scholarly journals Diversity of cyanobacteria from thermal muds (Balaruc-Les-Bains, France) with the description of Pseudochroococcus coutei gen. nov., sp. nov.

FEMS Microbes ◽  
2021 ◽  
Vol 2 ◽  
Author(s):  
C Duval ◽  
S Hamlaoui ◽  
B Piquet ◽  
G Toutirais ◽  
C Yéprémian ◽  
...  
Keyword(s):  
New Genus ◽  
Its Region ◽  
Taxonomic Diversity ◽  
Ecological Niches ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Genetic Characteristics ◽  
Retention Basin ◽  
Therapeutic Properties ◽  
High Diversity

ABSTRACT Cyanobacteria are able to synthesize a high diversity of natural compounds that account for their success in the colonization of a variety of ecological niches. Many of them have beneficial properties. The mud from the thermal baths of Balaruc-Les-Bains, one of the oldest thermal baths in France, has long been recognized as a healing treatment for arthro-rheumatic diseases. To characterize the cyanobacteria living in these muds, several strains were isolated from the water column and biofilms of the retention basin and analyzed using a polyphasic approach. Morphological, ultrastructural and molecular (16S rRNA gene and 16S-23S ITS region sequencing) methods were employed to identify nine cyanobacterial strains belonging to the orders Chroococcales, Synechococcales, Oscillatoriales and Nostocales. The combination of morphological and genetic characteristics supported the description of a new genus and species with the type species as Pseudochroococcus coutei. The taxonomic diversity in the muds from Thermes de Balaruc-Les-Bains appears higher than previously documented, providing new candidate taxa for their observed therapeutic properties.

scholarly journals Characterization of cyanobacteria isolated from thermal muds of Balaruc- Les-Bains (France) and description of a new genus and species Pseudochroococcus couteii

2020 ◽  
Author(s):  
Charlotte Duval ◽  
Sahima Hamlaoui ◽  
Berenice Piquet ◽  
Geraldine Toutirais ◽  
Claude Yepremian ◽  
...  
Keyword(s):  
New Genus ◽  
Its Region ◽  
Taxonomic Diversity ◽  
Ecological Niches ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Genetic Characteristics ◽  
Retention Basin ◽  
Metabolite Synthesis

Cyanobacteria are able to synthesize a high diversity of natural compounds that account for their success in the colonization of a variety of ecological niches. Many of them have beneficial properties. The mud from the thermal baths of Balaruc-Les-Bains, one of the oldest thermal baths in France, has long been recognized as a healing treatment for arthro-rheumatic diseases. To characterize the cyanobacteria living in these muds and the metabolites they potentially produce, several strains were isolated from the water column and biofilms of the retention basin and analyzed using a polyphasic approach. Morphological, ultrastructural and molecular (16S rRNA gene and 16S-23S ITS region sequencing) methods were employed to identify nine cyanobacterial strains belonging to the orders Chroococcales, Synechococcales, Oscillatoriales and Nostocales. The combination of morphological and genetic characteristics supported the description of a new genus and species with the type species as Pseudo-chroococcus couteii. The high taxonomic diversity in the muds of the thermal baths of Balaruc-Les-Bains along with literature reports of the potential for bioactive metabolite synthesis of these taxa allowed us to hypothesize that some of the metabolites produced by these strains could contribute to the therapeutic properties of the muds from Thermes de Balaruc-Les-Bains.

scholarly journals Humihabitans oryzae gen. nov., sp. nov.

2007 ◽  
Vol 57 (9) ◽  
pp. 2163-2166 ◽  
Author(s):  
Akiko Kageyama ◽  
Yoko Takahashi ◽  
Satoshi Ōmura
Keyword(s):  
New Genus ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Mycolic Acids ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Paddy Field Soil ◽  
Diamino Acid ◽  
Aerobic Organism

Strain KV-657T was isolated from a paddy field soil sample collected in Japan using GPM agar plates supplemented with catalase. The strain was a Gram-positive, aerobic organism that formed branching hyphae with ll-diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major menaquinone was MK-8(H4). Mycolic acids were not detected. The G+C content of the DNA was 70 mol%. 16S rRNA gene sequence analysis revealed that this strain is closely related to Intrasporangium calvum DSM 43043T, with a similarity of 97.6 %. Based on the morphological, biochemical and chemotaxonomic properties of this strain and phylogenetic analysis, it was concluded that this isolate represents a new genus and species in the family Intrasporangiaceae, for which the name Humihabitans oryzae gen. nov., sp. nov. is proposed. The type strain of Humihabitans oryzae is KV-657T (=NRRL B-24470T =NBRC 101802T).

scholarly journals Nitritalea halalkaliphila gen. nov., sp. nov., an alkaliphilic bacterium of the family ‘Cyclobacteriaceae’, phylum Bacteroidetes

2010 ◽  
Vol 60 (10) ◽  
pp. 2320-2325 ◽  
Author(s):  
P. Anil Kumar ◽  
T. N. R. Srinivas ◽  
P. Pavan Kumar ◽  
S. Madhu ◽  
S. Shivaji
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Lonar Lake ◽  
Alkaliphilic Bacterium ◽  
Sequence Similarities

A novel Gram-negative, rod-shaped, non-motile bacterium, designated strain LW7T, was isolated from a water sample collected at a depth of 4.5 m from Lonar Lake in Buldhana district, Maharastra, India. The cell suspension was dark-reddish orange due to the presence of carotenoids. The fatty acids were dominated by large amounts of iso-C15 : 0 (59.6 %) and iso-C17 : 0 3-OH (8.9 %). Strain LW7T contained MK-4 and MK-5 as the major respiratory quinones and phosphatidylglycerol and phosphatidylethanolamine as the major phospholipids. 16S rRNA gene sequence analysis indicated that Belliella baltica, a member of family ‘Cyclobacteriaceae’ (phylum Bacteroidetes), is the closest related species, with a sequence similarity of 94.0 % to the type strain. Other members of the family ‘Cyclobacteriaceae’ had sequence similarities of <93.3 %. Based on the above-mentioned phenotypic and phylogenetic characteristics, strain LW7T is proposed as a representative of a new genus and species, Nitritalea halalkaliphila gen. nov., sp. nov. The type strain of Nitritalea halalkaliphila is LW7T (=CCUG 57665T =JCM 15946T =NCCB 100279T). The genomic DNA G+C of strain LW7T is 49 mol%.

scholarly journals Emydomyces testavorans, a New Genus and Species of Onygenalean Fungus Isolated from Shell Lesions of Freshwater Aquatic Turtles

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Daniel B. Woodburn ◽  
Andrew N. Miller ◽  
Matthew C. Allender ◽  
Carol W. Maddox ◽  
Karen A. Terio
Keyword(s):  
New Genus ◽  
Diverse Group ◽  
New Genus And Species ◽  
Emerging Pathogens ◽  
Genetic Characteristics ◽  
Content Type ◽  
Tissue Sections ◽  
Reptile Species ◽  
Negative Impacts ◽  
Multigene Phylogenetic Analysis

ABSTRACTThe fungal orderOnygenalesincludes many pathogens of humans and animals, and recent studies have shown some onygenalean fungi to be significant emerging pathogens of reptiles. Although many of these fungi have similar morphological features in histologic tissue sections, recent molecular analyses have revealed a genetically complex and diverse group of reptile pathogens comprising several genera, most notablyNannizziopsis,Ophidiomyces, andParanannizziopsis. Infections by members of these genera have been previously reported in a variety of reptile species, including crocodilians, lizards, snakes, and tuataras, with negative impacts on conservation efforts for some reptiles. Despite the well-documented pathogenicity of these fungi in all other extant reptile lineages, infection has not yet been reported in aquatic turtles. In this study, we report the isolation of an onygenalean fungus associated with shell lesions in freshwater aquatic turtles. The morphologic and genetic characteristics of multiple isolates (n= 21) are described and illustrated. Based on these features and results of a multigene phylogenetic analysis, a new genus and species,Emydomyces testavorans, are proposed for these fungi isolated from turtle shell lesions.

A new genus and species of primary freshwater crab and a new species of Artopotamon Dai & Chen, 1985 (Crustacea, Brachyura, Potamidae) from western Yunnan, China

Zootaxa ◽  
2018 ◽  
Vol 4422 (1) ◽  
pp. 115 ◽  
Author(s):  
KELIN CHU ◽  
PENGFEI WANG ◽  
HONGYING SUN
Keyword(s):  
New Species ◽  
New Genus ◽  
Molecular Data ◽  
Systematic Position ◽  
Rrna Gene ◽  
Freshwater Crab ◽  
New Genus And Species ◽  
Type Specimens ◽  
Western Yunnan ◽  
A New Species

A new genus and species belonging to the primary freshwater crab family Potamidae, and a new species of the genus Artopotamon Dai & Chen, 1985, are described on the basis of remarkable G1and female gonopore characters. Semicirculara lincangensis gen. & sp. nov. and Artopotamon latopeos sp. nov. were collected from southwestern and northwestern Yunnan Province, China, respectively. All type specimens are deposited in Nanjing Normal University in Nanjing and the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing. Mitochondrial molecular data, from the partial sequence of the mitochondrial 16S rRNA gene, are also shown to support the distinct systematic position of the new taxa. 

scholarly journals A new cistecephalid (Dicynodontia: Emydopoidea) from the Upper Permian of Malawi

2021 ◽  
Author(s):  
Ricardo Araújo ◽  
Zanildo Macungo ◽  
Vincent Fernadez ◽  
Elizabeth G. Chindebvu ◽  
Louis L. Jacobs
Keyword(s):  
New Genus ◽  
Taxonomic Diversity ◽  
Upper Permian ◽  
Micro Computed Tomography ◽  
New Genus And Species ◽  
Basal Clade ◽  
Cranial Osteology ◽  
First Time ◽  
Arteries And Veins ◽  
The Brain

Cistecephalidae is a relatively basal clade of dicynodonts, well-nested within emydopoids, and known to have multiple adaptations to a fossorial lifestyle. In recent years cistecephalid taxonomic diversity has been progressively increasing and important insights into the osteology, soft-tissue anatomy, and paleobiology of the clade have improved considerably. Here we describe a new genus and species of a cistecephalid from the Chiweta Beds (Northern Malawi, Wuchiapingian), a yet inadequately sampled area of Karoo sediments. Due to the minute dimensions and delicate nature of specimen PK-16-1, we have utilized, propagation phase-contrast synchrotron X-ray micro-computed tomography, revealing conspicuous contrast between rock matrix and bone and allowing reconstruction of its cranial osteology in great detail. PK-16-1 is here recognized as a new genus and species based on various autapomorphies, such as the presence of an atrophied preparietal and the presence of convergent crests along the pila antoticae. We performed a phylogenetic analysis in which we recovered PK-16-1 in a clade with Kembawacela and Cistecephalus united, among other characters, by a strongly embayed rostrum notch. Furthermore, the anatomical resolution obtained allowed us to reconstruct the osseous labyrinth, the paths for several nerve branches, arteries and veins for the first time in cistecephalids, and we develop new hypotheses for their paths and nomenclature for dicynodonts. We found remarkable resemblance in the brain endocast of PK-16-1 and another cistecephalid, Kawingasaurus, which is considerably distinct from other dicynodonts.

Spongiosarcinopsis terrestris gen. et sp. nov. (Chlorophyta, Chlorophyceae): a new genus of green algae from gray forest soil, Russia

Phytotaxa ◽  
2018 ◽  
Vol 376 (6) ◽  
pp. 291 ◽  
Author(s):  
ANNA TEMRALEEVA ◽  
SVETLANA MOSKALENKO ◽  
ELENA MINCHEVA ◽  
YURY BUKIN ◽  
MARIA SINETOVA
Keyword(s):  
Forest Soil ◽  
18S Rrna ◽  
New Genus ◽  
Gray Forest Soil ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Molecular Tools ◽  
New Genus And Species ◽  
Compensatory Base Changes ◽  
Percent Similarity

The diversity of soil green microalgae (Chlorophyta) is generally poorly known and likely underestimated because of their morphological poverty and crypticity. The use of molecular tools has revolutionized algal diversity research, including the discovery of numerous new taxa. On the basis of light microscopy, we isolated Chlorochytrium-like strain ACSSI 023 from gray forest soil (Moscow region, Russia), but with spongy rather than network chloroplast. This strain was evaluated by means of 18S rRNA gene and ITS2 sequences analyses and ultrastructural observations. Morphology, comparing the ITS2 secondary structure, compensatory base changes, intron lacking and percent similarity of the 18S rRNA gene of the isolated strain allowed us to propose a new genus and species for it, Spongiosarcinopsis terrestris gen. et sp. nov. The genus forms a separate independent branch within the Stephanosphaerinia clade (Chlorophyta, Chlorophyceae) with type species—Spongiosarcinopsis terrestris ACSSI 023.

scholarly journals Limnomma, a new genus of Ommatidae from the Middle Jurassic Daohugou beds (Coleoptera, Archostemata)

2021 ◽  
Vol 68 (2) ◽  
pp. 299-308
Author(s):  
Yan-Da Li ◽  
Erik Tihelka ◽  
Hong Pang ◽  
Di-Ying Huang ◽  
Chen‑Yang Cai
Keyword(s):  
Middle Jurassic ◽  
Northeast China ◽  
New Genus ◽  
Taxonomic Diversity ◽  
New Taxon ◽  
Morphological Disparity ◽  
High Diversity

The relictual archostematan beetle family Ommatidae attained high diversity during the Mesozoic. Despite their once high taxonomic diversity and morphological disparity, many Mesozoic ommatid taxa remain poorly understood, partly due to limited preservation. Here we report an exceptionally well-preserved fossil, which we describe as a new ommatid genus and species, Limnomma daohugouensegen. et sp. nov., from the mid-Jurassic Daohugou Lagerstätte in Northeast China. The new genus can be most easily distinguished from other ommatids by the presence of a circular non-tuberculate region on ventrite 5. The new taxon is discussed in relation to the classification of the Mesozoic genera Brochocoleus and Burmocoleus.

scholarly journals Characterization of the mycobiome of the seagrass, Zostera marina, reveals putative associations with marine chytrids

2019 ◽  
Author(s):  
Cassandra L. Ettinger ◽  
Jonathan A. Eisen
Keyword(s):  
Zostera Marina ◽  
Marine Fungi ◽  
Its Region ◽  
Taxonomic Diversity ◽  
Its2 Region ◽  
Rrna Gene ◽  
Sequence Variant ◽  
28S Rrna ◽  
Taxonomic Assignment ◽  
28S Rrna Gene

AbstractSeagrasses are globally distributed marine flowering plants that are foundation species in coastal ecosystems. Seagrass beds play essential roles as habitats and hatcheries, in nutrient cycling and in protecting the coastline from erosion. Although many studies have focused on seagrass ecology, only a limited number have investigated their associated fungi. In terrestrial systems, fungi can have beneficial and detrimental effects on plant fitness. However, not much is known about marine fungi and even less is known about seagrass associated fungi. Here we used culture-independent sequencing of the ribosomal internal transcribed spacer (ITS) region to characterize the taxonomic diversity of fungi associated with the seagrass, Zostera marina. We sampled from two Z. marina beds in Bodega Bay over three time points to investigate fungal diversity within and between plants. Our results indicate that there are many fungal taxa for which a taxonomic assignment cannot be made living on and inside Z. marina leaves, roots and rhizomes and that these plant tissues harbor distinct fungal communities. The most prevalent ITS amplicon sequence variant (ASV) associated with Z. marina leaves was classified as fungal, but could not initially be assigned to a fungal phylum. We then used PCR with a primer targeting unique regions of the ITS2 region of this ASV and an existing primer for the fungal 28S rRNA gene to amplify part of the 28S rRNA gene region and link it to this ASV. Sequencing and phylogenetic analysis of the resulting partial 28S rRNA gene revealed that the organism that this ASV comes from is a member of Novel Clade SW-I in the order Lobulomycetales in the phylum Chytridiomycota. This clade includes known parasites of freshwater diatoms and algae and it is possible this chytrid is directly infecting Z. marina leaf tissues. This work highlights a need for further studies focusing on marine fungi and the potential importance of these understudied communities to the larger seagrass ecosystem.

scholarly journals Polyphasic characterization of Trichocoleus desertorum sp. nov. (Pseudanabaenales, Cyanobacteria) from desert soils and phylogenetic placement of the genus Trichocoleus

Phytotaxa ◽  
2014 ◽  
Vol 163 (5) ◽  
pp. 241 ◽  
Author(s):  
Radka Muhlsteinova ◽  
Jeffrey R. Johansen ◽  
Nicole Pietrasiak ◽  
Michael P. Martin ◽  
Karina Osorio-Santos ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Its Region ◽  
Taxonomic Diversity ◽  
Morphological Description ◽  
Rrna Gene ◽  
New Taxon ◽  
Desert Soils ◽  
Phylogenetic Placement ◽  
Polyphasic Characterization

Little is known about the taxonomic diversity of cyanobacteria in deserts, despite their important ecological roles in these ecosystems. In this study, cyanobacterial strains from the Atacama, Colorado, and Mojave Deserts were isolated and characterized using molecular, morphological, and ecological information. Phylogenetic placement of these strains was revealed through Bayesian and parsimony-based phylogenetic analyses utilizing sequences of the 16S rRNA gene and the associated 16S–23S ITS region. Based on the combined evidence of this polyphasic approach, a new species from desert soils morphologically corresponding to the genus Trichocoleus was described. Trichocoleus desertorum sp. nov. Mühlsteinová, Johansen et Pietrasiak was used to obtain a phylogenetic reference point for Trichocoleus, a genus so far characterized by morphological description only. Through characterization of this new taxon in desert soils we hope to contribute to the general understanding of cyanobacterial diversity in extreme arid habitats.

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