
Intracranial Pressures ◽  
Low Levels ◽  
Cerebrospinal Fluid Protein

✓ A series of 87 patients with severe brain injury were studied. Intracranial pressure (ICP) monitoring and external ventricular drainage were used to control ICP at high and low levels. Clearance of ytterbium-169-labeled diethylenetriaminepentaacetic acid (169Yb-DTPA), Evans blue dye, and ventricular cerebrospinal fluid protein was measured at the two ICP levels over consecutive periods of 4 hours to confirm clearance of brain edema. The results support the hypothesis that brain edema is in part absorbed in the cerebrospinal fluid via transventricular flow.

THE DIENCEPHALIC SYNDROME OF EARLY INFANCY DUE TO SILENT BRAIN TUMOR: WITH SPECIAL REFERENCE TO TREATMENT

PEDIATRICS ◽  
1966 ◽  
Vol 38 (3) ◽  
pp. 473-482
Author(s):  
Harry W. Bain ◽  
John M. M. Darte ◽  
William S. Keith ◽  
Evert Kruyff
Keyword(s):  
Cerebrospinal Fluid ◽  
Brain Tumor ◽  
Food Intake ◽  
Special Reference ◽  
Eosinophil Count ◽  
Clinical Observation ◽  
Stimulation Test ◽  
Diencephalic Syndrome ◽  
Physical Findings ◽  
Cerebrospinal Fluid Protein

Three patients with typical "diencephalic syndrome" have been described. The clinical observation of an emaciated and pale, but remarkably bright, cheerful, and active infant with a normal or increased appetite and food intake should suggest the diagnosis. Physical findings are conspicuous by their absence, although mild nystagmus, tremor, or ataxia may occasionally be present. An elevated total eosinophil count and an elevated cerebrospinal fluid protein are important, though not invariable, features. Metopirone test coupled with ACTH-adrenal stimulation test indicate markedly reduced pituitary reserve. Diagnosis is confirmed by pneumoencephalography and biopsy. It is likely that with increasing knowledge, the clinical spectrum of this syndrome will be broadened, and one might expect to find, on occasions, anorexia rather than increased appetite, lassitude rather than hyperactivity, irritability rather than euphoria, obesity rather than emaciation, and different combinations of all of these features. The results of therapy with beam-directed cobalt irradiation are encouraging.

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