scholarly journals Identification of the novel spectinomycin resistance gene spw in methicillin-resistant and methicillin-susceptible Staphylococcus aureus of human and animal origin

2013 ◽  
Vol 68 (7) ◽  
pp. 1679-1680 ◽  
Author(s):  
S. Wendlandt ◽  
B. Li ◽  
C. Lozano ◽  
Z. Ma ◽  
C. Torres ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Gene ◽  
Animal Origin ◽  
The Novel ◽  
Methicillin Resistant ◽  
Spectinomycin Resistance

scholarly journals Identification of a Plasmid-Borne Resistance Gene Cluster Comprising the Resistance Genes erm(T), dfrK, and tet(L) in a Porcine Methicillin-Resistant Staphylococcus aureus ST398 Strain

2009 ◽  
Vol 54 (2) ◽  
pp. 915-918 ◽  
Author(s):  
Kristina Kadlec ◽  
Stefan Schwarz
Keyword(s):  
Staphylococcus Aureus ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Streptococcus Pyogenes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tetracycline Resistance ◽  
Sequence Type ◽  
The Novel ◽  
Methicillin Resistant ◽  
Resistance Gene Cluster

ABSTRACT A novel plasmid-borne resistance gene cluster comprising the genes erm(T) for macrolide-lincosamide-streptogramin B resistance, dfrK for trimethoprim resistance, and tet(L) for tetracycline resistance was identified in a porcine methicillin-resistant Staphylococcus aureus sequence type 398 (ST398) strain. This erm(T)-dfrK-tet(L) region was flanked by copies of the novel IS element ISSau10. The erm(T) region resembled that of Streptococcus pyogenes plasmid pRW35. The erm(T) gene of pKKS25 was expressed constitutively due to a 57-bp deletion in the erm(T) translational attenuator.

P900 Performance of the GeneXpert ®system for detection of methicillin-resistant Staphylococcus aureus including the non-typeable clone from animal origin

2007 ◽  
Vol 29 ◽  
pp. S233-S234
Author(s):  
M.W.M. Wassenberg ◽  
S. Nijssen ◽  
X.W. Huijsdens ◽  
A.M.C. Bergmans ◽  
C.M.J.E. Vandenbroucke-Grauls ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Animal Origin ◽  
Methicillin Resistant

scholarly journals Methicillin-Resistant Staphylococcus aureus in Commercial Swine Herds Is Associated with Disinfectant and Zinc Usage

2015 ◽  
Vol 81 (8) ◽  
pp. 2690-2695 ◽  
Author(s):  
Mackenzie Jonathan Slifierz ◽  
Robert M. Friendship ◽  
J. Scott Weese
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Resistance Gene ◽  
Methicillin Resistant ◽  
Content Type ◽  
Nursery Pigs ◽  
Herd Level ◽  
Antimicrobial Resistance Genes ◽  
Level Information ◽  
Swine Herds

ABSTRACTMethicillin-resistantStaphylococcus aureus(MRSA) originating from swine is concerning for public health, but an understanding of the emergence and persistence of MRSA in nursery herds is lacking. The aim of this study was to determine whether MRSA in nursery pigs is associated with particular herd-level parameters, including the use of antimicrobials, disinfectants, and heavy metals, which may be driving the selection and persistence of antimicrobial resistance. Nasal cultures for MRSA were completed for 390 pigs from 26 farms at the end of the suckling phase and again at 3 weeks postweaning. Herd-level information was collected, and a random subset of MRSA isolates was screened for resistance to zinc and quaternary ammonium compounds (QACs). Multivariate analysis revealed that in-feed concentrations of zinc (P< 0.001) and frequent disinfection of nursery pens (P< 0.001) are associated with MRSA shedding in nursery pigs. Furthermore, 62.5% (25/40) of MRSA isolates carried the zinc resistance geneczrCand demonstrated decreased susceptibility to zinc. All MRSA isolates carried at least 1 QAC resistance gene. The most common genotype wasqacG qacH smr, which occurred in 32.5% (13/40) of isolates. Seven isolates (17.5%) demonstrated a significant tolerance to benzalkonium chloride, indicating a potential to survive commercial QAC exposure in the presence of organic matter. Overall, these findings indicate that high levels of in-feed zinc and QAC-based disinfectants are important drivers in the selection and persistence of MRSA in commercial swine herds, and these agents may be coselecting for other antimicrobial resistance genes.

Molecular Characterization and Antimicrobial Resistance Profile of Methicillin-Resistant Staphylococcus aureus in Retail Chicken

2015 ◽  
Vol 78 (10) ◽  
pp. 1879-1884 ◽  
Author(s):  
KHALID IBRAHIM SALLAM ◽  
SAMIR MOHAMMED ABD-ELGHANY ◽  
MOHAMED ELHADIDY ◽  
TOMOHIRO TAMURA
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Contamination Rate ◽  
Methicillin Resistant ◽  
Food Of Animal Origin

The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is of increasing interest, raising questions about the presence of MRSA in food of animal origin and potential sources of transmission to humans via the food chain. In this study, the prevalence, molecular characterization, virulence factors, and antimicrobial susceptibility patterns of MRSA isolates from 200 retail raw chicken samples in Egypt were determined. MRSA was detected by positive amplification of the mecA gene in 38% (76 of 200) of chicken samples analyzed. This represents a potential public health threat in Egypt, as this contamination rate seems to be the highest among other studies reported worldwide. Furthermore, genes encoding α-hemolysin (hla) and staphylococcal enterotoxins (sea, seb, and sec) were detected in all of the 288 MRSA isolates. Nonetheless, none of the strains tested carried tst, the gene encoding toxic shock syndrome toxin 1. Antimicrobial resistance of MRSA isolates was most frequently detected against penicillin (93.4%), ampicillin (88.9%), and cloxacillin (83.3%). These results suggest that retail chicken might be a significant potential source for transmission of multidrug-resistant and toxigenic S. aureus in Egypt. This underlines the need for stricter hygienic measures in chicken production in Egypt to minimize the risk of transmission of these strains to consumers. To the best of our knowledge, this is the first study that reports the isolation and molecular characterization of MRSA in retail chicken samples in Egypt.

scholarly journals New Macrolide-Lincosamide-Streptogramin B Resistance Gene erm(48) on the Novel Plasmid pJW2311 in Staphylococcus xylosus

2017 ◽  
Vol 61 (7) ◽  
Author(s):  
Juliette R. K. Wipf ◽  
Matthew C. Riley ◽  
Stephen A. Kania ◽  
David A. Bemis ◽  
Sabrina Andreis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Gene ◽  
Genome Sequencing ◽  
Bovine Mastitis ◽  
Cloning And Expression ◽  
Whole Genome ◽  
The Novel ◽  
Content Type ◽  
Staphylococcus Xylosus ◽  
Macrolide Resistance Gene

ABSTRACT Whole-genome sequencing of Staphylococcus xylosus strain JW2311 from bovine mastitis milk identified the novel 49.3-kb macrolide-lincosamide-streptogramin B (MLSB) resistance plasmid pJW2311. It contained the macrolide resistance gene mph(C), the macrolide-streptogramin B resistance gene msr(A), and the new MLSB resistance gene erm(48) and could be transformed into Staphylococcus aureus by electroporation. Functionality of erm(48) was demonstrated by cloning and expression in S. aureus.

scholarly journals An IS257-Derived Hybrid Promoter Directs Transcription of a tetA(K) Tetracycline Resistance Gene in theStaphylococcus aureus Chromosomal mecRegion

2000 ◽  
Vol 182 (12) ◽  
pp. 3345-3352 ◽  
Author(s):  
Alice E. Simpson ◽  
Ronald A. Skurray ◽  
Neville Firth
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Gene ◽  
Gene Dosage ◽  
Tetracycline Resistance ◽  
Resistance Determinant ◽  
Tetracycline Resistance Gene ◽  
Methicillin Resistant ◽  
Hybrid Promoter ◽  
Genetic Rearrangements

ABSTRACT Transcription of the tetA(K) tetracycline resistance determinant encoded by an IS257-flanked cointegrated copy of a pT181-like plasmid, located within the chromosomal mecregion of a methicillin-resistant Staphylococcus aureusisolate, has been investigated. The results demonstrated that transcription of tetA(K) in this strain is directed by both an IS257-derived hybrid promoter, which is stronger than the native tetA(K) promoter in the autonomous form of pT181, and a complete outwardly directed promoter identified within one end of IS257. Despite lower gene dosage, the chromosomal configuration was shown to afford a higher level of resistance than that mediated by pT181 in an autonomous multicopy state. Furthermore, competition studies revealed that a strain carrying the chromosomaltetA(K) determinant exhibited a higher level of fitness in the presence of tetracycline but not in its absence. This finding suggests that tetracycline has been a selective factor in the emergence of strains carrying a cointegrated pT181-like plasmid in their chromosomes. The results highlight the potential of IS257to influence the expression of neighboring genes, a property likely to enhance its capacity to mediate advantageous genetic rearrangements.

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