Postnatal Acquisition of Primary Rhesus Cytomegalovirus Infection is Associated With Prolonged Virus Shedding and Impaired CD4+ T Lymphocyte Function

Increased lycopene intake might have cardiovascular benefits, potentially through anti-inflammatory mechanisms. We recently showed that lycopene can influence lymphocyte activity by modulating processes involved in early cellular activation. T lymphocytes comprise different subsets, T cytotoxic, T helper 1 (Th1), T helper 2 (Th2) and T regulatory cells (Treg). We aimed to determine whether lycopene could specifically modulate T-cell subsets function and activity. Peripheral blood mononuclear cells from 11 healthy adults were cultured for 18hr to 60h in the presence of lycopene-enriched liposomes (0-1.18μg lycopene/ml) with or without mitogens. The secretion of cytokines representative of Th1,Th2 and Treg activities were measured by ELISA (IL-2, IL-1β, IL-10, IFN-γ and TGF-β) or cytometric bead array (IL-4, IL-10, IL17 and IFN-γ). The population profile of Tc (CD3+/CD8+), Th (CD3+/CD4+), Treg (CD4+/CD25+), and the Treg subsets nTreg (CD4+/CD25+/FoxP3+) and iTreg (CD4+/CD25+/IL-10+) was determined by flow cytometry. After 18h incubation, IL-2 concentration in the medium was significantly reduced (-29%, p=0.001) in the presence of lycopene (1.18μg/mL). Similar effects were observed after 36h and 60h culture for IFN-γ (-23%, p=0.015), Il-10 (-30%, p=0.023), IL-17 (-30%, p=0.019) but not IL-4 or TGF-β. The proportion of Treg cell was also significantly increased by 36% (p=0.001) in the presence of lycopene (1.18μg/mL) compared with non-treated activated cells. Furthermore, the proportions of iTreg cells were significantly increased by after incubation with lycopene while the proportion of nTreg cells decreased (-20.5 %, p=0.049). We conclude that increased lycopene intake may be beneficial against atherogenesis by modulating T lymphocyte function, particularly in relation toTh1 and Treg.

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Suppressor T lymphocyte function in Graves' disease

Acta Endocrinologica ◽

10.1530/acta.0.1010354 ◽

1982 ◽

Vol 101 (3) ◽

pp. 354-358 ◽

Cited By ~ 8

Author(s):

Bengt Hallengren ◽

Arne Forsgren

Keyword(s):

Patient Group ◽

T Lymphocyte ◽

Pokeweed Mitogen ◽

Control Group ◽

Graves Disease ◽

Lymphocyte Function ◽

Euthyroid State ◽

Hyperthyroid State ◽

Significant Difference ◽

Suppressor T Lymphocyte

Abstract. To explore suppressor T lymphocyte function in Graves' disease, studies were performed in one group of patients in the hyperthyroid state and in another group in the euthyroid state after treatment. Peripheral blood lymphocytes were cultured for 1–7 days., Pokeweed mitogen (PWM; 1.25 μg/ml) was added at the initiation of the cultures or after 24 h. The degree of lymphocyte activation was assessed by measurements of the cellular uptake of [3H]thymidine and expressed in counts per minute (cpm). The suppressor lymphocyte function was estimated by a quotient between the maximum cpm values from cultures with and without pre-incubation. For the hyperthyroid group (n = 15) the quotient was 1.00 ± 0.07 (mean ± sem), for the euthyroid patient group (n = 21) 1.12 ± 0.05 and for the healthy control group (n = 21) 1.37 ± 0.08. There was a significant difference between the quotients for the control group and the hyperthyroid (P < 0.01) as well as the euthyroid (P < 0.05) patient group. The quotients for the two groups of patients did not differ significantly. In conclusion, the present study supports the view of a defect in suppressor T lymphocyte function in patients with Graves' disease in the hyperthyroid state and indicates that this defect can persist in the euthyroid state after treatment.

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The roles of CD8 in cytotoxic T lymphocyte function

Immunology Today ◽

10.1016/0167-5699(93)90283-q ◽

1993 ◽

Vol 14 (4) ◽

pp. 177-183 ◽

Cited By ~ 44

Author(s):

Anne M. O'Rourke ◽

Matthew F. Mescher

Keyword(s):

T Lymphocyte ◽

Cytotoxic T Lymphocyte ◽

Lymphocyte Function

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Differential impact of the transcriptional repressor Gfi1 on mature CD4+ and CD8+ T lymphocyte function

European Journal of Immunology ◽

10.1002/eji.200737130 ◽

2007 ◽

Vol 37 (12) ◽

pp. 3551-3563 ◽

Cited By ~ 24

Author(s):

Denise Pargmann ◽

Raif Yücel ◽

Christian Kosan ◽

Ingrid Saba ◽

Ludger Klein-Hitpass ◽

...

Keyword(s):

T Lymphocyte ◽

Transcriptional Repressor ◽

Lymphocyte Function ◽

Differential Impact ◽

Cd8 T Lymphocyte

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Melanoma exosomes deliver a complex biological payload that upregulates PTPN11 to suppress T lymphocyte function

Pigment Cell & Melanoma Research ◽

10.1111/pcmr.12564 ◽

2017 ◽

Vol 30 (2) ◽

pp. 203-218 ◽

Cited By ~ 17

Author(s):

Yueting Wu ◽

Wentao Deng ◽

Emily Chambers McGinley ◽

David J. Klinke

Keyword(s):

T Lymphocyte ◽

Lymphocyte Function

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Patients with Chromosome 11q-abberations are Characterized by a Combined Primary Immunodeficiency Involving Both B- and T-lymphocytes

10.21203/rs.3.rs-740083/v1 ◽

2021 ◽

Author(s):

Elisabeth Janneke Huisman ◽

A. Rick Brooimans ◽

Samone Mayer ◽

Marieke Joosten ◽

Louis de Bont ◽

...

Keyword(s):

T Lymphocyte ◽

B Lymphocyte ◽

Fungal Infections ◽

Prophylactic Antibiotic ◽

Tube Placement ◽

Primary Immune Deficiency ◽

Lymphocyte Function ◽

Chromosome 11 ◽

Infectious Burden ◽

Tract Infections

Abstract Disorders of the long arm of chromosome 11 (11q) are rare and involve various chromosomal regions. Patients with 11q-disorders, including Jacobsen syndrome, often present with a susceptibility for bacterial, prolonged viral and fungal infections partially explained by hypogammaglobulinemia. Additional T-lymphocyte or granular neutrophil dysfunction may also be present. In order to evaluate infectious burden and immunological function in patients with 11q-disorders, we prospectively studied a cohort of 14 patients with various 11q aberrations. Clinically, 12 patients exhibited prolonged and repetitive respiratory tract infections, frequently requiring (prophylactic) antibiotic treatment (n=7), ear-tube placement (n=9) or use of inhalers (n=5). Complicated varicella infections (n=5), chronic eczema (n=6), warts and chronic fungal infections (n=4) were reported. Six patients were on immunoglobulin replacement therapy. We observed a high prevalence of low B-lymphocyte counts (n=8), decreased T-lymphocyte counts (n=5) and abnormal T-lymphocyte function (n=12). Granulocyte function was abnormal in 29% without an aberrant clinical phenotype. Immunodeficiency was found in patients with terminal and interstitial 11q-deletions and in one patient with 11q trisomy. Genetically, FLI1 and ETS1 are seen as causative for the immunodeficiency, but these genes were deleted nor duplicated in 5 of our 14 patients. Alternative candidate genes on 11q such as ATM, CD3-cluster, CBL and THYN1 may have a role in immune dysregulation in our patients. In conclusion, we present evidence that a combined primary immune deficiency may be present in patients with 11q-disorders leading to clinically relevant infections. Therefore, broad immunological screening and necessary treatment is of importance in this patient group.

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Modulation of Cytotoxic T Lymphocyte Function by Cyclic 3′,5′-Mononucleotides

Cyclic AMP, Cell Growth, and the Immune Response ◽

10.1007/978-3-642-86026-3_17 ◽

1974 ◽

pp. 209-222

Author(s):

T. B. Strom ◽

A. Deisseroth ◽

J. Morganroth ◽

C. B. Carpenter ◽

J. P. Merrill

Keyword(s):

T Lymphocyte ◽

Cytotoxic T Lymphocyte ◽

Lymphocyte Function

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Lithium enhancement of megakaryocytopoiesis in culture: mediation via accessory marrow cells

Blood ◽

10.1182/blood.v62.1.172.172 ◽

1983 ◽

Vol 62 (1) ◽

pp. 172-176 ◽

Cited By ~ 18

Author(s):

C Chatelain ◽

SA Burstein ◽

LA Harker

Keyword(s):

T Lymphocyte ◽

Conditioned Media ◽

Pokeweed Mitogen ◽

Accessory Cell ◽

Lymphocyte Function ◽

Spleen Cells ◽

Hematopoietic Progenitors ◽

Marrow Cultures ◽

Marrow Cells

Abstract To examine the effect of lithium (Li) on early megakaryocytopoiesis, murine marrow megakaryocytic (CFU-M) and granulocyte-macrophage (CFU-C) progenitors were assayed in vitro with and without addition of lithium chloride (LiCl) to culture. At 2 mM LiCl, the numbers of CFU-M- and CFU- C-derived colonies were increased to 146% +/- 8% and 128% +/- 6% of controls, respectively (p less than 0.005). Enumeration of megakaryocytes per colony showed a 78% increase of colonies (p less than 0.05) containing from 6 to 22 cells, suggesting an increased proliferative capacity of CFU-M in the presence of LiCl. Conditioned media from spleen cells cultured in the presence of both pokeweed mitogen (PWM-SCM) and 2 mM Li increased the numbers of CFU-M and CFU-C to 157% +/- 8% and 183% +/- 8%, respectively (p less than 0.025), compared to control cultures stimulated by PWM-SCM alone. Since the production of active colony-stimulating activities (CSA) from mitogen- stimulated conditioned media requires T lymphocytes, we hypothesized that the enhancement of the growth of early hematopoietic progenitors in marrow cultures was due to a Li-induced CSA production by accessory marrow cells, rather than a direct effect of Li on stem cells. To test this, cyclosporin-A (CyA), a T-lymphocyte function inhibitor known to suppress CSA production in PWM-SCM, was added to marrow cultures in the presence of 2 mM Li. CyA (3 micrograms/ml) abrogated the Li-induced enhancement of CFU-M and CFU-C growth, but had no effect on colony formation when added alone. The data suggest that the Li-induced enhancement of early megakaryocytopoiesis and granulocytopoiesis is due to local production of CSA(s) by an accessory cell population and requires the integrity of T-lymphocyte function.

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The effect of desferrithiocin, an oral iron chelator, on T-cell function

Blood ◽

10.1182/blood.v76.10.2052.2052 ◽

1990 ◽

Vol 76 (10) ◽

pp. 2052-2059 ◽

Cited By ~ 20

Author(s):

BE Bierer ◽

DG Nathan

Keyword(s):

T Cells ◽

T Cell ◽

T Cell Activation ◽

T Lymphocyte ◽

Cell Function ◽

Iron Chelator ◽

Lymphocyte Function ◽

Ferrous Chloride ◽

Inhibition Of Proliferation ◽

T Cell Function

Abstract Desferrithiocin is a new, potent, orally available iron chelator. To determine whether this drug might be useful not only for iron-overload but also for immunosuppression, we studied the in vitro effects of desferrithiocin on T-lymphocyte function. Like deferoxamine, desferrithiocin inhibited, in a dose-dependent fashion, mitogen- and lectin-induced proliferation of both human and murine T cells. It was active at a concentration of 10 micrograms/mL. The inhibition of proliferation was reversed by ferrous chloride, but not by other metal salts, recombinant IL-2, or conditioned medium. Desferrithiocin also inhibited proliferation of constitutively dividing, and factor- independent EBV-transformed B cell and leukemic T-cell lines. Although desferrithiocin inhibited the induction of cytotoxic T lymphocyte (CTL) activity, it did not inhibit CTL- or natural killer-induced cytotoxicity. The agent did not inhibit the expression of activation antigens such as the IL-2 receptor on T cells, nor early measures of T- cell activation such as the influx of intracellular calcium. Thus, desferrithiocin, like deferoxamine, is a potent and reversible inhibitor of T-cell proliferation. This anti-proliferative effect inhibits T-cell function. Bioavailability after oral administration is a unique property of desferrithiocin, and would make it an attractive alternative to deferoxamine. Its immunomodulating properties may therefore be exploited in vivo to inhibit graft rejection or autoreactive T cells.

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