The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique.

2020 ◽  
Vol 3 (11) ◽  
pp. 1100-1104
Author(s):  
Hussein Naeem Aldhaheri ◽  
Ihsan Edan AlSaimary ◽  
Murtadha Mohammed ALMusafer

      The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.

2020 ◽  
Vol 3 (11) ◽  
pp. 1105-1109
Author(s):  
Ihsan Edan AlSaimary ◽  
Hussein Naeem Aldhaheri ◽  
Murtadha Mohammed ALMusafer

The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected , The molecular expression study include extracting DNA from blood of Prostatitis patients , Prostitis patients and Control group by using specific primers for conventional PCR and Real Time PCR , the amplification of all extracted DNA from blood samples was preform and confirm by using electrophoresis with (100volt/30min).   PCR product was 149bp for TLR7 on agarose gel (1%), (50voltage for 1hour) with a presence 100%. The results of the present study indicate that the Toll like receptor alleles associated with risk of prostatitis.


2020 ◽  
pp. 1-4
Author(s):  
Ihsan E AlSaimary ◽  
◽  
Hussein N AlDhaheri ◽  
Murtadha M ALMusafer ◽  
◽  
...  

Determine Immunogenetic expression of Toll-like receptor gene clusters related to prostatitis was carried in this investigation to give acknowledge about Role of TLR in prostatitis immunity in men. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected , The molecular expression study include extracting DNA from blood of Prostatitis patients, Prostitis patients and Control group by using specific primers for conventional PCR and Real Time PCR , the amplification of all extracted DNA from blood samples was preform and confirm by using electrophoresis with (100volt/30min). The estimation revealed that the amplified DNA (PCR product) was 135bp for TLR1 (at 50 voltage for 1hour) with the presence of TLR1 was 75% of total samples, and for TLR2 the PCR product was 125bp on agarose gel (1%), (50volt/1hour) with a presence of 95% from total samples. The results of the present study indicate that the Toll like receptor alleles associated with risk of prostatitis.


2020 ◽  
Vol 9 ◽  
pp. 1918 ◽  
Author(s):  
Mahnaz Sadeghi-Shabestari ◽  
Yalda Jabbari Moghaddam ◽  
Hasan Rezapoor ◽  
Mojataba Sohrabpour

Background: Allergic rhinitis (AR) is one of the most common diseases in the world and affects about 10-50% of the general population. Probiotics are live microorganisms that help the normal state of the intestine, and if prescribed correctly, they can stimulate the mucosal immune system to prevent inflammatory symptoms of allergy and atopy. The present study aims to investigate the role of probiotics in the treatment of AR when added to standard therapy as adjuvant agents. Materials and Methods: In this clinical trial study, 28 patients older than 15 years with AR randomly divided into probiotics and control groups. The probiotics group received standard therapy for AR accompanied by probiotic capsules every 12 hours for eight weeks, whereas the control group received standard therapy for AR with placebo capsules as the same protocol. Data were analyzed using SPSS Version 23 (IBM Corporation, Armonk, NY, USA) and, the P-value less than 0.05 was considered statistically significant. Results: In the probiotics group, 14.3% of patients had sneezing at the baseline, which significantly decreased to 4.6% (P<0.01). Also, the necessity for nasal and oral corticosteroids after treatment with probiotics in the probiotics group was less than the control group (P<0.01). Although cough, nasal discharge, conchae hypertrophy, and night sleep disorders reduced after treatment with probiotics, this reduction was not statistically significant between the two groups. Conclusion: Based on the results of this clinical trial, the use of probiotics had no significant effect on the outcome of patients with AR. [GMJ.2020;9:e1918] 


2017 ◽  
Vol 3 (5) ◽  
pp. 525-532
Author(s):  
Diah Evawanna Anuhgera ◽  
Tjahjono Kuncoro ◽  
Sri Sumarni ◽  
Mardiyono Mardiyono ◽  
Ari Suwondo

Background: Post-cesarean mothers often experience anxiety and discomfort due to long-term pain. The combination of hypnobreastfeeding and acupressure is considered to be effective in reducing anxiety and pain levels. Objective: This study aims to examine the effect of combination of hypnobreastfeeding and acupressure on anxiety and pain levels in post-caesarean mothers.Methods: This study was a true experiment with pretest-posttest control group design, conducted in the Ambarawa Public Hospital on 5 November to 9 December 2016. There were 36 participants selected using stratified random sampling, with 18 assigned in the experiment and control group. Data were analyzed using paired t-test and wilcoxon test.Results: There were statistically significant differences of anxiety and pain levels before and after intervention in the experiment and control group with p-value 0.001 (<0.05).Conclusion: The combination of hypnobreastfeeding and acupressure has a significant effect in reducing anxiety and pain levels in post-cesarean mothers. This intervention could be applied as an alternative therapy in treating post-caesarean mothers.


Author(s):  
Kislay Kumar ◽  
Devesh Kumar ◽  
Pooja T. Pandey ◽  
Divya .

Background: tress at any stage of life virtually produces several respiratory limitations and reduces tolerance to physical efforts, sometimes leading to pulmonary diseases in individuals. Various yoga and Pranayama like Kapalbhati and Nadi-sodhan involves powerful strokes of exhalation, which trains the subject to make full use of diaphragm and abdominal muscles.Methods: This study is performed on 1st yr. medical students whose stress level is in higher side due to academic burden. 55 medical students were selected as participants through counseling and were divided into Yoga group (n= 27) and control group (n=28). Pulmonary functions of subjects were tested using Pony FX advanced desktop spirometer manufactured by Cosmed. Yoga is performed 1 hr/day for 6 days /week for 12 weeks by yoga group.Results: Pulmonary functions tested in Yoga and control group were FVC, FEV1, PEF and FVC/FEV1. There were significant improvements in pulmonary functions in yoga group. In Yoga group (FVC- 5.8% increases, FEV1- 5.2% increase, PEF- 34% increase and FVC/FEV1- 3.15% increase). In control group also, all parameters increased but P value was not significant.Conclusions: This study concludes that practicing Yoga has shown a significant improvement on pulmonary functions in 1st year medical students.


2020 ◽  
Vol 16 ◽  
Author(s):  
Amal A. Mohamed ◽  
Mokhtar M. El-Zawahry ◽  
Omnia I. Tantawi ◽  
Amyan Aalkhalegy ◽  
Lamiaa Abdelfattah Fathalla ◽  
...  

Background:: In the early stages of HCC, it is unsatisfactory to depend on alpha-fetoprotein for diagnosis. Objective:: The current study evaluated the possibility of the two miRNAs which are miRNA-96 and miRNA-224 to act as biomarkers for HCC diagnosis. Methods:: This study included 50 patients with HCV-induced HCC and 50 patients with HCV-induced liver cirrhosis for comparison as well as 67 healthy volunteers as controls. All participants were subjected to history taking, clinical examination, and laboratory investigations as well as quantification of serum miRNA-96 and miRNA-224 by real-time quantitative PCR. Results:: MicroRNA 224 level was significantly higher in HCC than the other two groups and was significantly higher in liver cirrhosis than the control group. MicroRNA 96 level was higher in HCC than the control group and was higher in cirrhotic group than both HCC and control groups. However, it doesn’t reach the statistical significance level. The best cut-off value of microRNA 96 for detecting HCC was 3.414 with a sensitivity of 67% and a specificity of 67%, (p-value <0.001). The best cut-off value of microRNA 224 for detecting HCC was 16.75 with a sensitivity of 88% and a specificity of 85% (p-value<0.001). Conclusion:: miRNA-224 could serve as a biomarker for the HCC diagnosis.


2017 ◽  
Vol 3 (5) ◽  
pp. 496-507
Author(s):  
Santoso Santoso ◽  
Rita Kartika Sari ◽  
Anggorowati Anggorowati

Background: Job satisfaction influences the quality of health services in hospitals. However, low job satisfaction of nurses exists in the literature and influenced by various factors, including supervision. Thus, a reflective supervision model is considered effective in improving nurse job satisfaction.Objective: This study aims to analyze the effect of the reflective supervision model on nurse job satisfaction.Methods: This was a quasi-experimental study with control group design conducted between May-July 2017 in the Tugurejo Hospital and Dr. Moewardi hospital. There were sixty-eight nurses selected using purposive sampling, with 34 nurses assigned in the experiment and control group. Data were collected using questionnaires and then analyzed by paired t-test.Results: Paired t-test obtained p-value of 0.000, which indicated that the reflective supervision model has a significant influence to nurse work satisfaction.Conclusion: The reflective supervision model has a significant effect in increasing nurse job satisfaction. Therefore, this model could be applied by the head nurses to improve nurse outcome indicator, especially for nurse job satisfaction.


2013 ◽  
Vol 7 (10) ◽  
pp. 453
Author(s):  
Nurul Aeni

Angka Kematian Ibu (AKI) di Kabupaten Pati bergerak fluktuatif dan menunjukkan status tertinggi pada tahun 2011 dengan 126 per 100.000 kelahiran hidup. Tujuan penelitian ini adalah menggambarkan kematian ibu di Kabupaten Pati dan menganalisis faktor risiko kematian maternal di Kabupaten Pati Tahun 2011. Penelitian menggunakan pendekatan observasional analitik dengan metode kasus kontrol. Jumlah sampel adalah 24 untuk setiap kelompok kasus dan kelompok kontrol. Analisis data menggunakan uji univariat, bivariat dan multivariat. Hasil dari penelitian adalah tiga penyebab utama kematian ibu di Kabupaten Pati adalah penyakit jantung, preeklamsi/eklamsi, dan perdarahan. Kematian ibu tersebar di 16 kecamatan dari 21 kecamatan yang ada dan sebagian besar kematian terjadi pada masa nifas. Analisis regresi logistik menunjukkan bahwa faktor yang berpengaruh terhadap kematian ibu adalah komplikasi kehamilan (OR = 12,198, nilai p = 0,010), komplikasi persalinan (OR = 9,94, nilai p = 0,020) dan riwayat penyakit (OR = 27,735, nilai p = 0,011). Secara bersama-sama, ketiga variabel tersebut berkontribusi terhadap 64,3% kematian ibu yang terjadi di Kabupaten Pati Tahun 2011.Maternal Mortality Ratio (MMR) of Pati Regency fluctuated and showed the highest state in 2011 with 126 per 100.000 live births. This research aimed to describe maternal mortality cases in Pati Regency and to analyze risk factors of maternal mortality happened in 2011. This research used observational analysis, i.e. cases control study. The number of samples was 24 in each of case group and control group. Data was analyzed with univariate, bivariate, and multivariate. This research resulted that three major causes of maternal mortality were heart disease, preeclampsia/eclampsia, and hemorrhage. Maternal mortality spread on 16 from 21 sub-districts on Pati Regency and the majority of them happened in post delivery period. Logistic regression analysis concluded that risk factors influenced maternal mortality were pregnancy complication (OR = 12.198, p value = 0.010), delivery complication (OR = 9.94, p value = 0.020) and history of illness (OR = 27.735, p value = 0.011). Collectively, those variables contributed to 64.3% of maternal mortality in Pati Regency 2011.


2020 ◽  
Vol 12 (1) ◽  
pp. 51-64
Author(s):  
Nur Rahmat Laba ◽  
Erika Setyanti Kusumaputri

This study aims to determine the effectiveness of gratitude exercise using three good things techniques on happiness at work enhancement. Participants of this study were 15 employees aged 21-28 years divided into two groups: eight participants in the experimental group and seven participants in the control group. The design was a randomized two-group pretest-posttest. Collecting data used to happiness at work scale with Likert model prepared by the researcher. Data analysis methods used by using Mann Whitney U technique for differences of the gain score in the experimental group and control group data score. The result of gain score analysis showed t value = 8.500 and p-value of 0.021 (p<0.05), indicating there is a significant difference between the experimental group and control group after gratitude exercise. The result shows that gratitude exercise using three good things techniques is effective to increase happiness at work of employees.


2020 ◽  
Vol 2 (6) ◽  
pp. 1-2
Author(s):  
Ihsan E AlSaimary ◽  
◽  
Hussein N AlDhaheri ◽  
Murtadha M ALMusafer ◽  
◽  
...  

The Aim of this study was to determine Immunogenetic expression of Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. From total number of (135) patients with prostatitis were taken from two provinces (Basrah and Missan) from the Basrah teaching hospital and Missan teaching hospital that included in the present study , In the clinical study found that patients presented with irritative symptoms with P-value <0.0001. The study also found that patients presented with Obstructive symptoms with P-value <0.0001. The study also found patients presented with Other symptoms with P-value <0.0001. And present study found that patients with Suprapubic Tenderness with P-value <0.0001. And in clinical symptoms show that the age group 40-49 years with P-value <0.0001. Age group 50-59 years found with P-value <0.0001. The third age group 60-69 years and the final age group >70 years with P-value <0.0001.


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