Fluorometric Determination of Chlortetracycline in Low Level Mixed Feeds

1964 ◽  
Vol 47 (6) ◽  
pp. 1157-1161
Author(s):  
Stanley E Katz ◽  
Joseph Spock
Keyword(s):  
Ion Exchange ◽  
Sodium Carbonate ◽  
Fluorometric Determination ◽  
Aqueous Sodium ◽  
Exchange Column ◽  
Aqueous Sodium Carbonate ◽  
Ion Exchange Column ◽  
Low Levels ◽  
Mixed Feeds

Abstract The fluorometric determination of low levels of chlortetracycline in mixed feeds is based upon the separation of the chlortetracycline on a Decalso ion exchange column after extraction from the feed. The chlortetracycline is eluted from the resin after conversion to isochlortetracycline by hot aqueous sodium carbonate. The isochlortetracycline is measured fluorometrically. Results agree with microbial assays.

Fluorometric Determination of Chlortetracycline in Mixed Feeds

1964 ◽  
Vol 47 (2) ◽  
pp. 203-208
Author(s):  
Stanley E Katz ◽  
Joseph Spock
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ammonium Hydroxide ◽  
Solvent System ◽  
Ion Exchange Resin ◽  
Alkaline Degradation ◽  
Fluorometric Determination ◽  
Mixed Feeds ◽  
Microbiological Assays

Abstract The fluorometric procedure for chlortetracycline in mixed feeds is based upon the degradation of chlortetracycline in alkaline solution to isochlortetracycline. The fluorescence of the isochlortetracycline is directly proportional to the concentration of chlortetracycline present prior to alkaline degradation. Chlortetracycline is extracted from mixed feeds with an acidmethanol solvent system, separated from the feed extract by adsorption on a Dowex 50 ion exchange resin, and eluted from the resin by ammonium hydroxide after conversion to isochlortetracycline. The isochlortetracycline is measured fluorometrically. Recoveries from known feeds are generally greater than 90% and agreements with microbiological assays are very close.

A Single Column Method for the Determination of Urinary δ-Aminolevulinic Acid

1969 ◽  
Vol 15 (3) ◽  
pp. 183-189 ◽  
Author(s):  
Mu-Wan Sun ◽  
Edward Stein ◽  
Frances W Gruen
Keyword(s):  
Ion Exchange ◽  
Lead Poisoning ◽  
Aminolevulinic Acid ◽  
Simple Method ◽  
Exchange Column ◽  
Column Method ◽  
Single Column ◽  
Ion Exchange Column

Abstract A simple method for determination of urinary δ-aminolevulinic acid is described. Its advantage over procedures now in use is in the utilization of a single ion-exchange column instead of two ion-exchange columns, with resulting saving of time, labor, and material. The method is accurate and useful in screening for lead poisoning.

Fast, Short-Column Separation and Fluoromeitric Determination of Monosodium Glutamate in Foods

1975 ◽  
Vol 58 (1) ◽  
pp. 58-61 ◽  
Author(s):  
Elia D Coppola ◽  
Spence N Christie ◽  
J Gordon Coppola
Keyword(s):  
Standard Deviation ◽  
Ion Exchange ◽  
Monosodium Glutamate ◽  
Official Method ◽  
Average Recovery ◽  
Exchange Column ◽  
Column Separation ◽  
Ion Exchange Column ◽  
Fast Procedure

Abstract In a simple and fast procedure, monosodium glutamate in food products is first separated by a short ion exchange column and subsequently determined fluorometrically with fluorescamine. An average recovery of 90.8% with a standard deviation of ±6.67% was obtained for added monosodium glutamate in a series of 8 products. As little as 0.05% monosodium glutamate can be determined. The method is faster than the official method and saves 5 hr/ determination.

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