Wolbachia Infection in Two Species: Novel Views on the Colonization Ability of Wolbachia in Aphids

Abstract Wolbachia pipientis (Rickettsiales: Anaplasmataceae) is an intracellular symbiont residing in arthropods and filarial nematodes. Sixteen supergroups have been described from different host taxa. Four supergroups A, B, M, and N were found in aphids according to prior studies. The cotton aphid, Aphis gossypii, and the green peach aphid, Myzus persicae, are typical polyphagous species with global distributions. We conducted an extensive and systematic survey of Wolbachia infections in these aphids from China. High incidences of Wolbachia infection were detected. The total infection incidence was 60% in A. gossypii and 88% in M. persicae. Both aphid species were infected with supergroups A, B and M. Different incidences of infection were observed among the seven geographical regions in China, which suggested a positive relationship between Wolbachia infections and the geographical distribution of aphid species. Furthermore, multiple infection patterns (M, B, A&M, B&M, and A&B&M) were observed. Infection patterns M and B&M were detected in almost all populations. Patterns A&B&M and B showed geographical restriction in North China. Three factors can possibly influence the Wolbachia infection incidences and patterns: the geographical distribution, aphid species, and different supergroup types.

Download Full-text

Effect of Host Plant, Aphid Species, and Virus Infection Status on Transmission of Sweetpotato feathery mottle virus

Plant Disease ◽

10.1094/pdis-11-11-0934-re ◽

2012 ◽

Vol 96 (9) ◽

pp. 1331-1336 ◽

Cited By ~ 10

Author(s):

E. N. Wosula ◽

C. A. Clark ◽

J. A. Davis

Keyword(s):

Mixed Infection ◽

Ipomoea Batatas ◽

Green Peach Aphid ◽

Aphis Gossypii ◽

Virus Titer ◽

Aphid Species ◽

Morning Glory ◽

Mottle Virus ◽

Infection Status ◽

Cotton Aphid

Sweetpotato feathery mottle virus (SPFMV) is a nonpersistently transmitted virus known to infect sweetpotato (Ipomoea batatas) and wild morning glory plants. SPFMV is vectored by various aphid species, among them the green peach aphid, Myzus persicae, and the cotton aphid, Aphis gossypii. Our objective was to determine whether differences in acquisition hosts (sweetpotato and morning glory), aphid species (M. persicae and A. gossypii), and infection status (single versus mixed infection) influenced transmission of SPFMV. SPFMV transmission from I. hederacea with a natural mixed infection by A. gossypii (39%) was significantly greater than in other host–virus combinations. Successful transmissions by A. gossypii were significantly greater compared with M. persicae in all host–virus combinations. Virus titers in source leaves were significantly greater in single- and mixed-infected I. hederacea and single-infected I. cordatotriloba compared with other host–virus combinations. There was a significant positive correlation between virus titer and transmission by both aphid species. These results suggest that, under controlled conditions, SPFMV is more readily transmitted from infected morning glory plants than from sweetpotato. Additionally, mixed-infected plants are better virus sources for transmission than single-infected, and A. gossypii is a more efficient vector than M. persicae under laboratory conditions.

Download Full-text

Aphis gossypii Glover, melon/cotton aphid, Aulacorthum solani (Kaltenbach), foxglove aphid, Macrosiphum euphorbiae (Thomas), potato aphid, and Myzus persicae (Sulzer), green peach aphid (Homoptera: Aphididae).

Biological Control Programmes in Canada, 1981-2000 ◽

10.1079/9780851995274.0044 ◽

2009 ◽

pp. 44-49

Author(s):

D. R. Gillespie ◽

J. L. Shipp ◽

D. A. Raworth ◽

R. G. Foottit

Keyword(s):

Myzus Persicae ◽

Green Peach Aphid ◽

Aphis Gossypii ◽

Macrosiphum Euphorbiae ◽

Cotton Aphid ◽

Potato Aphid ◽

Aphis Gossypii Glover ◽

Aulacorthum Solani ◽

Foxglove Aphid

Download Full-text

The Bacterial Flora Associated with the Polyphagous Aphid Aphis gossypii Glover (Hemiptera: Aphididae) Is Strongly Affected by Host Plants

Microbial Ecology ◽

10.1007/s00248-019-01435-2 ◽

2019 ◽

Vol 79 (4) ◽

pp. 971-984 ◽

Cited By ~ 3

Author(s):

Shifen Xu ◽

Liyun Jiang ◽

Gexia Qiao ◽

Jing Chen

Keyword(s):

Host Plant ◽

Host Plants ◽

Statistical Tests ◽

Bacterial Flora ◽

Aphis Gossypii ◽

Natural Populations ◽

Aphid Species ◽

Ecological Specialization ◽

Cotton Aphid ◽

Symbiotic Associations

AbstractAphids live in symbiosis with a variety of bacteria, including the obligate symbiont Buchnera aphidicola and diverse facultative symbionts. The symbiotic associations for one aphid species, especially for polyphagous species, often differ across populations. In the present study, by using high-throughput 16S rRNA sequencing, we surveyed in detail the microbiota in natural populations of the cotton aphid Aphis gossypii in China and assessed differences in bacterial diversity with respect to host plant and geography. The microbial community of A. gossypii was dominated by a few heritable symbionts. Arsenophonus was the most dominant secondary symbiont, and Spiroplasma was detected for the first time. Statistical tests and ordination analyses showed that host plants rather than geography seemed to have shaped the associated symbiont composition. Special symbiont communities inhabited the Cucurbitaceae-feeding populations, which supported the ecological specialization of A. gossypii on cucurbits from the viewpoint of symbiotic bacteria. Correlation analysis suggested antagonistic interactions between Buchnera and coexisting secondary symbionts and more complicated interactions between different secondary symbionts. Our findings lend further support to an important role of the host plant in structuring symbiont communities of polyphagous aphids and will improve our understanding of the interactions among phytophagous insects, symbionts, and environments.

Download Full-text

Susceptibility of standard clones and European field populations of the green peach aphid, Myzus persicae, and the cotton aphid, Aphis gossypii (Hemiptera: Aphididae), to the novel anthranilic diamide insecticide cyantraniliprole

Pest Management Science ◽

10.1002/ps.2306 ◽

2011 ◽

Vol 68 (4) ◽

pp. 629-633 ◽

Cited By ~ 43

Author(s):

Stephen P Foster ◽

Ian Denholm ◽

Jean-Luc Rison ◽

Hector E Portillo ◽

John Margaritopoulis ◽

...

Keyword(s):

Myzus Persicae ◽

Green Peach Aphid ◽

Aphis Gossypii ◽

The Novel ◽

Cotton Aphid ◽

Anthranilic Diamide ◽

Diamide Insecticide

Download Full-text

Innovative Approach for the Use of Huwa-San TR50 in Controlling Cotton Aphids (Aphis gossypii Glover)

Journal of Agricultural Science ◽

10.5539/jas.v9n4p77 ◽

2017 ◽

Vol 9 (4) ◽

pp. 77

Author(s):

Saleh S. Alhewairini

Keyword(s):

Host Plants ◽

Aphis Gossypii ◽

Coccinella Septempunctata ◽

Aphid Species ◽

Observable Effect ◽

Cotton Aphid ◽

Main Method ◽

Significant Difference ◽

Aphis Gossypii Glover ◽

And Behavior

Chemical control remains the main method of controlling the cotton aphid (Aphis gossypii Glover). Millions of dollars have been lost due to plant damage which resulted in reduced quality and yield of cotton. Nevertheless, A. gossypii can rapidly develop resistance to different groups of insecticides such as organophosphates, carbamates and pyrethroids. The potential of Huwa-San TR50 in controlling A. gossypii is yet to be tested. Huwa-San TR50 is a formula of hydrogen peroxide which has been stabilized by the addition of a small quantity of silver and has extensively used as a disinfectant. In this study, it was found to be very potent in killing A. gossypii and produced 93.5, 96.5, 97 and 95.5% mortality at 1000, 2000, 3000 and 4000 ppm, respectively, after 48 h of exposure. Furthermore, there was no significant difference between four Huwa-San TR50 concentrations after 48 h of exposure, on the mortality of A. gossypii. Huwa-San TR50 of up to 4000 ppm had no observable effects on the mortality and behavior of adult honeybee workers (Apis mellifera lamarckii) as compared with the control. Also, Huwa-San TR50 concentration of up to 3000 ppm had no observable effect on seven-spot ladybird beetles (Coccinella septempunctata) whereas a concentration of 4000 ppm produced 100% mortality after 24 h of exposure. Huwa-San TR50 concentrations of up to 2000 ppm failed to produce any symptoms on cucumber leaves. The differential effects of Huwa-San TR50 on aphids and beneficial insects, suggest the need for further investigation to understand the effects of Huwa-San TR50 on other host plants of aphids and aphid species.

Download Full-text

The influence of natural enemies on wing induction in Aphis fabae and Megoura viciae (Hemiptera: Aphididae)

Bulletin of Entomological Research ◽

10.1017/s0007485307005391 ◽

2007 ◽

Vol 98 (1) ◽

pp. 59-62 ◽

Cited By ~ 12

Author(s):

G. Kunert ◽

K. Schmoock-Ortlepp ◽

U. Reissmann ◽

S. Creutzburg ◽

W.W. Weisser

Keyword(s):

Natural Enemies ◽

Acyrthosiphon Pisum ◽

Aphis Gossypii ◽

Aphid Species ◽

Pea Aphid ◽

Aphis Fabae ◽

Chrysoperla Carnea ◽

Cotton Aphid ◽

Aphid Density ◽

Megoura Viciae

AbstractPrevious studies have shown that the aphid species, Aphis fabae Scopoli and Megoura viciae Buckton, do not produce winged offspring in the presence of natural enemies, in contrast to results for the pea aphid (Acyrthosiphon pisum (Harris)) and the cotton aphid (Aphis gossypii Glover); but these studies did not involve exposing aphids directly to natural enemies. We exposed colonies of both A. fabae and M. viciae to foraging lacewing (Chrysoperla carnea (Stephens)) larvae and found that the predators did not induce winged morphs among offspring compared to unexposed controls. Colonies of A. fabae responded to an increase in aphid density with increasing winged morph production, while such response was not found for M. viciae. We suggest that different aphid species differ in their susceptibility to natural enemy attack, as well as in their sensitivity to contact.

Download Full-text

Insights into wing dimorphism in the worldwide agricultural pest Aphis gossypii, the host-alternating aphid

10.21203/rs.2.24662/v1 ◽

2020 ◽

Author(s):

Jichao Ji ◽

Shuai Zhang ◽

Ningbo Huangfu ◽

Junyu Luo ◽

Lin Niu ◽

...

Keyword(s):

Signaling Pathways ◽

Molecular Mechanisms ◽

Fatty Acid Biosynthesis ◽

Aphis Gossypii ◽

Pairwise Comparison ◽

Aphid Species ◽

Future Research ◽

Wing Dimorphism ◽

Cotton Aphid ◽

Parthenogenetic Female

Abstract Background: Three wing morphs exists in the life cycle of the worldwide pest Aphis gossypii, i.e., wing parthenogenetic female (WPF), gynopara (GP) and male, which were produced mostly by crowding and host quality, photoperiod, loss of X chromosome, respectively. However, the shared molecular mechanism underlying their wing differentiation remains an enigma. Here we firstly induced gynoparae and males indoors and compared the characters of these wing morphs in body, internal genitals and fecundity. Then we identified the shared and separate differentially expressed genes (DEGs) and signaling pathways potentially involved in the wing morphs regulation in WPF, GP and male compared to wingless parthenogenetic female (WLPF). Results: Newly-born nymphs reared in short photoperiod condition exclusively produce gynoparae and males in adulthood successively, in which the sex ratio is gynoparae biased. Compared with WLPF, three wing morphs have similar morphology in bodies but is obviously discriminated in the reproductive system and fecundity. Built upon our previous study, 37 090 annotated unigenes were obtained from libraries constructed by the four morphs above through RNA-sequencing, in which 10 867, 19 334 DEGs were identified in pairwise comparison of GP vs. WLPF, Male vs. WLPF, respectively. Furthermore, 2 335 shared DEGs including 1 658 up- and 677 downregulated were obtained in these wing morphs compared to WLPF. The 1 658 shared up-regulated DEGs were enriched in multiple signaling pathways including insulin, FoxO, MAPK, strarch and sucrose metabolism, fatty acid biosynthesis and degradation which hint their key roles in the regulation of wing plasticity in cotton aphid. Gene expression levels were validated by using Pearson’s correlation (r) and potential roles of 15 DEGs related to the insulin signaling pathway in cotton wing dimorphism were discussed. Conclusions: The results of this study establish a solid foundation for deciphering molecular mechanisms underlying the switch between wingless and wing morphs in the cotton aphid and provide valuable resources for future research on the host-alternating aphid species.

Download Full-text