Neutrophil and monocyte receptor expression in patients with sepsis: implications for diagnosis and prognosis of sepsis

2019 ◽  
Vol 77 (6) ◽  
Author(s):  
Mariam Onsy F Hanna ◽  
Asmaa M Abdelhameed ◽  
Amany A Abou-Elalla ◽  
Reem M Hassan ◽  
Inas Kostandi
Keyword(s):  
Mortality Risk ◽  
Clinical Syndrome ◽  
Receptor Expression ◽  
C Reactive Protein ◽  
Surface Receptors ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Neutrophil Cd64 ◽  
Combination Approach ◽  
Insight Into

ABSTRACT Understanding the complex immune responses in sepsis is crucial to provide insight into the clinical syndrome. We evaluated the changes in the surface receptors of the cells of innate immunity, neutrophils and monocytes, in patients with sepsis. Since sepsis remains a clinical challenge, we aimed to assess the significance of altered receptor expression in diagnosis and prognosis. Critically ill patients with sepsis (n=31) were investigated for the expression of receptors for IgG heavy chain CD64 and CD16 on neutrophils and CD64 and the lipopolysaccharide receptor CD14 on monocytes by flow cytometry and compared to 23 patients with no sepsis. Patients with sepsis had increased expression of neutrophil CD64. Neutrophil CD64 was specific for discriminating patients with sepsis but showed weak sensitivity. When integrated in a scoring system, neutrophil CD64 in combination with C-reactive protein (CRP) and SOFA score showed a diagnostic accuracy of 0.93 for sepsis and significantly predicted increased mortality risk. While neutrophil CD16 did not discriminate for sepsis, decreased expression was associated with increased mortality risk. In contrast, monocyte CD64 and CD14 expression was unaltered in sepsis and was not associated with mortality risk. Our study demonstrates that unlike monocytes, neutrophil receptor expression is altered in patients with sepsis receiving intensive care. It is promising to apply a combination approach to diagnose sepsis especially in time-limited conditions.

C reactive protein contributes to the early prediction of 90-day mortality risk in acutely decompensated cirrhotic patients

2017 ◽  
Vol 66 (1) ◽  
pp. S126-S127
Author(s):  
A. Clemente ◽  
M. Romero ◽  
C. Martínez ◽  
A. Bueno ◽  
M. Antona ◽  
...  
Keyword(s):  
Mortality Risk ◽  
Early Prediction ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cirrhotic Patients

Abstract 5393: Increased C-Reactive Protein Expression Exacerbates Left Ventricular Dysfunction and Remodeling after Myocardial Infarction

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Toshiyuki Takahashi ◽  
Toshihisa Anzai ◽  
Hidehiro Kaneko ◽  
Atsushi Anzai ◽  
Yoshinori Mano ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Angiotensin Ii ◽  
Left Ventricular ◽  
Receptor Expression ◽  
Border Zone ◽  
Histological Evaluation ◽  
Control Group ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Lv Remodeling

We have previously reported that elevated serum C-reactive protein (CRP) level after acute myocardial infarction (MI) is associated with adverse outcomes including cardiac rupture, left ventricular (LV) remodeling and cardiac death. Recent experimental studies have shown that CRP per se has some biological properties including proinflammatory and proapoptotic effects, suggesting a pathogenetic role of CRP in the remodeling process after MI. We tested the hypothesis that increased CRP expression would exacerbate adverse LV remodeling after MI through some deleterious effects of CRP. Transgenic mice with human CRP expression (CRP-Tg) and their nontransgenic littermates (Control) underwent proximal ligation of the left coronary artery. Despite increased serum CRP level and cardiac CRP expression in CRP-Tg mice, there was no difference in phenotype between CRP-Tg and control mice before MI. Mortality at five weeks after MI was not different between groups (CRP-Tg: 49%, n=35; Control: 38%, n=40, P =0.28). Five weeks after MI, echocardiography showed that CRP-Tg mice had more LV dilation (LVEDD, CRP-Tg: 5.8 ± 0.1 mm, n=14; Control: 5.2 ± 0.1 mm, n=17, P =0.002) and worse LV function (EF, CRP-Tg: 13 ± 2%, n=14; Control: 19 ± 1%, n=17, P =0.01). Hemodynamic studies indicated that LV +dP/dt (CRP-Tg: 2,947 ± 480 mmHg/s, n=9; Control: 3,788 ± 656 mmHg/s, n=10, P =0.02) and -dP/dt (CRP-Tg: −2,230 ± 48 mmHg/s, n=9; Control: −2,890 ± 161 mmHg/s, n=10, P =0.003) were lower in the CRP-Tg group than in the Control group, although infarct size was comparable. Histological evaluation at one week after MI showed a higher rate of apoptosis in the border zone of infarcted hearts from CRP-Tg mice (CRP-Tg: 1,434 ± 322 per 10 5 nuclei; Control: 596 ± 112 per 10 5 nuclei, n=6 for each, P =0.03). Quantitative RT-PCR showed that angiotensin II type 1a receptor and interleukin-6 were upregulated in viable LV samples from CRP-Tg mice compared with controls. Increased CRP expression exacerbates LV dysfunction and remodeling after MI, associated with increased apoptotic rates, increased angiotensin II receptor expression and exaggerated inflammatory response.

scholarly journals High sensitivity C-reactive protein: Potential biomarker of inflammation in acute mTBI

Neurology ◽  
2018 ◽  
Vol 91 (23 Supplement 1) ◽  
pp. S18.1-S18
Author(s):  
Teena Shetty ◽  
Cogsil Taylor ◽  
Aashka Dalal ◽  
Kristin Halvorsen ◽  
Kelianne Cummings ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Binary Logistic Regression ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Hscrp Level ◽  
Reactive Protein ◽  
Blood Biomarker ◽  
Diagnosis And Prognosis ◽  
Potential Factors ◽  
Delayed Time

ObjectiveThis study investigated the utility of high-sensitivity C-Reactive Protein (hsCRP) as a blood biomarker for mild traumatic brain injury (mTBI).BackgroundValidation of a blood biomarker panel will greatly improve mTBI diagnosis and prognosis. hsCRP has been validated as a sensitive biomarker for inflammation. Previous studies have established relationships between CRP levels and TBI, but the utilization of hsCRP levels in assessing mTBI requires further exploration.MethodsRetrospective chart review collected hsCRP values in acute mTBI patients seen within 30 days of injury. Patients with any comorbid diagnosis known to cause elevation of inflammatory proteins were excluded. Continuous hsCRP levels were transformed into quartiles: <0.200 mg/L for Quartile 1 (Q1); 0.200–0.415 mg/L for Quartile 2 (Q2); 0.415–1.100 mg/L for Quartile 3 (Q3); and ≥1.100 mg/L for Quartile 4 (Q4). Multivariable binary logistic regression modeling identified potential factors for elevated hsCRP at first visit. Cox regression analysis identified potential factors for delayed time to recovery.ResultsThree hundred twelve injuries in 311 patients were reviewed (mean age 21 ± 12 years, 53% female). Mean hsCRP was elevated patients who presented within 2 days of injury and was found to significantly decrease between first visit and 4 weeks post-injury (p = 0.016). Initial hsCRP level was positively correlated with age (r = 0.163, p = 0.004) and negatively associated with previous concussion history (p = 0.031). When analyzed as quartiles, patients in Q4 were more likely to have endorsed headache (p = 0.036) or fatigue (p = 0.030). Age significantly increased between quartiles (p = 0.013). Multivariable binary logistic regression showed that increased age (OR: 3.48) and patients presenting with headache (OR: 3.48) or fatigue (OR: 2.16) were significantly associated with increased risk of having an hsCRP level in Q4. Females (HR: 0.32) and increased age (HR: 0.95) were associated delayed time to recovery.ConclusionshsCRP may be a viable addition to acute and longitudinal biomarker panels for diagnosis and prognosis of mTBI.

scholarly journals Insight into infection-mediated prostate damage: Contrasting patterns of C-reactive protein and prostate-specific antigen levels during infection

The Prostate ◽  
2017 ◽  
Vol 77 (13) ◽  
pp. 1325-1334 ◽  
Author(s):  
Melissa Milbrandt ◽  
Anke C. Winter ◽  
Remington L. Nevin ◽  
Ratna Pakpahan ◽  
Gary Bradwin ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Insight Into

scholarly journals Procalcitonin Increase Is Associated with the Development of Critical Care-Acquired Infections in COVID-19 ARDS

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1425
Author(s):  
Owen Richards ◽  
Philip Pallmann ◽  
Charles King ◽  
Yusuf Cheema ◽  
Charlotte Killick ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Secondary Infection ◽  
Ventilator Associated Pneumonia ◽  
C Reactive Protein ◽  
Comparative Efficacy ◽  
Blood Cell Count ◽  
Single Centre ◽  
Secondary Bacterial Infection ◽  
Reactive Protein ◽  
Insight Into

Secondary bacterial infection in COVID-19 patients is associated with increased mortality and disproportionately affects critically ill patients. This single-centre retrospective observational study investigates the comparative efficacy of change in procalcitonin (PCT) and other commonly available biomarkers in revealing or predicting microbiologically proven secondary infection in critical COVID-19 patients. Adult patients admitted to an intensive care unit (ICU) with confirmed SARS-CoV-2 infection between 9 March 2020 and 5 June 2020 were recruited to the study. For daily biomarker and secondary infection, laboratory-confirmed bloodstream infection (LCBI) and ventilator-associated pneumonia/tracheobronchitis (VAP/VAT) data were collected. We observed a PCT rise in 53 (81.5%) of the patients, a C-reactive protein (CRP) rise in 55 (84.6%) and a white blood cell count (WBC) rise in 61 (93.8%). Secondary infection was confirmed in 33 (50.8%) of the patients. A PCT rise was present in 97.0% of patients with at least one confirmed VAP/VAT and/or LCBI event. CRP and WBC rises occurred in 93.9% and 97.0% of patients with confirmed VAP/VAT and/or LCBI, respectively. Logistic regression analysis found that, when including all biomarkers in the same model, there was a significant association between PCT rise and the occurrence of LCBI and/or VAP/VAT (OR = 14.86 95%CI: 2.20, 342.53; p = 0.021). Conversely, no statistically significant relationship was found between either a CRP rise (p = 0.167) or a WBC rise (p = 0.855) and the occurrence of VAP/VAT and/or LCBI. These findings provide a promising insight into the usefulness of PCT measurement in predicting the emergence of secondary bacterial infection in ICU.

scholarly journals Neutrophil count to albumin ratio as a new predictor of mortality in patients with COVID-19 ınfection

2020 ◽  
Vol 66 (suppl 2) ◽  
pp. 77-81 ◽  
Author(s):  
Ceyhun Varim ◽  
Selcuk Yaylaci ◽  
Taner Demirci ◽  
Tezcan Kaya ◽  
Ahmet Nalbant ◽  
...  
Keyword(s):  
Platelet Count ◽  
Neutrophil Count ◽  
Mortality Risk ◽  
Demographic Data ◽  
C Reactive Protein ◽  
Albumin Ratio ◽  
Reactive Protein ◽  
Severe Group ◽  
Group 2 ◽  
Group 1

SUMMARY BACKGROUND Coronavirus Disease 2019 is an acute inflammatory respiratory disease. It causes many changes in hemogram parameters. Low albumin levels are associated with mortality risk in hospitalized patients. The aim of the present study is to reveal the place of neutrophil count to albumin ratio in predicting mortality in patients with COVID-19. METHODS 144 patients, 65 females and 79 males, were included in the study. Patients were divided into 2 groups. Group 1 was the non-severe group (n:85), and Group 2 was severe (n:59). Demographic data, neutrophil, lymphocyte and platelet counts, albumin and C-reactive protein (CRP) levels were recorded. Neutrophil count to albumin ratio (NAR) was calculated by dividing the absolute neutrophil counts by the albumin levels. The NAR and levels of the two groups were then compared. RESULTS There were no significant differences in gender and platelet count (201 vs. 211 K/mL) between the groups (p>0,05). Ages (62.0 ± 14.3 vs 68.6 ± 12.2 years), albumin (33.1 vs 29.9 gr/L), CRP (33 vs 113 mg/l), neutrophil count (4 vs 7.24 K/mL), WBC counts (6.70 vs 8.50 K/mL), NAR values (113.5 vs 267.2) and number of Death (5 vs 33) were found to be statistically higher (p <0.001) in Group 2 than in Group 1. The NAR value of 201.5 showed mortality in all patients with COVID-19 to have 71.1% sensitivity and 71.7% specificity (AUC:0.736, 95% CI: 0.641-0.832, p<0.001) CONCLUSION The present study showed that NAR levels can be a cheap and simple marker for predicting mortality in patients with COVID-19.

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