Comparison of Radical Scavenging Activity, Cytotoxic Effects and Apoptosis Induction in Human Melanoma Cells by Taiwanese Propolis from Different Sources

Propolis is a sticky substance that is collected from plants by honeybees. We previously demonstrated that propolins A, B, C, D, E and F, isolated from Taiwanese propolis (TP), could effectively induce human melanoma cell apoptosis and were strong antioxidant agents. In this study, we evaluated TP for free radical scavenging activity by DPPH (1,2-diphenyl-2-picrylhydrazyl). The phenolic concentrations were quantified by the Folin–Ciocalteu method. The apoptosis trigger activity in human melanoma cells was evaluated. TP contained a higher level of phenolic compounds and showed strong capability to scavenge free radicals. Additionally, TP1g, TP3, TP4 and TP7 exhibited a cytotoxic effect on human melanoma cells, with an IC50 of 2.3, 2.0, 3.3 and 3.3 μg/ml, respectively. Flow cytometric analysis for DNA fragmentation indicated that TP1g, TP2, TP3 and TP7 could induce apoptosis in human melanoma cells and there is a marked loss of cells from the G2/M phase of the cell cycle. To address the mechanism of the apoptosis effect of TP, we evaluated its effects on induction of apoptosis-related proteins in human melanoma cells. The levels of procaspase-3 and PARP [poly(ADP-ribose) polymerase] were markedly decreased. Furthermore, propolins A, B, C, D, E and F in TP were determined using HPLC. The results indicate that TP is a rich source of these compounds. The findings suggest that TP induces apoptosis in human melanoma cells due to its high level of propolins.

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Green Synthesis, Characterization, DPPH and Ferrous Ion-chelating (FIC) Activity of Tetrakis-Schiff’s Bases of Terephthalaldehyde

Letters in Drug Design & Discovery ◽

10.2174/1570180815666180531101404 ◽

2019 ◽

Vol 16 (3) ◽

pp. 249-255

Author(s):

Momin Khan ◽

Umar Ali ◽

Anis Ur Rahman ◽

Muhammad Ibrahim ◽

Abdul Hameed ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Aromatic Aldehydes ◽

Ferrous Ion ◽

Future Research ◽

Scavenging Activity ◽

Schiff’S Bases ◽

Schiff's Bases ◽

Antioxidant Agents

Background: The role of small molecules as antioxidants to prevent the oxidation of other molecules and inhibit them from radical formation is the area of much interest to cure disease especially cancer. Moreover, the antioxidants play important role as stabilizers to prevent oxidation of fuels and lubricants. Methods: In the present study, fifteen tetrakis-Schiff’s bases derivatives (1-15) were synthesized and screened for their antioxidant activities. Compounds 1-15 were synthesized by continuous stirring of reaction mixture of 1,4-bis (hydrazonomethyl)benzene (1 mmol) with various substituted aromatic aldehydes (2 mmol) in distilled water using acetic acid as catalyst at room temperature for 2-10 min. Results: Our present study showed that all compounds are better ferrous ion-chelating agents except compound 1,4-bis((E)-((E)-(3,4-dimethoxybenzylidene)hydrazono)methyl)benzene (6) (IC50 = 329.26 ± 4.75 µM) which has slightly low activity than the standard EDTA (IC50 = 318.40 ± 5.53 μM). In addition, DPPH radical scavenging activity of eleven compounds showed higher activity than the standard. However, remaining four compounds showed comparable radical scavenging activity to the standard DPPH (IC50 = 257.77 ± 4.60 µM). Conclusion: The series of fifteen Schiff’s bases (1-15) were synthesized and evaluated as antioxidants. From both assays, it has been demonstrated that most of the tetrakis-Schiff’s bases have potential to serve as leads for the development of antioxidant agents for future research.

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Non-Phenolic Antioxidant Compounds from Buddleja asiatica

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-7-803 ◽

2008 ◽

Vol 63 (7-8) ◽

pp. 483-491 ◽

Cited By ~ 7

Author(s):

Mortada M. El-Sayed ◽

El-Sayed S. Abdel-Hameed ◽

Wafaa S. Ahmed ◽

Eman A. El-Wakil

Keyword(s):

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antioxidant Compounds ◽

Scavenging Activity ◽

Chemical Analyses ◽

Dpph Assay ◽

Antioxidant Agents ◽

Total Antioxidant

The methanol extract of the leaves of Buddleja asiatica Lour. (Loganiaceae) showed antioxidant activity toward the well known in vitro antioxidant tests such as total antioxidant capacity by the phosphomolybdenum method, free radical scavenging activity by the 1,1- diphenyl-2-picrylhydrazyl scavenging assay (DPPH assay) and hydrogen peroxide scavenging methods. Due to the high scavenging activity of the n-butanol successive fraction toward DPPH and H2O2 (SC50 = 11.99 and 18.54 μg/ml, respectively), this extract was subjected to chromatographic separation and isolation. Four non-phenolic compounds were isolated and identified on the basis of spectroscopic and chemical analyses: 1-O-ß-D-glucopyranosyl- 2-methoxy-3-(2-hydroxy-triaconta-3,12-dienoate)-glycerol (1), 3-O-[α-l-rhamnopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-glucopyranosyl-(1→2)]-ß-d-fucopyranosyl-olean- 11,13(18)-diene-3ß,23,28-triol (2), 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-ß-d-fucopyranosyl-olean-11,13(18)-diene-3ß,23,28-triol (3), and 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-xylopyranosyl- (1→2)]-ß-d-glucuronopyranosyl-acid-olean-11,13(18)-diene-3ß,23,28-triol (4). The four compounds were evaluated as antioxidant agents using the three antioxidant bioassay tests.

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STUDY ON Hibiscus tiliaceus LEAVES AS ANTIBACTERIAL AND ANTIOXIDANT AGENTS

Alotrop ◽

10.33369/atp.v3i2.9874 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

Mohamad Syahmie Samsudin ◽

Yosie Andriani ◽

Purbowatiningrum Ria Sarjono ◽

Desy Fitrya Syamsumir

Keyword(s):

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Inhibition Zone ◽

Klebsiella Pneumonia ◽

Scavenging Activity ◽

Cancer Disease ◽

Antioxidant Agents ◽

Dpph Method ◽

Positive Results

This study aimed to evaluate antioxidant and antibacterial activities of methanol, hexane, dichloromethane, ethyl acetate, and water crude sample fractions of H. tiliaceus leaves. Various type of samples play an important role to determining which compound that will give the positive results in antioxidant test (inhibiting free radical scavenging activity) which they probably have potency for preventing the cancer disease. Furthermore, those types of samples were also tested to the antibacterial test as their possibilities to be created as new antibiotics in the future. This research also focused on what compound groups that actually present in H. tiliaceus leaves by using the phytochemistry test. Antioxidant potency of H. tiliaceus leaves extracts for the radical scavenging activity for quantitative assay was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antibacterial potency was measured by the Muller Hinton Agar (MHA) by applied the sample chemical crude in the plate. The result showed that all chemical crude of H. tiliaceus leaves has high antioxidant property as methanol crude (MC) 308.416%; hexane crude (HC) 232.837%; dichloromethane crude (DC) 150.837%; ethyl acetate crude (EC) 73.623% and water crude (WC) 71.777% with respected to the readings of 100% from quercetin (Q) as a positive control. From antibacterial result, entire samples had shown the positive results towards both gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). The ranges of the inhibition zone were both at 7-10 mm and 10-15 mm. The phytochemistry test determine that the samples actually contained a lot of compounds which were proteins, carbohydrates, phenols/tannins, flavonoid, terpenoids, glycosides and also steroid. It is clearly indicated that H. tiliaceus leaves have potential to be used against the antioxidant and also as new antibacterial agents

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Free radical scavenging activity of five selected sea cucumbers collected from Lampung waters, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/890/1/012014 ◽

2021 ◽

Vol 890 (1) ◽

pp. 012014

Author(s):

A. Rasyid ◽

M Y Putra ◽

Yasman

Keyword(s):

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Eastern Region ◽

Scavenging Activity ◽

Sea Cucumbers ◽

Tropical Waters ◽

Antioxidant Agents

Abstract Sea cucumber is an important raw material for food and remedy in the Eastern region. However, a study focusing on identifying the health benefit of sea cucumbers from tropical waters is still limited. A study on the free radical scavenging activity of five selected sea cucumbers collected from Lampung waters, Indonesia has been done. The objective of this study was to evaluate the free radical scavenging activity of five selected sea cucumbers, namely Stichopus vastus, Stichopus quadrifasciatus, Holothuria (Metriatyla) lessoni, Holothuria (Mertensiothuria) leucospilota, and Bohadschia marmorata. The extraction method which used in this study was the maceration method using methanol solvent while the free radical scavenging activity test is carried out using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. Compared to other species at a concentration of 250 µg/mL, S. vastus, S. quadrifasciatus, H. lessoni and H. leucospilota showed the strongest scavenging activity. While B. marmorata was classified as a very weak scavenging activity. It can be concluded that S. vastus, S. quadrifasciatus, H. lessoni H. leucospilota, and H. lessoni are important sea cucumbers from tropical waters as a source of natural antioxidant agents in the future.

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Flow Cytometric Analysis of Fluorescein-Labeled Nerve Growth Factor Binding to A875 Human Melanoma Cells

Experimental Cell Research ◽

10.1006/excr.1994.1012 ◽

1994 ◽

Vol 210 (1) ◽

pp. 77-85 ◽

Cited By ~ 3

Author(s):

Marion T. Kasaian ◽

James W. Jacobberger ◽

Kenneth E. Neet

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Flow Cytometric Analysis ◽

Melanoma Cells ◽

Human Melanoma ◽

Nerve Growth ◽

Factor Binding ◽

Flow Cytometric ◽

Human Melanoma Cells

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Olive Oil Dregs as a Novel Source of Natural Antioxidants: Extraction Optimization towards a Sustainable Process

Processes ◽

10.3390/pr9061064 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1064

Author(s):

Giuseppe Squillaci ◽

Alice Marchetti ◽

Orsolina Petillo ◽

Michela Bosetti ◽

Francesco La Cara ◽

...

Keyword(s):

Olive Oil ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Scavenging Activity ◽

Antioxidant Power ◽

Antioxidant Agents ◽

Ferric Reducing Antioxidant Power

Olive oil dregs (OOD), which are an underutilized by-product from oil mills, were used for the extraction of antioxidant compounds. The residues from three oil mills located in Campania (Southern Italy) were extracted with acidified methanol, and hydroxytyrosol (HT) was the main phenolic compound detected. Total phenolic content (TPC) and HT amount were measured. EVO Campania oil mill provided the residue with the highest TPC and HT quantities: 6.801 ± 0.159 mg Gallic Acid Equivalents (GAE)/g OOD and 519.865 ± 9.082 μg/g OOD, respectively. Eco-friendly extractions at different temperatures and times were performed on EVO Campania OOD, obtaining 9.122 ± 0.104 mg GAE/g OOD and 541.330 ± 64.087 μg/g OOD for TPC and HT, respectively, at 121 °C for 60 min. Radical Scavenging Activity (RSA), Superoxide Scavenging Activity (SSA), and Ferric Reducing Antioxidant Power (FRAP) were measured in OOD aqueous extracts. Extract prepared at 37 °C for 60 min showed the greatest RSA and SSA values (44.12 ± 1.82 and 75.72 ± 1.78, respectively), whereas extract prepared at 121 °C for 60 min exhibited the highest FRAP value (129.10 ± 10.49 μg Ascorbic Acid Equivalents (AAE)/mg). OOD extracts were able to protect sunflower oil from oxidation for 4 weeks at 65 °C. The overall results suggest that this novel residue can be usefully valorized by providing HT-rich extracts to use as antioxidant agents.

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