Identification and Semiquantitation of Monensin Sodium in Liver Tissue

1984 ◽  
Vol 67 (4) ◽  
pp. 845-846
Author(s):  
Elizabeth E Martinez ◽  
Wilbert Shimoda
Keyword(s):  
Liver Tissue ◽  
Animal Feed ◽  
Beef Liver ◽  
Zones Of Inhibition ◽  
Liver Tissues

Abstract A bioautographic technique for the determination of monensin sodium in animal feed has been applied to chicken and beef liver tissues. The reliable limit of sensitivity is 100 ppb, but 10 ppb can be detected. This technique can be used to semiquantitate monensin in tissues by comparing the zones of inhibition of unknown test samples against monensin standards.

An anion induced multisignaling probe for Hg2+ and its application for fish kidney and liver tissue imaging studies

2015 ◽  
Vol 44 (29) ◽  
pp. 13186-13195 ◽  
Author(s):  
Sandip Mandal ◽  
Arnab Banerjee ◽  
Debasree Ghosh ◽  
Dipak Kumar Mandal ◽  
Damir A. Safin ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Fluorescence Method ◽  
Tissue Imaging ◽  
Selective Recognition ◽  
Imaging Studies ◽  
Highly Efficient ◽  
Fish Kidney ◽  
Liver Tissues

A highly efficient binder for the selective recognition and determination of Hg(NO3)2 concentration in kidney and liver tissues of fish using a fluorescence method is described.

Determination of Monensin Sodium Residues in Beef Liver Tissue by Liquid Chromatography of a Fluorescent Derivative

1985 ◽  
Vol 68 (6) ◽  
pp. 1149-1153 ◽  
Author(s):  
Elizabeth E Martinez ◽  
Wilbert Shimoda
Keyword(s):  
Liquid Chromatography ◽  
Liver Tissue ◽  
Uv Absorbance ◽  
Uv Detector ◽  
Beef Liver ◽  
Fluorescent Derivative

Abstract Monensin sodium does not have an ultraviolet (UV) absorbance above 220 nm, and therefore cannot be detected by liquid chromatography (LC) with a UV detector. A method was developed in which monensin residues are extracted from beef liver tissue, acetylated, partitioned, and reacted with 9-anthryldiazomethane to form a fluorescent derivative for quantitation by LC. The reliable level of sensitivity is 50 ppb, but 15 pjib can be detected. Recoveries ranged between 71 and 96% with an average of 83.5%.

Liquid Chromatographic Determination of Multiresidue Fluorescent Derivatives of Lonophore 1 Compounds, Monensin, Salinomycin, Narasin, and Lasalocid, in Beef Liver Tissue

1986 ◽  
Vol 69 (4) ◽  
pp. 637-641 ◽  
Author(s):  
Elizabeth E Martinez ◽  
Wilbert Shimoda
Keyword(s):  
Liver Tissue ◽  
Chromatographic Determination ◽  
Fluorometric Detection ◽  
Fluorescence Detector ◽  
Beef Liver ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Layer Chromatography ◽  
Derivatives Of

Abstract A liquid chromatographic (LC) method with fluorometric detection was developed to quantitatively determine residue levels of monensin, salinomycin, narasin, and lasalocid in beef liver tissue. The ionophores are extracted from the tissue, purified by both alumina and Sephadex LH-20 column chromatography, and then derivatized. Lasalocid was directly esterified with 9-anthryIdiazomethane (ADAM), but monensin, salinomycin, and narasin were first acetylated with acetic anhydride and then esterified with ADAM. The ADAM derivatives were purified on a silica gel column and separated by LC using an RP C-8 5 μm column. A fluorescence detector set at 365 nm (excitation) and 418 nm (emission) was used to monitor the column effluent. The detection limits were 0.15 ppm, and the calibration curves were linear between 0.5 and 5.0 ppm for all 4 ionophores. Mean recoveries were 57, 70, 75, and 90% for lasalocid (5 ppm), monensin (2.5 ppm), salinomycin (2.5 ppm), and narasin (2.5 ppm), respectively. The ionophores were also separated and semiquantitated by using bioautography and thin layer chromatography with a vanillin spray.

scholarly journals Determination of optimum animal feed raw material order allocation

2021 ◽  
Vol 713 (1) ◽  
pp. 012001
Author(s):  
D Wahyuni ◽  
M T Sembiring ◽  
I Budiman ◽  
T Utari ◽  
C D N Silaen
Keyword(s):  
Animal Feed ◽  
Raw Material ◽  
Order Allocation

scholarly journals Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

2014 ◽  
Vol 43 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Cíntia Lima Gouveia ◽  
Isabelle Cristine Melo Freire ◽  
Maria Luísa de Alencar e Silva Leite ◽  
Rebeca Dantas Alves Figueiredo ◽  
Leopoldina de Fátima Dantas de Almeida ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Sodium Bicarbonate ◽  
Fungal Growth ◽  
Antifungal Effect ◽  
Dental Prostheses ◽  
Diffusion Technique ◽  
Zones Of Inhibition ◽  
And Control

Introduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs(r) (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% - Periogard (r) (S5), Mouthrinse based on essential oils - Listerine(r) (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 106 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growth less than 2×104 CFU/mL and an accentuation of the microbial death curve were observed after 30 minutes, with exception for S3 and control groups. Conclusion: The studied compounds, with the exception of Sodium Bicarbonate, have antifungal effect against C. albicans, which contribute for dental prostheses hygiene.

scholarly journals The penetration of methanol into bovine cardiac and hepatic tissues is faster than ethanol and formalin

2018 ◽  
Author(s):  
Michelle Steicke ◽  
Guang Yang ◽  
Tam Nguyen Dinh ◽  
Matthew Dunster-Jones ◽  
Owen Sargisson ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Penetration Rate ◽  
Biological Tissues ◽  
Heart Tissue ◽  
Penetration Distance ◽  
Before And After ◽  
Tissue Shrinkage ◽  
The Mean ◽  
Post Hoc ◽  
Liver Tissues

Methanol, ethanol and formalin are commonly used as fixatives to preserve biological tissues from decay in the preparation of histological sections. Fixation of the inner layers of the tissue depends on the ability of the fixative to diffuse into the tissue. It is unknown whether methanol penetrates tissues at similar rates to other fixatives. This study aimed to compare the penetration rates of methanol, ethanol and formalin into bovine heart and liver tissues. The penetration distance and tissue shrinkage or expansion were measured by analysing the digital images of tissue before and after immersion in different fixatives for 1, 2, 6 or 10 h. Data were analysed using two-way ANOVA, followed by Bonferroni’s post-hoc test. The penetration distance of methanol was significantly greater in both heart and liver tissues compared with that of ethanol (N=4, P<0.001). Methanol or ethanol immersion led to similar shrinkage of both tissues (P>0.05). The penetration rate of formalin was similar to that of ethanol in both tissues however it was significantly slower than methanol (N=4, P<0.005 in the heart; P<0.001 in the liver). The mean penetration coefficients of methanol, formalin and ethanol in the heart tissue were 2.609, 1.994 and 1.801, respectively, and 3.012, 2.153 and 2.113, respectively, in the liver tissue. The penetration coefficient of methanol was significantly greater than that of ethanol or formalin in both tissues (P<0.001 for each comparison). In conclusion, methanol penetrates tissue significantly faster than ethanol and formalin.

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