scholarly journals Diurnal and Seasonal Activity Patterns of Drosophilid Species (Diptera: Drosophilidae) Present in Blackberry Agroecosystems With a Focus on Spotted-Wing Drosophila

2020 ◽  
Vol 49 (2) ◽  
pp. 277-287 ◽  
Author(s):  
Katharine A Swoboda-Bhattarai ◽  
Hannah J Burrack

Abstract Drosophilid species with different life histories have been shown to exhibit similar behavioral patterns related to locating and utilizing resources such as hosts, mates, and food sources. Drosophila suzukii (Matsumura) is an invasive species that differs from other frugivorous drosophilids in that females lay eggs in ripe and ripening fruits instead of overripe or rotten fruits. We hypothesized that there may be diurnal and/or seasonal patterns associated with the movement of drosophilid species into and out of crop fields and their attraction to fermentation-odor-based monitoring traps, and that D. suzukii would conform to similar patterns. To test these hypotheses, we deployed passive, 2-headed Malaise traps between crop fields and wooded edges to simultaneously catch flies moving into and out of crop fields. We also deployed monitoring traps with a fermentation-based bait between crop fields and wooded edges and within crop rows. Traps were deployed weekly in June–August in 2014 and 2015 at two commercial blackberry farm in Cleveland County, NC, and were checked hourly for 24 h, except during darkness. Both D. suzukii and other drosophilid species moved between crop fields and wooded edges and were attracted to monitoring traps primarily during the morning and evening hours. Whereas other drosophilids were captured in traps throughout the season, few D. suzukii were caught in traps until early to mid-July in both years and increased as the season progressed. Understanding D. suzukii movement and activity patterns is essential for the development of effective management strategies.

Author(s):  
Lu-Lu Li ◽  
Ji-Wei Xu ◽  
Wei-Chen Yao ◽  
Hui-Hui Yang ◽  
Youssef Dewer ◽  
...  

Abstract The tobacco cutworm Spodoptera litura (Lepidoptera: Noctuidae) is a polyphagous pest with a highly selective and sensitive chemosensory system involved in complex physiological behaviors such as searching for food sources, feeding, courtship, and oviposition. However, effective management strategies for controlling the insect pest populations under threshold levels are lacking. Therefore, there is an urgent need to formulate eco-friendly pest control strategies based on the disruption of the insect chemosensory system. In this study, we identified 158 putative chemosensory genes based on transcriptomic and genomic data for S. litura, including 45 odorant-binding proteins (OBPs, nine were new), 23 chemosensory proteins (CSPs), 60 odorant receptors (ORs, three were new), and 30 gustatory receptors (GRs, three were new), a number higher than those reported by previous transcriptome studies. Subsequently, we constructed phylogenetic trees based on these genes in moths and analyzed the dynamic expression of various genes in head capsules across larval instars using quantitative real-time polymerase chain reaction. Nine genes–SlitOBP8, SlitOBP9, SlitOBP25, SlitCSP1, SlitCSP7, SlitCSP18, SlitOR34, SlitGR240, and SlitGR242–were highly expressed in the heads of 3- to 5-day-old S. litura larvae. The genes differentially expressed in olfactory organs during larval development might play crucial roles in the chemosensory system of S. litura larvae. Our findings substantially expand the gene inventory for S. litura and present potential target genes for further studies on larval feeding in S. litura.


Plant Disease ◽  
2004 ◽  
Vol 88 (7) ◽  
pp. 724-730 ◽  
Author(s):  
S. A. Pereyra ◽  
R. Dill-Macky ◽  
A. L. Sims

Survival and inoculum production of Gibberella zeae (Schwein.) Petch (anamorph Fusarium graminearum (Schwabe)), the causal agent of Fusarium head blight of wheat and barley, was related to the rate of wheat (Triticum aestivum L.) residue decomposition. Infested wheat residue, comprising intact nodes, internodes, and leaf sheaths, was placed in fiberglass mesh bags on the soil surface and at 7.5- to 10-cm and 15- to 20-cm depths in chisel-plowed plots and 15 to 20 cm deep in moldboard-plowed plots in October 1997. Residue was sampled monthly from April through November during 1998 and every 2 months through April to October 1999. Buried residue decomposed faster than residue placed on the soil surface. Less than 2% of the dry-matter residue remained in buried treatments after 24 months in the field, while 25% of the residue remained in the soil-surface treatment. Survival of G. zeae on node tissues was inversely related to the residue decomposition rate. Surface residue provided a substrate for G. zeae for a longer period of time than buried residue. Twenty-four months after the initiation of the trial, the level of colonization of nodes in buried residue was half the level of colonization of residue on the soil surface. Colonization of node tissues by G. zeae decreased over time, but increased for other Fusarium spp. Ascospores of G. zeae were still produced on residue pieces after 23 months, and these spores were capable of inducing disease. Data from this research may assist in developing effective management strategies for residues infested with G. zeae.


2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Hyrum S. Eddington ◽  
Courtney Carroll ◽  
Randy T. Larsen ◽  
Brock R. McMillan ◽  
John M. Chaston

Abstract Background Mule deer rely on fat and protein stored prior to the winter season as an energy source during the winter months when other food sources are sparse. Since associated microorganisms (‘microbiota’) play a significant role in nutrient metabolism of their hosts, we predicted that variation in the microbiota might be associated with nutrient storage and overwintering in mule deer populations. To test this hypothesis we performed a 16S rRNA marker gene survey of fecal samples from two deer populations in the western United States before and after onset of winter. Results PERMANOVA analysis revealed the deer microbiota varied interactively with geography and season. Further, using metadata collected at the time of sampling, we were able to identify different fecal bacterial taxa that could potentially act as bioindicators of mule deer health outcomes. First, we identified the abundance of Collinsella (family: Coriobacteriaceae) reads as a possible predictor of poor overwintering outcomes for deer herds in multiple locations. Second, we showed that reads assigned to the Bacteroides and Mollicutes Order RF39 were both positively correlated with deer protein levels, leading to the idea that these sequences might be useful in predicting mule deer protein storage. Conclusions These analyses confirm that variation in the microbiota is associated with season-dependent health outcomes in mule deer, which may have useful implications for herd management strategies.


2016 ◽  
Vol 88 (3 suppl) ◽  
pp. 1909-1921 ◽  
Author(s):  
CAMILA I. MEDEIROS ◽  
CAMILA BOTH ◽  
IGOR L. KAEFER ◽  
SONIA Z. CECHIN

ABSTRACT The North American bullfrog Lithobates catesbeianus continues to invade ecosystems worldwide, potentially causing population declines and even extinctions. Within its native distribution, bullfrogs show prolonged reproductive seasons and high fertility. However, data on breeding biology of bullfrogs ex-situ in invaded localities mainly comes from anecdotal reports. Understanding how invasive species are adjusting their life histories to new colonized environments is important for conservation purposes. Here we describe temporal and spatial abundance, calling activity, spawning and tadpole distribution of bullfrogs in southern Brazil. Eighteen samplings occurred during one year. The abundance of individuals was positively related to longer photoperiods and higher temperatures. Reproductive activity was also positively associated with longer photoperiods. Calling sites, spawning and tadpoles were associated with microhabitats presenting hydrophytes, which may provide shelter and thermal stability to bullfrogs. The reproductive seasonal activity of bullfrogs can be highly variable across its growing geographical range, but in subtropical Brazil it is associated with photoperiod, a highly predictable abiotic determinant. In our study area, bullfrogs presented a breeding season twice as long as that observed in some native localities. We suggest that management strategies directed to bullfrog populations must consider the habitat structures and seasonal regimes determined by each invaded environment.


Insects ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 994
Author(s):  
Alina S. Puig ◽  
Sarah Wurzel ◽  
Stephanie Suarez ◽  
Jean-Philippe Marelli ◽  
Jerome Niogret

Theobroma cacao is affected by viruses on every continent where the crop is cultivated, with the most well-known ones belonging to the Badnavirus genus. One of these, cacao mild mosaic virus (CaMMV), is present in the Americas, and is transmitted by several species of Pseudococcidae (mealybugs). To determine which species are associated with virus-affected cacao plants in North America, and to assess their potential as vectors, mealybugs (n = 166) were collected from infected trees in Florida, and identified using COI, ITS2, and 28S markers. The species present were Pseudococcus jackbeardsleyi (38%; n = 63), Maconellicoccus hirsutus (34.3%; n = 57), Pseudococcus comstocki (15.7%; n = 26), and Ferrisia virgata (12%; n = 20). Virus acquisition was assessed by testing mealybug DNA (0.8 ng) using a nested PCR that amplified a 500 bp fragment of the movement protein–coat protein region of CaMMV. Virus sequences were obtained from 34.6 to 43.1% of the insects tested; however, acquisition did not differ among species, X2 (3, N = 166) = 0.56, p < 0.91. This study identified two new mealybug species, P. jackbeardsleyi and M. hirsutus, as potential vectors of CaMMV. This information is essential for understanding the infection cycle of CaMMV and developing effective management strategies.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jaya Kaushik ◽  
◽  
Sandeep Shankar ◽  
Anargh V ◽  
Renu M ◽  
...  

Objective: To formulate a protocol for safe and effective management of an ophthalmologic practice in a hospital or a clinic during the Covid - 19 pandemic. Methods and analysis: Literature was reviewed regarding Covid-19 pandemic and ophthalmology. The following key words were used. Covid-19, telemedicine, challenges, management strategy. Result: An efficient protocol for ophthalmology practice during the Covid-19 pandemic was established which covered various protective measures that are essential and vital in the Covid-19 pandemic. Conclusion: An evidence-based protocol was formulated which may be modified in future as per the latest developments and trends in the ongoing pandemic.


2014 ◽  
Author(s):  
Andreas Schröder ◽  
Joel E Dimsdale

Somatic symptoms that cannot be attributed to organic disease account for 15 to 20% of primary care consultations and up to 50% in specialized settings. About 6% of the general population has chronic somatic symptoms that affect functioning and quality of life. This chapter focuses on the recognition and effective management of patients with excessive and disabling somatic symptoms. The clinical presentation of somatic symptoms is categorized into three groups of patients: those with multiple somatic symptoms, those with health anxiety, and those with conversion disorder. The chapter provides information to assist with making a diagnosis and differential diagnosis. Management includes ways to improve the physician–patient interaction that will benefit the patient, a step-care model based on illness severity and complexity, and psychological and pharmacologic treatment. The chapter is enhanced by figures and tables that summarize health anxiety, symptoms, differential diagnoses, and management strategies, as well as by case studies and examples. This review contains  5 highly rendered figures, 10 tables, and 235 references.


2018 ◽  
Author(s):  
Andreas Schröder ◽  
Joel E Dimsdale

Somatic symptoms that cannot be attributed to organic disease account for 15 to 20% of primary care consultations and up to 50% in specialized settings. About 6% of the general population has chronic somatic symptoms that affect functioning and quality of life. This chapter focuses on the recognition and effective management of patients with excessive and disabling somatic symptoms. The clinical presentation of somatic symptoms is categorized into three groups of patients: those with multiple somatic symptoms, those with health anxiety, and those with conversion disorder. The chapter provides information to assist with making a diagnosis and differential diagnosis. Management includes ways to improve the physician–patient interaction that will benefit the patient, a step-care model based on illness severity and complexity, and psychological and pharmacologic treatment. The chapter is enhanced by figures and tables that summarize health anxiety, symptoms, differential diagnoses, and management strategies, as well as by case studies and examples. This review contains  5 highly rendered figures, 10 tables, and 235 references.


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