scholarly journals Evaluation of Erythrocytes, Platelets, and Serum Iron Profile in Dogs with Chronic Enteropathy

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Veronica Marchetti ◽  
George Lubas ◽  
Andrea Lombardo ◽  
Michele Corazza ◽  
Grazia Guidi ◽  
...  
Keyword(s):  
Serum Iron ◽  
Activity Index ◽  
Binding Capacity ◽  
Iron Status ◽  
Disease Activity Index ◽  
Microcytic Anemia ◽  
Chronic Enteropathy ◽  
Before And After ◽  
Iron Binding Capacity ◽  
Iron Profile

The aim of this study is to evaluate iron status, erythrocyte, and platelet modifications in dogs with chronic enteropathy (CE). Dogs were grouped as food-responsive diarrhea (FRD, ), antibiotic-responsive diarrhea (ARD, ), and steroid-responsive diarrhea (SRD, ) relating to therapeutic-response. Clinical and haematological findings, evidence of gastrointestinal blood loss, and iron metabolism were evaluated before and after treatment. A mild normocytic or microcytic anemia and thrombocytosis were identified, respectively in 18.0% and 31.8% of CE dogs. No significant differences between pre- and posttreatment of hematocrit, haemoglobin, and mean corpuscular volume, platelet count and mean platelet volume were found. Statistical analysis pointed out significant differences between pre- and posttreatment in serum iron () and unsaturated iron binding capacity (UIBC) (). No significant correlations were found between these parameters and canine Inflammatory Bowel Disease activity index and pattern of CE as well.

scholarly journals Acute responses of iron indices in Quarter Horses during a 3-barrel racing exercise

2018 ◽  
Vol 87 (2) ◽  
pp. 109-114
Author(s):  
Clarisse S. Coelho ◽  
Mario Cappi Neto ◽  
Marcela B. Binda ◽  
Fernanda A. Teixeira ◽  
Renan S. Carvalho ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Exercise Physiology ◽  
Transferrin Saturation ◽  
Haemoglobin Concentration ◽  
Kolmogorov Smirnov ◽  
Iron Binding Capacity ◽  
Iron Profile

Energy metabolism and physical performance are dependent on sufficient iron metabolism. Therefore, studies evaluating the iron profile are necessary to elucidate this trace mineral requirements and its role in the equine exercise physiology. The aim of this study was to evaluate the influence of barrel racing exercise on serum iron profile of Quarter horses. Twenty-two regularly trained Quarter horses (8 females and 14 males, 4.8 ± 2.4 years old, mean body weight 431.7 ± 33.9 kg) were enrolled in this study with the owner’s informed consent. All horses were evaluated at T0 (at rest), T1 (immediately after barrel racing trial), T2 (30 min after trial) and T3 (2 h after trial). At these time points, blood samples were taken to determine the packed cell volume (PCV), red blood cell count (RBC), haemoglobin concentration (Hb), total protein (TP), serum iron (SI), total iron-binding capacity (TIBC) and transferrin saturation (TSAT). Variables were analyzed for normality through Kolmogorov-Smirnov test and comparisons were made using Tukey test, considering P < 0.05. The imposed exercise challenge significantly altered PCV, RBC, haemoglobin concentration, TP, SI and TSAT, with higher values were recorded at T1. These findings can be linked to an increased demand as a result of physical activity. Significant changes occurred in the iron status in physically well-conditioned Quarter horses during the barrel racing exercise. Better understanding of iron metabolism in horses will help determine the actual necessity of supplementation.

scholarly journals NEOPTERIN AND INTERFERON-GAMMA AS IMMUNE RESPONSE MARKERS IN BETATHALASSEMIA MAJOR PATIENTS

2018 ◽  
Vol 11 (9) ◽  
pp. 192
Author(s):  
Shahad F Obeid ◽  
Sanad B Al-a'araji ◽  
Bassam F Matti ◽  
Hayder Adnan Fawzi
Keyword(s):  
Immune System ◽  
Interferon Gamma ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
System Response ◽  
Iron Binding ◽  
Ifn Γ ◽  
Immune System Response ◽  
Iron Binding Capacity

Objective: The assessment of neopterin and interferon-gamma (IFN-γ) levels as a part of immune system response about serum iron status in β-thalassemia (TM) major patients.Methods: Spectrophotometry applied for the estimation of iron status including serum iron level, total iron-binding capacity, and unsaturated iron-binding capacity. Enzyme-linked immunosorbent assay (ELISA) applied for the estimation of the serum cytokines included neopterin and IFN-γ also serum ferritin estimation by ELISA from 130 β-TM major patients where they divided according to serum ferritin level (< and ≥2500 ng/mL).Results: The neopterin and IFN-γ concentration showed significantly increased with direct correlation among TM patient group when compared to the normal healthy control group. However, there was no significant difference between different levels of serum ferritin.Conclusion: The increased serum level of neopterin and IFN-γ found in patients with β-TM may be due to the direct effect of iron on cellular immune cells beside of immune system response to the effect of iron toxicity on different body organs. There was a direct moderate relationship between neopterin and IFN-γ.

scholarly journals Serum Iron Profile in Chronic Renal Failure Patients

2012 ◽  
Vol 2 (2) ◽  
pp. 8-13
Author(s):  
Md Helal Miah ◽  
Md Zakir Hossain ◽  
Bidhu Bhusun Das ◽  
Md Mokhlesur Rahman Sarker ◽  
Sumanta Kumar Saha ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Iron Deficiency ◽  
Renal Insufficiency ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Research Centre ◽  
Iron Deficient ◽  
Iron Profile

Background & objective: Chronic Renal Failure (CRF) is a serious condition with a worldwide impact. Anemia is a common complication of CRF leading to significant morbidity. Iron deficiency may be a contributing factor for developing anemia in CRF patients. It may be particularly problematic during erythropoietin therapy. This study was intended to find the serum iron status in CRF patients. Method: This cross sectional study was conducted on 100 consecutive patients of CRF in the Department of Medicine, Rangpur Medical College & Hospital, Rangpur and Hypertension & Research Centre, Rangpur between July 2010 to June 2012. Result: 40% of the patients were 40-59 years old, 28% were 20 - 39 years and 32% >60 years old. Males were predominant (64%) than the females (36%). None of the biochemical variables (serum creatinine, haemoglobin and serum iron profile), except total iron binding capacity (TIBC) differ by sex. TIBC was significantly higher in females than that males (p = 0.029). Of the 100 patients 40% were iron deficient; of them 12(30%) had absolute iron deficiency and 28(70%) relative iron deficiency. Iron deficiency was not influenced by sex (p = 0.519). It was not even affected by the degree of renal insufficiency (p = 0.524). However, the incidence of severe anemia increases significantly with the degree of renal insufficiency (p = 0.037). Conclusion: Serum iron profile remains normal in most of the cases of CRF patients. It should be investigated in every CRF patients before deciding for iron therapy. Ibrahim Cardiac Med J 2012; 2(2): 8-13

Transferrin Index: An Alternative Method for Calculating the Iron Saturation of Transferrin

1992 ◽  
Vol 38 (10) ◽  
pp. 2078-2081 ◽  
Author(s):  
J Beilby ◽  
J Olynyk ◽  
S Ching ◽  
A Prins ◽  
N Swanson ◽  
...  
Keyword(s):  
Iron Overload ◽  
Serum Iron ◽  
Binding Capacity ◽  
Clinical Chemistry ◽  
Iron Status ◽  
Laboratory Methods ◽  
Control Subjects ◽  
Iron Saturation ◽  
Iron Binding Capacity ◽  
Better Than

Abstract We surveyed 140 clinical chemistry laboratories in Australia to establish which laboratory methods they used to determine serum iron status: 125 measured serum iron (Fe), 85 measured transferrin (TRF), 47 measured total iron-binding capacity (TIBC), and 14 measured both TRF and TIBC. Of the 55 laboratories routinely reporting TRF saturation (TS), 16 calculated TS directly as (Fe/TIBC) x 100, and 9 used [Fe/(TRF x 2)] x 100. Thirty laboratories measured TRF and converted it to an equivalent TIBC concentration; the derived TIBC was then used to calculate TS. We measured iron, TIBC, and TRF concentrations in 94 control subjects, 59 patients with alcoholic liver disease (ALD), and 20 with proven genetic hemochromatosis (GH). TS was compared with a transferrin index (TI = Fe/TRF) to determine whether both methods were sensitive for GH screening and which method gave the fewest false-positive results with discrimination limits of &gt; 55% and &gt; 1.0, respectively. All GH patients were detected by both TS and TI at these limits. One control subject had a TI &gt; 1.0, whereas three control subjects had a TS &gt; 55%. Nine patients with ALD had a TI &gt; 1.0 and 11 ALD patients had a TS &gt; 55%. Some iron-overload patients had lower than expected TS values compared with TI, possibly because of ferritin interference in the TIBC assay. Also, the precision of the TRF assay was better than that of the TIBC assay: CVs of 1.85-3.68% vs 6.17%. We therefore recommend that calculated TI replace TS in screening for iron overload.

scholarly journals Transferrin Polymorphism Influences Iron Status in Blacks

2000 ◽  
Vol 46 (10) ◽  
pp. 1535-1539 ◽  
Author(s):  
Ishmael Kasvosve ◽  
Joris R Delanghe ◽  
Zvenyika A R Gomo ◽  
Innocent T Gangaidzo ◽  
Hlosukwazi Khumalo ◽  
...  
Keyword(s):  
At Risk ◽  
Iron Overload ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Equilibrium Dialysis ◽  
Reference Population ◽  
Functional Difference ◽  
Starch Gel Electrophoresis ◽  
Iron Binding Capacity

Abstract Background: Genetic variants of human transferrin (TF) have been described, but little is known about their functional differences. We studied iron status according to TF phenotype in a healthy Zimbabwean population and in subjects at risk of African iron overload. Methods: The study population consisted of 483 nondrinkers, 31 drinking spouse pairs, and 5 family pedigrees (n = 88) with index cases of iron overload. TF phenotypes were determined using starch gel electrophoresis. To evaluate iron status, serum iron, total iron-binding capacity (TIBC), ferritin, and soluble TF receptors were measured, and the percentage of saturation and the serum iron:TF ratio were calculated. The binding of the TF variants was studied by equilibrium dialysis. Results: The reference population was characterized by a high TF D allele frequency (0.050) and a complete absence of homozygous TF DD individuals. Similar allele frequencies were observed in subjects at risk of African iron overload. In the reference population, male TF CD heterozygotes had significantly lower (P &lt;0.01) values for serum iron, TIBC, TF saturation, and serum iron:TF ratio than the TF CC homozygotes; in females, only TIBC was significantly different. Overall red blood cell indices did not differ according to TF phenotype. In the population at risk of African iron overload, only serum iron:TF ratio was consistently significantly lower in TF CD phenotypes (P &lt;0.05). After equilibrium dialysis, the amount of iron bound by TF was significantly lower (P &lt;0.01) in TF CD individuals. Conclusions: The present data demonstrate a functional difference between TF phenotypes in blacks.

scholarly journals Study Iron Homeostasis of Infected and Non-Infected Iraqi Camels with Trypanosomiasis

2020 ◽  
Vol 44 (2) ◽  
pp. 35-44
Author(s):  
Laith S.G. Al-Rubaie
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Cutoff Point ◽  
Iron Binding ◽  
Dromedary Camels ◽  
Point Test ◽  
Iron Binding Capacity

Trypanosomiasis is one of the common parasitic diseases, which infects the dromedary camels and decreases the numbers of these animals in Iraq. To get the best knowledge of the changes of iron status in camels infected with trypanosomiasis, in an attempt to take advantage of these variables as markers for infection, we designed this study. The current study conducted in blood sample collection from155 dromedary camels, 33 were infected (21.29%), according to the status of infection with Trypanosoma evansi that depends on blood smear examination as a golden test. Results denote significant differences of infection ratio by sex and age, from total of 132 male tested, 29 (21.96%) infected, distributed into 12 (%41.37) of age ≤ 2 years and 17 (58.62) of ≥ 2 years. From 23 female tested, 4 (17.39%) were infected at age ≤ 2 years. Furthermore, the results of this study demonstrated significant (p˂0.05) decrease in total serum iron, transferrin saturation, ferritin, whereas increased in total iron binding capacity and unsaturated iron binding capacity in the infected male and female camels with different age. Analyzed data of iron status parameters denoted that the cutoff point test between sensitivity (97) and specificity (100) for serum iron is (≤67.26), for transferrin saturation is (≤17.23) between the sensitivity and specificity (100) and (≥378.66), for total iron binding capacity between the sensitivity and specificity (93.9 and 96.7) respectively. Also, the cutoff point test between the sensitivity (100) and specificity (96.7) for unsaturated iron binding capacity is (≥301.27) and ferritin concentration has a cutoff point is (≤ 249.88) for the sensitivity (100) and specificity (99.2). It could be concluded from what was stated in the results of the current study, that the measurement of the concentration of serum ferritin could be considered as a good marker for the T. evansi infection

A commercial electrochemical method evaluated for measurement of iron status.

1987 ◽  
Vol 33 (9) ◽  
pp. 1645-1647 ◽  
Author(s):  
B S Skikne
Keyword(s):  
Electrochemical Method ◽  
Serum Iron ◽  
Environmental Science ◽  
Binding Capacity ◽  
Iron Status ◽  
Magnesium Carbonate ◽  
Panel Method ◽  
Total Iron ◽  
Analytical Recovery ◽  
Iron Binding Capacity

Abstract I evaluated a commercial electrochemical method (Environmental Science Associates), based on potentiostatic coulometry, for determining iron and total iron-binding capacity (TIBC). The method requires 25 and 100 microL of serum, respectively, for these measurements. Serum iron concentrations so determined in 99 normal volunteers satisfactorily correlated with those determined by the Iron Panel Method (Br J Haematol 1978;38:291-4), although the former required correction for lower-than-expected values. The coulometric TIBC results, on the other hand, correlated poorly with those by the Iron Panel Method. However, when the samples for TIBC were prepared according to the Iron Panel Method, by using the magnesium carbonate technique, and then read in the coulometer, there was satisfactory correlation between TIBC values as determined by the two methods. Variability and analytical recovery of the electrochemical method are satisfactory, and the method should be useful for measurement of serum iron when only relatively small volumes of blood are available.

scholarly journals Impact of dietary intervention with a functional food supplement to combat anemia - the blood iron metabolic disorder among the coffee plantation laborers

2013 ◽  
Vol 3 (1) ◽  
pp. 21
Author(s):  
Vasantha Esther Rani ◽  
Samuel Paulraj
Keyword(s):  
Functional Food ◽  
Serum Iron ◽  
Dietary Intervention ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Iron Binding ◽  
Coffee Plantation ◽  
Iron Binding Capacity ◽  
Iron Profile ◽  
The Impact

Objective: To assess the nutritional status and other nutrition related problems of the workers. To design, implement and evaluate the impact of dietary intervention for the nutritional metabolic disorder which is directly related to productivity.Background: Indian economy greatly relies on agriculture. Agriculture is set to play a more dynamic role in the economy. The present study focuses on the nutritional status with special reference to the blood iron profile of manual coffee plantation laborers belonging to Kodaikannal, Tamil Nadu, India. The outcome of this study on the dietary intervention, based on nutritional picture of coffee plantation laborers, will have a nationwide application because of the simplified, stable coffee plantation operations throughout India.Method: Experiments were carried out in three phases. In the first phase, the personal background of the laborers was assessed. In the second phase, the nutritional and health status of the laborers, through dietary survey comprising 24 Hour Food Recall record and weighment of cooked food consumed for three consecutive days, clinical and biochemical profile were studied. The serum iron was measured with the total iron binding capacity, from which the transferrin saturation was calculated. In the third phase, dietary intervention was implemented with a nutrient rich nutraceutical food supplement - spirulina incorporated soup. The supplementation was extended for a period of 120 days. The subjects were grouped into control and the experimental group. The control group was given plain soup and the experimental group was administered with soup incorporated with spirulina. The impact of intervention on the biochemical and nutritional profile of the laborers was reassessed in similar working conditions.Results: A deficit of 0.1 mg to 0.3 mg of iron intake per kg body weight in the male and female laborers was observed when compared to their RDA. The clinical pictures reveal that 94 percent suffered from anemia, 56 percent showed anemic signs of spooning of nails (koilonychyia), 36 percent of the subjects had glossitis (mouth ulcer), 16 percent suffered from bleeding gums, 4 percent had cheilosis (ulceration of tongue) and 28 percent had angular stomatitis. The average hemoglobin level was 8.6 ± 1.12g/dl for men and 8.1±1.01g/dl for women which are 28 and 37 percent less than normal respectively. Dietary intervention of 5g of spirulina in 150 ml of soup had significantly improved the blood iron profile. Among the male laborers, 10 percent who suffered from severe anemia with 7.9 g.dl-1 hemoglobin before intervention were shifted to moderate levels of 9.4 g.dl-1, 30 percent who suffered from mild anemia were shifted to normal ones and among 60 percent of male laborers who were moderately anemic, 50 percent reverted to normalcy.Conclusion: In the present study it was observed that the habitual dietary intake showed deficiency in consumption of iron rich foods and the clinical picture revealed the signs and symptoms of anemia The blood iron profile - Hemoglobin (Hb), Serum Iron, Transferrin Saturation, Serum Ferritin, Total Iron Binding Capacity (TIBC) and Unsaturated Iron Binding Capacity (UIBC) were greatly influenced by dietary intervention with the functional food Spirulina incorporated soup The blood hemoglobin content increased by 21 percent i.e from 10.9 to 13.3g.dl-1. Serum iron, serum ferritin and transferrin levels showed a consistent and progressive rise while TIBC and UIBC reduced resulting in a positive iron balance. Keywords: Dietary intervention, functional food, Spirulina, hemoglobin, anemia

scholarly journals Relationship of Acute ST-Elevation Myocardial Infarction with hs-CRP and Serum Iron Profile in Southern India: A Cross-sectional Study

2020 ◽  
Author(s):  
MK Malthesh ◽  
Siddharth Gosavi ◽  
Shashank Shastry ◽  
Ramyasri Rajesh ◽  
Pratyaksh P Vaishnav ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Hospital Stay ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Binding ◽  
Cross Sectional ◽  
Hs Crp ◽  
Iron Binding Capacity ◽  
Iron Profile ◽  
Relationship Of

Introduction: Cardiovascular diseases are a major cause of mortality. The role of Iron in oxidative myocardial damage remains controversial with multiple studies showing positive and negative correlations. Systemic inflammation is also an important factor in Myocardial Infarction (MI) with high sensitive C-Reactive Protein (hs-CRP) being an important marker. Aim: To investigate the relationship of serum iron and hs-CRP in patients diagnosed with ST-segment Elevation Myocardial Infarction (STEMI) and its role as prognostic indicators. Materials and Methods: A cross-sectional hospital-based study was conducted in which 45 patients were enrolled over a period of two months. Primary variables studied were STEMI and site of infarction as confirmed on echocardiography, hospital stay and patient outcome. Secondary variables were serum iron, hs-CRP, Total Iron Binding Capacity (TIBC), ferritin. Statistical analysis was done using IBM Statistical Package for the Social Sciences (SPSS) software version 20. Mann-Whitney U test, Kruskal Wallis test, ANOVA and Spearman’s rank correlation was used. Results: Iron profile was significantly altered in the various types of MI. Serum iron values lower than 61 mg/dL had a sensitivity of 89% and high Negative Predictive Value (NPV) (95%) for the prediction of mortality in patients. It was seen that significantly lower Unbound Iron Binding Capacity (UIBC) values were seen in patients who died than the survivor group. Low Transferrin Saturation (TS), serum iron, TIBC were associated with a longer hospital stay. About 42 patients showed hs-CRP levels above 0.3 mg/L. High hs-CRP and ferritin values were associated with a prolonged hospital stay. Conclusion: In patients with acute STEMI, serum iron and hs-CRP are shown to be important predictors of morbidity and mortality. Regular iron supplementation with a six monthly hs-CRP monitoring is recommended. Further research shows screening capability is needed.

Sign in / Sign up

Export Citation Format

Share Document