The rpf/DSF signalling system of Stenotrophomonas maltophilia positively regulates biofilm formation, production of virulence-associated factors and β-lactamase induction

2019 ◽  
Vol 366 (6) ◽  
Author(s):  
Eliana Alcaraz ◽  
Carlos García ◽  
Laura Friedman ◽  
Beatriz Passerini de Rossi
Keyword(s):  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Associated Factors ◽  
Signalling System

scholarly journals Biofilm Formation by Stenotrophomonas maltophilia: Modulation by Quinolones, Trimethoprim-Sulfamethoxazole, and Ceftazidime

2004 ◽  
Vol 48 (1) ◽  
pp. 151-160 ◽  
Author(s):  
Giovanni Di Bonaventura ◽  
Ilaria Spedicato ◽  
Domenico D'Antonio ◽  
Iole Robuffo ◽  
Raffaele Piccolomini
Keyword(s):  
Cell Viability ◽  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Maximum Growth ◽  
Total Biomass ◽  
Significant Activity ◽  
Original Mass ◽  
In Vitro Effects ◽  
Over Time

ABSTRACT We investigated the in vitro effects of seven fluoroquinolones (ciprofloxacin, grepafloxacin, levofloxacin, moxifloxacin, norfloxacin, ofloxacin, and rufloxacin), compared to those of trimethoprim-sulfamethoxazole (SXT) and ceftazidime on total biomass and cell viability of Stenotrophomonas maltophilia biofilm. S. maltophilia attached rapidly to polystyrene, within 2 h of incubation, and then biofilm formation increased over time, reaching maximum growth at 24 h. In the presence of fluoroquinolones at one-half and one-fourth the MIC, biofilm biomass was significantly (P < 0.01) reduced to 55 to 70% and 66 to 76% of original mass, respectively. Ceftazidime and SXT did not exert any activity. Biofilm bacterial viability was significantly reduced by all antibiotics tested at one-half the MIC. At one-fourth the MIC all antibiotics, except levofloxacin, significantly reduced viability. Treatment of preformed biofilms with bactericidal concentrations (500, 100, and 50 μg/ml) of all fluoroquinolones caused, except for norfloxacin, significant reduction of biofilm biomass to 29.5 to 78.8, 64.1 to 83.6, and 70.5 to 82.8% of original mass, respectively. SXT exerted significant activity at 500 μg/ml only. Ceftazidime was completely inactive. Rufloxacin exhibited the highest activity on preformed biofilm viability, significantly decreasing viable counts by 0.6, 5.4, and 17.1% at 500, 100, and 50 μg/ml, respectively. Our results show that (i) subinhibitory (one-half and one-fourth the MIC) concentrations of fluoroquinolones inhibit adherence of S. maltophilia to polystyrene and (ii) clinically achievable concentrations (50 and 100 μg/ml) of rufloxacin are able to eradicate preformed S. maltophilia biofilm.

scholarly journals Effects of Simulated Microgravity on the Physiology of Stenotrophomonas maltophilia and Multiomic Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaolei Su ◽  
Yinghua Guo ◽  
Tingzheng Fang ◽  
Xuege Jiang ◽  
Dapeng Wang ◽  
...  
Keyword(s):  
Growth Rate ◽  
Differentially Expressed Genes ◽  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Simulated Microgravity ◽  
Normal Gravity ◽  
Differentially Expressed ◽  
Space Environment ◽  
Biological Adhesion ◽  
Proteomic Analyses

Many studies have shown that the space environment plays a pivotal role in changing the characteristics of conditional pathogens, especially their pathogenicity and virulence. However, Stenotrophomonas maltophilia, a type of conditional pathogen that has shown to a gradual increase in clinical morbidity in recent years, has rarely been reported for its impact in space. In this study, S. maltophilia was exposed to a simulated microgravity (SMG) environment in high-aspect ratio rotating-wall vessel bioreactors for 14days, while the control group was exposed to the same bioreactors in a normal gravity (NG) environment. Then, combined phenotypic, genomic, transcriptomic, and proteomic analyses were conducted to compare the influence of the SMG and NG on S. maltophilia. The results showed that S. maltophilia in simulated microgravity displayed an increased growth rate, enhanced biofilm formation ability, increased swimming motility, and metabolic alterations compared with those of S. maltophilia in normal gravity and the original strain of S. maltophilia. Clusters of Orthologous Groups (COG) annotation analysis indicated that the increased growth rate might be related to the upregulation of differentially expressed genes (DEGs) involved in energy metabolism and conversion, secondary metabolite biosynthesis, transport and catabolism, intracellular trafficking, secretion, and vesicular transport. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the increased motility might be associated the upregulation of differentially expressed proteins (DEPs) involved in locomotion, localization, biological adhesion, and binding, in accordance with the upregulated DEGs in cell motility according to COG classification, including pilP, pilM, flgE, flgG, and ronN. Additionally, the increased biofilm formation ability might be associated with the upregulation of DEPs involved in biofilm formation, the bacterial secretion system, biological adhesion, and cell adhesion, which were shown to be regulated by the differentially expressed genes (chpB, chpC, rpoN, pilA, pilG, pilH, and pilJ) through the integration of transcriptomic and proteomic analyses. These results suggested that simulated microgravity might increase the level of corresponding functional proteins by upregulating related genes to alter physiological characteristics and modulate growth rate, motility, biofilm formation, and metabolism. In conclusion, this study is the first general analysis of the phenotypic, genomic, transcriptomic, and proteomic changes in S. maltophilia under simulated microgravity and provides some suggestions for future studies of space microbiology.

Global analyses imply that Stenotrophomonas maltophilia biofilms are phenotypically highly diverse despite a common transcriptome profile

2020 ◽  
Author(s):  
Ifey Alio ◽  
Mirja Gudzuhn ◽  
Marie Schölmerich ◽  
Pablo Pérez García ◽  
Christel Vollstedt ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Phenotypic Variability ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Clinical Settings ◽  
Data Set ◽  
Specific Level ◽  
Key Factor ◽  
Fermentative Growth

&lt;p&gt;&lt;strong&gt;Stenotrophomonas maltophilia&lt;/strong&gt;&lt;strong&gt; is one of the most frequently isolated multidrug resistant opportunistic pathogens. It contributes to disease progression in cystic fibrosis patients and is found in wounds, other infected tissues and on catheter surfaces. Only little is known on key processes linked to biofilm formation of S. maltophilia on a broader basis. Thus the aim of this study was the identification of key processes involved in biofilm formation of S. maltophilia on a global level. Therefore, we analyzed biofilm profiles of 300 globally collected clinical and environmental isolates of the main and recently identified lineages Sgn3, Sgn4 and Sm2 - Sm18 (Groeschel et al., 2020). These data together with the 3D structural analysis of a subset of clinical 40 clinical isolates revealed an unexpectedly high phenotypic variability on a strain specific level. Further transcriptome analysis of seven clinical isolates using biofilm grown cells identified a set of 106 shared and coexpressed genes and roughly 30-35 strain-specific genes. Based on these findings S. maltophilia employs a mostly fermentative growth modus under the biofilm conditions and uptake of iron, phosphorous and other metals appears to be of high relevance. Surprisingly, the transcriptome profiles of biofilm versus planktonic cells revealed that only 8.6% of all genes were differentially regulated when both conditions were compared.&amp;#160; This implies that only relatively few genes contribute to the change from planktonic to biofilm life style. Thereby iron uptake appears to be a key factor involved in this metabolic shift. The transcriptome data generated in this study together with the phenotypic and metabolic analysis represent so far the largest data set on S. maltophilia biofilm versus planktonic grown cells. This study now lays the foundation for the identification of new strategies in fighting S. maltophilia infections in clinical settings.&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Ref: &amp;#160;Gr&amp;#246;schel et al., 2020 ,The phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia. Nature Commun. 2020 Apr 27;11(1):2044. doi: 10.1038/s41467-020-15123-0.&lt;/p&gt;

scholarly journals BsmR degrades c-di-GMP to modulate biofilm formation of nosocomial pathogen Stenotrophomonas maltophilia

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Wei Liu ◽  
Xiu-Qi Tian ◽  
Jin-Wei Wei ◽  
Li-Li Ding ◽  
Wei Qian ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Nosocomial Pathogen

scholarly journals The Ax21 protein influences virulence and biofilm formation in the nosocomial pathogen Stenotrophomonas maltophilia

2017 ◽  
Author(s):  
Shi-qi An ◽  
Ji-liang Tang
Keyword(s):  
Antibiotic Resistance ◽  
Plant Pathogen ◽  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Xanthomonas Oryzae ◽  
Nosocomial Pathogen ◽  
Antibiotic Resistant ◽  
Gram Negative ◽  
Hospital Acquired Infection ◽  
Hospital Acquired

Stenotrophomonas maltophilia is an antibiotic resistant Gram-negative pathogen, which is associated with hospital-acquired infection. The genome encodes a protein highly related to the Ax21 protein of Xanthomonas oryzae pv. oryzae that is implicated in interactions of this plant pathogen with rice. Here we show that loss by mutation of Ax21 influences a variety of functions in Stenotrophomonas maltophilia, to include virulence, antibiotic resistance and biofilm formation in this nosocomial pathogen.

scholarly journals Author Correction: BsmR degrades c-di-GMP to modulate biofilm formation of nosocomial pathogen Stenotrophomonas maltophilia

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Wei Liu ◽  
Xiu-Qi Tian ◽  
Jin-Wei Wei ◽  
Li-Li Ding ◽  
Wei Qian ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Nosocomial Pathogen

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.

High occurrence of antibiotic resistance and biofilm-formation among Stenotrophomonas maltophilia isolated from a tertiary hospital in Southwest of Iran

Gene Reports ◽  
2020 ◽  
Vol 21 ◽  
pp. 100827 ◽  
Author(s):  
Neda Mohagheghzadeh ◽  
Zahra Hashemizadeh ◽  
Reza Khashei ◽  
Soudeh Kholdi ◽  
Samane Mohebi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Tertiary Hospital ◽  
High Occurrence ◽  
Southwest Of Iran

scholarly journals Diffusible Signal Factor signaling regulates multiple functions in the opportunistic pathogen Stenotrophomonas maltophilia

2018 ◽  
Author(s):  
Shi-qi An ◽  
Ji-liang Tang
Keyword(s):  
Nosocomial Infections ◽  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Opportunistic Pathogen ◽  
Negative Bacterium ◽  
Signaling Mechanism ◽  
Gram Negative ◽  
Multiple Functions ◽  
Diffusible Signal Factor ◽  
Dependent Cell

AbstractStenotrophomonas maltophilia is a Gram-negative bacterium commonly isolated from nosocomial infections. Analysis of the genome of the clinical Stenotrophomonas maltophilia isolate K279a indicates that it encodes a diffusible signal factor (DSF)-dependent cell-cell signaling mechanism that is highly similar to the system previously described in phytopathogens from the genera Xanthomonas and Xylella. Here we demonstrate that in S. maltophilia strain K279a, DSF signaling regulates factors contributing to virulence, biofilm formation and motility of this important opportunistic pathogen.

scholarly journals Putative Iron Acquisition Systems in Stenotrophomonas maltophilia

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2048 ◽  
Author(s):  
V. Kalidasan ◽  
Adleen Azman ◽  
Narcisse Joseph ◽  
Suresh Kumar ◽  
Rukman Awang Hamat ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Stenotrophomonas Maltophilia ◽  
Chemical Nature ◽  
Colorimetric Assay ◽  
Iron Acquisition ◽  
Environmental Isolates ◽  
Iron Starvation ◽  
Receptor System ◽  
Cas Assay ◽  
Significant Expression

Iron has been shown to regulate biofilm formation, oxidative stress response and several pathogenic mechanisms in Stenotrophomonas maltophilia. Thus, the present study is aimed at identifying various iron acquisition systems and iron sources utilized during iron starvation in S. maltophilia. The annotations of the complete genome of strains K279a, R551-3, D457 and JV3 through Rapid Annotations using Subsystems Technology (RAST) revealed two putative subsystems to be involved in iron acquisition: the iron siderophore sensor and receptor system and the heme, hemin uptake and utilization systems/hemin transport system. Screening for these acquisition systems in S. maltophilia showed the presence of all tested functional genes in clinical isolates, but only a few in environmental isolates. NanoString nCounter Elements technology, applied to determine the expression pattern of the genes under iron-depleted condition, showed significant expression for FeSR (6.15-fold), HmuT (12.21-fold), Hup (5.46-fold), ETFb (2.28-fold), TonB (2.03-fold) and Fur (3.30-fold). The isolates, when further screened for the production and chemical nature of siderophores using CAS agar diffusion (CASAD) and Arnows’s colorimetric assay, revealed S. maltophilia to produce catechol-type siderophore. Siderophore production was also tested through liquid CAS assay and was found to be greater in the clinical isolate (30.8%) compared to environmental isolates (4%). Both clinical and environmental isolates utilized hemoglobin, hemin, transferrin and lactoferrin as iron sources. All data put together indicates that S. maltophilia utilizes siderophore-mediated and heme-mediated systems for iron acquisition during iron starvation. These data need to be further confirmed through several knockout studies.

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