A Genetic Screen to Identify Components of the sina Signaling Pathway in Drosophila Eye Development

Genetics ◽  
1998 ◽  
Vol 148 (1) ◽  
pp. 277-286 ◽  
Author(s):  
Thomas P Neufeld ◽  
Amy H Tang ◽  
Gerald M Rubin
Keyword(s):  
Cell Fate ◽  
Eye Development ◽  
Ectopic Expression ◽  
Genetic Screen ◽  
Cell Fate Specification ◽  
Morphogenetic Furrow ◽  
Drosophila Homolog ◽  
Drosophila Eye ◽  
Ubiquitin Conjugating Enzyme ◽  
Seven In Absentia

Abstract Specification of the R7 photoreceptor cell in the developing Drosophila eye requires the seven in absentia (sina) gene. We demonstrate that ectopic expression of sina in all cells behind the morphogenetic furrow disrupts normal eye development during pupation, resulting in a severely disorganized adult eye. Earlier events of cell fate specification appear unaffected. A genetic screen for dominant enhancers and suppressors of this phenotype identified mutations in a number of genes required for normal eye development, including UbcD1, which encodes a ubiquitin conjugating enzyme; SR3-4a, a gene previously implicated in signaling downstream of Ras1; and a Drosophila homolog of the Sin3A transcriptional repressor.

A Genetic Screen to Identify Components of the sina Signaling Pathway in Drosophila Eye Development

Genetics ◽  
1998 ◽  
Vol 148 (1) ◽  
pp. 277-286
Author(s):  
Thomas P Neufeld ◽  
Amy H Tang ◽  
Gerald M Rubin
Keyword(s):  
Cell Fate ◽  
Eye Development ◽  
Ectopic Expression ◽  
Genetic Screen ◽  
Cell Fate Specification ◽  
Morphogenetic Furrow ◽  
Drosophila Homolog ◽  
Drosophila Eye ◽  
Ubiquitin Conjugating Enzyme ◽  
Seven In Absentia

AbstractSpecification of the R7 photoreceptor cell in the developing Drosophila eye requires the seven in absentia (sina) gene. We demonstrate that ectopic expression of sina in all cells behind the morphogenetic furrow disrupts normal eye development during pupation, resulting in a severely disorganized adult eye. Earlier events of cell fate specification appear unaffected. A genetic screen for dominant enhancers and suppressors of this phenotype identified mutations in a number of genes required for normal eye development, including UbcD1, which encodes a ubiquitin conjugating enzyme; SR3-4a, a gene previously implicated in signaling downstream of Ras1; and a Drosophila homolog of the Sin3A transcriptional repressor.

Ectopic expression of either the Drosophila gooseberry-distal or proximal gene causes alterations of cell fate in the epidermis and central nervous system

Development ◽  
1994 ◽  
Vol 120 (5) ◽  
pp. 1151-1161 ◽  
Author(s):  
Y. Zhang ◽  
A. Ungar ◽  
C. Fresquez ◽  
R. Holmgren
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Fate ◽  
Ectopic Expression ◽  
Cell Fate Specification ◽  
Single Function ◽  
Independent Functions ◽  
Segment Polarity ◽  
The Central Nervous System ◽  
The Common

Previous studies have shown that the segment polarity locus gooseberry, which contains two closely related transcripts gooseberry-proximal and gooseberry-distal, is required for proper development in both the epidermis and the central nervous system of Drosophila. In this study, the roles of the gooseberry proteins in the process of cell fate specification have been examined by generating two fly lines in which either gooseberry-distal or gooseberry-proximal expression is under the control of an hsp70 promoter. We have found that ectopic expression of either gooseberry protein causes cell fate transformations that are reciprocal to those of a gooseberry deletion mutant. Our results suggest that the gooseberry-distal protein is required for the specification of naked cuticle in the epidermis and specific neuroblasts in the central nervous system. These roles may reflect independent functions in neuroblasts and epidermal cells or a single function in the common ectodermal precursor cells. The gooseberry-proximal protein is also found in the same neuroblasts as gooseberry-distal and in the descendants of these cells.

Identifying targets of the rough homeobox gene of Drosophila: evidence that rhomboid functions in eye development

Development ◽  
1992 ◽  
Vol 116 (2) ◽  
pp. 335-346 ◽  
Author(s):  
M. Freeman ◽  
B.E. Kimmel ◽  
G.M. Rubin
Keyword(s):  
Cell Fate ◽  
Target Genes ◽  
Eye Development ◽  
Expression Patterns ◽  
Ectopic Expression ◽  
Homeobox Gene ◽  
Homeodomain Protein ◽  
Dorsoventral Patterning ◽  
Altered Expression ◽  
Enhancer Trap Lines

In order to identify potential target genes of the rough homeodomain protein, which is known to specify some aspects of the R2/R5 photoreceptor subtype in the Drosophila eye, we have carried out a search for enhancer trap lines whose expression is rough-dependent. We crossed 101 enhancer traps that are expressed in the developing eye into a rough mutant background, and have identified seven lines that have altered expression patterns. One of these putative rough target genes is rhomboid, a gene known to be required for dorsoventral patterning and development of some of the nervous system in the embryo. We have examined the role of rhomboid in eye development and find that, while mutant clones have only a subtle phenotype, ectopic expression of the gene causes the non-neuronal mystery cells to be transformed into photoreceptors. We propose that rhomboid is a part of a partially redundant network of genes that specify photoreceptor cell fate.

scholarly journals Musashi and Seven in absentia downregulate Tramtrack through distinct mechanisms in Drosophila eye development

1999 ◽  
Vol 87 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
Yuki Hirota ◽  
Masataka Okabe ◽  
Takao Imai ◽  
Mitsuhiko Kurusu ◽  
Atsuyo Yamamoto ◽  
...  
Keyword(s):  
Eye Development ◽  
Drosophila Eye ◽  
Seven In Absentia

Ectopic Expression of the Immune Adaptor Protein CD3zeta in Neural Stem/Progenitor Cells Disrupts Cell-Fate Specification

2011 ◽  
Vol 46 (2) ◽  
pp. 431-441 ◽  
Author(s):  
Julie Angibaud ◽  
Stéphane J. Baudouin ◽  
Antoine Louveau ◽  
Véronique Nerrière-Daguin ◽  
Virginie Bonnamain ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cell Fate ◽  
Ectopic Expression ◽  
Adaptor Protein ◽  
Cell Fate Specification ◽  
Fate Specification ◽  
Neural Stem Progenitor Cells

Role of the morphogenetic furrow in establishing polarity in the Drosophila eye

Development ◽  
1995 ◽  
Vol 121 (12) ◽  
pp. 4085-4094 ◽  
Author(s):  
F. Chanut ◽  
U. Heberlein
Keyword(s):  
Ectopic Expression ◽  
Morphogenetic Furrow ◽  
Eye Disc ◽  
Drosophila Eye ◽  
Retinal Epithelium ◽  
Anteroposterior Polarity ◽  
Crystalline Array

The Drosophila retina is a crystalline array of 800 ommatidia whose organization and assembly suggest polarization of the retinal epithelium along anteroposterior and dorsoventral axes. The retina develops by a stepwise process following the posterior-to-anterior progression of the morphogenetic furrow across the eye disc. Ectopic expression of hedgehog or local removal of patched function generates ectopic furrows that can progress in any direction across the disc leaving in their wake differentiating fields of ectopic ommatidia. We have studied the effect of these ectopic furrows on the polarity of ommatidial assembly and rotation. We find that the anteroposterior asymmetry of ommatidial assembly parallels the progression of ectopic furrows, regardless of their direction. In addition, ommatidia developing behind ectopic furrows rotate coordinately, forming equators in various regions of the disc. Interestingly, the expression of a marker normally restricted to the equator is induced in ectopic ommatidial fields. Ectopic equators are stable as they persist to adulthood, where they can coexist with the normal equator. Our results suggest that ectopic furrows can impart polarity to the disc epithelium, regarding the direction of both assembly and rotation of ommatidia. We propose that these processes are polarized as a consequence of furrow propagation, while more global determinants of dorsoventral and anteroposterior polarity may act less directly by determining the site of furrow initiation.

Ectopic expression of seven-up causes cell fate changes during ommatidial assembly

Development ◽  
1993 ◽  
Vol 118 (4) ◽  
pp. 1123-1135 ◽  
Author(s):  
Y. Hiromi ◽  
M. Mlodzik ◽  
S.R. West ◽  
G.M. Rubin ◽  
C.S. Goodman
Keyword(s):  
Cell Fate ◽  
Receptor Tyrosine Kinase ◽  
Essential Role ◽  
Ectopic Expression ◽  
Spatiotemporal Pattern ◽  
Morphogenetic Furrow ◽  
Cellular Origin ◽  
Cone Cells ◽  
Sevenless Receptor Tyrosine Kinase ◽  
Photoreceptor Neurons

During Drosophila ommatidial development, a single cell is selected within the ommatidial cluster to become the R7 photoreceptor neuron. The seven-up gene has been shown to play a role in this process by preventing four other photoreceptor precursors, R3/R4/R1/R6, from adopting the R7 cell fate. The seven-up gene encodes a steroid receptor-like molecule that is expressed only in those four cells that require seven-up function in the developing Drosophila ommatidium. We have examined the functional significance of the spatially restricted expression of seven-up by misexpressing seven-up isoforms. As expected from the function that seven-up performs in R3/R4/R1/R6, ubiquitous expression of seven-up causes transformation of the R7 cell to an R1-R6 cell fate. In addition, depending on the timing and spatial pattern of expression, various other phenotypes are produced including the loss of the R7 cell and the formation of extra R7 cells. Ubiquitous expression of seven-up close to the morphogenetic furrow interferes with R8 differentiation resulting in failure to express the boss protein, the ligand for the sevenless receptor tyrosine kinase, and the R7 cell is lost consequently. Extra R7 cells are formed by recruiting non-neuronal cone cells as photoreceptor neurons in a sevenless and bride of sevenless independent way. Thus, the spatiotemporal pattern of seven-up expression plays an essential role in controlling the number and cellular origin of the R7 neuron in the ommatidium. Our results also suggest that seven-up controls decisions not only between photoreceptor subtypes, but also between neuronal and non-neuronal fates.

Glued participates in distinct microtubule-based activities in Drosophila eye development

Development ◽  
1997 ◽  
Vol 124 (8) ◽  
pp. 1497-1507 ◽  
Author(s):  
S.S. Fan ◽  
D.F. Ready
Keyword(s):  
Cell Fate ◽  
Eye Development ◽  
Cytoplasmic Dynein ◽  
Dominant Negative ◽  
Multiple Roles ◽  
Cell Fate Determination ◽  
Stage Of Development ◽  
Multiple Phenotypes ◽  
Drosophila Homolog ◽  
Distinct Features

A C-terminal truncation of Glued, the Drosophila homolog of the cytoplasmic dynein activating protein, dynactin, results in a severe and complex retinal phenotype, including a roughening of the facet array, malformation of the photosensitive rhabdomeres, and a general deficit and disorder of retinal cells. We have characterized the developmental phenotype in Glued1 and found defects in multiple stages of eye development, including mitosis, nuclear migration, cell fate determination, rhabdomere morphogenesis and cell death. Transgenic flies that express dominant negative Glued under heat-shock control reproduce distinct features of the original Glued1 phenotype depending on the stage of development. The multiple phenotypes effected by truncated Glued point to the multiple roles served by dynactin/dynein during eye development.

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