scholarly journals Characterisation and molecular cloning of the novel macrolide-streptogramin B resistance determinant from Staphylococcus epidermidis

1989 ◽  
Vol 24 (6) ◽  
pp. 851-862 ◽  
Author(s):  
Jeremy I. Ross ◽  
Angela M. Farrell ◽  
E. Anne Eady ◽  
Jonathan H. Cove ◽  
William J. Cunliffe
Keyword(s):  
Molecular Cloning ◽  
Staphylococcus Epidermidis ◽  
Resistance Determinant ◽  
The Novel

Purification, partial characterization and molecular cloning of the novel antiviral protein RC28

Peptides ◽  
2009 ◽  
Vol 30 (4) ◽  
pp. 654-659 ◽  
Author(s):  
Meng Gong ◽  
Frank Piraino ◽  
Naihong Yan ◽  
Jing Zhang ◽  
Minjie Xia ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Partial Characterization ◽  
The Novel ◽  
Antiviral Protein

Molecular cloning and characterization of the novel acidic xylanase XYLD from Bispora sp. MEY-1 that is homologous to family 30 glycosyl hydrolases

2010 ◽  
Vol 86 (6) ◽  
pp. 1829-1839 ◽  
Author(s):  
Huiying Luo ◽  
Jun Yang ◽  
Jiang Li ◽  
Pengjun Shi ◽  
Huoqing Huang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
The Novel ◽  
Glycosyl Hydrolases

scholarly journals Feleucins: Novel Bombinin Precursor-Encoded Nonapeptide Amides from the Skin Secretion ofBombina variegata

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Bing Bai ◽  
Xiaojuan Hou ◽  
Lei Wang ◽  
Lilin Ge ◽  
Yu Luo ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Present Report ◽  
Single Copy ◽  
Open Reading Frames ◽  
The Novel ◽  
Skin Secretion ◽  
Amphibian Skin ◽  
Novel Structure ◽  
Bombina Variegata ◽  
Weak Activity

The first amphibian skin antimicrobial peptide (AMP) to be identified was named bombinin, reflecting its origin from the skin of the European yellow-bellied toad (Bombina variegata). Bombinins and their related peptides, the bombinin Hs, were subsequently reported from other bombinid toads. Molecular cloning of bombinin-encoding cDNAs from skin found that bombinins and bombinin Hs were coencoded on the same precursor proteins. Here, we report the molecular cloning of two novel cDNAs from a skin secretion-derived cDNA library ofB. variegatawhose open-reading frames each encode a novel bombinin (GIGGALLNVGKVALKGLAKGLAEHFANamide) and a C-terminally located single copy of a novel nonapeptide (FLGLLGGLLamide or FLGLIGSLLamide). These novel nonapeptides were named feleucin-BV1 and feleucin-BV2, respectively. The novel bombinin exhibited 89% identity to homologues from the toads,B. microdeladigitoraandB. maxima. The feleucins exhibited no identity with any amphibian AMP archived in databases. Synthetic feleucins exhibited a weak activity againstStaphylococcus aureus(128–256 mg/L) but feleucin-BV1 exhibited a synergistic action with the novel bombinin. The present report clearly demonstrates that the skin secretions of bombinid toads continue to represent a source of peptides of novel structure that could provide templates for the design of therapeutics.

Molecular cloning and functional analysis of a novel macrolide‐resistance determinant, mefA , from Streptococcus pyogenes

1996 ◽  
Vol 22 (5) ◽  
pp. 867-879 ◽  
Author(s):  
Joanna Clancy ◽  
Joan Petitpas ◽  
Fadia Dib‐Hajj ◽  
Wei Yuan ◽  
Melissa Cronan ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Cloning ◽  
Streptococcus Pyogenes ◽  
Macrolide Resistance ◽  
Resistance Determinant

Molecular Cloning and Expression of the Novel Splice Variants of K+ Channel-Interacting Protein 2

2001 ◽  
Vol 282 (1) ◽  
pp. 96-102 ◽  
Author(s):  
Susumu Ohya ◽  
Yuichi Morohashi ◽  
Katsuhiko Muraki ◽  
Taisuke Tomita ◽  
Minoru Watanabe ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Splice Variants ◽  
Cloning And Expression ◽  
K Channel ◽  
The Novel ◽  
Interacting Protein

scholarly journals A Novel, WidespreadqacAAllele Results in Reduced Chlorhexidine Susceptibility inStaphylococcus epidermidis

2019 ◽  
Vol 63 (6) ◽  
Author(s):  
Amin Addetia ◽  
Alexander L. Greninger ◽  
Amanda Adler ◽  
Shuhua Yuan ◽  
Negar Makhsous ◽  
...  
Keyword(s):  
Amino Acid ◽  
Staphylococcus Epidermidis ◽  
Sequence Data ◽  
Methicillin Resistance ◽  
Single Amino Acid ◽  
Data Sets ◽  
The Novel ◽  
Content Type

ABSTRACTChlorhexidine gluconate (CHG) is a topical antiseptic widely used in health care settings. InStaphylococcusspp., the pump QacA effluxes CHG, while the closely related QacB cannot due to a single amino acid substitution. We characterized 1,050 cutaneousStaphylococcusisolates obtained from 173 pediatric oncology patients enrolled in a multicenter CHG bathing trial. CHG susceptibility testing revealed that 63 (6%) of these isolates had elevated CHG MICs (≥4 μg/ml). Screening of all 1,050 isolates for theqacA/Bgene (the sameqacgene with A or B allele) by restriction fragment length polymorphism (RFLP) yielded 56 isolates with a novelqacA/BRFLP pattern,qacA/B273. The CHG MIC was significantly higher forqacA/B273-positive isolates (MIC50, 4 μg/ml; MIC range, 0.5 to 4 μg/ml) than for otherqacgroups:qacA-positive isolates (n = 559; MIC50, 1 μg/ml; MIC range, 0.5 to 4 μg/ml),qacB-positive isolates (n = 17; MIC50, 1 μg/ml; MIC range, 0.25 to 2 μg/ml), andqacA/B-negative isolates (n = 418, MIC50, 1 μg/ml; MIC range, 0.125 to 2 μg/ml) (P = 0.001). A high proportion of theqacA/B273-positive isolates also displayed methicillin resistance (96.4%) compared to the otherqacgroups (24.9 to 61.7%) (P = 0.001). Whole-genome sequencing revealed thatqacA/B273-positive isolates encoded a variant of QacA with 2 amino acid substitutions. This new allele, namedqacA4, was carried on the novel plasmid pAQZ1. TheqacA4-carrying isolates belonged to the highly resistantStaphylococcus epidermidissequence type 2 clone. By searching available sequence data sets, we identified 39 additionalqacA4-carryingS. epidermidisstrains from 5 countries. Curing an isolate ofqacA4resulted in a 4-fold decrease in the CHG MIC, confirming the role ofqacA4in the elevated CHG MIC. Our results highlight the importance of further studyingqacA4and its functional role in clinical staphylococci.

Sign in / Sign up

Export Citation Format

Share Document