scholarly journals Comparison of the Accuracy of Procalcitonin, Neutrophil CD64, and c-Reactive Protein for the Diagnosis and Prognosis of Septic Patients Who Received Antimicrobial Therapies

2020 ◽  
Author(s):  
F. Wang ◽  
L. Ran ◽  
C. Qian ◽  
Q. Li
Keyword(s):  
C Reactive Protein ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Neutrophil Cd64

Neutrophil and monocyte receptor expression in patients with sepsis: implications for diagnosis and prognosis of sepsis

2019 ◽  
Vol 77 (6) ◽  
Author(s):  
Mariam Onsy F Hanna ◽  
Asmaa M Abdelhameed ◽  
Amany A Abou-Elalla ◽  
Reem M Hassan ◽  
Inas Kostandi
Keyword(s):  
Mortality Risk ◽  
Clinical Syndrome ◽  
Receptor Expression ◽  
C Reactive Protein ◽  
Surface Receptors ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Neutrophil Cd64 ◽  
Combination Approach ◽  
Insight Into

ABSTRACT Understanding the complex immune responses in sepsis is crucial to provide insight into the clinical syndrome. We evaluated the changes in the surface receptors of the cells of innate immunity, neutrophils and monocytes, in patients with sepsis. Since sepsis remains a clinical challenge, we aimed to assess the significance of altered receptor expression in diagnosis and prognosis. Critically ill patients with sepsis (n=31) were investigated for the expression of receptors for IgG heavy chain CD64 and CD16 on neutrophils and CD64 and the lipopolysaccharide receptor CD14 on monocytes by flow cytometry and compared to 23 patients with no sepsis. Patients with sepsis had increased expression of neutrophil CD64. Neutrophil CD64 was specific for discriminating patients with sepsis but showed weak sensitivity. When integrated in a scoring system, neutrophil CD64 in combination with C-reactive protein (CRP) and SOFA score showed a diagnostic accuracy of 0.93 for sepsis and significantly predicted increased mortality risk. While neutrophil CD16 did not discriminate for sepsis, decreased expression was associated with increased mortality risk. In contrast, monocyte CD64 and CD14 expression was unaltered in sepsis and was not associated with mortality risk. Our study demonstrates that unlike monocytes, neutrophil receptor expression is altered in patients with sepsis receiving intensive care. It is promising to apply a combination approach to diagnose sepsis especially in time-limited conditions.

scholarly journals High sensitivity C-reactive protein: Potential biomarker of inflammation in acute mTBI

Neurology ◽  
2018 ◽  
Vol 91 (23 Supplement 1) ◽  
pp. S18.1-S18
Author(s):  
Teena Shetty ◽  
Cogsil Taylor ◽  
Aashka Dalal ◽  
Kristin Halvorsen ◽  
Kelianne Cummings ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Binary Logistic Regression ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Hscrp Level ◽  
Reactive Protein ◽  
Blood Biomarker ◽  
Diagnosis And Prognosis ◽  
Potential Factors ◽  
Delayed Time

ObjectiveThis study investigated the utility of high-sensitivity C-Reactive Protein (hsCRP) as a blood biomarker for mild traumatic brain injury (mTBI).BackgroundValidation of a blood biomarker panel will greatly improve mTBI diagnosis and prognosis. hsCRP has been validated as a sensitive biomarker for inflammation. Previous studies have established relationships between CRP levels and TBI, but the utilization of hsCRP levels in assessing mTBI requires further exploration.MethodsRetrospective chart review collected hsCRP values in acute mTBI patients seen within 30 days of injury. Patients with any comorbid diagnosis known to cause elevation of inflammatory proteins were excluded. Continuous hsCRP levels were transformed into quartiles: <0.200 mg/L for Quartile 1 (Q1); 0.200–0.415 mg/L for Quartile 2 (Q2); 0.415–1.100 mg/L for Quartile 3 (Q3); and ≥1.100 mg/L for Quartile 4 (Q4). Multivariable binary logistic regression modeling identified potential factors for elevated hsCRP at first visit. Cox regression analysis identified potential factors for delayed time to recovery.ResultsThree hundred twelve injuries in 311 patients were reviewed (mean age 21 ± 12 years, 53% female). Mean hsCRP was elevated patients who presented within 2 days of injury and was found to significantly decrease between first visit and 4 weeks post-injury (p = 0.016). Initial hsCRP level was positively correlated with age (r = 0.163, p = 0.004) and negatively associated with previous concussion history (p = 0.031). When analyzed as quartiles, patients in Q4 were more likely to have endorsed headache (p = 0.036) or fatigue (p = 0.030). Age significantly increased between quartiles (p = 0.013). Multivariable binary logistic regression showed that increased age (OR: 3.48) and patients presenting with headache (OR: 3.48) or fatigue (OR: 2.16) were significantly associated with increased risk of having an hsCRP level in Q4. Females (HR: 0.32) and increased age (HR: 0.95) were associated delayed time to recovery.ConclusionshsCRP may be a viable addition to acute and longitudinal biomarker panels for diagnosis and prognosis of mTBI.

scholarly journals Specific Receptors for the Chemokines CXCR2 and CXCR4 in Pancreatic Cancer

2020 ◽  
Vol 21 (17) ◽  
pp. 6193
Author(s):  
Ala Litman-Zawadzka ◽  
Marta Łukaszewicz-Zając ◽  
Mariusz Gryko ◽  
Agnieszka Kulczyńska-Przybik ◽  
Bogusław Kędra ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Tumor Marker ◽  
Immunological Methods ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Specific Receptors ◽  
New Biomarkers

Background: The mortality rate of pancreatic cancer (PC) is equal to its incidence and the majority of PC patients die within a few months of diagnosis. Therefore, a search for new biomarkers useful in the diagnosis and prognosis of PC is ongoing. Objectives: The aim of our study was to compare the utility of CXCR2 and CXCR4 in the diagnosis and prediction of PC with classical tumor marker (carcinoembryonic antigen, CEA) and marker of inflammation–C-reactive protein (CRP). Patients and Methods: The study comprised 64 subjects — 32 PC patients and 32 healthy volunteers. Serum concentrations of tested proteins were analysed using immunological methods. Results: Serum CXCR2 and CXCR4 concentrations, similarly to those of CEA and CRP, were significantly elevated in PC patients compared to healthy controls. Moreover, concentrations of CXCR4 were significantly correlated with CXCR2 and CRP levels, while CRP concentrations were correlated with CXCR2 and CEA levels. The diagnostic sensitivity and the predictive value for negative (PV−ve) results for CXCR4 were similar to those of CEA and higher than those of CXCR2 and CRP, while the area under the ROC curve (AUC) for CXCR4 was the highest among all tested proteins (CXCR2, CEA, CRP). Moreover, serum CXCR2 was found to be a significant predictor of PC risk. Conclusions: CXCR4 is a better candidate for a tumor marker than CXCR2 in the diagnosis of PC, while serum CXCR2 is a significant predictor of PC risk.

Neutrophil CD64 Is an Improved Indicator of Infection or Sepsis in Emergency Department Patients

2006 ◽  
Vol 130 (5) ◽  
pp. 654-661 ◽  
Author(s):  
Bruce H. Davis ◽  
Stephen H. Olsen ◽  
Ejaz Ahmad ◽  
Nancy C. Bigelow
Keyword(s):  
Emergency Department ◽  
Sedimentation Rate ◽  
Diagnostic Tests ◽  
Diagnostic Performance ◽  
Tertiary Care ◽  
Clinical Score ◽  
C Reactive Protein ◽  
Cd64 Expression ◽  
Reactive Protein ◽  
Neutrophil Cd64

Abstract Context.—Sepsis, affecting millions of individuals annually with an associated high mortality rate, is among the top 10 causes of death. In addition, improvements in diagnostic tests for detecting and monitoring sepsis and infection have been limited in the last 25 years. Neutrophil CD64 expression has been proposed as an improved diagnostic test for the evaluation of infection and sepsis. Objective.—To evaluate the diagnostic performance of a quantitative flow cytometric assay for leukocyte CD64 expression in comparison with the standard tests for infection/sepsis in an ambulatory care setting. Design.—Prospective analysis of 100 blood samples from patients from an emergency department setting in a 965-bed tertiary care suburban community hospital was performed for neutrophil CD64 expression, C-reactive protein, erythrocyte sedimentation rate, and complete blood count. The laboratory findings were compared with a clinical score for the likelihood of infection/sepsis, which was obtained by a blinded retrospective chart review. Results.—The diagnostic performance, as gauged by the clinical score, varied with neutrophil CD64 (sensitivity 87.9%, specificity 71.2%, efficiency 76.8%) and outperformed C-reactive protein (sensitivity 88.2%, specificity 59.4%, efficiency 69.4%), absolute neutrophil count (sensitivity 60.0%, specificity 50.8%, efficiency 53.8%), myeloid left shift (sensitivity 68.2%, specificity 76.3%, efficiency 73.3%), and sedimentation rate (sensitivity 50.0%, specificity 65.5%, efficiency 61.0%). Conclusion.—Neutrophil CD64 expression quantitation provides improved diagnostic detection of infection/sepsis compared with the standard diagnostic tests used in current medical practice.

scholarly journals Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Kyung Mi Park ◽  
Da Jung Chung ◽  
Mijin Choi ◽  
Taejoon Kang ◽  
Jinyoung Jeong
Keyword(s):  
Chemical Species ◽  
Test Strip ◽  
Disease Diagnosis ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Lateral Flow Immunochromatographic Assay

Abstract A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.

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