Transferrin Index: An Alternative Method for Calculating the Iron Saturation of Transferrin

1992 ◽  
Vol 38 (10) ◽  
pp. 2078-2081 ◽  
Author(s):  
J Beilby ◽  
J Olynyk ◽  
S Ching ◽  
A Prins ◽  
N Swanson ◽  
...  
Keyword(s):  
Iron Overload ◽  
Serum Iron ◽  
Binding Capacity ◽  
Clinical Chemistry ◽  
Iron Status ◽  
Laboratory Methods ◽  
Control Subjects ◽  
Iron Saturation ◽  
Iron Binding Capacity ◽  
Better Than

Abstract We surveyed 140 clinical chemistry laboratories in Australia to establish which laboratory methods they used to determine serum iron status: 125 measured serum iron (Fe), 85 measured transferrin (TRF), 47 measured total iron-binding capacity (TIBC), and 14 measured both TRF and TIBC. Of the 55 laboratories routinely reporting TRF saturation (TS), 16 calculated TS directly as (Fe/TIBC) x 100, and 9 used [Fe/(TRF x 2)] x 100. Thirty laboratories measured TRF and converted it to an equivalent TIBC concentration; the derived TIBC was then used to calculate TS. We measured iron, TIBC, and TRF concentrations in 94 control subjects, 59 patients with alcoholic liver disease (ALD), and 20 with proven genetic hemochromatosis (GH). TS was compared with a transferrin index (TI = Fe/TRF) to determine whether both methods were sensitive for GH screening and which method gave the fewest false-positive results with discrimination limits of > 55% and > 1.0, respectively. All GH patients were detected by both TS and TI at these limits. One control subject had a TI > 1.0, whereas three control subjects had a TS > 55%. Nine patients with ALD had a TI > 1.0 and 11 ALD patients had a TS > 55%. Some iron-overload patients had lower than expected TS values compared with TI, possibly because of ferritin interference in the TIBC assay. Also, the precision of the TRF assay was better than that of the TIBC assay: CVs of 1.85-3.68% vs 6.17%. We therefore recommend that calculated TI replace TS in screening for iron overload.

scholarly journals Transferrin Polymorphism Influences Iron Status in Blacks

2000 ◽  
Vol 46 (10) ◽  
pp. 1535-1539 ◽  
Author(s):  
Ishmael Kasvosve ◽  
Joris R Delanghe ◽  
Zvenyika A R Gomo ◽  
Innocent T Gangaidzo ◽  
Hlosukwazi Khumalo ◽  
...  
Keyword(s):  
At Risk ◽  
Iron Overload ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Equilibrium Dialysis ◽  
Reference Population ◽  
Functional Difference ◽  
Starch Gel Electrophoresis ◽  
Iron Binding Capacity

Abstract Background: Genetic variants of human transferrin (TF) have been described, but little is known about their functional differences. We studied iron status according to TF phenotype in a healthy Zimbabwean population and in subjects at risk of African iron overload. Methods: The study population consisted of 483 nondrinkers, 31 drinking spouse pairs, and 5 family pedigrees (n = 88) with index cases of iron overload. TF phenotypes were determined using starch gel electrophoresis. To evaluate iron status, serum iron, total iron-binding capacity (TIBC), ferritin, and soluble TF receptors were measured, and the percentage of saturation and the serum iron:TF ratio were calculated. The binding of the TF variants was studied by equilibrium dialysis. Results: The reference population was characterized by a high TF D allele frequency (0.050) and a complete absence of homozygous TF DD individuals. Similar allele frequencies were observed in subjects at risk of African iron overload. In the reference population, male TF CD heterozygotes had significantly lower (P <0.01) values for serum iron, TIBC, TF saturation, and serum iron:TF ratio than the TF CC homozygotes; in females, only TIBC was significantly different. Overall red blood cell indices did not differ according to TF phenotype. In the population at risk of African iron overload, only serum iron:TF ratio was consistently significantly lower in TF CD phenotypes (P <0.05). After equilibrium dialysis, the amount of iron bound by TF was significantly lower (P <0.01) in TF CD individuals. Conclusions: The present data demonstrate a functional difference between TF phenotypes in blacks.

scholarly journals NEOPTERIN AND INTERFERON-GAMMA AS IMMUNE RESPONSE MARKERS IN BETATHALASSEMIA MAJOR PATIENTS

2018 ◽  
Vol 11 (9) ◽  
pp. 192
Author(s):  
Shahad F Obeid ◽  
Sanad B Al-a'araji ◽  
Bassam F Matti ◽  
Hayder Adnan Fawzi
Keyword(s):  
Immune System ◽  
Interferon Gamma ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
System Response ◽  
Iron Binding ◽  
Ifn Γ ◽  
Immune System Response ◽  
Iron Binding Capacity

Objective: The assessment of neopterin and interferon-gamma (IFN-γ) levels as a part of immune system response about serum iron status in β-thalassemia (TM) major patients.Methods: Spectrophotometry applied for the estimation of iron status including serum iron level, total iron-binding capacity, and unsaturated iron-binding capacity. Enzyme-linked immunosorbent assay (ELISA) applied for the estimation of the serum cytokines included neopterin and IFN-γ also serum ferritin estimation by ELISA from 130 β-TM major patients where they divided according to serum ferritin level (< and ≥2500 ng/mL).Results: The neopterin and IFN-γ concentration showed significantly increased with direct correlation among TM patient group when compared to the normal healthy control group. However, there was no significant difference between different levels of serum ferritin.Conclusion: The increased serum level of neopterin and IFN-γ found in patients with β-TM may be due to the direct effect of iron on cellular immune cells beside of immune system response to the effect of iron toxicity on different body organs. There was a direct moderate relationship between neopterin and IFN-γ.

scholarly journals Evaluation of Erythrocytes, Platelets, and Serum Iron Profile in Dogs with Chronic Enteropathy

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Veronica Marchetti ◽  
George Lubas ◽  
Andrea Lombardo ◽  
Michele Corazza ◽  
Grazia Guidi ◽  
...  
Keyword(s):  
Serum Iron ◽  
Activity Index ◽  
Binding Capacity ◽  
Iron Status ◽  
Disease Activity Index ◽  
Microcytic Anemia ◽  
Chronic Enteropathy ◽  
Before And After ◽  
Iron Binding Capacity ◽  
Iron Profile

The aim of this study is to evaluate iron status, erythrocyte, and platelet modifications in dogs with chronic enteropathy (CE). Dogs were grouped as food-responsive diarrhea (FRD, ), antibiotic-responsive diarrhea (ARD, ), and steroid-responsive diarrhea (SRD, ) relating to therapeutic-response. Clinical and haematological findings, evidence of gastrointestinal blood loss, and iron metabolism were evaluated before and after treatment. A mild normocytic or microcytic anemia and thrombocytosis were identified, respectively in 18.0% and 31.8% of CE dogs. No significant differences between pre- and posttreatment of hematocrit, haemoglobin, and mean corpuscular volume, platelet count and mean platelet volume were found. Statistical analysis pointed out significant differences between pre- and posttreatment in serum iron () and unsaturated iron binding capacity (UIBC) (). No significant correlations were found between these parameters and canine Inflammatory Bowel Disease activity index and pattern of CE as well.

Day-to-day variation in serum ferritin concentration in healthy subjects.

1981 ◽  
Vol 27 (1) ◽  
pp. 78-82 ◽  
Author(s):  
V A Pilon ◽  
P J Howanitz ◽  
J H Howanitz ◽  
N Domres
Keyword(s):  
Serum Ferritin ◽  
Healthy Subjects ◽  
Serum Iron ◽  
Binding Capacity ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Ferritin Concentration ◽  
Iron Saturation ◽  
Iron Binding Capacity

Abstract We report our observations on day-to-day variation in serum ferritin, serum iron, total iron-binding capacity, and percent saturation of binding proteins with iron in 13 ostensibly healthy subjects during five weeks. The average intrasubject coefficients of variation were 14.5, 28.5, 4.8, and 28.0%, respectively. Precision studies on control samples showed greater within-assay and between-assay analytical variation for serum ferritin than for serum iron or total iron-binding capacity. Evidently, serum ferritin varies less in a given individual from day to day than do serum iron and percent iron saturation. Thus, a single measurement of serum ferritin may be a more reliable index of iron stores than an isolated determination of either serum iron or percent iron saturation.

scholarly journals Study Iron Homeostasis of Infected and Non-Infected Iraqi Camels with Trypanosomiasis

2020 ◽  
Vol 44 (2) ◽  
pp. 35-44
Author(s):  
Laith S.G. Al-Rubaie
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Cutoff Point ◽  
Iron Binding ◽  
Dromedary Camels ◽  
Point Test ◽  
Iron Binding Capacity

Trypanosomiasis is one of the common parasitic diseases, which infects the dromedary camels and decreases the numbers of these animals in Iraq. To get the best knowledge of the changes of iron status in camels infected with trypanosomiasis, in an attempt to take advantage of these variables as markers for infection, we designed this study. The current study conducted in blood sample collection from155 dromedary camels, 33 were infected (21.29%), according to the status of infection with Trypanosoma evansi that depends on blood smear examination as a golden test. Results denote significant differences of infection ratio by sex and age, from total of 132 male tested, 29 (21.96%) infected, distributed into 12 (%41.37) of age ≤ 2 years and 17 (58.62) of ≥ 2 years. From 23 female tested, 4 (17.39%) were infected at age ≤ 2 years. Furthermore, the results of this study demonstrated significant (p˂0.05) decrease in total serum iron, transferrin saturation, ferritin, whereas increased in total iron binding capacity and unsaturated iron binding capacity in the infected male and female camels with different age. Analyzed data of iron status parameters denoted that the cutoff point test between sensitivity (97) and specificity (100) for serum iron is (≤67.26), for transferrin saturation is (≤17.23) between the sensitivity and specificity (100) and (≥378.66), for total iron binding capacity between the sensitivity and specificity (93.9 and 96.7) respectively. Also, the cutoff point test between the sensitivity (100) and specificity (96.7) for unsaturated iron binding capacity is (≥301.27) and ferritin concentration has a cutoff point is (≤ 249.88) for the sensitivity (100) and specificity (99.2). It could be concluded from what was stated in the results of the current study, that the measurement of the concentration of serum ferritin could be considered as a good marker for the T. evansi infection

A commercial electrochemical method evaluated for measurement of iron status.

1987 ◽  
Vol 33 (9) ◽  
pp. 1645-1647 ◽  
Author(s):  
B S Skikne
Keyword(s):  
Electrochemical Method ◽  
Serum Iron ◽  
Environmental Science ◽  
Binding Capacity ◽  
Iron Status ◽  
Magnesium Carbonate ◽  
Panel Method ◽  
Total Iron ◽  
Analytical Recovery ◽  
Iron Binding Capacity

Abstract I evaluated a commercial electrochemical method (Environmental Science Associates), based on potentiostatic coulometry, for determining iron and total iron-binding capacity (TIBC). The method requires 25 and 100 microL of serum, respectively, for these measurements. Serum iron concentrations so determined in 99 normal volunteers satisfactorily correlated with those determined by the Iron Panel Method (Br J Haematol 1978;38:291-4), although the former required correction for lower-than-expected values. The coulometric TIBC results, on the other hand, correlated poorly with those by the Iron Panel Method. However, when the samples for TIBC were prepared according to the Iron Panel Method, by using the magnesium carbonate technique, and then read in the coulometer, there was satisfactory correlation between TIBC values as determined by the two methods. Variability and analytical recovery of the electrochemical method are satisfactory, and the method should be useful for measurement of serum iron when only relatively small volumes of blood are available.

scholarly journals Acute responses of iron indices in Quarter Horses during a 3-barrel racing exercise

2018 ◽  
Vol 87 (2) ◽  
pp. 109-114
Author(s):  
Clarisse S. Coelho ◽  
Mario Cappi Neto ◽  
Marcela B. Binda ◽  
Fernanda A. Teixeira ◽  
Renan S. Carvalho ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Exercise Physiology ◽  
Transferrin Saturation ◽  
Haemoglobin Concentration ◽  
Kolmogorov Smirnov ◽  
Iron Binding Capacity ◽  
Iron Profile

Energy metabolism and physical performance are dependent on sufficient iron metabolism. Therefore, studies evaluating the iron profile are necessary to elucidate this trace mineral requirements and its role in the equine exercise physiology. The aim of this study was to evaluate the influence of barrel racing exercise on serum iron profile of Quarter horses. Twenty-two regularly trained Quarter horses (8 females and 14 males, 4.8 ± 2.4 years old, mean body weight 431.7 ± 33.9 kg) were enrolled in this study with the owner’s informed consent. All horses were evaluated at T0 (at rest), T1 (immediately after barrel racing trial), T2 (30 min after trial) and T3 (2 h after trial). At these time points, blood samples were taken to determine the packed cell volume (PCV), red blood cell count (RBC), haemoglobin concentration (Hb), total protein (TP), serum iron (SI), total iron-binding capacity (TIBC) and transferrin saturation (TSAT). Variables were analyzed for normality through Kolmogorov-Smirnov test and comparisons were made using Tukey test, considering P < 0.05. The imposed exercise challenge significantly altered PCV, RBC, haemoglobin concentration, TP, SI and TSAT, with higher values were recorded at T1. These findings can be linked to an increased demand as a result of physical activity. Significant changes occurred in the iron status in physically well-conditioned Quarter horses during the barrel racing exercise. Better understanding of iron metabolism in horses will help determine the actual necessity of supplementation.

Clinico-pathological studies on naturally-occurring bovine fascioliasis in the Sudan

1975 ◽  
Vol 49 (3) ◽  
pp. 143-152 ◽  
Author(s):  
E. M. Haroun ◽  
M. F. Hussein
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Concentration ◽  
Total Iron ◽  
The Other ◽  
Liver Pathology ◽  
Total Iron Binding Capacity ◽  
Iron Saturation ◽  
Naturally Occurring ◽  
Iron Binding Capacity

AbstractAn investigation was made of the pathological, haematological and biochemical aspects of naturallyoccurring bovine fascioliasis in the Sudan. 228 animals infected with Fasdola gigantica and 25 non-infected controls were used in the study.The infected cattle revealed emaciation, typical liver pathology, and, occasionally, lesions in the lung and the pancreas. Analysis of their sera also showed reduced albumin values, increased globulin concentrations and decreased albumin/globulin ratio, in addition to increased arginase activity. The serum iron concentration, on the other hand, was decreased, while the total iron binding capacity increased and the resultant iron saturation values reduced. Haematological findings in the infected animals included reduced erythrocyte counts, decreased haematocrjt values, increased mean corpuscular volumes, eosinophilia and decreased neutrophil, lymphocyte and monocytc counts.

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