A Single Column Method for the Determination of Urinary δ-Aminolevulinic Acid

1969 ◽  
Vol 15 (3) ◽  
pp. 183-189 ◽  
Author(s):  
Mu-Wan Sun ◽  
Edward Stein ◽  
Frances W Gruen
Keyword(s):  
Ion Exchange ◽  
Lead Poisoning ◽  
Aminolevulinic Acid ◽  
Simple Method ◽  
Exchange Column ◽  
Column Method ◽  
Single Column ◽  
Ion Exchange Column

Abstract A simple method for determination of urinary δ-aminolevulinic acid is described. Its advantage over procedures now in use is in the utilization of a single ion-exchange column instead of two ion-exchange columns, with resulting saving of time, labor, and material. The method is accurate and useful in screening for lead poisoning.

Simple Method for Determination of Urinary δ-Aminolevulinic Acid as an Index of Lead Exposure

1972 ◽  
Vol 18 (12) ◽  
pp. 1534-1536 ◽  
Author(s):  
Katsumaro Tomokuni ◽  
Masana Ogata
Keyword(s):  
Aqueous Solution ◽  
Quantitative Analysis ◽  
Ion Exchange ◽  
Ethyl Acetate ◽  
Lead Exposure ◽  
Ethyl Acetoacetate ◽  
Aminolevulinic Acid ◽  
Simple Method ◽  
Ion Exchange Column

Abstract A simple method is described for the quantitative analysis of urinary δ-aminolevulinic acid. 2-Methyl3-carbethoxy-4-(3-propionic acid) pyrrole, produced by the condensation of δ-aminolevulinic acid with ethyl acetoacetate, is almost completely extracted from an aqueous solution with ethyl acetate without resorting to ion-exchange column chromatography. The pyrrole is determined colorimetrically by treating an aliquot of the extract with a modified Ehrlich’s reagent.

New Method for Determination of Aminolaevulinate Dehydratase Activity of Human Erythrocytes as an Index of Lead Exposure

1974 ◽  
Vol 20 (10) ◽  
pp. 1287-1291 ◽  
Author(s):  
Katsumaro Tomokuni
Keyword(s):  
Enzyme Activity ◽  
Ion Exchange ◽  
Present Method ◽  
Aminolevulinic Acid ◽  
Usual Method ◽  
Human Erythrocytes ◽  
New Method ◽  
Ion Exchange Column ◽  
Dehydratase Activity

Abstract I describe a new method for measurement of aminolaevulinate dehydratase (EC 4.2.1.24) activity of human erythrocytes. In this method, the amount of substrate δ-aminolevulinic acid consumed (instead of the amount of porphobilinogen formed) is determined colorimetrically. In the incubation mixture, the δ-aminolevulinic acidpyrrole produced by the condensation of δ-aminolevulinic acid with ethyl acetoacetate is separated from porphobilinogen by extraction with ethyl acetate, without resorting to ion-exchange column chromatography. The pyrrole-containing extract is treated with a modified Ehrlich's reagent. Activity of the enzyme is expressed as micromoles of δ-aminolevulinic acid consumed per minute per liter of erythrocytes. Enzyme activity is more accurately estimated by the present method than by the usual method in which porphobilinogen is measured.

Fluorometric Determination of Chlortetracycline in Low Level Mixed Feeds

1964 ◽  
Vol 47 (6) ◽  
pp. 1157-1161
Author(s):  
Stanley E Katz ◽  
Joseph Spock
Keyword(s):  
Ion Exchange ◽  
Sodium Carbonate ◽  
Fluorometric Determination ◽  
Aqueous Sodium ◽  
Exchange Column ◽  
Aqueous Sodium Carbonate ◽  
Ion Exchange Column ◽  
Low Levels ◽  
Mixed Feeds

Abstract The fluorometric determination of low levels of chlortetracycline in mixed feeds is based upon the separation of the chlortetracycline on a Decalso ion exchange column after extraction from the feed. The chlortetracycline is eluted from the resin after conversion to isochlortetracycline by hot aqueous sodium carbonate. The isochlortetracycline is measured fluorometrically. Results agree with microbial assays.

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