scholarly journals Flexibility in the critical period of nutrient sequestration in bumble bee queens

2021 ◽  
Author(s):  
Kristal M Watrous ◽  
Claudinéia Costa ◽  
Yadira R Diaz ◽  
S Hollis Woodard
Keyword(s):  
Life Stage ◽  
Food Resource ◽  
Storage Period ◽  
Resource Limitation ◽  
Food Resources ◽  
Bumble Bees ◽  
Bumble Bee ◽  
Nutrient Storage ◽  
Adequate Food ◽  
Nutrient Sequestration

Abstract Bumble bee queens undergo a nutrient storage period prior to entering diapause wherein they sequester glycogen and lipids that are metabolized during overwintering. In the laboratory under optimal food availability conditions, the majority of nutrients are sequestered during the first few days of adulthood. However, if food resources are scarce during this narrow window of time, wild queen bumble bees might be limited in their ability to obtain adequate food resources for overwintering. Here we used a laboratory experiment to examine whether queen bumble bees exhibit flexibility in the timing of pre-overwintering nutrient sequestration, by limiting their access to either nectar (artificial) or pollen, the two primary foods for bumble bees, for varying periods of time. In response to these treatments, we quantified queen survival, changes in weight, and glycogen and lipids levels. We found evidence that queens are able to recuperate almost entirely from food resource limitation, with respect to nutrient storage, especially when it is experienced for shorter durations (up to 6 days). This study sheds light on how bumble bee queens are impacted by food resource availability at a critical life stage.

scholarly journals Diet and nutritional status during early adult life have immediate and persistent effects on queen bumble bees

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
S Hollis Woodard ◽  
Michelle A Duennes ◽  
Kristal M Watrous ◽  
Shalene Jha
Keyword(s):  
Life History ◽  
Nutritional Status ◽  
Adult Life ◽  
Storage Period ◽  
Primary Source ◽  
Bumble Bees ◽  
Sugar Concentration ◽  
Early Adult Life ◽  
Nectar Sugar ◽  
Nutrient Sequestration

Abstract Many insects sequester nutrients during developmentally programmed periods, which they metabolize during subsequent life history stages. During these periods, failure to store adequate nutrients can have persistent effects on fitness. Here, we examined a critical but under-studied nutrient storage period in queen bumble bees: the first days of adult life, which are followed by a diapause period typically coinciding with winter. We experimentally manipulated availability of pollen (the primary dietary source of lipids and protein) and the sugar concentration of artificial nectar (the primary source of carbohydrates) for laboratory-reared queens during this period and examined three nutritional phenomena: (i) diet impacts on nutritional status, (ii) the timescale upon which nutrient sequestration occurs and (iii) the fitness consequences of nutrient sequestration, specifically related to survival across the life cycle. We found evidence that pollen and nectar starvation negatively impact lipid storage, whereas nectar sugar concentration impacts stored carbohydrates. The majority of nutrients were stored during the first ~ 3 days of adult life. Nutrients derived from pollen during this period appear to be more critical for surviving earlier life stages, whereas nutrients sequestered from nectar become more important for surviving the diapause and post-diapause periods. Negative impacts of a poor diet during early life persisted in our experiment, even when pollen and a relatively high (50%) nectar sugar concentration were provided post-diapause. Based on these findings, we posit that the nutritional environment during the early adult life of queens has both immediate and persistent impacts on fitness. These findings underscore the importance of examining effects of stage-specific nutritional limitations on physiology and life history traits in this social insect group. Moreover, the findings may shed light on how declining food resources are contributing to the decline of wild bumble bee populations.

scholarly journals Landscape Enhancements in Apple Orchards: Higher Bumble Bee Queen Species Richness, but No Effect on Apple Quality

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 421
Author(s):  
Amélie Gervais ◽  
Marc Bélisle ◽  
Marc J. Mazerolle ◽  
Valérie Fournier
Keyword(s):  
Species Richness ◽  
Bumble Bees ◽  
Bumble Bee ◽  
Pesticide Use ◽  
Apple Orchards ◽  
Seed Number ◽  
Apple Production ◽  
Apple Quality ◽  
Effective Pollinators ◽  
Quality Weight

Bumble bees are among the most effective pollinators in orchards during the blooming period, yet they are often threatened by the high levels of pesticide use in apple production. This study aimed to evaluate the influence of landscape enhancements (e.g., hedgerows, flower strips) on bumble bee queens in apple orchards. Bumble bee queens from 12 orchards in southern Québec (Canada) were marked, released, and recaptured in the springs and falls of 2017 to 2019. Half of the 12 orchards had landscape enhancements. Apples were harvested in 2018 and 2019 to compare their quality (weight, diameter, sugar level, and seed number) in sites with and without landscape enhancements. Species richness, as well as the occurrence of three species out of eight, was higher in orchards with landscape enhancements than in orchards without such structures. The occurrence of Bombus ternarius was lower in orchards with high levels of pesticide use. Apples had fewer seeds when collected in orchards with landscape enhancements and were heavier in orchards that used more pesticides. Our work provides additional evidence that landscape enhancements improve bumble bee presence in apple orchards and should therefore be considered as a means to enhance pollination within farms.

scholarly journals Olfactory coding in the antennal lobe of the bumble bee Bombus terrestris

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcel Mertes ◽  
Julie Carcaud ◽  
Jean-Christophe Sandoz
Keyword(s):  
Honey Bees ◽  
Antennal Lobe ◽  
Bombus Terrestris ◽  
Bumble Bees ◽  
Foraging Strategies ◽  
Bumble Bee ◽  
Carbon Chain Length ◽  
Olfactory Coding ◽  
Major Transitions

AbstractSociality is classified as one of the major transitions in evolution, with the largest number of eusocial species found in the insect order Hymenoptera, including the Apini (honey bees) and the Bombini (bumble bees). Bumble bees and honey bees not only differ in their social organization and foraging strategies, but comparative analyses of their genomes demonstrated that bumble bees have a slightly less diverse family of olfactory receptors than honey bees, suggesting that their olfactory abilities have adapted to different social and/or ecological conditions. However, unfortunately, no precise comparison of olfactory coding has been performed so far between honey bees and bumble bees, and little is known about the rules underlying olfactory coding in the bumble bee brain. In this study, we used in vivo calcium imaging to study olfactory coding of a panel of floral odorants in the antennal lobe of the bumble bee Bombus terrestris. Our results show that odorants induce reproducible neuronal activity in the bumble bee antennal lobe. Each odorant evokes a different glomerular activity pattern revealing this molecule’s chemical structure, i.e. its carbon chain length and functional group. In addition, pairwise similarity among odor representations are conserved in bumble bees and honey bees. This study thus suggests that bumble bees, like honey bees, are equipped to respond to odorants according to their chemical features.

scholarly journals Diet seasonality and food overlap of the fish assemblage in a pantanal pond

2008 ◽  
Vol 6 (4) ◽  
pp. 567-576 ◽  
Author(s):  
Gisele Caroline Novakowski ◽  
Norma Segatti Hahn ◽  
Rosemara Fugi
Keyword(s):  
Fish Assemblage ◽  
Food Resource ◽  
Fish Fauna ◽  
Diet Breadth ◽  
Food Resources ◽  
Dry Season ◽  
Trophic Guilds ◽  
Trophic Specialization ◽  
Mato Grosso ◽  
Mean Values

We assessed the trophic structure of the fish fauna in Sinhá Mariana pond, Mato Grosso State, from March 2000 to February 2001. The aim was to determine the feeding patterns of the fish species during the rainy and dry seasons. The diets of 26 species (1,294 stomach contents) were determined by the volumetric method. Insects and fish were the most important food resources: insects were the dominant food of 23% and 27% of the species, respectively, in the rainy and dry season, and fish was the dominant item for 31% of the species in both seasons. Cluster analysis (Euclidean Distance) identified seven trophic guilds in the rainy season (detritivores, herbivores, insectivores, lepidophages, omnivores, piscivores and planktivores), and five trophic guilds in the dry season (detritivores, insectivores, lepidophages, omnivores and piscivores). The smallest mean values of diet breadth were observed for the specialist guilds (detritivores, lepidophages and piscivores), in both seasons. The widest means for diet breadth were observed for the omnivores, regardless of the season. In general, there was no seasonal variation in feeding overlap among the species studied. At the community level, diet overlap values between species were low (< 0.4) for 80% of the pairs in each season, suggesting wide partitioning of the food resource. The fish assemblage showed a tendency toward trophic specialization, regardless of the season, although several species changed their diets. We might consider two non-excludent hypothesis: that there is no pattern on the use of seasonal food resources and/or probably there are several patterns, because each one is based on characteristics of the studied site and the taxonomic composition of the resident species.

scholarly journals The influence of conopid flies on bumble bee colony productivity under different food resource conditions

2018 ◽  
Vol 88 (4) ◽  
pp. 653-671 ◽  
Author(s):  
Rosemary L. Malfi ◽  
Jonathan A. Walter ◽  
T'ai H. Roulston ◽  
Clara Stuligross ◽  
Sarah McIntosh ◽  
...  
Keyword(s):  
Food Resource ◽  
Bumble Bee ◽  
Bee Colony ◽  
Resource Conditions ◽  
Colony Productivity

First Complete Genome of Lake Sinai Virus Lineage 3 and Genetic Diversity of Lake Sinai Virus Strains From Honey Bees and Bumble Bees

2020 ◽  
Vol 113 (3) ◽  
pp. 1055-1061 ◽  
Author(s):  
Laura Šimenc ◽  
Urška Kuhar ◽  
Urška Jamnikar-Ciglenečki ◽  
Ivan Toplak
Keyword(s):  
Honey Bee ◽  
Honey Bees ◽  
Complete Genome ◽  
Bumble Bees ◽  
Bumble Bee ◽  
Rt Pcr ◽  
Genome Region ◽  
Pcr Method ◽  
Next Generation Sequencing Ngs ◽  
First Time

Abstract The complete genome of Lake Sinai virus 3 (LSV3) was sequenced by the Ion Torrent next-generation sequencing (NGS) technology from an archive sample of honey bees collected in 2010. This strain M92/2010 is the first complete genome sequence of LSV lineage 3. From October 2016 to December 2017, 56 honey bee samples from 32 different locations and 41 bumble bee samples from five different locations were collected. These samples were tested using a specific reverse transcriptase-polymerase chain reaction (RT-PCR) method; 75.92% of honey bee samples and 17.07% of bumble bee samples were LSV-positive with the RT-PCR method. Phylogenetic comparison of 557-base pair-long RNA-dependent RNA polymerase (RdRp) genome region of selected 23 positive samples of honey bees and three positive bumble bee samples identified three different LSV lineages: LSV1, LSV2, and LSV3. The LSV3 lineage was confirmed for the first time in Slovenia in 2010, and the same strain was later detected in several locations within the country. The LSV strains detected in bumble bees are from 98.6 to 99.4% identical to LSV strains detected among honey bees in the same territory.

scholarly journals Why Are Queens Broodless? Failed Nest Initiation Not Linked to Parasites, Mating Status, or Ovary Development in Two Bumble Bee Species of Pyrobombus (Hymenoptera: Apidae: Bombus)

2019 ◽  
Vol 113 (2) ◽  
pp. 575-581 ◽  
Author(s):  
Jessica L Mullins ◽  
James P Strange ◽  
Amber D Tripodi
Keyword(s):  
North America ◽  
Commercial Production ◽  
Bumble Bees ◽  
Ovary Development ◽  
Bumble Bee ◽  
Agricultural Crops ◽  
Western North America ◽  
Mating Status ◽  
Pollination Services ◽  
Causal Factors

Abstract Bumble bees (Bombus [Hymenoptera: Apidae]) are important pollinators for agricultural crops, which has led to their commercial domestication. Despite their importance, little is known about the reproductive biology of bumble bees native to North America. The Hunt bumble bee (Bombus huntii Greene [Hymenoptera: Apidae]) and the Vosnesensky bumble bee (Bombus vosnesenskii Radoszkowski [Hymenoptera: Apidae] are native candidates for commercial production in western North America due to their efficacy in providing commercial pollination services. Availability of pollinators native to the region in which services would be provided would minimize the likelihood of introducing exotic species and spreading novel disease. Some parasites are known to affect bumble bee reproduction, but little is known about their prevalence in North America or how they affect queen success. Only 38% of wild-caught B. huntii and 51% wild-caught B. vosnesenskii queens collected between 2015 and 2017 initiated nests in the laboratory. Our objective was to identify causal factors leading to a queen’s inability to oviposit. To address this, we dissected each broodless queen and diagnosed diseases, assessed mating status, and characterized ovary development. Nematodes, arthropods, and microorganisms were detected in both species. Overall, 20% of queens were infected by parasites, with higher rates in B. vosnesenskii. Over 95% of both species were mated, and over 88% had developed ovaries. This suggests that parasitism and mating status were not primary causes of broodlessness. Although some failure to nest can be attributed to assessed factors, additional research is needed to fully understand the challenges presented by captive rearing.

Freshwater Mussels as a Prehistoric Food Resource

1974 ◽  
Vol 39 (3) ◽  
pp. 421-434 ◽  
Author(s):  
Paul W. Parmalee ◽  
Walter E. Klippel
Keyword(s):  
Mississippi River ◽  
Freshwater Mussels ◽  
Food Resource ◽  
Food Resources ◽  
Archaeological Sites ◽  
Eastern North America ◽  
Subsistence Economies ◽  
Level Data ◽  
Food Potential ◽  
Prehistoric Subsistence

AbstractThe explication of prehistoric subsistence economies has been of increasing interest to archaeologists. This emphasis is reflected, in part, by the considerable attention afforded faunal analyses in the recent archaeological literature. However, little effort has been made toward developing methods that permit meaningful comparisons of food resources at the interspecies level. Data pertinent to the calculation of both quantitative and qualitative food potential of many of the vertebrate animals represented in archaeological contexts are extant. These data are lacking for freshwater mussels, frequently found in archaeological sites in eastern North America. Information relevant to determining the food energy provided by freshwater mussels common to much of the Mississippi River drainage is presented. Results are applicable to more credible interpretations of prehistoric subsistence as related to animal food resources.

Stories for living on a damaged planet: Environmental education in a preschool classroom

2017 ◽  
Vol 16 (2) ◽  
pp. 148-159 ◽  
Author(s):  
Fikile Nxumalo
Keyword(s):  
British Columbia ◽  
Early Childhood ◽  
Environmental Education ◽  
Scientific Knowledge ◽  
Bumble Bees ◽  
Bumble Bee ◽  
Anthropogenic Change ◽  
Early Childhood Classroom ◽  
Preschool Classroom

This article examines children’s encounters with dead and dying bumble bees in their everyday entangled lives. Within the context of an early childhood classroom located in suburban British Columbia, Canada, the article stories situated and emergent bee–child worldings to illustrate possibilities for learning with other species in anthropogenically damaged worlds. I pay attention to some of the ways in which children’s and educators’ practices have shifted away from encountering bees predominantly as objects of scientific knowledge towards more relational, embodied, and affective immersion in the lives and deaths of bumble bees. Situating these practices within current bumble bee vulnerabilities, I consider how children’s and educators’ inquiries might be viewed as pedagogies that matter for learning to live less destructively with others in current times of anthropogenic change.

Diversity, density, efficiency, and effectiveness of pollinators of cicer milkvetch, Astragalus cicer L.

1987 ◽  
Vol 65 (9) ◽  
pp. 2168-2176 ◽  
Author(s):  
K. W. Richards
Keyword(s):  
Honey Bee ◽  
Honey Bees ◽  
Propagation Rate ◽  
Bumble Bees ◽  
Bumble Bee ◽  
Bee Pollination ◽  
Southern Alberta ◽  
Efficiency And Effectiveness ◽  
Astragalus Cicer

Diversity, density, efficiency, and effectiveness of pollinators of cicer milkvetch, Astragalus cicer L., grown at two locations in southern Alberta were studied from 1978 to 1983. Twenty-seven species of bees were identified as pollinators. At Lethbridge, honey bees (Apis mellifera) comprised 74% of the observations, bumble bees 16%, and leafcutter bees 10%, while at Spring Coulee, the proportions were honey bees 14%, bumble bees 69%, and leafcutter bees 17%. The rate of foraging by pollinator species from flower to flower varied; bumble bee species, especially Bombus nevadensis Cress., foraged consistently more efficiently than honey bees or alfalfa leafcutter bees, Megachile rotundata (F.). A theoretical approach used to predict the bee populations required to pollinate varying flower densities shows that the population of B. nevadensis required is about half those of Bombus huntii Greene and M. rotundata and less than one-quarter that of the honey bee. Pollination by B. nevadensis consistently resulted in more seeds per pod than with any other bumble bee species, the honey bee, or M. rotundata. Of the nine species of bumble bee that established colonies in artificial domiciles near the field, B. nevadensis established the most colonies each year. The number of workers and sexuals produced per colony varied considerably among bumble bee species with only 55% of the colony establishments producing workers and 31% producing sexuals. The propagation rate and quality of alfalfa leafcutter bees produced on cicer milkvetch was excellent.

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