Detection of Admixtures of Turmeric, Curcuma longa Linn., with Curcuma aromatica Salisb. by Thin Layer and Gas-Liquid Chromatography

1979 ◽  
Vol 62 (6) ◽  
pp. 1333-1337
Author(s):  
Kalyan G Raghuveer ◽  
Venkatesa S Govindarajan
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Sulfuric Acid ◽  
Thin Layer ◽  
Curcuma Longa ◽  
Volatile Oil ◽  
Gas Liquid Chromatography ◽  
Chromatographic Methods ◽  
Hexane Extracts

Abstract Simple and definitive thin layer chromatographic methods are described for the detection of admixtures of Curcuma longa with Curcuma aromatica at the 5% levels. The tests are performed on hexane extracts, thus avoiding distillation of the volatile oil, and are based on the separation of high boiling sesquiterpene compounds by hexane or benzene. Chromatograms are sprayed with vanillin-sulfuric acid or Gibbs reagent to give distinct spots for C. aromatica which are absent from C. longa. Gas chromatography of the extracts also distinguishes the admixtures through a late-eluting peak for C. aromatica.

Actinopolyspora halophila does not contain mycolic acids

1987 ◽  
Vol 33 (9) ◽  
pp. 822-823 ◽  
Author(s):  
M. Kates ◽  
Suzanne Porter ◽  
D. J. Kushner
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Gas Liquid Chromatography ◽  
Pyrolysis Gas ◽  
Mycolic Acids ◽  
Pyrolysis Gas Chromatography ◽  
Halophilic Actinomycete ◽  
Layer Chromatography

Studies using the techniques of thin-layer chromatography, mass spectrometry, gas–liquid chromatography, and pyrolysis gas chromatography have shown conclusively that the extreme halophilic actinomycete Actinopolyspora halophila does not contain any detectable mycolic acids. This finding is consistent with the grouping of A. halophila with other wall chemotype IV actinomycetes.

Radioimmunoassay of methaqualone in human urine compared with chromatographic methods.

1978 ◽  
Vol 24 (9) ◽  
pp. 1473-1476 ◽  
Author(s):  
S J Mulé ◽  
M Kogan ◽  
D Jukofsky
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Human Urine ◽  
Reference Method ◽  
Cross Reactivity ◽  
Complete Agreement ◽  
Gas Liquid Chromatography ◽  
Reliable Technique ◽  
Chromatographic Methods ◽  
Liquid Chromatographic

Abstract The 125I-radioimmunoassay for methaqualone in human urine was evaluated by a comparison with newly modified gas-liquid chromatographic and thin-layer chromatographic methods. The statistically significant sensitivity value for the radioimmunoassay was at 2 microgram of methaqualone per liter of urine. The coefficient of variation was 2.88 +/- 0.39% interassay and 2.71 +/- 0.16% intraassay. There was cross-reactivity only with metabolites of methaqualone, 4'-hydroxymethaqualone being twice as sensitively measured as methaqualone. There was complete agreement between results by radioimmunoassay and by gas-liquid chromatography in 96.7% of the samples analyzed. Only 1.2% of the radioimmunoassay values were false positives, and 2.1% false negatives (phi = 0.8917, P less than 0.001). Comparisons between the thin-layer chromatographic data and the gas-liquid chromatographic or radioimmunoassay data showed less agreement because of the 50- to 200-fold higher sensitivity of the latter two techniques. Gas-liquid chromatography therefore appears to represent the best reference method for the evaluation of the radioimmunoassay, which appears to be a very sensitive and reliable technique for detecting methaqualone and its metabolites in human urine.

Analysis of sugars in dwarf mistletoe nectar

1974 ◽  
Vol 52 (12) ◽  
pp. 2533-2538 ◽  
Author(s):  
J. W. Brewer ◽  
K. J. Collyard ◽  
C. E. Lott Jr.
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Sugar Content ◽  
Gas Liquid Chromatography ◽  
Dwarf Mistletoe ◽  
Total Solids ◽  
High Sugar ◽  
Insect Pollinator ◽  
High Sugar Content

Nectar produced by pistillate flowers of the dwarf mistletoe Arceuthobium abietinum Engelm. was analyzed by thin-layer and gas–liquid chromatography. The inconspicuous (0.5–1.5 mm) flowers produce relatively large (0.24 μliter) droplets of highly concentrated nectar (58–92% total solids, expressed as sugars). The major components were glucose (48%), fructose (39%), and sucrose (11%). Traces of other sugars were also present. It is suggested that nectar of this high sugar content is probably a strong insect pollinator attractant, and this, in turn, may be a major factor in the successful spread of the parasitic dwarf mistletoes.

DETERMINATION OF PLASMA OESTRIOL IN NORMAL PREGNANCY AND LABOUR USING GAS-LIQUID CHROMATOGRAPHY

1971 ◽  
Vol 51 (3) ◽  
pp. 447-454 ◽  
Author(s):  
W. COOPER ◽  
M. G. COYLE ◽  
J. A. MILLS
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Internal Standard ◽  
Normal Pregnancy ◽  
Gas Liquid Chromatography ◽  
Chemical Purification ◽  
Peripheral Plasma ◽  
Pregnancy And Delivery

SUMMARY A method is described for estimating oestriol in 2–10 ml samples of human pregnancy peripheral plasma. It incorporates acid hydrolysis, chemical purification, methylation, chromatography on alumina columns, formation of a derivative and quantitative determination by gas chromatography. A radioactive internal standard was added to correct for procedural losses. Plasma oestriol determinations in five normal patients throughout pregnancy and delivery are reported.

ANALYSIS OF CHLORORGANIC COMPOUNDS IN FODDER FOR BROILERCHICKENS BY THE METHOD OF GAS-LIQUID CHROMATOGRAPHY WITH MASSSPECTROMETER DETECTOR

2020 ◽  
Vol 1 (2) ◽  
pp. 205-208
Author(s):  
A.A. Parshutina ◽  
◽  
A.A. Solovyova ◽  
L.P. Satyukova ◽  
E.G. Shubina ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Organochlorine Pesticides ◽  
Mass Spectrometer ◽  
Broiler Chickens ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography

The article shows the importance of the study of feed for broiler chickens on the content of organochlorine pesticides. These substances in significant concentrations can disrupt the development of birds and cause mass poisoning. The study of formula feed for broiler chickens «prestart» and «start» by two methodswas conducted: a certified method for the determination of organochlorine pesticides in feed and formula feed (thin layer chromatography) and a method for detecting pesticides not certified for feed (gas-liquid chromatography with mass spectrometer detector). During the experiment, the presence of organochlorine pesticides in several formulafeed samples was revealed.

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