scholarly journals DELAYED RECOVERY FROM CARBON DIOXIDE IN DROSOPHILA MELANOGASTER

Genetics ◽  
1964 ◽  
Vol 50 (4) ◽  
pp. 509-526
Author(s):  
William B McCrady ◽  
Ralph L Sulerud
Keyword(s):  
Carbon Dioxide ◽  
Drosophila Melanogaster ◽  
Delayed Recovery

scholarly journals INDEPENDENT PRODUCTION OF CO2 SENSITIVITY BY NUCLEAR GENE DLY AND A TRANSMISSIBLE AGENT FROM DELAYED-RECOVERY DROSOPHILA MELANOGASTER

Genetics ◽  
1979 ◽  
Vol 92 (2) ◽  
pp. 503-510
Author(s):  
M A Clark ◽  
W B McCrady ◽  
C L Fielding
Keyword(s):  
Carbon Dioxide ◽  
Drosophila Melanogaster ◽  
Nuclear Gene ◽  
Infectious Agent ◽  
Mutant Gene ◽  
Co2 Sensitivity ◽  
The Third ◽  
Male Line ◽  
Delayed Recovery ◽  
Sigma Virus

ABSTRACT Flies of stocks designated delayed-recovery by MCCRADY and SULERUD (1964) remain temporarily paralyzed after exposure to carbon dioxide. This condition is similar to CO2 sensitivity, which occurs in flies infected with the maternally transmitted sigma virus, but is due, at least in part, to the third chromosome mutant gene DlY. Because earlier work indicated that extracts of delayed-recovery flies could occasionally transmit CO2, sensitivity when injected into resistant recipients, we have tested the possibility that some delayed-recovery stocks contain a sigma-like transmissible virus, in addition to the Dly gene. We found that TDR-orange, a stock derived from the original delayed-recovery line, and temperature-cured populations of the same stock, both contain some agent that is transmissible by injection. TDR-BC3f, a stock derived by backcrossing through the male line to eliminate maternally transmitted factors, does not appear to contain such an infectious agent, but remains sensitive to CO2. These observations lead us to the conclusion that the originally described delayed-recovery stocks harbor an infectious extrachromosomal agent, in addition to possessing the Dly gene, and each is capable of producing a sensitivity to carbon dioxide.

Effects of pollutants from power plants in Kosova on genetic loads of Drosophila melanogaster

2014 ◽  
Vol 32 (7) ◽  
pp. 1310-1317 ◽  
Author(s):  
Avdulla J Alija ◽  
Ismet D Bajraktari ◽  
Hidajete Muharremi ◽  
Nikolaus Bresgen ◽  
Peter M Eckl
Keyword(s):  
Heavy Metals ◽  
Carbon Dioxide ◽  
Drosophila Melanogaster ◽  
Organic Compounds ◽  
Nutrient Medium ◽  
Power Plants ◽  
Genetic Load ◽  
The Other ◽  
European Standard ◽  
Recessive Mutations

It has been reported by the Ministry of Environment in Kosova that particle emissions from one of the units of the coal-fired power plants (Kosova A) in Kastriot/Obiliq were exceeding the European standard by some 74 times. Besides the particle emission, there is also release of sulphur dioxide, mono-nitrogen oxide (NOx), carbon monoxide, carbon dioxide, organic compounds and heavy metals. In addition, there is also release of heavy metals and organic compounds from a nearby solid waste dumpsite. Together, they are considered to be responsible for the increased health problems of the population living in the vicinity.To study the genetic effects of these emissions we focused on the genetic load, that is, recessive mutations that affect the fitness of their carriers, of exposed wild living Drosophila melanogaster. The effects of ash from the dumpsite on the other hand were investigated upon feeding the ash with the nutrient medium. Our results revealed that the D. melanogaster population from the Kastriot/Obiliq area carries a high genetic load of 54.7%. Drosophila fed with the nutrient medium containing ash in a concentration of 1% carried a genetic load of 37.1%, whilst increasing concentrations (2% and 3% of ash) led to higher genetic loads of 68.7% and 67.4%, respectively.

Carbon dioxide triggers recovery from dauer juvenile stage in entomopathogenic nematodes (Heterorhabditis spp.)

Nematology ◽  
2000 ◽  
Vol 2 (3) ◽  
pp. 319-324 ◽  
Author(s):  
Peter Jessen ◽  
Reiner Luttmann ◽  
Ralf-Udo Ehlers ◽  
Olaf Strauch ◽  
Urs Wyss
Keyword(s):  
Carbon Dioxide ◽  
Liquid Culture ◽  
Spontaneous Recovery ◽  
Photorhabdus Luminescens ◽  
Variable Threshold ◽  
Threshold Response ◽  
Third Stage ◽  
Delayed Recovery ◽  
Food Signal ◽  
Bioreactor Process

AbstractHeterorhabditis spp. (Rhabditida: Nematoda) live in a close symbiosis with the bacterium Photorhabdus luminescens. For biocontrol purposes the nematodes are produced in liquid culture pre-incubated with P. luminescens. The bacteria produce a food signal, inducing dauer juveniles (DJ) to initiate development. In rhabditid nematodes the exit from this developmentally arrested third stage DJ is called recovery. Attempts to produce Heterorhabditis spp. in liquid culture have often failed due to low and delayed recovery of the inoculated DJ. The influence of carbon dioxide as a recovery co-factor was investigated. Increasing concentrations of CO2 enhanced DJ recovery in the presence of the bacterial food signal. The effect could not be related to a decline of the pH caused by increasing CO2 concentrations. On the contrary, at lower pH the DJ recovery decreased. In one experiment a considerable spontaneous recovery was observed in the absence of a food signal. This phenomenon and a variable threshold response of the DJ to CO2 lead to the assumption that they are differently pre-disposed to respond to recovery inducing signals. Providing the results can be confirmed in laboratory scale bioreactors, the control of carbon dioxide during nematode liquid culture can help to improve the bioreactor process technology.Heterorhabditis spp. (Rhabditida: Nematoda) leben in enger Symbiose mit dem Bakterium Photorhabdus luminescens. Für die biologische Bekämpfung werden die Nematoden in Flüssigkulturen vermehrt, die vorher mit P. luminescens inkubiert wurden. Die Bakterien produzieren ein Nahrungssignal, das die Dauerlarven (DJ) veranlasst, ihre Entwicklung wieder aufzunehmen. Bei rhabditiden Nematoden wird das Verlassen des entwicklungsphysiologisch gehemmten Dauerlarvenstadiums als “recovery” bezeichnet. Versuche, Heterorhabditis spp. in Flüssigkultur zu produzieren sind oft aufgrund einer niedrigen oder verspäteten “recovery” gescheitert. Der Einfluß von Kohlendioxid als Einflussfaktor auf die “recovery” wurde untersucht. Zunehmende CO2 Konzentrationen förderten die “recovery” bei Anwesenheit des Nahrungssignals. Einem mit zunehmender CO2-Konzentration fallenden pH-Wert konnte die Wirkung nicht zugeschrieben werden. Im Gegenteil, bei niedrigen pH-Werten nahm die “recovery” ab. In einem Experiment wurde eine spontane “recovery” beobachtet, ohne dass ein Nahrungssignal vorhanden war. Dieses Phänomen und die variable Antwort der Dauerlarven auf gleiche CO2-Konzentrationen lassen den Schluss zu, dass die Dauerlarven unterschiedlich prädisponiert sind in ihrer Reaktion auf die “recovery” induzierenden Signale. Vorausgesetzt die Ergebnisse können in LaborBioreaktoren bestätigt werden, ist die Regelung des Kohlendioxidgehalts während der Nematoden-Flüssigkultur eine Hilfe die Prozesstechnik zu optimieren.

Effects of carbon dioxide anaesthesia on Drosophila melanogaster

1972 ◽  
Vol 18 (10) ◽  
pp. 1869-1874 ◽  
Author(s):  
J.M. Perron ◽  
L. Huot ◽  
G.-W. Corrivault ◽  
S.S. Chawla
Keyword(s):  
Carbon Dioxide ◽  
Drosophila Melanogaster

scholarly journals Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anaesthesia

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Nathan R. Bartholomew ◽  
Jacob M. Burdett ◽  
John M. VandenBrooks ◽  
Michael C. Quinlan ◽  
Gerald B. Call
Keyword(s):  
Carbon Dioxide ◽  
Drosophila Melanogaster ◽  
Flight Behaviour

scholarly journals A NEW STRAIN OF DROSOPHILA THAT INHIBITS THE DEVELOPMENT OF SYMPTOMS IN IMAGOES INFECTED WITH SIGMA VIRUS

Genetics ◽  
1978 ◽  
Vol 88 (3) ◽  
pp. 505-513
Author(s):  
R S Herforth
Keyword(s):  
Carbon Dioxide ◽  
Drosophila Melanogaster ◽  
Incubation Time ◽  
Infectious Virus ◽  
Mutant Gene ◽  
Autosomal Gene ◽  
Wild Type ◽  
Sigma Virus ◽  
Relationship Of ◽  
The Relationship

ABSTRACT A strain of Drosophila melanogaster bearing the mutant gene ebony has been found to slow the development of symptoms (carbon dioxide sensitivity) in adult flies inoculated with sigma virus, a member of the rhabdovirus group. This inhibition is made evident by comparing mean incubation times of the virus in ebony and wild-type (Oregon) flies. The increase in mean incubation time in ebony flies has ranged from about 3 to 8 days, depending on the virus strain, amount of virus injected, and the age of the flies at the time of inoculation. This delay in development of symptoms appears to be due to a dominant autosomal gene, although further work is needed to confirm this. When accumulation of infectious virus after inoculation is compared in ebony and Oregon flies, there seems to be no inhibition of multiplication in ebony at the level of the entire fly. The relationship of this work to current theories on the mechanism of symptom production by sigma virus is discussed.

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