Evaluation of Asparagine in the AOAC Use-Dilution Method

1986 ◽  
Vol 69 (6) ◽  
pp. 1003-1005
Author(s):  
Eugene C Cole ◽  
William A Rutala
Keyword(s):  
Stainless Steel ◽  
Bacterial Adherence ◽  
Dilution Method ◽  
And Storage

Abstract An asparagine solution (0.1%) is required in the AOAC use-dilution method to cover stainless steel carriers during sterilization and storage. The rationale for using an asparagine soak is unclear. It has been proposed that asparagine enhances bacterial adherence to carriers or inhibits corrosion of the metal carriers or both. The former theory was assessed in this study by comparing bacterial adherence on seeded carriers stored in 0.1 and 1% asparagine solution to those stored dry or in water. The anticorrosive properties of asparagine on stainless steel carriers were microscopically evaluated after 9 months. The results demonstrate that asparagine neither enhances bacterial adherence to carriers nor is necessary to prevent corrosion.

Surface conditioning of stainless steel coupons with skim milk, buttermilk, and butter serum solutions and its effect on bacterial adherence

Food Control ◽  
2014 ◽  
Vol 42 ◽  
pp. 94-100 ◽  
Author(s):  
Nguyen Manh Dat ◽  
Le Duc Manh ◽  
Daisuke Hamanaka ◽  
Duong Van Hung ◽  
Fumihiko Tanaka ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Skim Milk ◽  
Bacterial Adherence ◽  
Surface Conditioning

scholarly journals The influence of ethanol extracts concentration of mengkudu fruit (Morinda citrifolia) and storage time toward beef quality

2011 ◽  
Vol 9 (1) ◽  
pp. 26-32
Author(s):  
AVE SONIA RAHMAN ◽  
TJAHJADI PURWOKO ◽  
ESTU RETNANINGTYAS NUGRAHENI
Keyword(s):  
Storage Time ◽  
Ph Value ◽  
Morinda Citrifolia ◽  
Plate Count ◽  
Dilution Method ◽  
Beef Quality ◽  
Fresh Meat ◽  
Randomized Design ◽  
Ethanol Extracts ◽  
And Storage

Rahman AS, Purwoko T, Nugraheni ER. 2011. The influence of ethanol extracts concentration of mengkudu fruit (Morinda citrifolia) and storage time toward beef quality. Biofarmasi 9: 26-32. The high protein content of beef makes it easy to get a contamination caused by an activity of bacteria. The use of formalin as a preservative substance especially for beef can make a damage to health, so it is required to find preservative substances as alternative to substitute formalin. Mengkudu (Morinda citrifolia L.) has antibacteria compounds to the decompose bacteria of beef, so mengkudu fruit can be one of natural preservative substance alternatives, especially for beef. The purposes of this research were to determine the influence of ethanol extracts concentration of mengkudu fruit and a storage-time effect toward bacteria amount, and to determine the concentration that had a same activity with formalin to combate the replication of bacteria. This research used two-way completely randomized design. The samples were fresh meat beef that be submerged in mengkudu extract with concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% and formalin 1% as a comparator. After that, the beef was storaged in a room temperature during 16 hours, and every 4 hours it was tested for water content, pH value, beef temperature and a total plate count (TPC) test with a dilution method, and then the number of bacteria colonies with the limit of the bacteria amount of the beef to consume might not be more than 1x104 CFU/gram. The data were analyzed by ANOVA. If there was a significance, it would be continued by a DMRT test with an error rate 5%. The research results showed that all extracts concentration of mengkudu fruit and storage-time gave a significant effect (p<0.05) toward bacteria amount. The concentration of 100% had the same activity with formalin to combate the replication of bacteria among 16 hours storage time.

Evaluation of Penicylinders Used in Disinfectant Testing: Bacterial Attachment and Surface Texture

1987 ◽  
Vol 70 (5) ◽  
pp. 903-906
Author(s):  
Eugene C Cole ◽  
William A Rutala ◽  
Johnny L Carson
Keyword(s):  
Stainless Steel ◽  
Surface Texture ◽  
Environmental Protection Agency ◽  
Bacterial Attachment ◽  
Dilution Method ◽  
Bacterial Inoculation ◽  
Protection Agency ◽  
Smooth Numbers ◽  
Salmonella Choleraesuis ◽  
Aoac Method

Abstract Two passible deficiencies in the AOAC use-dilution method for registration of chemical disinfectants by the Environmental Protection Agency are examined: (7) the physical disparities among brands of penicylinders and (2) the variability of bacterial numbers on penicylinders depending upon test strain and penicylinder surface texture. Textual differences of 2 brands of stainless steel penicylinders, one brand of porcelain, and one brand of glass were assessed by scanning electron microscopy. A considerable variation in smoothness of both inner and outer surfaces of stainless steel and porcelain penicylinders was observed. Glass penicylinders were very smooth. Numbers of bacteria attached to a penicylinder were assessed by vortexing the penicylinders 30 s at No. 4 after using the AOAC method of bacterial inoculation and drying 40 min at 37°C. With this methodology, stainless steel carriers retained the 3 AOAC-recommended bacterial test strains differentially: ca 107 for Pseudomonas aeruginosa, 5 x 106 tat Staphylococcus aureus, and 106 for Salmonella choleraesuis; glass retained 10'-107 organisms of all 3 test strains; porcelain retained about that amount of S. aureus but 105-106 P. aeruginosa and 103-104 S. choleraesuis. These data suggest that disinfectants are not similarly challenged with the AOAC-recommended test bacteria and that an alternative method should be considered to ensure comparable numbers of bacteria on penicylinders

Effect of Long-term Storage on Sterile Status of Devices in Surgical Packs

1987 ◽  
Vol 8 (7) ◽  
pp. 289-293 ◽  
Author(s):  
N. Ariene Klapes ◽  
Velvl W. Greene ◽  
Ann C. Langholz ◽  
Cindy Hunstiger
Keyword(s):  
Stainless Steel ◽  
Laminar Flow ◽  
Long Term Storage ◽  
Laminar Flow Hood ◽  
Storage Times ◽  
And Storage ◽  
Term Storage ◽  
Over Time

AbstractWe investigated the effect of the following on the sterile integrity of surgical packs: four wrapping materials (two-ply reusable, nonbarrier wovens, both new and previously used; disposable, barrier nonwovens; and polypropylene peel pouches), dustcovers, two storage locations, and storage times ranging from 2 to 50 weeks. Two hundred sixty-three packs containing stainless steel coupons were prepared, wrapped, sterilized, and stored. Half of the packs were dustcovered prior to storage. At monthly intervals for a year, packs of each type were opened in a laminar flow hood, and the coupons inoculated into trypticase soy broth. The coupon contamination probabilities were 0.019 for reusable, woven packs; 0.017 for disposable, nonwoven packs; and 0.016 for peel pouches. These differences were not significant. The probability of finding a contaminated coupon in any pack after 50 weeks was 0.018. No trend toward increased probability of contamination over time was observed for any of the pack types studied.

Adhesion of Aeromonas hydrophila to Water Distribution System Pipes after Different Contact Times

1998 ◽  
Vol 61 (10) ◽  
pp. 1321-1329 ◽  
Author(s):  
MAFU AKIER ASSANTA ◽  
DENIS ROY ◽  
DIANE MONTPETIT
Keyword(s):  
Stainless Steel ◽  
Surface Energy ◽  
Aeromonas Hydrophila ◽  
Distribution System ◽  
Water Distribution ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Extracellular Materials ◽  
And Storage ◽  
Storage Temperatures

Scanning electron microscopy observation was used to investigate the ability of Aeromonas hydrophila to attach to various water distribution pipe surfaces, such as stainless Steel, copper, and polybutylene, after different contact times at ambient and storage temperatures. Surface energy value of each surface was estimated by contact angle measurements using water, α-bromonaphthalene, and dimethyl sulfoxide. Our results indicated that Aeromonas cells could easily attach to all surface types after exposures as short as 1 or 4 h at both temperatures (4 and 20°C). Polybutylene, a low-energy surface (41.2 mJ-m−2), followed by stainless Steel (65.7 mJ-m−2), was most colonized by Aeromonas cells, whereas few cells were observed on copper, which has a surface energy of 45.8 mJ-m−2. Extracellular materials could also be observed on polybutylene surfaces, especially after 1 and 4 h of exposure at the refrigeration temperature.

Anthocyanin pattern of Tempranillo wines during ageing in oak barrels and storage in stainless-steel tanks

2005 ◽  
Vol 220 (5-6) ◽  
pp. 592-596 ◽  
Author(s):  
Eugenio Revilla ◽  
Jorge F. López ◽  
José-María Ryan
Keyword(s):  
Stainless Steel ◽  
Steel Tanks ◽  
Oak Barrels ◽  
And Storage

scholarly journals Accuracy of partially edentulous arch impressions obtained from different alginates and storage times

2017 ◽  
Vol 15 (4) ◽  
pp. 252
Author(s):  
Marcus Vinicius Loureiro Bertolo ◽  
Lucas de Oliveira Tomaselli ◽  
Rafael Pino Vitti ◽  
Gabriel Abuna ◽  
William Cunha Brandt ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Dimensional Accuracy ◽  
Reference Points ◽  
Mandibular Arch ◽  
Steel Cast ◽  
Storage Times ◽  
And Storage

This study aimed to assess the dimensional accuracy of five commercial alginates verified in stone casts. Methods: Each alginate impression (Cavex ColorChange, Cavex Holland BV; Jeltrate Plus, Dentsply; Hydrogum, Zhermack; Kromopan 100, Lascod; Ezact Kromm, Vigodent) was performed on partially edentulous standard stainless steel mandibular arch cast with reference points on teeth 33, 43, 37 and 47. On the stainless steel cast, the anteroposterior (33-37 and 43-47) and transverse (33-43 and 37-47) distances were measured in a stereomicroscope at 30x magnification and 0.5 μm accuracy. The distances between these points were measured three times, obtaining an average, which was analyzed statistically and compared with the distances obtained from the stone casts. For each alginate the casts were poured gypsum (n = 5) immediately and after a period of 1, 2, 3 and 5 days of the impression procedure. Results: The dimensional accuracy values of stone and stainless steel casts were analyzed statistically by two-way ANOVA and Tukey's test (α=0.05). The results showed significant differences between the alginates; however, no differences in dimensional accuracy were found among the different storage times of alginate impression. Conclusions: It can be concluded that the alginate impressions can be stored for up to 5 days.

Surface conditioning of stainless steel coupons with skim milk solutions at different pH values and its effect on bacterial adherence

Food Control ◽  
2010 ◽  
Vol 21 (12) ◽  
pp. 1769-1773 ◽  
Author(s):  
Nguyen Manh Dat ◽  
Daisuke Hamanaka ◽  
Fumihiko Tanaka ◽  
Toshitaka Uchino
Keyword(s):  
Stainless Steel ◽  
Skim Milk ◽  
Bacterial Adherence ◽  
Surface Conditioning ◽  
Ph Values

scholarly journals Stability of halocarbons in air samples stored in stainless- steel canisters

2020 ◽  
Vol 13 (1) ◽  
pp. 73-84
Author(s):  
Tanja J. Schuck ◽  
Ann-Katrin Blank ◽  
Elisa Rittmeier ◽  
Jonathan Williams ◽  
Carl A. M. Brenninkmeijer ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Storage Time ◽  
Ambient Air ◽  
Sample Collection ◽  
Air Samples ◽  
Upper Troposphere ◽  
Mixing Ratios ◽  
Halon 1301 ◽  
Stable Conditions ◽  
And Storage

Abstract. Measurements of halogenated trace gases in ambient air frequently rely on canister sampling followed by offline laboratory analysis. This allows for a large number of compounds to be analysed under stable conditions, maximizing measurement precision. However, individual compounds might be affected during the sampling and storage of canister samples. In order to assess halocarbon stability in whole-air samples from the upper troposphere and lowermost stratosphere, we performed stability tests using the high-resolution sampler (HIRES) air sampling unit, which is part of the Civil Aircraft for the Regular Investigation of the atmosphere Based on an Instrument Container (CARIBIC) instrument package. The HIRES unit holds 88 lightweight stainless-steel cylinders that are pressurized in flight to 4.5 bar using metal bellows pumps. The HIRES unit was first deployed in 2010 but has up to now not been used for regular halocarbon analysis with the exception of chloromethane analysis. The sample collection unit was tested for the sampling and storage effects of 28 halogenated compounds. The focus was on compound stability in the stainless-steel canisters during storage of up to 5 weeks and on the influence of ozone, since flights take place in the upper troposphere and lowermost stratosphere with ozone mixing ratios of up to several hundred parts per billion by volume (ppbv). Most of the investigated (hydro)chlorofluorocarbons and long-lived hydrofluorocarbons were found to be stable over a storage time of up to 5 weeks and were unaltered by ozone being present during pressurization. Some compounds such as dichloromethane, trichloromethane, and tetrachloroethene started to decrease in the canisters after a storage time of more than 2 weeks or exhibited lowered mixing ratios in samples pressurized with ozone present. A few compounds such as tetrachloromethane and tribromomethane were found to be unstable in the HIRES stainless-steel canisters independent of ozone levels. Furthermore, growth was observed during storage for some species, namely for HFC-152a, HFC-23, and Halon 1301.

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