scholarly journals Characterization of cyanobacteria isolated from thermal muds of Balaruc- Les-Bains (France) and description of a new genus and species Pseudochroococcus couteii

2020 ◽  
Author(s):  
Charlotte Duval ◽  
Sahima Hamlaoui ◽  
Berenice Piquet ◽  
Geraldine Toutirais ◽  
Claude Yepremian ◽  
...  
Keyword(s):  
New Genus ◽  
Its Region ◽  
Taxonomic Diversity ◽  
Ecological Niches ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Genetic Characteristics ◽  
Retention Basin ◽  
Metabolite Synthesis

Cyanobacteria are able to synthesize a high diversity of natural compounds that account for their success in the colonization of a variety of ecological niches. Many of them have beneficial properties. The mud from the thermal baths of Balaruc-Les-Bains, one of the oldest thermal baths in France, has long been recognized as a healing treatment for arthro-rheumatic diseases. To characterize the cyanobacteria living in these muds and the metabolites they potentially produce, several strains were isolated from the water column and biofilms of the retention basin and analyzed using a polyphasic approach. Morphological, ultrastructural and molecular (16S rRNA gene and 16S-23S ITS region sequencing) methods were employed to identify nine cyanobacterial strains belonging to the orders Chroococcales, Synechococcales, Oscillatoriales and Nostocales. The combination of morphological and genetic characteristics supported the description of a new genus and species with the type species as Pseudo-chroococcus couteii. The high taxonomic diversity in the muds of the thermal baths of Balaruc-Les-Bains along with literature reports of the potential for bioactive metabolite synthesis of these taxa allowed us to hypothesize that some of the metabolites produced by these strains could contribute to the therapeutic properties of the muds from Thermes de Balaruc-Les-Bains.

scholarly journals Diversity of cyanobacteria from thermal muds (Balaruc-Les-Bains, France) with the description of Pseudochroococcus coutei gen. nov., sp. nov.

FEMS Microbes ◽  
2021 ◽  
Vol 2 ◽  
Author(s):  
C Duval ◽  
S Hamlaoui ◽  
B Piquet ◽  
G Toutirais ◽  
C Yéprémian ◽  
...  
Keyword(s):  
New Genus ◽  
Its Region ◽  
Taxonomic Diversity ◽  
Ecological Niches ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Genetic Characteristics ◽  
Retention Basin ◽  
Therapeutic Properties ◽  
High Diversity

ABSTRACT Cyanobacteria are able to synthesize a high diversity of natural compounds that account for their success in the colonization of a variety of ecological niches. Many of them have beneficial properties. The mud from the thermal baths of Balaruc-Les-Bains, one of the oldest thermal baths in France, has long been recognized as a healing treatment for arthro-rheumatic diseases. To characterize the cyanobacteria living in these muds, several strains were isolated from the water column and biofilms of the retention basin and analyzed using a polyphasic approach. Morphological, ultrastructural and molecular (16S rRNA gene and 16S-23S ITS region sequencing) methods were employed to identify nine cyanobacterial strains belonging to the orders Chroococcales, Synechococcales, Oscillatoriales and Nostocales. The combination of morphological and genetic characteristics supported the description of a new genus and species with the type species as Pseudochroococcus coutei. The taxonomic diversity in the muds from Thermes de Balaruc-Les-Bains appears higher than previously documented, providing new candidate taxa for their observed therapeutic properties.

scholarly journals Polyphasic characterization of Trichocoleus desertorum sp. nov. (Pseudanabaenales, Cyanobacteria) from desert soils and phylogenetic placement of the genus Trichocoleus

Phytotaxa ◽  
2014 ◽  
Vol 163 (5) ◽  
pp. 241 ◽  
Author(s):  
Radka Muhlsteinova ◽  
Jeffrey R. Johansen ◽  
Nicole Pietrasiak ◽  
Michael P. Martin ◽  
Karina Osorio-Santos ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Its Region ◽  
Taxonomic Diversity ◽  
Morphological Description ◽  
Rrna Gene ◽  
New Taxon ◽  
Desert Soils ◽  
Phylogenetic Placement ◽  
Polyphasic Characterization

Little is known about the taxonomic diversity of cyanobacteria in deserts, despite their important ecological roles in these ecosystems. In this study, cyanobacterial strains from the Atacama, Colorado, and Mojave Deserts were isolated and characterized using molecular, morphological, and ecological information. Phylogenetic placement of these strains was revealed through Bayesian and parsimony-based phylogenetic analyses utilizing sequences of the 16S rRNA gene and the associated 16S–23S ITS region. Based on the combined evidence of this polyphasic approach, a new species from desert soils morphologically corresponding to the genus Trichocoleus was described. Trichocoleus desertorum sp. nov. Mühlsteinová, Johansen et Pietrasiak was used to obtain a phylogenetic reference point for Trichocoleus, a genus so far characterized by morphological description only. Through characterization of this new taxon in desert soils we hope to contribute to the general understanding of cyanobacterial diversity in extreme arid habitats.

scholarly journals Humihabitans oryzae gen. nov., sp. nov.

2007 ◽  
Vol 57 (9) ◽  
pp. 2163-2166 ◽  
Author(s):  
Akiko Kageyama ◽  
Yoko Takahashi ◽  
Satoshi Ōmura
Keyword(s):  
New Genus ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Mycolic Acids ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Paddy Field Soil ◽  
Diamino Acid ◽  
Aerobic Organism

Strain KV-657T was isolated from a paddy field soil sample collected in Japan using GPM agar plates supplemented with catalase. The strain was a Gram-positive, aerobic organism that formed branching hyphae with ll-diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major menaquinone was MK-8(H4). Mycolic acids were not detected. The G+C content of the DNA was 70 mol%. 16S rRNA gene sequence analysis revealed that this strain is closely related to Intrasporangium calvum DSM 43043T, with a similarity of 97.6 %. Based on the morphological, biochemical and chemotaxonomic properties of this strain and phylogenetic analysis, it was concluded that this isolate represents a new genus and species in the family Intrasporangiaceae, for which the name Humihabitans oryzae gen. nov., sp. nov. is proposed. The type strain of Humihabitans oryzae is KV-657T (=NRRL B-24470T =NBRC 101802T).

scholarly journals Genetic characterization of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, southwestern of China-Zoonotic potential

2021 ◽  
Author(s):  
Shanyu Chen ◽  
Wangyu Meng ◽  
Ziyao Zhou ◽  
Lei Deng ◽  
Xiaogang Shi ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Human Infection ◽  
Rrna Gene ◽  
Zoonotic Potential ◽  
Wild Animals ◽  
Genetic Characteristics ◽  
Natural Reserve ◽  
Faecal Samples ◽  
Wide Range

Abstract Background Blastocystis, a highly prevalent eukaryotic parasite, has been identified in a wide range of hosts, including humans, domestic and wild animals. Many animals are potential sources of Blastocystis infection for humans, while few information about the prevalence of Blastocystis in wild animals have being documented. Therefore, the present study was designed to investigate the prevalence and subtypes of Blastocystis in wild animals of Sichuan Wolong National Natural Reserve, southwestern of China, so as to assess the zoonotic potential of these animals. Methods A total of 300 faecal samples were collected from 27 wildlife species in three areas of Sichuan Wolong National Natural Reserve in southwestern China. The subtype (ST) genetic characteristics and prevalence of Blastocystis were determined by PCR amplification of the barcode region (a fragment of ∼600 bp) of the SSU rRNA gene, and phylogenetic analysis were further performed to determine the genetic characteristics of Blastocystis subtypes. Results 30 of 300 faecal samples (10.0%) were Blastocystis-positive. The highest prevalence of Blastocystis was found in Yinchanggou (18.3%), which was significantly higher than that in Niutoushan (7.5%), and Genda (5.5%) (P < 0.05). Specifically, the highest prevalence of Blastocystis was found in primates (20.0%, 1/5), followed by rodentia 14.3% (1/7), artiodactyla 13.1% (26/198), carnivora 2.3% (2/87), galliformes 0% (0/3). Sequence analysis showed 5 subtypes (ST1, ST3, ST5, ST13, and ST14), with ST13 and ST14 as the predominant subtype (33.3%, 10/30), followed by ST1 (20.0%, 6/30). Conclusions To the best of our knowledge, this is the first molecular investigation on Blastocystis infection in wild animals in southwestern of China. ST1, ST3, and ST5 were identified in both humans and wild animals, suggesting that these wild animals may be potential reservoirs of Blastocystis for human infection.

scholarly journals Nitritalea halalkaliphila gen. nov., sp. nov., an alkaliphilic bacterium of the family ‘Cyclobacteriaceae’, phylum Bacteroidetes

2010 ◽  
Vol 60 (10) ◽  
pp. 2320-2325 ◽  
Author(s):  
P. Anil Kumar ◽  
T. N. R. Srinivas ◽  
P. Pavan Kumar ◽  
S. Madhu ◽  
S. Shivaji
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Lonar Lake ◽  
Alkaliphilic Bacterium ◽  
Sequence Similarities

A novel Gram-negative, rod-shaped, non-motile bacterium, designated strain LW7T, was isolated from a water sample collected at a depth of 4.5 m from Lonar Lake in Buldhana district, Maharastra, India. The cell suspension was dark-reddish orange due to the presence of carotenoids. The fatty acids were dominated by large amounts of iso-C15 : 0 (59.6 %) and iso-C17 : 0 3-OH (8.9 %). Strain LW7T contained MK-4 and MK-5 as the major respiratory quinones and phosphatidylglycerol and phosphatidylethanolamine as the major phospholipids. 16S rRNA gene sequence analysis indicated that Belliella baltica, a member of family ‘Cyclobacteriaceae’ (phylum Bacteroidetes), is the closest related species, with a sequence similarity of 94.0 % to the type strain. Other members of the family ‘Cyclobacteriaceae’ had sequence similarities of <93.3 %. Based on the above-mentioned phenotypic and phylogenetic characteristics, strain LW7T is proposed as a representative of a new genus and species, Nitritalea halalkaliphila gen. nov., sp. nov. The type strain of Nitritalea halalkaliphila is LW7T (=CCUG 57665T =JCM 15946T =NCCB 100279T). The genomic DNA G+C of strain LW7T is 49 mol%.

scholarly journals Emydomyces testavorans, a New Genus and Species of Onygenalean Fungus Isolated from Shell Lesions of Freshwater Aquatic Turtles

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Daniel B. Woodburn ◽  
Andrew N. Miller ◽  
Matthew C. Allender ◽  
Carol W. Maddox ◽  
Karen A. Terio
Keyword(s):  
New Genus ◽  
Diverse Group ◽  
New Genus And Species ◽  
Emerging Pathogens ◽  
Genetic Characteristics ◽  
Content Type ◽  
Tissue Sections ◽  
Reptile Species ◽  
Negative Impacts ◽  
Multigene Phylogenetic Analysis

ABSTRACTThe fungal orderOnygenalesincludes many pathogens of humans and animals, and recent studies have shown some onygenalean fungi to be significant emerging pathogens of reptiles. Although many of these fungi have similar morphological features in histologic tissue sections, recent molecular analyses have revealed a genetically complex and diverse group of reptile pathogens comprising several genera, most notablyNannizziopsis,Ophidiomyces, andParanannizziopsis. Infections by members of these genera have been previously reported in a variety of reptile species, including crocodilians, lizards, snakes, and tuataras, with negative impacts on conservation efforts for some reptiles. Despite the well-documented pathogenicity of these fungi in all other extant reptile lineages, infection has not yet been reported in aquatic turtles. In this study, we report the isolation of an onygenalean fungus associated with shell lesions in freshwater aquatic turtles. The morphologic and genetic characteristics of multiple isolates (n= 21) are described and illustrated. Based on these features and results of a multigene phylogenetic analysis, a new genus and species,Emydomyces testavorans, are proposed for these fungi isolated from turtle shell lesions.

scholarly journals Mucispirillum schaedleri gen. nov., sp. nov., a spiral-shaped bacterium colonizing the mucus layer of the gastrointestinal tract of laboratory rodents

2005 ◽  
Vol 55 (3) ◽  
pp. 1199-1204 ◽  
Author(s):  
Bronwyn R. Robertson ◽  
Jani L. O'Rourke ◽  
Brett A. Neilan ◽  
Peter Vandamme ◽  
Stephen L. W. On ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
New Genus ◽  
Bacterial Species ◽  
Mucus Layer ◽  
Bacterial Isolates ◽  
New Genus And Species ◽  
Laboratory Rodents ◽  
Distinct Lineage ◽  
High Level

The mammalian gastrointestinal tract is covered by a layer of mucus that can harbour a range of bacterial species specifically adapted to colonize this ecological niche. Examination of 110 bacterial isolates cultivated from the gastrointestinal tract of 23 mice revealed the presence of a subgroup of 30 isolates that did not correspond genetically with genera commonly associated with this site, i.e. members of the ε-Proteobacteria such as Helicobacter and Campylobacter species. Instead this group of isolates was found to lie within the phylum Deferribacteres, a completely distinct lineage in the domain Bacteria. There was a high level of consensus in results obtained from the phenotypic and genotypic characterization of a number of the isolates, which showed they were distinct from other members of the Deferribacteres. As such, they are proposed to constitute a new genus and species, Mucispirillum schaedleri gen. nov., sp. nov. These organisms are anaerobic, Gram-negative, spiral-shaped rods with bipolar flagella. The type strain is HRI I17T (=ATCC BAA-1009T=ACM 5223T).

A new genus and species of primary freshwater crab and a new species of Artopotamon Dai & Chen, 1985 (Crustacea, Brachyura, Potamidae) from western Yunnan, China

Zootaxa ◽  
2018 ◽  
Vol 4422 (1) ◽  
pp. 115 ◽  
Author(s):  
KELIN CHU ◽  
PENGFEI WANG ◽  
HONGYING SUN
Keyword(s):  
New Species ◽  
New Genus ◽  
Molecular Data ◽  
Systematic Position ◽  
Rrna Gene ◽  
Freshwater Crab ◽  
New Genus And Species ◽  
Type Specimens ◽  
Western Yunnan ◽  
A New Species

A new genus and species belonging to the primary freshwater crab family Potamidae, and a new species of the genus Artopotamon Dai & Chen, 1985, are described on the basis of remarkable G1and female gonopore characters. Semicirculara lincangensis gen. & sp. nov. and Artopotamon latopeos sp. nov. were collected from southwestern and northwestern Yunnan Province, China, respectively. All type specimens are deposited in Nanjing Normal University in Nanjing and the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing. Mitochondrial molecular data, from the partial sequence of the mitochondrial 16S rRNA gene, are also shown to support the distinct systematic position of the new taxa. 

Isolation and characterization of cellulolytic anaerobic fungi and associated mycoplasmas from the rumen of a steer fed a roughage diet

1990 ◽  
Vol 36 (7) ◽  
pp. 513-517 ◽  
Author(s):  
H. Kudo ◽  
K. D. Jakober ◽  
R. C. Phillippe ◽  
K.-J. Cheng ◽  
D. J. S. Barr ◽  
...  
Keyword(s):  
Key Words ◽  
New Genus ◽  
Anaerobic Fungi ◽  
Ecological Role ◽  
New Genus And Species ◽  
Fermentation Products ◽  
Isolation And Characterization ◽  
Neocallimastix Patriciarum

The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation patterns were very similar to those of rumen fungi isolated in other countries. Mycoplasmas were found to be associated with 7 of the 12 isolates. The ecological role of the association of the mycoplasmas with rumen fungi is still unknown. Key words: Orpinomyces bovis, Piromyces communis, Neocallimastix patriciarum.

scholarly journals A new cistecephalid (Dicynodontia: Emydopoidea) from the Upper Permian of Malawi

2021 ◽  
Author(s):  
Ricardo Araújo ◽  
Zanildo Macungo ◽  
Vincent Fernadez ◽  
Elizabeth G. Chindebvu ◽  
Louis L. Jacobs
Keyword(s):  
New Genus ◽  
Taxonomic Diversity ◽  
Upper Permian ◽  
Micro Computed Tomography ◽  
New Genus And Species ◽  
Basal Clade ◽  
Cranial Osteology ◽  
First Time ◽  
Arteries And Veins ◽  
The Brain

Cistecephalidae is a relatively basal clade of dicynodonts, well-nested within emydopoids, and known to have multiple adaptations to a fossorial lifestyle. In recent years cistecephalid taxonomic diversity has been progressively increasing and important insights into the osteology, soft-tissue anatomy, and paleobiology of the clade have improved considerably. Here we describe a new genus and species of a cistecephalid from the Chiweta Beds (Northern Malawi, Wuchiapingian), a yet inadequately sampled area of Karoo sediments. Due to the minute dimensions and delicate nature of specimen PK-16-1, we have utilized, propagation phase-contrast synchrotron X-ray micro-computed tomography, revealing conspicuous contrast between rock matrix and bone and allowing reconstruction of its cranial osteology in great detail. PK-16-1 is here recognized as a new genus and species based on various autapomorphies, such as the presence of an atrophied preparietal and the presence of convergent crests along the pila antoticae. We performed a phylogenetic analysis in which we recovered PK-16-1 in a clade with Kembawacela and Cistecephalus united, among other characters, by a strongly embayed rostrum notch. Furthermore, the anatomical resolution obtained allowed us to reconstruct the osseous labyrinth, the paths for several nerve branches, arteries and veins for the first time in cistecephalids, and we develop new hypotheses for their paths and nomenclature for dicynodonts. We found remarkable resemblance in the brain endocast of PK-16-1 and another cistecephalid, Kawingasaurus, which is considerably distinct from other dicynodonts.

Sign in / Sign up

Export Citation Format

Share Document