Detection of quinolone-resistant Escherichia coli isolates belonging to clonal groups O25b:H4-B2-ST131 and O25b:H4-D-ST69 in raw sewage and river water in Barcelona, Spain

ABSTRACT The behavior outside the gut of seeded Escherichia coliO157:H7, naturally occurring E. coli, somatic coliphages, bacteriophages infecting O157:H7, and Shiga toxin 2 (Stx2)-encoding bacteriophages was studied to determine whether the last persist in the environment more successfully than their host bacteria. The ratios between the numbers of E. coli and those of the different bacteriophages were clearly lower in river water than in sewage of the area, whereas the ratios between the numbers of the different phages were similar. In addition, the numbers of bacteria decreased between 2 and 3 log units in in situ survival experiments performed in river water, whereas the numbers of phages decreased between 1 and 2 log units. Chlorination and pasteurization treatments that reduced by approximately 4 log units the numbers of bacteria reduced by less than 1 log unit the numbers of bacteriophages. Thus, it can be concluded that Stx2-encoding phages persist longer than their host bacteria in the water environment and are more resistant than their host bacteria to chlorination and heat treatment.

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Preliminary survey of antibiotic-resistant fecal indicator bacteria and pathogenic Escherichia coli from river-water samples collected in Oakland County, Michigan, 2003

Scientific Investigations Report ◽

10.3133/sir20055058 ◽

2005 ◽

Author(s):

Lisa R. Fogarty ◽

Joseph W. Duris ◽

Stephen S. Aichele

Keyword(s):

Escherichia Coli ◽

River Water ◽

Water Samples ◽

Fecal Indicator Bacteria ◽

Indicator Bacteria ◽

Preliminary Survey ◽

Fecal Indicator ◽

Antibiotic Resistant ◽

River Water Samples ◽

Oakland County

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Microbiological and Physicochemical Water Quality Assessments of River Water in an Industrial Region of the Northwest Coast of Borneo

Water ◽

10.3390/w10111648 ◽

2018 ◽

Vol 10 (11) ◽

pp. 1648 ◽

Cited By ~ 2

Author(s):

Sui Leong ◽

Johan Ismail ◽

Nurdiyana Denil ◽

Shahrul Sarbini ◽

Wafri Wasli ◽

...

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

River Water ◽

Oxygen Demand ◽

Northwest Coast ◽

Most Probable Number ◽

Probable Number ◽

Industrial Region ◽

Coliform Count ◽

The Mean

Urbanization and ever increasing socioeconomic activities have degraded natural resources globally. This study monitored water quality (WQI) based on physicochemical and microbial qualities of river water in an industrial region northwest coast of Borneo. Microbiological parameters tested included total viable count (TVC), coliform count (TC), faecal coliform count (FC), and Escherichia coli confirmation. Physicochemical constituents evaluated included water temperatures (T), pH, total dissolved solids (TDS), salinity, electrical conductivity (EC), dissolved oxygen (DO), biochemical oxygen demand (BOD), ammoniacal nitrogen (NH3-N), chemical oxygen demand (COD) and total suspended solids (TSS). The mean microbial counts of samples were: TVC (3.9 × 102–3.0 × 104 cfu mL−1), TC and FC (23–>1600 MPN 100 mL−1) (MPN: the most probable number) and Escherichia coli growth was confirmed. The mean values of pH, TDS, salinity, EC and BOD are significantly different (p < 0.05) between the rivers ranged: pH (5.05 ± 0.03–6.10 ± 0.10), TDS (0.0 ± 0.0–38,600 ± 50 mg L−1), salinity (0.01 ± 0.0–3.07 ± 0.07%), EC (650 ± 25–19,566.67 ± 251.66 µS cm−1), and BOD (0.23 ± 0.10–3.12 ± 0.02 mgO2 L−1). The tested parameters of samples exceeded international limits with the exception of COD and TSS. The WQI of river water tested ranged from 65–73 under class III. Most of the river water was slightly polluted and a potential threat to public health.

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The occurrence of antibiotic-resistant bacteria, including Escherichia coli, in municipal wastewater and river water

E3S Web of Conferences ◽

10.1051/e3sconf/201910000061 ◽

2019 ◽

Vol 100 ◽

pp. 00061 ◽

Cited By ~ 1

Author(s):

Adriana Osińska ◽

Ewa Korzeniewska ◽

Monika Harnisz ◽

Sebastian Niestępski ◽

Piotr Jachimowicz

Keyword(s):

Escherichia Coli ◽

Wastewater Treatment ◽

River Water ◽

Municipal Wastewater ◽

High Efficiency ◽

Treated Wastewater ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

E Coli

Wastewater treatment plants (WWTPs) are major reservoirs of antibiotic-resistant bacteria (ARB) which are transported to the natural environment with discharged effluents. Samples of untreated wastewater (UWW) and treated wastewater (TWW) from four municipal WWTPs and samples of river water collected upstream (URW) and downstream (DRW) from the effluent discharge point were analyzed in the study. The total counts of bacteria resistant to β-lactams and tetracyclines and the counts of antibiotic-resistant Escherichia coli were determined. Antibiotic-resistant bacteria, including antibiotic-resistant E. coli, were removed with up to 99.9% efficiency in the evaluated WWTPs. Despite the above, ARB counts in TWW samples were high at up to 1.25x105 CFU/mL in winter and 1.25x103 CFU/mL in summer. Antibiotic-resistant bacteria were also abundant (up to 103 CFU/ml) in URW and DRW samples collected in winter and summer. In both UWW and TWW samples, the counts of ARB and antibiotic-resistant E. coli were at least one order of magnitude lower in summer than in winter. The study revealed that despite the high efficiency of bacterial removal in the wastewater treatment processes, considerable amounts of ARB are released into the environment with TWW and that the percentage of ARB in total bacterial counts increases after wastewater treatment.

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