Antifungal activity of selected natural preservatives against the foodborne molds Penicillium verrucosum and Aspergillus westerdijkiae

2018 ◽  
Vol 365 (13) ◽  
Author(s):  
Inga Schlösser ◽  
Alexander Prange
Keyword(s):  
Antifungal Activity ◽  
Penicillium Verrucosum ◽  
Aspergillus Westerdijkiae ◽  
Natural Preservatives

Antifungal Activity of Selected Natural Preservatives against Aspergillus westerdijkiae and Penicillium verrucosum and the Interactions of These Preservatives with Food Components

2019 ◽  
Vol 82 (10) ◽  
pp. 1751-1760
Author(s):  
INGA SCHLÖSSER ◽  
ALEXANDER PRANGE
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Inhibitory Effect ◽  
Malt Extract ◽  
Penicillium Verrucosum ◽  
Origanum Vulgare ◽  
Food Components ◽  
Aspergillus Westerdijkiae ◽  
Natural Preservatives ◽  
Food Model

ABSTRACT The present study examined the influence of primary food components on the antifungal activity of the essential oil of Origanum vulgare, carvacrol, thymol, eugenol, and trans-cinnamaldehyde against Penicillium verrucosum and Aspergillus westerdijkiae. The MIC was determined in food model media enriched with proteins (1, 5, or 10%), carbohydrates (1, 4, or 6%), or oil (1, 5, or 10%). Proteins increased the antifungal activity of O. vulgare essential oil, carvacrol, thymol, and eugenol, whereas the effect of trans-cinnamaldehyde decreased with increasing protein content. The presence of carbohydrates diminished the inhibitory effect of the natural preservatives on A. westerdijkiae; for P. verrucosum, their inhibitory effect increased with carbohydrates. Only the antifungal activity of trans-cinnamaldehyde did not depend on the carbohydrate content. The presence of oil had the strongest influence. At a concentration of 1% oil, the antifungal activity decreased significantly, and at 10% oil, almost no inhibition was observed. To investigate the effect of the antifungal agents on the morphology of the target molds, they were grown on malt extract agar containing carvacrol and trans-cinnamaldehyde and were examined by scanning electron microscopy. The hyphae, conidiophores, vesicles, and phialides were severely altered and deformed, and spore formation was clearly suppressed.

scholarly journals In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius

Toxins ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 700 ◽  
Author(s):  
Randa Zeidan ◽  
Zahoor Ul-Hassan ◽  
Roda Al-Thani ◽  
Quirico Migheli ◽  
Samir Jaoua
Keyword(s):  
Antifungal Activity ◽  
Ochratoxin A ◽  
Burkholderia Cepacia ◽  
Culture Supernatant ◽  
Animal Health ◽  
Aspergillus Carbonarius ◽  
Penicillium Verrucosum ◽  
Wide Range ◽  
Mycotoxigenic Fungi

Mycotoxins are secondary metabolites produced by certain filamentous fungi, causing human and animal health issues upon the ingestion of contaminated food and feed. Among the safest approaches to the control of mycotoxigenic fungi and mycotoxin detoxification is the application of microbial biocontrol agents. Burkholderia cepacia is known for producing metabolites active against a broad number of pathogenic fungi. In this study, the antifungal potential of a Qatari strain of Burkholderia cepacia (QBC03) was explored. QBC03 exhibited antifungal activity against a wide range of mycotoxigenic, as well as phytopathogenic, fungal genera and species. The QBC03 culture supernatant significantly inhibited the growth of Aspergillus carbonarius, Fusarium culmorum and Penicillium verrucosum in PDA medium, as well as A. carbonarius and P. verrucosum biomass in PDB medium. The QBC03 culture supernatant was found to dramatically reduce the synthesis of ochratoxin A (OTA) by A. carbonarius, in addition to inducing mycelia malformation. The antifungal activity of QBC03’s culture extract was retained following thermal treatment at 100 °C for 30 min. The findings of the present study advocate that QBC03 is a suitable biocontrol agent against toxigenic fungi, due to the inhibitory activity of its thermostable metabolites.

In vitro antifungal activity of residues from essential oil industry against Penicillium verrucosum, a common contaminant of ripening cheeses

LWT ◽  
2016 ◽  
Vol 73 ◽  
pp. 226-232 ◽  
Author(s):  
Gonzalo Ortiz de Elguea-Culebras ◽  
Raúl Sánchez-Vioque ◽  
Omar Santana-Méridas ◽  
David Herraiz-Peñalver ◽  
Manuel Carmona ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Oil Industry ◽  
Penicillium Verrucosum ◽  
In Vitro Antifungal Activity

scholarly journals Biosynthesis of silver nanoparticles using Penicillium verrucosum and analysis of their antifungal activity

2021 ◽  
Author(s):  
Mohamed A. Yassin ◽  
Abdallah M. Elgorban ◽  
Abd El-Rahim M.A. El-Samawaty ◽  
Bandar M.A. Almunqedhi
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Penicillium Verrucosum

scholarly journals Development of an Antifungal and Antimycotoxigenic Device Containing Allyl Isothiocyanate for Silo Fumigation

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 137 ◽  
Author(s):  
Juan Quiles ◽  
Tiago Nazareth ◽  
Carlos Luz ◽  
Fernando Luciano ◽  
Jordi Mañes ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ochratoxin A ◽  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Fungal Growth ◽  
Allyl Isothiocyanate ◽  
Bioactive Compound ◽  
Previous Trial ◽  
Control Test ◽  
Penicillium Verrucosum

The aims of this study were to evaluate the antifungal activity of the bioactive compound allyl isothiocyanate (AITC) against Aspergillus flavus (8111 ISPA) aflatoxins (AFs) producer and Penicillium verrucosum (D-01847 VTT) ochratoxin A (OTA) producer on corn, barley, and wheat. The experiments were carried out initially in a simulated silo system for laboratory scale composed of glass jars (1 L). Barley and wheat were contaminated with P. verrucosum and corn with A. flavus. The cereals were treated with a hydroxyethylcellulose gel disk to which 500 µL/L of AITC were added; the silo system was closed and incubated for 30 days at 21 °C. After that, simulated silos of 100 L capacity were used. Barley, wheat, and corn were contaminated under the same conditions as the previous trial and treated with disks with 5 mL of AITC, closed and incubated for 90 days at 21 °C. In both cases, the control test did not receive any antifungal treatment. The growth of the inoculated fungi and the reduction in the formation of AFs and OTA were determined. In the lab scale silo system, complete inhibition of fungal growth at 30 days has been observed. In corn, the reduction of aflatoxin B1 (AFB1) was 98.5%. In the 100 L plastic drums, a significant reduction in the growth of A. flavus was observed, as well as the OTA formation in wheat (99.5%) and barley (92.0%).

scholarly journals Evaluation of Essential Oils and Extracts of Rose Geranium and Rose Petals as Natural Preservatives in Terms of Toxicity, Antimicrobial, and Antiviral Activity

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 494
Author(s):  
Chrysa Androutsopoulou ◽  
Spyridoula D. Christopoulou ◽  
Panagiotis Hahalis ◽  
Chrysoula Kotsalou ◽  
Fotini N. Lamari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antiviral Activity ◽  
Essential Oils ◽  
Life Span ◽  
Rosa Damascena ◽  
E Coli ◽  
Serovar Typhimurium ◽  
Rose Petals ◽  
Natural Preservatives

Essential oils (EOs) and extracts of rose geranium (Pelargonium graveolens) and petals of rose (Rosa damascena) have been fully characterized in terms of composition, safety, antimicrobial, and antiviral properties. They were analyzed against Escherichia coli, Salmonella enterica serovar Typhimurium, Staphylococcus aureus, Aspergillus niger, and Adenovirus 35. Their toxicity and life span were also determined. EO of P. graveolens (5%) did not retain any antibacterial activity (whereas at 100% it was greatly effective against E. coli), had antifungal activity against A. niger, and significant antiviral activity. Rose geranium extract (dilutions 25−90%) (v/v) had antifungal and antibacterial activity, especially against E. coli, and dose-dependent antiviral activity. Rose petals EO (5%) retains low inhibitory activity against S. aureus and S. Typhimurium growth (about 20−30%), antifungal activity, and antiviral activity for medium to low virus concentrations. Rose petals extract had significant antibacterial activity at dilutions of 25−90%, especially against E. coli and S. Typhimurium, antifungal, and the most potent antiviral activity. None of the EOs and extracts were toxic in dilutions of up to 5% and 90%, respectively. Finally, all materials had a life span of more than eight weeks. These results support the aspect that rose petals and rose geranium EOs, and extracts, have beneficial antimicrobial and antiviral properties and they can be used as natural preservatives.

scholarly journals Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by Aspergillus westerdijkiae and Penicillium verrucosum

2020 ◽  
Vol 21 (22) ◽  
pp. 8548
Author(s):  
Saranyaphat Boonmee ◽  
Vessela Atanasova ◽  
Sylvain Chéreau ◽  
Gisèle Marchegay ◽  
Kevin D. Hyde ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fungal Growth ◽  
Culture Conditions ◽  
Growth Reduction ◽  
Penicillium Verrucosum ◽  
Aspergillus Westerdijkiae ◽  
Synthetic Pesticides ◽  
Definition Of ◽  
The Impact ◽  
Potential Use

Ochratoxin A (OTA) is one of the worldwide most important mycotoxins in terms of health and agroeconomic consequences. With the aim to promote the use of phytochemicals as alternatives to synthetic fungicides, the effect of hydroxycinnamic acids on the fungal growth and OTA yield by two major OTA-producing species was investigated. After a first step dedicated to the definition of most suitable culture conditions, the impact of 0.5 mM ferulic (FER), p-coumaric (COUM), caffeic and chlorogenic acids was evaluated on Aspergillus westerdijkiae and Penicillium verrucosum. Whereas no fungal growth reduction was observed regardless of the phenolic acid and fungal isolate, our results demonstrated the capacity of FER and COUM to inhibit OTA production. The most efficient compound was FER that led to a 70% reduction of OTA yielded by P. verrucosum and, although not statistically significant, a 35% inhibition of OTA produced by A. westerdijkiae. To further investigate the bioactivity of FER and COUM, their metabolic fate was characterized in fungal broths. The capacity of P. verrucosum to metabolize FER and COUM through a C2-clivage type degradation was demonstrated. Overall, our data support the potential use of FER to prevent OTA contamination and reduce the use of synthetic pesticides.

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