An NDM-1-producing Escherichia coli obtained in Denmark has a genetic profile similar to an NDM-1-producing E. coli isolate from the UK

SummaryWe previously found that human pro-UK expressed in Escherichia coli is more active in fibrinolysis than recombinant human pro-UK obtained from mammalian cell culture media. To determine whether this difference is related to the lack of glycosylation of the E. coli product, we compared the activity of E. coli-derived pro-UK [(-)pro-UK] with that of a glycosylated pro-UK [(+)pro-UK] and of a mutant of pro-UK missing the glycosylation site at Asn-302 [(-) (302) pro-UK]. The latter two pro-UKs were obtained by expression of the human gene in a mammalian cell. The nonglycosylated pro-UKs were activated by plasmin more efficiently (≈2-fold) and were more active in clot lysis (1.5-fold) than the (+)pro-UK. Similarly, the nonglycosylated two-chain derivatives (UKs) were more active against plasminogen and were more rapidly inactivated by plasma inhibitors than the (+)UK.These findings indicate that glycosylation at Asn-302 influences the activity of pro-UK/UK and could be the major factor responsible for the enhanced activity of E. coli-derived pro-UK.

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First Report and Comparative Genomics Analysis of a blaOXA-244–Harbouring Escherichia coli Isolate Recovered in American Continent

10.20944/preprints201909.0150.v1 ◽

2019 ◽

Author(s):

Deisy J Abril ◽

Ingrid Gisell Bustos Moya ◽

Ricaurte Alejandro Marquez-Ortiz ◽

Diego Fernando Josa Montero ◽

Zayda Lorena Corredor Rozo ◽

...

Keyword(s):

Escherichia Coli ◽

Comparative Genomics ◽

Genomic Island ◽

Genomic Analysis ◽

American Continent ◽

Incompatibility Group ◽

Coli Isolate ◽

First Report ◽

E Coli ◽

Comparative Genomics Analysis

The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harbouring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harbouring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38 and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbour resistance genes. The blaOXA-244 gene was chromosomally-encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. For our knowledge this is the first report of a blaOXA-244–harbouring Escherichia coli isolate in American continent.Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently on the American continent.

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CTX-M-15 Extended-Spectrum β-Lactamase in a Shiga Toxin-Producing Escherichia coli Isolate of Serotype O111:H8

Applied and Environmental Microbiology ◽

10.1128/aem.06997-11 ◽

2011 ◽

Vol 78 (4) ◽

pp. 1308-1309 ◽

Cited By ~ 29

Author(s):

Charlotte Valat ◽

Marisa Haenni ◽

Estelle Saras ◽

Frédéric Auvray ◽

Karine Forest ◽

...

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Content Type ◽

Coli Isolate ◽

E Coli ◽

Extended Spectrum

ABSTRACTWe report the discovery of a CTX-M-15-producingEscherichia coli(STEC) of serogroup O111:H8, a major serotype responsible for human enterohemorrhagicEscherichia coli(EHEC) infections. In line with the recent CTX-M-15/O104:H4E. colioutbreak, these data may reflect an accelerating spread of resistance to expanded-spectrum cephalosporins within theE. colipopulation, including STEC isolates.

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Identification of a Novel bla NDM Variant, bla NDM-33, in an Escherichia coli Isolate from Hospital Wastewater in China

mSphere ◽

10.1128/msphere.00776-21 ◽

2021 ◽

Author(s):

Tao Wang ◽

Yuan Zhou ◽

Chunhong Zou ◽

Zhichen Zhu ◽

Jie Zhu ◽

...

Keyword(s):

Escherichia Coli ◽

Human Disease ◽

Antibiotic Exposure ◽

Hospital Wastewater ◽

Content Type ◽

Coli Isolate ◽

E Coli ◽

Hospital Sewage

Our study described a novel NDM-33 variant from an E. coli strain isolated from hospital sewage, where it was associated with human disease and antibiotic exposure. Importantly, hospital sewage was increasingly considered to be related to CRE hosts.

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Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis

Microbiology ◽

10.1099/mic.0.27448-0 ◽

2004 ◽

Vol 150 (11) ◽

pp. 3631-3645 ◽

Cited By ~ 141

Author(s):

Francis Dziva ◽

Pauline M. van Diemen ◽

Mark P. Stevens ◽

Amanda J. Smith ◽

Timothy S. Wallis

Keyword(s):

Escherichia Coli ◽

Molecular Mechanisms ◽

Minor Role ◽

Intestinal Colonization ◽

E Coli ◽

Type Iii ◽

Fimbrial Subunit ◽

Type Iii Protein Secretion ◽

The Uk ◽

A Minor

Enterohaemorrhagic Escherichia coli (EHEC) cause acute gastroenteritis in humans that may be complicated by life-threatening systemic sequelae. The predominant EHEC serotype affecting humans in the UK and North America is O157 : H7 and infections are frequently associated with contact with ruminant faeces. Strategies to reduce the carriage of EHEC in ruminants are expected to lower the incidence of human EHEC infections; however, the molecular mechanisms underlying persistence of EHEC in ruminants are poorly understood. This paper reports the first comprehensive survey for EHEC factors mediating colonization of the bovine intestines by using signature-tagged transposon mutagenesis. Seventy-nine E. coli O157 : H7 mutants impaired in their ability to colonize calves were isolated and 59 different genes required for intestinal colonization were identified by cloning and sequencing of the transposon insertion sites. Thirteen transposon insertions were clustered in the locus of enterocyte effacement (LEE), which encodes a type III protein secretion system required for the formation of attaching and effacing lesions on intestinal epithelia. A putative structural component of the apparatus (EscN) is essential for intestinal colonization; however, the type III secreted effector protein Map plays only a minor role. Other Type III secretion-associated genes were implicated in colonization of calves by E. coli O157 : H7, including z0990 (ecs0850), which encodes the non-LEE-encoded type III secreted effector NleD and the closely related z3023 (ecs2672) and z3026 (ecs2674) genes which encode homologues of Shigella IpaH proteins. We also identified a novel fimbrial locus required for intestinal colonization in calves by E. coli O157 : H7 (z2199-z2206; ecs2114-ecs2107/locus 8) and demonstrated that a mutant harbouring a deletion of the putative major fimbrial subunit gene is rapidly out-competed by the parent strain in co-infection studies. Our data provide valuable new information for the development of intervention strategies.

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ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dky484 ◽

2018 ◽

Vol 74 (3) ◽

pp. 557-560 ◽

Cited By ~ 14

Author(s):

Louise Roer ◽

Søren Overballe-Petersen ◽

Frank Hansen ◽

Thor B Johannesen ◽

Marc Stegger ◽

...

Keyword(s):

Escherichia Coli ◽

Bloodstream Infection ◽

Coli Isolate ◽

E Coli

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Genotypic and phenotypic characterization of attaching and effacing Escherichia coli (AEEC) isolated from children with and without diarrhoea in Londrina, Brazil

Journal of Medical Microbiology ◽

10.1099/jmm.0.04977-0 ◽

2003 ◽

Vol 52 (6) ◽

pp. 499-504 ◽

Cited By ~ 13

Author(s):

Eliane Blanco Nunes ◽

Halha Ostrensky Saridakis ◽

Kinue Irino ◽

Jacinta Sanchez Pelayo

Keyword(s):

Escherichia Coli ◽

Strong Association ◽

Phenotypic Characterization ◽

Genetic Profile ◽

Phenotypic Characteristics ◽

Common Pattern ◽

E Coli ◽

Genetic Profiles ◽

The City

Attaching and effacing Escherichia coli (AEEC) have been implicated in diarrhoea in humans in several countries. A total of 919 E. coli strains, isolated from 125 children with diarrhoea and 98 without diarrhoea, was investigated by PCR for the presence of the EAF, bfp, eae and stx genes. Thirty-four of these isolates were found to carry the eae gene; they were isolated from 27 (79.4 %) children with diarrhoea and seven (20.6 %) controls, in the city of Londrina, Brazil. These strains were investigated for their genotypic and phenotypic characteristics. Different genetic profiles were observed; strains containing the eae gene alone were most common (47.1 %). The characteristic genetic profile of typical enteropathogenic E. coli (EPEC), eae, bfp and EAF, was only found in isolates from children with diarrhoea. The stx gene was not detected in any of the 34 strains studied. Ten (29.4 %) strains were negative in the fluorescent actin-staining test. Localized adhesion (LA) was the most common pattern of adhesion (44.1 %), followed by the aggregative adhesion (AA) (23.5 %) and localized adhesion-like (LAL) (14.7 %) patterns. The results showed a strong association between strains presenting the LA pattern and diarrhoea. Forty-seven per cent of the strains studied belonged to classical O-serogroups of EPEC. The most common serotype found was O119 : H6; these isolates all showed the LA pattern, were positive for fluorescent actin-staining and were associated with diarrhoea. Intimin β was detected in seven strains, four of which belonged to serotype O119 : H6 and three to serotype ONT : H7; all were associated with diarrhoea. On the other hand, intimin ϵ was detected in two strains of serotype O111 : H38 and one of serotype ONT : H19, isolated from children without diarrhoea. To our knowledge, this is the first report of the occurrence of intimin ϵ in strains of E. coli isolated from humans in Brazil.

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Monoclonal Antibodies to Lipopolysaccharide O Antigens of Enterohemorrhagic Escherichia coli Strains in Serogroups O26, O45, O103, O111, O121, and O145

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-597 ◽

2015 ◽

Vol 78 (7) ◽

pp. 1252-1258 ◽

Cited By ~ 1

Author(s):

BRIAN W. BROOKS ◽

CHERYL L. LUTZE-WALLACE ◽

BURTON BLAIS ◽

MARTINE GAUTHIER ◽

MYLÈNE DESCHÊNES

Keyword(s):

Escherichia Coli ◽

Monoclonal Antibodies ◽

Enterohemorrhagic Escherichia Coli ◽

Human Pathogens ◽

Specific Detection ◽

Coli Isolate ◽

E Coli ◽

O Antigens

Non-O157 enterohemorrhagic Escherichia coli in priority serogroups O26, O45, O103, O111, O121, and O145 are increasingly recognized as important human pathogens. In the present study, a panel of monoclonal antibodies (MAbs) to the lipopolysaccharide O antigens of E. coli in serogroups O26, O45, O103, O111, O121, and O145 was produced. The specificity was evaluated by examining the reactivity of the MAbs with 50 E. coli strains and 42 non–E. coli bacteria, and several MAbs highly specific for E. coli strains in each of the six non-O157 priority serogroups were identified. The use of these highly specific MAbs may be of considerable value for determining whether an E. coli isolate belongs to one of the six priority non-O157 serogroups, for developing specific detection assays for these organisms, and for characterizing the lipopolysaccharide O antigens of isolates in these serogroups.

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Extraintestinal Pathogenic Escherichia Coli-Induced Pneumonia in Three Kittens and Fecal Prevalence in a Clinically Healthy Cohort Population

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870902100504 ◽

2009 ◽

Vol 21 (5) ◽

pp. 609-615 ◽

Cited By ~ 10

Author(s):

Margaret A. Highland ◽

Barbara A. Byrne ◽

Chitrita DebRoy ◽

Eileen M. Samitz ◽

Tracy S. Peterson ◽

...

Keyword(s):

Escherichia Coli ◽

Pulsed Field Gel Electrophoresis ◽

Genetic Profile ◽

Chain Reaction ◽

E Coli ◽

Pathogenic Escherichia Coli ◽

Hemorrhagic Pneumonia ◽

Acute Necrotizing ◽

Healthy Cohort ◽

Polymerase Chain

Three kittens, ages 5, 9, and 17 weeks, were found dead by separate caregivers and were submitted for necropsy. At gross necropsy, each kitten had hemorrhagic or bloody fibrinoserous thoracic fluid and differing distributions of pulmonary consolidation. On histologic examination, the pulmonary lesion in each kitten was similar and was characterized by acute necrotizing and hemorrhagic pneumonia and pleuritis, with numerous intralesional small Gram-negative rods. A pure culture of a distinct serotype of Escherichia coli was identified in lung tissue from each kitten (O4:H5, O6:H7, O6:H5). Lung isolates, genotyped by polymerase chain reaction, carried genes that are characteristic of extraintestinal pathogenic E. coli (ExPEC), including cnf-1, papG allele I, papA, papC, sfa, fim, hlyD, malX, iroN, fyuA, kpsMII, and ompT. Escherichia coli isolates from the intestines of 2 of the kittens were 100% related to the respective lung isolate, as determined by pulsed-field gel electrophoresis. Cultures of fecal samples collected from a clinically healthy cohort population of kittens revealed 16 of 19 tested kittens (84%) to be shedding hemolytic E. coli. Ten different serotypes were identified from 43 hemolytic E. coli fecal isolates from the cohort population, each of which had a genetic profile consistent with that typical of ExPEC. To the authors’ knowledge, this is the first report to describe a cluster of isolated cases of pneumonia in kittens caused by distinct serotypes of ExPEC and to evaluate the prevalence of hemolytic E. coli carrying ExPEC-associated genes in the feces of a cohort population of kittens.

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Molecular Characterization ofblaNDM-5Carried on an IncFII Plasmid in an Escherichia coli Isolate from a Nontraveler Patient in Spain

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.04040-14 ◽

2014 ◽

Vol 59 (1) ◽

pp. 659-662 ◽

Cited By ~ 41

Author(s):

Cristina Pitart ◽

Mar Solé ◽

Ignasi Roca ◽

Angely Román ◽

Asunción Moreno ◽

...

Keyword(s):

Escherichia Coli ◽

Female Patient ◽

Urine Culture ◽

Sequence Type ◽

Content Type ◽

Pcr Screening ◽

Coli Isolate ◽

E Coli ◽

Carbapenem Resistant ◽

Indian Origin

ABSTRACTA carbapenem-resistantEscherichia coliisolate (sequence type 448 [ST448]) was recovered from a urine culture of a female patient with no recent record of traveling. PCR screening identified the presence ofblaNDM-5,blaTEM-1,blaOXA-1,blaCMY-42, andrmtB. blaNDM-5was carried in a conjugative IncFII-type plasmid (90 kb) together withblaTEM-1andrmtB. The genetic environment ofblaNDM-5showed a structure similar to those of pMC-NDM and pGUE-NDM, identified in Poland and France inE. coliof African and Indian origin, respectively.

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