Determining Total Protein Content of Urine: Centrifugation Results in Precipitation of the Protein-Coomassie Brilliant Blue Dye Complex

ABSTRACTThe protein content of fresh homogenates and their corresponding TCA precipitated fractions of 10 different species of helminths was estimated by the methods of Lowry et al. and Spector using the Folin phenol reagent and Coomassie brilliant blue G-250 respectively. The former method gives exaggerated values as compared to the latter method. The parasite phenols, phenolic proteins and catecholamines could be responsible for interference in the Lowry's procedure. The TCA non-precipitable moieties also give colour only with the Folin phenol assay. The pronounced intra-specific differences in the total protein content of helminths reflect their metabolic variations and adaptations. Habitat does not appear to influence the protein content of parasites, however, the effect of host variation was evident in the pouched amphistome G. crumenifer. It is concluded that the dye binding method gives more consistent results and it can be conveniently applied to crude tissue homogenates of helminths.

Download Full-text

Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.

Clinical Chemistry ◽

10.1093/clinchem/35.10.2127 ◽

1989 ◽

Vol 35 (10) ◽

pp. 2127-2129 ◽

Cited By ~ 14

Author(s):

Y Bergqvist ◽

L Karlsson ◽

L Fohlin

Keyword(s):

Breast Milk ◽

Total Protein ◽

Brilliant Blue ◽

Human Breast Milk ◽

Human Breast ◽

Simple Method ◽

Coomassie Brilliant Blue ◽

Kjeldahl Method ◽

Coomassie Brilliant

Abstract This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.

Download Full-text

Photocatalysis of Coomassie Brilliant Blue Using Clay Mineral

Materials Science Forum ◽

10.4028/www.scientific.net/msf.869.765 ◽

2016 ◽

Vol 869 ◽

pp. 765-767 ◽

Cited By ~ 3

Author(s):

Layane Rodrigues Almeida ◽

João Sammy Nery Souza ◽

Edson Cavalcanti Silva Filho ◽

Josy Anteveli Osajima

Keyword(s):

Organic Pollutants ◽

Uv Light ◽

Brilliant Blue ◽

Blue Dye ◽

Secondary Treatment ◽

Toxic Compounds ◽

Coomassie Brilliant Blue ◽

Kinetics Of ◽

Coomassie Brilliant ◽

Photocatalytic Processes

The presence of organic pollutants, which cannot be eliminated by conventional processes of primary and secondary treatment, can be problematic. Photocatalytic processes offer an efficient breakdown of organic pollutants into non-toxic compounds such as CO2 and H2O. This paper proposes the use of the titanium dioxide embedded in palygorskite as a photoactive material in the degradation of cationic dye, Coomassie Brilliant Blue. The system was irradiated using UV light for a maximum time of 120 minutes. The concentration of the dye used was 1.0x10-4 mol L-1 in 0.5 g L-1 of the photoactive material. The kinetics of the system was monitored by UV-Vis spectrophotometry. In 120 minutes of radiation, the process of photocatalysis reduced the initial concentration of the Coomassie Brilliant Blue dye in half.

Download Full-text