scholarly journals Acinetobacter baumanniiK27 and K44 capsular polysaccharides have the same K unit but different structures due to the presence of distinctwzygenes in otherwise closely related K gene clusters

Glycobiology ◽  
2015 ◽  
Vol 26 (5) ◽  
pp. 501-508 ◽  
Author(s):  
Alexander S Shashkov ◽  
Johanna J Kenyon ◽  
Sof'ya N Senchenkova ◽  
Mikhail M Shneider ◽  
Anastasiya V Popova ◽  
...  
Keyword(s):  
Gene Clusters ◽  
Capsular Polysaccharides

scholarly journals Genetic Study of Capsular Switching between Neisseria meningitidis Sequence Type 7 Serogroup A and C Strains

2010 ◽  
Vol 78 (9) ◽  
pp. 3883-3888 ◽  
Author(s):  
Quan Wang ◽  
Zhujun Shao ◽  
Xiaoting Wang ◽  
Yuan Gao ◽  
Machao Li ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Neisseria Meningitidis ◽  
Genetic Study ◽  
Gel Electrophoresis ◽  
Genetic Basis ◽  
Gene Clusters ◽  
Unique Sequence ◽  
Pulsed Field Gel Electrophoresis ◽  
Sequence Type ◽  
Capsular Polysaccharides

ABSTRACT Neisseria meningitidis is a leading cause of septicemia and meningitis worldwide. N. meningitidis capsular polysaccharides have been classified into 13 distinct serogroups which are defined by antibody reactivity and structural analysis, and the capsule plays an important role in virulence. Serogroups A, B, C, W135, and Y have been reported to be clinically important. Several newly identified serogroup C isolates belonging to the unique sequence type 7 (ST-7) were identified in China. Since most ST-7 isolates from China belonged to serogroup A, the newly identified ST-7 serogroup C strains were proposed to have arisen from those belonging to ST-7 serogroup A. In this study, six ST-7 serogroup C and three ST-7 serogroup A isolates were analyzed by pulsed-field gel electrophoresis to confirm their sequence type. In order to clarify the genetic basis of capsular switching between ST-7 serogroup A and C strains, the whole capsular gene clusters and surrounding genes of the two representative ST-7 strains belonging to serogroups A and C, respectively, were sequenced and compared. Potential recombination sites were analyzed using the RDP3 beta software, and recombination-related regions in two other ST-7 serogroup A and five ST-7 serogroup C strains were also sequenced and compared to the representative ST-7 serogroup A and C strain sequences.

Related structures of neutral capsular polysaccharides of Acinetobacter baumannii isolates that carry related capsule gene clusters KL43, KL47, and KL88

2016 ◽  
Vol 435 ◽  
pp. 173-179 ◽  
Author(s):  
Alexander S. Shashkov ◽  
Johanna J. Kenyon ◽  
Nikolay P. Arbatsky ◽  
Mikhail M. Shneider ◽  
Anastasiya V. Popova ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Gene Clusters ◽  
Capsular Polysaccharides

scholarly journals Common organization of gene clusters for production of different capsular polysaccharides (K antigens) in Escherichia coli.

1988 ◽  
Vol 170 (3) ◽  
pp. 1305-1310 ◽  
Author(s):  
I S Roberts ◽  
R Mountford ◽  
R Hodge ◽  
K B Jann ◽  
G J Boulnois
Keyword(s):  
Escherichia Coli ◽  
Gene Clusters ◽  
Capsular Polysaccharides

scholarly journals Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides

2021 ◽  
Vol 22 (11) ◽  
pp. 5641
Author(s):  
Anastasiya A. Kasimova ◽  
Nikolay P. Arbatsky ◽  
Jacob Tickner ◽  
Johanna J. Kenyon ◽  
Ruth M. Hall ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Capsular Polysaccharide ◽  
Gene Clusters ◽  
13C Nmr Spectroscopy ◽  
Side Chain ◽  
Capsular Polysaccharides ◽  
Two Dimensional ◽  
Genome Sequences ◽  
1H And 13C Nmr ◽  
Biosynthesis Gene

Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.

The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique.

2020 ◽  
Vol 3 (11) ◽  
pp. 1100-1104
Author(s):  
Hussein Naeem Aldhaheri ◽  
Ihsan Edan AlSaimary ◽  
Murtadha Mohammed ALMusafer
Keyword(s):  
Personal Information ◽  
Receptor Gene ◽  
Specific Antigen ◽  
Gene Clusters ◽  
Control Group ◽  
P Value ◽  
Group Data ◽  
Elisa Technique ◽  
And Control

      The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

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