Microbiological Assay of Low Chlortetracycline Concentrations in Animal Feeds

1965 ◽  
Vol 48 (2) ◽  
pp. 271-277
Author(s):  
Anthony Abbey ◽  
David B Hewel
Keyword(s):  
Sodium Hypochlorite ◽  
Animal Feed ◽  
Microbiological Assay ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution ◽  
Animal Feeds ◽  
Low Levels

Abstract Satisfactory microbiological values were obtained for animal feeds containing low levels of cblortetracycline (CTC), 5.0–40g CTC/ton. Results were comparable when calculated against both compensating CTC standard curves prepared from blank feed extracts and laboratory-inactivated CTC feed extracts. The inactivation of CTC in animal feed extracts by 5.25% sodium hypochlorite solution and heat was investigated, and it appeared to offer a convenient alternative for preparing compensating CTC standard curves.

Collaborative Study of Microbiological Assay for Low Chlortetracycline Concentrations in Final Feeds

1967 ◽  
Vol 50 (2) ◽  
pp. 446-449
Author(s):  
Anthony Abbey
Keyword(s):  
Sodium Hypochlorite ◽  
Animal Feed ◽  
Collaborative Study ◽  
Microbiological Assay ◽  
Poultry Feed ◽  
Assay Procedure ◽  
Coefficients Of Variation ◽  
Commercial Poultry ◽  
Low Levels ◽  
The Individual

Abstract Two batches of commercial poultry feed containing low levels of chlortetracycline (CTC) activity (ca 10 ppm) were assayed collaboratively in 15 laboratories. The coefficients of variation for the individual batches were 10.0 and 9.2%, respectively; combining the data for both batches gave a coefficient of variation of 9.6%. The microbiological assay procedure, utilizing compensating CTC standard curves prepared with a diluent containing sodium hypochlorite- inactivated animal feed extracts, was found applicable in all 15 participating laboratories. The method is recommended for adoption as official, first action.

Collaborative Test of a Microbiological Assay for Low Chlortetracycline Concentrations In Final Feeds

1966 ◽  
Vol 49 (3) ◽  
pp. 483-488
Author(s):  
Anthony Abbey
Keyword(s):  
Sodium Hypochlorite ◽  
Coefficient Of Variation ◽  
Animal Feed ◽  
Microbiological Assay ◽  
Collaborative Test ◽  
Coefficients Of Variation ◽  
Commercial Poultry ◽  
Low Levels ◽  
Cattle Feeds ◽  
Combined Data

Abstract Commercial poultry, cattle, and swine feeds, containing low levels of chlortetracycline (CTC) activity (5, 10, and 20 ppm CTC), were assayed collaboratively in eight laboratories by compensating CTC standard curves prepared with diluent from sodium hypochlorite-inactivated animal feed extracts. The coefficients of variation for poultry, cattle, and swine feeds, individually, were 20, 13, and 10%, respectively. The combined data from those laboratories on both poultry and cattle feeds yielded a coefficient of variation of 12%, while the combined data from those laboratories assaying poultry and swine feeds gave a coefficient of variation of 15%. The inactivation of CTC in animal feed extracts by sodium hypochlorite plus heat appears to be operative in all eight laboratories.

scholarly journals Safety assessment of an etidronate in a sodium hypochlorite solution: randomized double‐blind trial

2019 ◽  
Vol 52 (9) ◽  
pp. 1274-1282 ◽  
Author(s):  
N. V. Ballal ◽  
P. Gandhi ◽  
P. A. Shenoy ◽  
V. Shenoy Belle ◽  
V. Bhat ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Safety Assessment ◽  
Sodium Hypochlorite Solution ◽  
Double Blind Trial ◽  
Double Blind ◽  
Hypochlorite Solution ◽  
Blind Trial

Reduction of Microbial Pathogens during Apple Cider Production Using Sodium Hypochlorite, Copper Ion, and Sonication

2004 ◽  
Vol 67 (4) ◽  
pp. 766-771 ◽  
Author(s):  
STEPHANIE L. RODGERS ◽  
ELLIOT T. RYSER
Keyword(s):  
Sodium Hypochlorite ◽  
Copper Ion ◽  
Microbial Pathogens ◽  
Sodium Hypochlorite Solution ◽  
Escherichia Coli O157 ◽  
Hypochlorite Solution ◽  
Apple Cider ◽  
E Coli ◽  
Log Reduction ◽  
Pathogen Reduction

Sodium hypochlorite (100 ppm), copper ion water (1 ppm), and sonication (22 to 44 kHz and 44 to 48 kHz) were assessed individually and in combination for their ability to reduce populations of Escherichia coli O157:H7 and Listeria monocytogenes on apples and in apple cider. Commercial unpasteurized cider was inoculated to contain approximately 106 CFU/ml of either pathogen and then sonicated at 44 to 48 kHz, with aliquots removed at intervals of 30 to 60 s for up to 5 min and plated to determine numbers of survivors. Subsequently, whole apples were inoculated by dipping to contain approximately 106 CFU/g E. coli O157:H7 or L. monocytogenes, held overnight, and then submerged in 1 ppm copper ion water with or without 100 ppm sodium hypochlorite for 3 min with or without sonication at 22 to 44 kHz and examined for survivors. Treated apples were also juiced, with the resulting cider sonicated for 3 min. Populations of both pathogens decreased 1 to 2 log CFU/ml in inoculated cider following 3 min of sonication. Copper ion water alone did not significantly reduce populations of either pathogen on inoculated apples. However, when used in combination with sodium hypochlorite, pathogen levels decreased approximately 2.3 log CFU/g on apples. Sonication of this copper ion–sodium hypochlorite solution at 22 to 44 kHz did not further improve pathogen reduction on apples. Numbers of either pathogen in the juice fraction were approximately 1.2 log CFU/ml lower after being juiced, with sonication (44 to 48 kHz) of the expressed juice decreasing L. monocytogenes and E. coli O157:H7 populations an additional 2 log. Hence, a 5-log reduction was achievable for both pathogens with the use of copper ion water in combination with sodium hypochlorite followed by juicing and sonication at 44 to 48 kHz.

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