Examination of tracheal allografts after long-term survival in dogs

Abstract OBJECTIVES Little is known on the outcome of tracheal allografts after long-term survival. This study aimed to explore the changes in structure and composition by evaluating the status of the mucosa and cartilage of allografts with long-term survival in dogs. METHODS Eight tracheal allografts that survived for ˃9 months were enrolled in our study. Epithelium, revascularization, monocyte infiltration and fibrosis were evaluated histologically. The fluorescent dye 4′-6-diamidino-2-phenylindole was used to evaluate the presence of chondrocyte nuclei. Glycosaminoglycan was detected using safranin-O staining and collagen II was evaluated using immunohistochemistry. RESULTS The 8 animals survived from 277 to 783 days. Bronchoscopy demonstrated that 6 allografts showed no stenosis; 2 cases developed slight stenosis, but could maintain airway patency. Histological examination showed that the epithelium covered the surface of the allografts. In comparison to fresh tracheal controls, allografts demonstrated mild monocyte infiltration, evident revascularization and mild fibrosis in the mucosa or submucosa (all P < 0.05). There were a few viable chondrocytes scattered in the cartilage after long-term survival. Moreover, glycosaminoglycan and collagen II were significantly decreased in the allografts compared with fresh trachea (all P < 0.05). CONCLUSIONS For tracheal allografts with long-term survival after transplantation, only a few viable chondrocytes were retained, and the extracellular matrix of the cartilage demonstrated degeneration. Despite this, the airway could maintain patency. Notably, the significance of monocyte infiltration in the mucosa or submucosa at different time points warrants further study.

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Predictive value of the Status Epilepticus Severity Score (STESS) and its components for long-term survival

BMC Neurology ◽

10.1186/s12883-016-0730-0 ◽

2016 ◽

Vol 16 (1) ◽

Cited By ~ 14

Author(s):

Preben Aukland ◽

Martin Lando ◽

Ole Vilholm ◽

Elsebeth Bruun Christiansen ◽

Christoph Patrick Beier

Keyword(s):

Status Epilepticus ◽

Predictive Value ◽

Severity Score ◽

Term Survival ◽

Long Term Survival ◽

The Status

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The Seldom Acknowledged Difficulties of Leading Missional Churches: Challenges Faced by Those Who Seek to Explore Different Forms of Being and Doing Church

Ecclesial Practices ◽

10.1163/22144471-00601006 ◽

2019 ◽

Vol 6 (2) ◽

pp. 163-181

Author(s):

Andrew A. Groza

Keyword(s):

Term Survival ◽

Long Term Survival ◽

Australian Context ◽

Missional Theology ◽

The Status ◽

Missional Communities ◽

Missional Churches ◽

The Church

The Church in Australia finds itself pushed to the margins of society and lacking the status it once enjoyed in previous generations. The importance and role of the church in society’s life is now questioned and it would seem that the church’s long term survival is being challenged. How should the church respond? One response is found in the exploration of new forms of church birthed out of missional theology – a theology that sees the church partnering with the God who is actively on mission in his world. This response however, does not come without its own inherent challenges. Leaders of missional communities within the Australian context were interviewed in an attempt to decipher what those challenges might be. The results can largely be covered under the rubrics of: Ambiguity, Anxiety, Audience and Abandonment.

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Renal Replacement Therapy in Patients With Stage IV Cancer Admitted to the Intensive Care Unit With Acute Kidney Injury at a Comprehensive Cancer Center Was Not Associated With Survival

American Journal of Hospice and Palliative Medicine® ◽

10.1177/1049909120902115 ◽

2020 ◽

Vol 37 (9) ◽

pp. 707-715

Author(s):

Ala Abudayyeh ◽

Juhee Song ◽

Maen Abdelrahim ◽

Ibrahim Dahbour ◽

Valda D. Page ◽

...

Keyword(s):

Intensive Care Unit ◽

Kidney Injury ◽

Stage Iv ◽

Inpatient Mortality ◽

Survival Times ◽

Term Survival ◽

Long Term Survival ◽

Time Points ◽

Stage Iv Cancer

Introduction: In patients with advanced cancer, prolongation of life with treatment often incurs substantial emotional and financial expense. Among hospitalized patients with cancer since acute kidney injury (AKI) is known to be associated with much higher odds for hospital mortality, we investigated whether renal replacement therapy (RRT) use in the intensive care unit (ICU) was a significant independent predictor of worse outcomes. Methods: We retrospectively reviewed patients admitted in 2005 to 2014 who were diagnosed with stage IV solid tumors, had AKI, and a nephrology consult. The main outcomes were survival times from the landmark time points, inpatient mortality, and longer term survival after hospital discharge. Logistic regression and Cox proportional regression were used to compare inpatient mortality and longer term survival between RRT and non-RRT groups. Propensity score-matched landmark survival analyses were performed with 2 landmark time points chosen at day 2 and at day 7 from ICU admission. Results: Of the 465 patients with stage IV cancer admitted to the ICU with AKI, 176 needed RRT. In the multivariate logistic regression model after adjusting for baseline serum albumin and baseline maximum Sequential Organ Failure Assessment (SOFA), the patients who received RRT were not significantly different from non-RRT patients in inpatient mortality (odds ratio: 1.004 [95% confidence interval: 0.598-1.684], P = .9892). In total, 189 patients were evaluated for the impact of RRT on long-term survival and concluded that RRT was not significantly associated with long-term survival after discharge for patients who discharged alive. Landmark analyses at day 2 and day 7 confirmed the same findings. Conclusions: Our study found that receiving RRT in the ICU was not significantly associated with inpatient mortality, survival times from the landmark time points, and long-term survival after discharge for patients with stage IV cancer with AKI.

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Defining the Extracellular Matrix of Rhabdomyosarcoma

10.21203/rs.3.rs-38028/v1 ◽

2020 ◽

Author(s):

Xiaolei Lian ◽

J. Steffan Bond ◽

Narendra Bharathy ◽

Sergei P. Boudko ◽

Elena Pokidysheva ◽

...

Keyword(s):

Extracellular Matrix ◽

Soft Tissue ◽

Structural Integrity ◽

Quaternary Structure ◽

Soft Tissue Sarcomas ◽

Tissue Microarrays ◽

Cell Of Origin ◽

Term Survival ◽

Long Term Survival

Abstract Background: Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma of childhood with a propensity to metastasize. Current treatment for patients with RMS includes conventional systemic chemotherapy, radiation therapy and surgical resection; nevertheless, little to no improvement in long term survival has been achieved in decades – underlining the need for target discovery and new therapeutic approaches to targeting tumor cells or the tumor microenvironment.Methods:To evaluate cross-species sarcoma extracellular matrix production, we have used murine models which feature knowledge of the myogenic cell-of-origin. With focus on the RMS/undifferentiated pleomorphic sarcoma (UPS) continuum, we have constructed tissue microarrays of 48 murine and 4 human sarcomas to analyze expression of 7 different collagens, fibrillins and collagen-modifying proteins, with cross-correlation to RNA deep sequencing.Results:We have uncovered that RMS produces increased expression of type XVIII collagen alpha 1 (COL18A1), which is clinically associated with decreased long-term survival. We have also identified significantly increased RNA expression of COL4A1, FBN2, PLOD1 and PLOD2 in human RMS relative to normal skeletal muscle.Conclusion:These results complement recent studies investigating whether soft tissue sarcomas utilize collagens, fibrillins and collagen-modifying enzymes to alter the structural integrity of surrounding host extracellular matrix/collagen quaternary structure resulting in improved ability to improve the ability to invade regionally and metastasize, for which therapeutic targeting is possible.

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Defining the Extracellular Matrix of Rhabdomyosarcoma

Frontiers in Oncology ◽

10.3389/fonc.2021.601957 ◽

2021 ◽

Vol 11 ◽

Author(s):

Xiaolei Lian ◽

J. Steffan Bond ◽

Narendra Bharathy ◽

Sergei P. Boudko ◽

Elena Pokidysheva ◽

...

Keyword(s):

Extracellular Matrix ◽

Soft Tissue ◽

Structural Integrity ◽

Quaternary Structure ◽

Soft Tissue Sarcomas ◽

Tissue Microarrays ◽

Cell Of Origin ◽

Term Survival ◽

Long Term Survival

Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma of childhood with a propensity to metastasize. Current treatment for patients with RMS includes conventional systemic chemotherapy, radiation therapy, and surgical resection; nevertheless, little to no improvement in long term survival has been achieved in decades—underlining the need for target discovery and new therapeutic approaches to targeting tumor cells or the tumor microenvironment. To evaluate cross-species sarcoma extracellular matrix production, we have used murine models which feature knowledge of the myogenic cell-of-origin. With focus on the RMS/undifferentiated pleomorphic sarcoma (UPS) continuum, we have constructed tissue microarrays of 48 murine and four human sarcomas to analyze expression of seven different collagens, fibrillins, and collagen-modifying proteins, with cross-correlation to RNA deep sequencing. We have uncovered that RMS produces increased expression of type XVIII collagen alpha 1 (COL18A1), which is clinically associated with decreased long-term survival. We have also identified significantly increased RNA expression of COL4A1, FBN2, PLOD1, and PLOD2 in human RMS relative to normal skeletal muscle. These results complement recent studies investigating whether soft tissue sarcomas utilize collagens, fibrillins, and collagen-modifying enzymes to alter the structural integrity of surrounding host extracellular matrix/collagen quaternary structure resulting in improved ability to improve the ability to invade regionally and metastasize, for which therapeutic targeting is possible.

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Long-term survival of femoral neck fracture patients age over ninety: Arthroplasty compare with conservative treatment

10.21203/rs.2.21177/v1 ◽

2020 ◽

Author(s):

Yang Liu ◽

Chong-wei Zhang ◽

Xiao-dan Zhao

Keyword(s):

Treatment Group ◽

Conservative Treatment ◽

Femoral Neck ◽

Femoral Neck Fracture ◽

Term Survival ◽

Long Term Survival ◽

Time Points ◽

Neck Fracture ◽

The Difference

Abstract Background The aging of the China population is expected to lead to increasing of nonagenarian and centenarian.The mortality rates of nonagenarian hip fracture patients would return to an equivalent mortality risk to the normal population at five years after injury. It is imperative to evaluate the 5-year mortality for this small but very challenging subgroup patients in order to optimize patient management. The main purpose of the current retrospective study was to compare the five-year survival between arthroplasty treatment and conservative treatment of femoral neck fracture patients age over 90 years during the same 16 -year period.Methods From January 1998 to December 2014, all consecutive nonagenarian and centenarian patients with femoral neck fracture admitted to our hospital were included for evaluation. The primary outcome was defined as thirty-day mortality, 1-year, 3-year, and 5-year mortality after injury. Survival status analysis was performed by the Kaplan–Meier method for mortality. Using the log-rank test, the stratified analyses were performed to compare the difference of overall cumulative mortality and three-time points (1-year, 3-year, and 5-year) mortality after injury were performed to compare the difference of survival distributions.Results Over the 16-year study period, the arthroplasty group and the conservative treatment group included 33 and 53 patients, respectively. The long-term survival probability of the arthroplasty group is significantly higher than the conservative treatment group( p=0.002277). The survival time of the arthroplasty group is significantly higher than the conservative treatment group(Median(P75-P25)=53(59) versus Median(P75-P25)=22(52), p=0.001). The difference of five time points (1-year, 2-year, 3-year, 4-year, and 5-year) mortality between the conservative group and arthroplasty group is significant except for 30-day mortality.The stratified analyses of overall cumulative mortality and three-time points (1-year, 3-year, and 5-year) mortality after injury demonstrated that the arthroplasty group is significant higher than the conservative treatment group.Conclusions Our study demonstrate that, compared with conservative treatment, arthroplasty surgery is more likely to improve the long-term survival of femoral neck fracture patients over 90s. What can be expected is that nearly half of patients will survive more than five years after surgery.

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Esophageal regeneration following surgical implantation of a tissue engineered esophageal implant in a pediatric model

npj Regenerative Medicine ◽

10.1038/s41536-021-00200-9 ◽

2022 ◽

Vol 7 (1) ◽

Author(s):

Sumati Sundaram ◽

Todd Jensen ◽

Tina Roffidal ◽

Karissa Paquin ◽

Heather Wanczyk ◽

...

Keyword(s):

Survival Time ◽

Tissue Regeneration ◽

Time Course ◽

Ct Imaging ◽

Congenital Defects ◽

Term Survival ◽

Long Term Survival ◽

Time Points ◽

Post Implantation

AbstractDiseases of the esophagus, damage of the esophagus due to injury or congenital defects during fetal esophageal development, i.e., esophageal atresia (EA), typically require surgical intervention to restore esophageal continuity. The development of tissue engineered tubular structures would improve the treatment options for these conditions by providing an alternative that is organ sparing and can be manufactured to fit the exact dimensions of the defect. An autologous tissue engineered Cellspan Esophageal ImplantTM (CEI) was surgically implanted into piglets that underwent surgical resection of the esophagus. Multiple survival time points, post-implantation, were analyzed histologically to understand the tissue architecture and time course of the regeneration process. In addition, we investigated CT imaging as an “in-life” monitoring protocol to assess tissue regeneration. We also utilized a clinically relevant animal management paradigm that was essential for long term survival. Following implantation, CT imaging revealed early tissue deposition and the formation of a contiguous tissue conduit. Endoscopic evaluation at multiple time points revealed complete epithelialization of the lumenal surface by day 90. Histologic evaluation at several necropsy time points, post-implantation, determined the time course of tissue regeneration and demonstrated that the tissue continues to remodel over the course of a 1-year survival time period, resulting in the development of esophageal structural features, including the mucosal epithelium, muscularis mucosae, lamina propria, as well as smooth muscle proliferation/migration initiating the formation of a laminated adventitia. Long term survival (1 year) demonstrated restoration of oral nutrition, normal animal growth and the overall safety of this treatment regimen.

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