scholarly journals Suppressors of an Arabidopsis thaliana phyB Mutation Identify Genes That Control Light Signaling and Hypocotyl Elongation

Genetics ◽  
1998 ◽  
Vol 148 (3) ◽  
pp. 1295-1310
Author(s):  
Jason W Reed ◽  
Rangasamy P Elumalai ◽  
Joanne Chory
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Elongation ◽  
Hypocotyl Elongation ◽  
Light Signaling ◽  
Ambient Light ◽  
Phytochrome B ◽  
Light Responses ◽  
Genetic Analyses ◽  
Control Light

Abstract Ambient light controls the development and physiology of plants. The Arabidopsis thaliana photoreceptor phytochrome B (PHYB) regulates developmental light responses at both seedling and adult stages. To identify genes that mediate control of development by light, we screened for suppressors of the long hypocotyl phenotype caused by a phyB mutation. Genetic analyses show that the shy (short hypocotyl) mutations we have isolated fall in several loci. Phenotypes of the mutants suggest that some of the genes identified have functions in control of light responses. Other loci specifically affect cell elongation or expansion.

Aquaporin AtTIP5;1 as an essential target of gibberellins promotes hypocotyl cell elongation in Arabidopsis thaliana under excess boron stress

2018 ◽  
Vol 45 (3) ◽  
pp. 305 ◽  
Author(s):  
Yongqi Pang ◽  
Jintong Li ◽  
Bishu Qi ◽  
Mi Tian ◽  
Lirong Sun ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Elongation ◽  
Stem Elongation ◽  
Hypocotyl Elongation ◽  
Wild Type ◽  
Downstream Target ◽  
Della Proteins ◽  
High Concentrations ◽  
Tonoplast Aquaporin ◽  
Ga Biosynthesis

Aquaporins play essential roles in growth and development including stem elongation in plants. Tonoplast aquaporin AtTIP5;1 has been proposed to positively regulate hypocotyl elongation under high concentrations of boron (high-B) in Arabidopsis thaliana (L.) Heynh. However, the mechanism underlying this process remains unanswered. Here, we show that paclobatrazol, an inhibitor of GA biosynthesis, significantly suppressed the hypocotyl cell elongation of wild-type (WT) seedlings, and more strongly suppressed that of AtTIP5;1 overexpressors under high-B stress. Two AtTIP5;1 null mutants displayed arrested elongation of cells in the upper part of hypocotyls compared with the WT in the presence of high-B or GA3. Moreover, paclobatrazol treatment completely inhibited the increases in AtTIP5;1 transcripts induced by high-B, whereas GA3 application upregulated AtTIP5;1 expression in the WT. In addition, treatment with high-B remarkably elevated the expression levels of GA3ox1, GA20ox1 and GA20ox2 – key biosynthesis genes of GAs – in WT seedlings. The GA3 and GA4 content also increased in WT seedlings grown in MS medium containing high-B. Additionally, application of high-B failed to enhance AtTIP5;1 expression in the double mutant rga-24–gai-t6 of DELLA genes. Together, these results suggest that AtTIP5;1 is an essential downstream target of GAs. High-B induces the accumulation of GAs, which activates AtTIP5;1 through modulation of the DELLA proteins Repressor of ga1–3 and GA-insensitive, further promoting hypocotyl elongation in A. thaliana.

scholarly journals PHYTOCHROME B and HISTONE DEACETYLASE 6 Control Light-Induced Chromatin Compaction in Arabidopsis thaliana

PLoS Genetics ◽  
2009 ◽  
Vol 5 (9) ◽  
pp. e1000638 ◽  
Author(s):  
Federico Tessadori ◽  
Martijn van Zanten ◽  
Penka Pavlova ◽  
Rachel Clifton ◽  
Frédéric Pontvianne ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Histone Deacetylase ◽  
Phytochrome B ◽  
Histone Deacetylase 6 ◽  
Chromatin Compaction ◽  
Control Light

Stem-piped light activates phytochrome B to trigger light responses in Arabidopsis thaliana roots

2016 ◽  
Vol 9 (452) ◽  
pp. ra106-ra106 ◽  
Author(s):  
H.-J. Lee ◽  
J.-H. Ha ◽  
S.-G. Kim ◽  
H.-K. Choi ◽  
Z. H. Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Phytochrome B ◽  
Light Responses

scholarly journals Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

2005 ◽  
Vol 46 (5) ◽  
pp. 790-796 ◽  
Author(s):  
Aurora Piñas Fernández ◽  
Patricia Gil ◽  
Ildiko Valkai ◽  
Ferenc Nagy ◽  
Eberhard Schäfer
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Plumbaginifolia ◽  
Phytochrome B

scholarly journals Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B

2021 ◽  
Author(s):  
Chan Yul Yoo ◽  
Qing Sang ◽  
Jiangman He ◽  
Yongjian Qiu ◽  
Lingyun Long ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Target Genes ◽  
Transcription Activation ◽  
Phytochrome B ◽  
Activation Domain ◽  
Light Responses ◽  
Dark Light ◽  
Transactivation Activity ◽  
Transcription Activation Domain

Phytochrome B (PHYB) triggers diverse light responses in Arabidopsis by binding to a group of antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) to promote PIF degradation, consequently downregulating PIF target genes. However, whether PHYB directly controls the transactivation activity of PIFs remains ambiguous. Here we show that the prototypic PIF, PIF3, possesses a p53-like transcription activation domain (TAD) consisting of a sequence-specific, hydrophobic activator motif surrounded by acidic residues. A PIF3mTAD mutant in which the activator motif is replaced with alanines fails to activate PIF3 target genes in Arabidopsis in dark, light, and shade conditions, validating the in vivo functions of the PIF3 TAD. Intriguingly, binding of the N-terminal photosensory module of PHYB to the PHYB-binding site adjacent to the TAD inhibits its transactivation activity. These results unveil a photoresponsive transcriptional switching mechanism in which photoactivated PHYB directly masks the transactivation activity of PIF3. Our study also suggests the unexpected conservation of sequence-specific TADs between the animal and plant kingdoms.

scholarly journals Arabidopsis thaliana RALF1 opposes brassinosteroid effects on root cell elongation and lateral root formation

2014 ◽  
Vol 65 (8) ◽  
pp. 2219-2230 ◽  
Author(s):  
Tábata Bergonci ◽  
Bianca Ribeiro ◽  
Paulo H.O. Ceciliato ◽  
Juan Carlos Guerrero-Abad ◽  
Marcio C. Silva-Filho ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Elongation ◽  
Lateral Root ◽  
Root Formation ◽  
Root Cell ◽  
Lateral Root Formation

Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh

Development ◽  
1996 ◽  
Vol 122 (5) ◽  
pp. 1589-1600 ◽  
Author(s):  
T. Tsuge ◽  
H. Tsukaya ◽  
H. Uchimiya
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Elongation ◽  
Leaf Development ◽  
Cellular Level ◽  
Leaf Length ◽  
Specific Cell ◽  
Wild Type ◽  
Leaf Morphogenesis ◽  
Length Direction ◽  
Number Of Cells

For genetic analysis of mechanisms of leaf morphogenesis, we chose Arabidopsis thaliana (L.) Heynh. as a model for leaf development in dicotyledonous plants. Leaves of the angustifolia mutant were the same length as but narrower and thicker than wild-type leaves. The total number of cells in leaf blades of angustifolia plants was the same as in the wild type. At the cellular level in the angustifolia mutant it was found that the cells were smaller in the leaf-width direction and larger in the leaf-thickness direction than in wild type, revealing the function of the ANGUSTIFOLIA gene, which is to control leaf morphology by regulating polarity-specific cell elongation. The existence of similar genes that regulate leaf development in the length direction was, therefore, predicted. Three loci and several alleles associated with short-leaved mutants were newly isolated as rotundifolia mutants. The rotundifolia3 mutant had the same number of cells as the wild type, with reduced cell elongation in the leaf-length direction. The features of the angustifolia rotundifolia3 double mutant indicated that ANGUSTIFOLIA and ROTUNDIFOLIA3 genes act independently. We propose that leaf expansion in Arabidopsis involves at least two independent developmental processes: width development and length development, with the ANGUSTIFOLIA and ROTUNDIFOLIA3 genes playing different polarity-specific roles in cell elongation.

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