Recommended Modification of Dilution Procedure Used for Bacteriological Examination of Shellfish

1984 ◽  
Vol 67 (1) ◽  
pp. 197-198
Author(s):  
David W Cook ◽  
Gerard S Pabst
Keyword(s):  
Public Health ◽  
Fecal Coliform ◽  
Coliform Bacteria ◽  
Bacteriological Examination ◽  
American Public Health Association ◽  
Bacteriological Analysis ◽  
Fecal Coliform Bacteria ◽  
Health Association ◽  
Number Of Bacteria

Abstract The dilution procedure recommended by the American Public Health Association for use in bacteriological analysis of shellfish samples may cause an underestimation of the number of bacteria in shellfish. Modifications of that procedure to include preparation of the first dilution of the shellfish homogenate on a weight rather than a volume basis, and the inoculation of 1 g portions of the shellfish from a dilution of the homogenate, have been shown to significantly increase the recovery of fecal coliform bacteria from oyster samples. It is recommended that these modifications be adopted by the American Public Health Association.

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

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